CHAPTER 4. General conclusions and plans
4.2 Plans for the future
4.2.3 Simultaneous isolation of CTCs and EVs from whole blood on a disc
Although we have focused on the isolation of plasma-driven circulating biomarkers in this thesis due to the fact that they are more reflective of the disease status of cancer in ‘real-time’, CTCs also have particular utility for the diagnosis and treatment monitoring of various cancers. They contain several cellular and subcellular components such as intact DNA, which can be useful for the analysis of well-known mutations or the discovery of novel markers. If we can isolate EVs and CTCs together from an individual blood sample from a cancer patient, combinative studies with downstream analysis can provide valuable clinical meanings for cancer treatment. Therefore, we will develop a lab-on-a-disc system that enables the simultaneous isolation of CTCs and EVs from the same whole blood sample on a single device by integrating modules of CTC isolation and EV isolation in a fully automatic system.
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