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Supplemental Digital Content 6

Boxplots of Shannon diversity values calculated from log normalized OTU abundances and the Benjamini-Hochberg corrected p-values of the statistical models. This includes Wilcoxon tests on stress and sex terms and the terms for the mixed linear model. All p-values are Benjamini- Hochberg FDR corrected with each term in the model corrected separately.

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Control Experimental

5.65.86.06.2

Wilcoxon Stress p−value 0.429

Shannon diversity

female male

5.65.86.06.2

Wilcoxon Sex p−value 0.05

Shannon diversity

Shannon diversity at level 1

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Control Experimental

5.65.86.06.2

Stress p−value 0.234

Shannon diversity

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female male

5.65.86.06.2

Sex

p−value 0.0249

Shannon diversity

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Shannon diversity at level 1

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5.65.86.06.2

Stress*Sex interaction p−value 0.0535

Shannon diversity

Control Female

Stress Female

Control Male

Stress Male

5.6 5.7 5.8 5.9 6.0 6.1 6.2

Cage effect p−value 0.802

Shannon diversity

CF1 CF2 SF1 SF2 CM1 CM2 SM1 SM2

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Shannon diversity at level 1

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