Supplementary Table 1 Mass Spectrometry and Database Search Results
Database: nr_20070218 20070218 (464110 sequences; 1602026208 residues) Database searching: MASCOT version 2.2.04.
All Samples analyzed using ABI Q-STAR LC-MS/MS or LTQ-FT (indicated
‡)Spot
number Sequence identification number(s), Primary Protein Name
Protein Molecular Weight
Protein Score
Unique
Peptides Sequence Ion Score m/z
(observed)
Mass (observed)
Mass (theoretical)
Mass Error (ppm)
Modifications
1 1: AAA16820, gi|291922 complement factor B 85450 175 10 VSEADSSNADWVTK 73 754.935 1507.856 1507.679 117
YGLVTYATYPK 59 638.394 1274.774 1274.655 93
FLCTGGVSPYADPNTCR 58 958.023 1914.032 1913.840 100 2 Carbamidomethyl (C)
DISEVVTPR 46 508.338 1014.661 1014.535 124
VASYGVKPR 42 488.830 975.645 975.550 97
DFHINLFQVLPWLK 39 590.730 1769.168 1768.966 114
CLVNLIEK 35 494.808 987.601 987.542 59 Carbamidomethyl (C)
LPPTTTCQQQK 34 651.404 1300.793 1300.645 114 Carbamidomethyl (C)
DAQYAPGYDK 34 564.346 1126.678 1126.493 164
2 1: AAA85332, gi|179665 complement component 3 187046 1360 20 ISLPESLK 48 443.794 885.573 885.517 64
IWDVVEK 38 444.785 887.555 887.475 90
GLEVTITAR 55 480.311 958.607 958.545 65
ISLPESLKR 47 521.855 1041.696 1041.618 74
ADIGCTPGSGK 39 531.291 1060.568 1061.481 861 Carbamidomethyl (C)
VLLDGVQNLR 64 563.871 1125.728 1125.651 69
YYTYLIMNK 59 604.835 1207.656 1207.595 51
EVVADSVWVDVK 38 673.369 1344.724 1344.693 23
TIYTPGSTVLYR 56 685.919 1369.824 1369.724 73
IPIEDGSGEVVLSR 84 735.941 1469.868 1469.773 65
LVAYYTLIGASGQR 93 756.397 1510.779 1510.814 24
TVMVNIENPEGIPVK 80 828.462 1654.910 1654.860 30 Oxidation (M)
SGIPIVTSPYQIHFTK 62 596.718 1787.131 1786.962 94
TELRPGETLNVNFLLR 69 624.754 1871.241 1871.026 114
EYVLPSFEVIVEPTEK 55 939.997 1877.980 1877.966 7
LSINTHPSQKPLSITVR 47 473.569 1890.246 1890.069 94
VVLVSLQSGYLFIQTDK 61 637.435 1909.284 1909.056 119
KVEGTAFVIFGIQDGEQR 44 665.409 1993.204 1993.027 89
VPVAVQGEDTVQSLTQGDGVAK 92 733.423 2197.248 2197.123 57 YFKPGMPFDLMVFVTNPDGSPAYR 52 917.221 2748.640 2748.308 121
3 1: AAB59518, gi|4557325 apolipoprotein E 36131 101 8 SELEEQLTPVAEETR 54 866.030 1730.046 1729.837 121
LGPLVEQGR 52 484.800 967.586 967.545 42
LGADMEDVCGR 50 611.826 1221.638 1221.512 103 Carbamidomethyl (C)
ELQAAQAR 42 443.805 885.595 885.467 145
LEEQAQQIR 40 557.860 1113.705 1113.578 114
WELALGR 34 422.771 843.528 843.460 80
GEVQAMLGQSTEELR 32 824.546 1647.077 1646.793 172
VQAAVGTSAAPVPSDNH 31 810.977 1619.940 1619.790 92
4 1: AAA51743, gi|178741 apolipoprotein C-II 8909 36 1 ESLSSYWESAK 36 643.867 1285.720 1285.583 107
5 1: BAA22651, gi|2521981 alpha2-HS glycoprotein 35641 195 4 AHYDLR 27 387.748 773.481 773.382 128
HTLNQIDEVK 81 598.776 1195.538 1195.620 68
EHAVEGDCDFQLLK 14 554.336 1659.986 1659.756 139 Carbamidomethyl (C) HTFMGVVSLGSPSGEVSHPR 68 694.424 2080.249 2080.016 112
HTFMGVVSLGSPSGEVSHPR 73 699.773 2096.299 2096.011 137 Oxidation (M) HTFMGVVSLGSPSGEVSHPR 9 699.798 2096.373 2096.011 173 Oxidation (M)
gi|136429 Trypsin precursor 23457 153 3 VATVSLPR 52 421.714 841.413 841.502 106
LSSPATLNSR 56 523.338 1044.661 1044.556 100
LSSPATLHSR 8 555.317 1108.620 1109.583 868 Acetyl (N-term)
IITHPNFNGNTLDNDIMLIK 38 767.151 2298.432 2298.168 115 Oxidation (M) Supplemental Table 1
Click here to download Supplemental Data File (doc, pdf, etc.): Wang et al Supplemental Table 1.xls
