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Online ISSN: 2382-9931

Journal of Water and Irrigation Management

Homepage: https://jwim.ut.ac.ir/

University of Tehran Press

The Effect of Natural Biochar Application on Nitrogen Fertilizer Productivity in Spinach

Jaefar Nikbakht1 | Shaghayegh Vaziri2 | Taher Barzegar3

1. Corresponding Author, Department of Water Engineering, Agricultural Faculty, University of Zanjan, Zanjan, Iran. E-mail: [email protected]

2. Department of Water Engineering, Agricultural Faculty, University of Zanjan, Zanjan, Iran. E-mail:

[email protected]

3. Department of Horticultural Sciences, Agricultural Faculty, University of Zanjan, Zanjan, Iran. E-mail:

[email protected]

Article Info ABSTRACT

Article type:

Research Article

Article history:

Received 23 November 2022 Received in revised form 18 June 2023

Accepted 24 June 2023

Published online 12 October 2023

Keywords:

Fertigation Fertilizer levels Soil improver

Yield and yield components

Current research was carried out as a pot experiment in order to investigate the effect of different biochar levels (zero (Bi0), one (Bi1) and two (Bi2) percent of soil mass) and nitrogen fertilizer (from the urea source) (zero (F0), 150 (F150) and 300 (F300) kg/ha) on spinach (cv. Viroflay) yield and fertilizer productivity. The experiment was done from September 2021 to January 2022 as a factorial experiment based on randomized complete blocks design with four replications in the research greenhouse of agricultural faculty of Zanjan University. Fertilizing was done as fertigation in three splits with an interval of 10 days. Results showed, using one and two percent biochar were significantly increased means of leaf chlorophyll index 15% and 34% (respectively) and leaf area 14% and 34% (respectively) compared to the control treatment. In F150 and F300 treatments compared to F0, means of leaf chlorophyll index increased 64% and 110% (respectively) and leaf area increased 30% and 58%

(respectively). Based on the results of interaction effects, maximum mean of petiole length, fresh and dry mass of plant and fertilizer productivity were achieved in Bi2-F300 treatment (respectively 6.1 cm, 10.1 gr and 24.0 kg/kg).

Based on the research results, biochar application in soil is suggested to increase fertilizer productivity which reduces the harmful effects of using chemical fertilizers on the environment.

Cite this article: Nikbakht, J., Vaziri, Sh., & Barzegar, T. (2023). The Effect of Natural Biochar Application on Nitrogen Fertilizer Productivity in Spinach. Journal of Water and Irrigation Management, 13 (3), 755-768.

DOI: https://doi.org/10.22059/jwim.2023.351415.1033

© The Author(s). Publisher: University of Tehran Press.

DOI: https://doi.org/ 10.22059/jwim.2023.351415.1033

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Texture

b s OM K Na Ca N EC

(%) (gr.cm3) (%) (mg.kg1) (%) (dS m. 1) pH

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Table 3. Variance analysis of evaluated traits in spinach Source of

variances df Plant Fresh mass

Plant dry mass

Petiole length

Chlorophyll index

Relative water content

Leaf area

fertilizer productivity

Rep 3 0.1ns 0.01ns 0.3ns 9.5ns 33.3ns 7.5ns 0.01ns

Biochar 2 18.1*** 0.2*** 5.9*** 316.2*** 118.3ns 282.8*** 0.8***

Fertilizer 2 77.7*** 0.96*** 27.4*** 1776.3*** 53.0ns 575.3*** 3.4***

Fer×Bio 4 1.8** 0.04* 0.9*** 4.0ns 41.7ns 6.4ns 0.1**

Error 24 0.3 0.01 0.1 4.2 91.3 6.9 0.01

CV (%) - 10.3 15.5 9.2 5.9 14.2 8.5 10.3

ns: no significant; *, ** and ***: significant at 0.1%, 1% and 5% respectively.

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Figure 1. Effect of biochar treatments on chlorophyll index in spinach

Figure 2. Effect of nitrogen fertilizer treatments on chlorophyll index in spinach

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YB[ [ 75 / 1 67 / 1 85 / 1 72 / 1

27 / 2 I I

$(

^ .

Figure 3. Interaction effect of biochar and nitrogen fertilizer treatments on petiole length in spinach

3 - 3 - - 56

W U ) 4 ( R H ` ( c> bB" B ( K B( HI BJi[ f I . ,

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.(

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2% Biochar 1% Biochar 0% Biochar

Petiole length-cm

300N/ha 150N/ha 0N/ha

(9)

` ( B U )

Fallah Morteza Nezhad et al., 2014

E R H B ` ( c> .(

Sadeghi et al.

) (2017

( ( 25

50 100 200

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Abdelraouf

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( ( 56 112 168 224 , ) *B O IB I

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1 / 354 2 / 530 8 / 598 9 / 775 I P( I . U W; D

Figure 4. Effect of biochar treatments on leaf area in spinach

Figure 5. Effect of nitrogen fertilizer treatments on leaf area in spinach

3 - 4 - 8 9:2 ;

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0 10 20 30 40 50

2% Biochar 1% Biochar 0% Biochar

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37.8 a

31.1 b

24.0 c

0 10 20 30 40 50

300N/ha 150N/ha 0N/ha

Leaf area-cm2

(10)

381 e m:I= ; (

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Jasim and Esho, 2022

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$(

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Abdelraouf

) ( 2016

( ( R H N = [ bB" B 168

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$(

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$(

YB[ [ 14 / 83 1 / 6 )

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^

.

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) (2021

$(

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Zibaei et al.

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200

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Figure 6. Interaction effect of biochar and nitrogen fertilizer treatments on plant fresh mass in spinach

Figure 7. Interaction effect of biochar and nitrogen fertilizer treatments on plant dry mass in spinach

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2% Biochar 1% Biochar 0% Biochar

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300N/ha 150N/ha 0N/ha

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(11)

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) ( 2015

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) (2019

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Figure 8. Interaction effect of biochar and nitrogen fertilizer treatments on fertilizer productivity in spinach

(12)

4 -

!=

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5 -

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. A

7 - A "

Abdelraouf, A. A. E. (2016). The effects of nitrogen fertilization on yield and quality of spinach grown in high tunnels. Alexandria Science Exchange Journal, 37, 488-496.

Ahmed, R., Li, Y., Mao, L., Xu, Ch., Lin, W., Ahmed, Sh., & Ahmed, W. (2019). Biochar effects on mineral nitrogen leaching, moisture content, and evapotranspiration after 15N urea fertilization for vegetable crop. Agronomy, 9(331), 1-18.

Bitarafan, Z., Asghari, H., Hasanloo, T., Gholami, A., & Moradi, F. (2018). Biochar effect on seed trigonelline content of fenugreek (Trigonella foenum-graceum L.) ecotypes under deficit irrigation. Iranian Journal of Medicinal and Aromatic Plants Research, 34(1), 155- 165. (In Persian).

Bolhassani, Z., Ronaghi, A., Ghasemi, R., & Zarei, M. (2019). Influence of rice-husk derived biochar and growth promoting rhizobacteria on the yield and chemical composition of spinach in soil under salinity stress. Iranian Journal of Soil Research, 33(3), 335-348. (In Persian).

Charkhab, A., Mojaddam, M., Lack, S., Sakinejad, T., & Dadnia, M. R. (2021). The effect of biochar and humic acid rates on some phophysiological characteristics and grain yield SC704 corn (Zea mays L.) hybrid under water deficit stress. Journal of Crop Ecophysiology, 15(58), 171-192. (In Persian).

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Eskandari, Z., Taab, A., eshghizadeh, H. R., & Khorvash, M. (2021). Growth characteristics and yield evalution of dual-purpose corn hybrids in two levels of urea fertigation. Journal of Crop Production and Processing, 11(3): 111-124. (In Persian).

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Goodarzi, F., Delshad, M., Soltani, F., & Mansouri, H. (2020). Changes in some growth and yield indices of Spinach (Spinacia oleracea L.) under nitrogen fertilization and plant density.

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Hosseinnejad Mir, A., Hashemi Garmdareh, S., Liaghat, A., Karimi, S., & Abbasi, F. (2021). Effect of forage maize biochar and urea fertilizer on soil chemical properties and pepper yield under greenhouse conditions. Water and Irrigation Management, 11(3), 593-606. (In Persian).

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M., & Elkelish, A. (2021). Beneficial features of biochar and arbuscular mycorrhiza for improving spinach plant growth, root morphological traits, physiological properties, and soil enzymatic activities. Journal of Fungi, 571(7), 2-16.

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