506
1 16DOX
2
3 P450
*4 AGEs
Diospyros kaki
そこで本研究では，抗糖化に着目し，疾病および老化の進行を防ぐことを目的と し摘果柿に含まれる成分のAGEs生成阻害活性について検討したので報告する．
6.33kg 6.50kg
3 NMR, MS
4-(2-O-glucopyranosyl ethyl)phenol [1],Gallic acid [2], Betulinic acid [3] 3
Fig. 1 Structures of isolated compounds
AGEs
Table 1aminoguanidine
3*5 A 42
42
βAβ42
Aβ42 22, 23
.
1)Aβ42
,
2)Aβ42
Aβ42 2
3)(SH-SY5Y)
siRNA
E22P-Aβ42
C Val40
L,
L-2,6-diaminopimelic acid (DAP)
2
3)24
6 SH-SY5Y
MS/MS
5 siRNA
SH-SY5Y SK-N-SH
MTT RNA HuC/D
Aβ42
SH-SY5Y
Aβ42 HuC/D
Aβ42 HuC/D
1) Masuda, Y. et al., ChemBioChem 10: 287-295, 2009.
2) Murakami, K. et al., ACS Chem. Neurosci. 1: 747-756, 2010.
3) Murakami, K. et al., Sci. Rep. 6: 29038, 2016.
Aβ42
*6
*7
、
*8
Saccharomyces cerevisiae
4-deoxy-
L-erythro-5-hexoseulose uronate (DEH) DEH
1)
DEH
2)DEH DEH
DEH
Atu3025 DEH ( )
MOPS (pH 7.0)
Atu3025 DEH
MOPS (pH 8.0) MES (pH 7.0) Atu3025
Atu3025 A1-I
2.3
DEH Atu3025
Rhizopus oryzae
由来グルコアミラーゼのシグナル配列Atu3025 N Atu3025
N
Bae
3)A1-I
1) . (2018) . 2018 . 2A10a14.
2) F. Matsuoka et al. (2017). Sci. Rep. 7, 4206, DOI:10.1038/s41598-017-04481-3.
3) J. H. Bae et al. (2015). Sci. Rep. 5, 12229, DOI: 10.1038/srep12229.
*9 Aspergillus glaucus MA0196
*10 2-hexenal
2018
pH
1.
ATP
ATP ATP
ATP ATP
ITC 2.
3.
in-silico
Biosci. Biotechnol. Biochem. Award review
1 Wakai S et al., Biosci. Biotechnol. Biochem., 68, 2519 2528, 2004 2 Wakai S et al., Biosci. Biotechnol. Biochem., 71, 2735-2742, 2007 3 Wakai S et al., J. Biol. Chem., 288, 29247 29251, 2013
4 Wakai S et al., Environ. Microbiol. Rep., 5, 235 242, 2013 5 Wakai S et al., Microb. Ecol., 68, 519 527, 2014
6 Wakai S et al., Mater. Technol., 30(B1), 38 43, 2015
7 53 8 , 515 520, 2015
8 Wakai S et al., Extremophiles, 21, 471 477, 2017 9 Kuribayashi T et al., Extremophiles, 21, 357 368, 2017 10 Tsurumaru H et al., Sci. Rep., 8, 15149, 2018
WAKAI Satoshi
657-8501 1-1
e-mail [email protected] 2000 3
2005 9
2005 10
2009 4
2013 1
2016 4
2018
Saito, Y., Wanezaki, K., Kawato, A. and Imayasu, S., Structure and activity of angiotensin I converting enzyme inhibitory peptides from sake and sake lees. Biosci. Biotech. Biochem., 58 10 , 1767 1771 1994
512893
2010 134 2010
Kubo, H., Hoshi M., Matsumoto T., Irie M., Oura S., Tsutsumi H., Hata Y., Yamamoto Y., Saito K., Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice.
Lipids Health Dis., 16, 106. doi: 10.1186/s12944-017-0501-y. 2017
5 5474369
TSUTSUMI Hiroko
e-mail [email protected]
日本農芸化学会関西支部第 506 回講演会 幹事校 神戸大学大学院農学研究科 代表： 竹中 慎治 庶務： 山内 靖雄
507
2019 2 2
2018 12 28
2019 1 7 606-8502
E-mail: [email protected] Tel: 075-753-6469 Fax: 075-753-6104
