微生物 NAD キナ の構造と機能に関する研究 - 日本農芸化学会

NAD

NAD(H) [NAD^ῌNADH]

!"#$%&' ( )*+,-. NAD^ῌ /0)1234^ADP456$ 789:;

<=>$ ?@ABCB? DE,-. F NAD(H)G4;AH-NADP(H) [NADP^ῌNADPH]

?IJ ?-+KL1L?-.NADP(H)

!M ( 79:

N9OPQ E,-.

NAD(H)^NADP(H)?RS+T*

OP NAD(H)/NADP(H)UQRVW-. X UQY?-G NADZ[&\^{(NADK)NADP(H)} ]^9]_`&\[NADP(H)ase]VW-.NADK4;a ^[ATP7344;^(Pn)]K4;b ^NADῌ K4;NADP^ῌKM,-.NADP(H)aseNADP(H) K^{NAD (H)}c4;,-. ATPd^NADK 1950! Pnd^NADK1980!eHfH"gA H*h.ONADP(H)RDOOiPjk#l Kmn o?p$l%gGJ PH*?Oqh. NADP(H)aseE er,P&

VWqh.

'1I NADK^NADP(H)asek#lGsn (AHt)*NAD(H)/NADP(H)+,-?.uv EwK x/)12$ly0OP,-z1 Go2AHh.

1. NADK !"

{|}3Rt~Micrococcus flavus1I Pn^ATP K,-Pn/ATP-NADKK$4 e<056€

7Kz sn^NADKk#l(h. XH1 I ‚0}56ƒ%„8t~ „… )12<&Z<

NADK(?P2^NADKx/p$l

G†?qh.

Bj ,-?9 ‡{|}3Rt~ !ˆ…~^Mycobacte- rium tuberculosis({|}‰Rt~!Escherichia coli,Sphin-

gomonas sp. A1Š( <&Z< !‹`;t~Methanococcus jannaschii(„…9!:Saccharomyces cerevisiae(Œ;

<^NADK+=KŽ* NADK/,+x/7

$>?h4;apb @ R?@Ko h. ,?i ῌ{|}3Rt~<&Z<^NADK Pn^ATPK ^{NAD (H)}K 4 ; , -^Pn/ATP- NAD(H)Z [ & \[NAD (H) K]V W - X ῍„ … NADKATP‘K^NAD(H)K4;,-^ATP- NAD(H)KVW-X ῎{|}‰Rt~^NADK ATP

‘K^NADῌ‘K4;,-^ATP-NADKVW -XK’h ‡4;b @ RT*K“A,-”

@^{NADKNAD (H) K}K•B,-Œ !–¹(.—OD E. coliNADK(YfjB)‘GCD^NADP(H)V<˜9

™4š›EœKb-X )12:^NADKžFŠGGŸ

RK’,X?@D’t)*^NAD(H)ῌNADP(H)UQ +xe+K,-L V?zH%gK h.APNADKF¡x/<|¢;‹;:1IIJK

£^A)12^BKg*¤h. XHP¥ NADKx/

p$lK"LA¦-z1%g?q

*-.

?)Pn§$:4;G4;"9™$ˆMV¨M h©"#$%&MVW-. ATPª-O«¬

V M­^(ADP)G®?* !ATPK*h¯M

­N+^ADPG®-(. eXV ˆ…~^Pn/ATP- NADK(Ppnk)^PnK*h©°D^NADPῌ«¬?+

­OK±² ³­)-^Pn"#$%&a PRKsn’h.

2. NADP(H)ase

Q@ ?^NADP(H)ase´RK’,<$µ4]^9]_

`&\?@%PH*hG NADP(H)4;

1 INAD(H)/NADP(H) U Q O O i - ¶„ · NADP(H)ase&VWqh.

' ) * < & Z <M. jannaschii ; <^NADK

#1 ,-?9;<NADK/,+x/4;apb @ R

$

b¸S¹º» ¼½R¸ 23

(MJ0917)^NADK

^(IMPase)

!"#$ %& IMPase^NADP(H)ase '()*& MJ0917+,-)./01&

IMPase&IMPase2) "3 4++&5

NADP(H)ase678 ^-1,6-9

(FBPase)2) :;<=3/$ MJ 0917>&

NADP^ῌ+- ':2)4?

@NADK^NADP(H)aseA <;3&

NAD^ῌ/NADP^ῌ)BCD'E+FG)H

* ) $ ";=& +I JI+KL

>& NADKM^IMPaseMN

OP+Q)/*6R&IMPase^NADP(H) +S!) T$ U& "+I JI^IMPase M. jannaschii# $^{MJ 0109} Archaeoglobus fulgidus#$^AF23724& IMPase^FBPase%2+V<=

NADP(H)ase2) :)&'$ W+(>&)*

XY)Z++^{IMPaseNADPase} ' ,3[4 T$

3. NADK ^Pn

0ZYPn/ATP-NADK(Ppnk)+I\]] ^NAD^ῌ

+-_ +^X.0/Q ^0-Q_ )`1 ^2²_&

NAD^ῌ0a+3XQb456-78=<<cQ ,d)-$NAD^ῌ0a+e=<<cIf 49g>& :h*+^NADK=B:=;"#*6R& N<Q +Iij<;;<=;^A678^B=

=k* $ U& ]+-=7R& Iij+

l<;>1m3 ;NE/D short motif) ! $ #;+;9g+>& =& Asp189>?@nop qr#$R^2³_& Ppnk+AB-^AῌA῍U>^BῌB῍: 2²_C+nopqrstuD=SE$ NF&Gly187

>& NADK^{NAD (H) K}=6 *=;"#*6R

@NADK, Arg; NAD(H)K, GlyU>vIf4A& ?@no pqr+^NADῌ^=qwxIfyz_0a+{G=

aHI^2³_$NAD^ῌNADHqwxIfyz=6

*+l|3}& ~Ja+If49g^NADK

NAD(H)K+b456-|)`1:Q,d[

T"#$&&a|K|=7R& NADK=6 *>& ~Ja+€L >MNIf4^NADῌ‚ +|)ƒ1:)'$ U& O„…+†P&

~Ja+^GlyU>vIf4‚+¹If4H‡=7 ^NADK<;^NAD(H)K‚+K‡=ˆ$ W+(

<;& NADK=6‰^NADῌ0a+eb456 -|==:& AB-CnopqrstuD+,

)Š=&NADKQB-Q)‹Œ&:3cDR

B-+^NADK;H3 ‹Œ) $

NF&PnSD,Q-+ŽT<;& Pn/ATP-NADKU

=^PnSDViLWXY#$^Pn/ATP-wJ

 +] ^w b4+-_ +0-Q

)`1&Pn0a+Q^PnSD,Q^X!Y]SD,

Q_ );<=$ 36& XY#$wJ )Z +#$‘J’J >U“k ^w +b4F_ )

Z[:& %\+N<Q+] U+}& %J

+Q^F+S+_`>ab+/$ XY#$

Pn/ATP-wJ +0-Q)c}*;<=& % J +Q+S)c}*&'& %J +Q

^F=<<cde+ab=`f)?‰$

O„…>&”ghG^•_ij–G„…kij—lSDm6 78”ghGhG˜nG„…–io+–Gp™+,K‡

Gm=6 *.c#4+$ qršst& šuv)

! ”ghGhG˜›w xyœuz= U

:$ U& O„…=S*šstC!" U”ghGhG

˜{›w žO |z& Q-=6 *šst }RU”ghGhG˜kD~–Gp™›w ŸŸz

=6€ U:$ O„…=š¡‚ !mU\y

„…¢& „…& Gƒ£& ¤¥+¦U„…\+§=

U:$ ¨e=cR©„) !mUª…”ghG†‡

›w ^« ˆ‰¬0hG›w_ ­Š®‹z& ”ghGhG˜

›w ŒO¯Az= U:$ °±=3RU& O

„Ž=šC!" U‘On²FG³S’“d+”N

•z ^”ghGhG˜›w_ 3;8=wŽ–´µ¢+ƒz F=—C6€ U:$

2 Ppnk+0-Q ^˜B-_

T.¶l· AῌA῍U>BῌB῍AB-+nopqrs+

³$

3 Ppnk+NAD^ῌ0a

™¸· qwxIfyz$ Asp189, Gly187 ^¹š

Arg187=H‡_ >?@nopqr=#$$

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