عاری‎سازی دو توده تجاری سیر (Allium sativum L.) بومی ایران از پوتی ویروس‎ها با استفاده از گرمادرمانی و کشت مریستم

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E-mail: bahmanzahedi@gmail.com

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REFERENCES

1. Ayabe, M. & Sumi, S. (1998). Establishment of a novel tissue culture method, stem-disc-culture, and its practical application to micropropagation of garlic (Allium sativum L.). Plant Cell Report, 17, 773-779.

2. Bhojwani, S. S., Cohen, D. & Fry, P. R. (1981). Production of virus-free garlic and field performance of micropropagated plants. Scientia Horticulturae, 18, 39-43.

3. Clark, M. F., A. N. Adams, A., D. Voller and E. Bidwell, M. F. (1976). The detection of viruses by enzyme-linked immunosorbent assay (ELISA). Journal of General Virology, 33, 165-167.

4. Conci, V. C., Canqvelli, A., Lunello, P., Di Rienzo, J., Nome, S. F. & Zumelzu, G. (2003). Yeild losses associated with virus – infected garlic plant during five successive years. Plant Disease, 87, 1411-1415.

5. Davis, R. M. (1995). Disease caused by viruses and mycoplasmalike organisms. In: H. F. Schwarts, & S.

K. Mohan, (Eds.). Compendium of Onion and Garlic Diseases. St. Paul. APS Press. p. 54.

6. FAOSTAT data. (2003). http://apps.fao.org/default.htm.Last updated October 2003.

7. Dizaji, I. A. & Izadpanah, K. (2001). Characterization of the virus causing mosaic in Persian leek (Allium ampeloprasum ssp. Persicm Mousavi & Kashi). Iranian Journal of Plant Pathology, 37, 39–49.

(In Farsi).

8. Etemadi, N. (1991). The effect of media and explants in producing garlic plantlet in-vitro. M. Sc. thesis of horticulture sciences, College Agriculture. Tehran University. P.109. (In Farsi).

9. Izadpanah, K. (1982). An annotated list of virus and virus - like diseases of plant in Fars province.

Shiraz University, Shiraz, Iran. (In Farsi).

10. Kamenetsky, R. (2007). Garlic: Botany and Horticulture. In: J. Janick, (Ed.) Horticultural Reviews, Volume 33. (pp. 123 – 172). John Wiley & Sons. Inc.

11. Lunello, P. F., Almonacid, B., Kobayashi, K., Helguera, M., Nome, S. F., Menteaberry, A. & Conci, V.

C. (2000). Distribution of garlic virus A in different garlic production regions of Argentina. Journal of Plant pathology, 82, 17-21.

12. Moriconi, D. N., Conci, V. C. & Nome, S. F. (1990). Rapid multiplication of garlic (Allium sativum L.) in vitro. Phyton, 51, 145-151.

13. Murashige, J. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15, 473-497.

14. Nagakubo, T., Takaichi, M. & Oeda, K. (1997). Micropropagation of Allium Sativum L. (Garlic). In: Y.

P. S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry. (pp. 3-19). (39^th ed.), Springer, verlag.

Berlin- Heidelberg

15. Novak, F. J. (1990). Allium tissue culture. In: H. d. Rabinowitch and J. L. Brewster (eds.), Onions and Allied crops. P. 233-250. Vol. I CRC Press, Boca Raton. Fl.

16. Shahraeen, N., Bananej, K., Ahoonmanesh, A. & Lesemann, D. E. (1998). Identification of three elongated viruses from Allium species (onion and garlic) in Tehran province. Iranian Journal of Plant Pathology, 34(1, 2), 37 – 38. (In Farsi).

17. Ramires- Malagon, R. & Perez-Moreno, L. (2006). Differential organ infection studies, potyvirus elimination, and field performance of virus-free garlic plants produced by tissue culture. Plant Cell Tissue and Organ Culture, 86, 103-110.

18. Ucman, R., Zel, J. & Ravnikar, M. (1998). Thermotherapy in virus elimination from garlic: Influences on shoot multiplication from meristems and bulb formation in vitro. Scientia Horticulture, 73, 193-202.

19. Van der Meer, Q. P. (1997). Old and new crops within edible alliums. Acta Horticulture, 433, 17-31.

20. Van Dijk, P. (1994). Virus disease of Allium species and prospects for their control. Acta Horticulture, 358, 299-306.

21. Verbeek, M., Van Dijk, P. & Van Well, M. A. (1995). Efficiency of eradication of four viruses from garlic (Allium sativum L.) by meristem-tip culture. European Journal of Plant Pathology, 101, 231-239.

22. Voller, A., Bartleh, A., Bidwell, D. E., Clark, M. F. & Adams, A. N. (1976). The detection of viruses by enzyme – linked immunosorbent assay (ELISA). Journal of General Virology, 33, 165-167.

23. Walkey, D. G. A. (1990). Virus diseases. In: H. D. Rabinowitch and J. L. Brewster (Eds.), Onions and allied crops. (pp. 191-212). Vol. II. CRC Press, Boca Raton, Fl.