INVESTIGATION OF THE EFFECT OF INTERLEUKIN-13 RS20541 (R130Q) SINGLE NUCLEOTIDE POLYMORPHISM IN
ADIPOGENESIS OF MOUSE ADIPOCYTES 3T3-L1
By
TAN HONG JIE
A Project report submitted to Department of Biomedical Science Faculty of Science
Universiti Tunku Abdul Rahman
in partial fulfilment of the requirements for the degree of Bachelor of Science (Hons) Biomedical Science
May 2018
iv ACKNOWLEDGEMETS
First and foremost, I would like to express my sincere appreciation to my supervisor, Dr. Say Yee How for his motivation, aspiring guidance, and practical advices throughout the project. His productive recommendations guided me all the time during the research as well as in the thesis writing. I am also sincerely grateful to my senior master student, Ms. Nor Anis Ahmad for the patience and guidance given throughout the research work. Furthermore, I would like to express my gratitude to laboratory officers, Mr Tie Shin Wei, Mr Gee Siew Meng, and Mr. Saravanan a/l Sivasangaran for their kind assistance and suggestions.
In addition, I would also like to thank my fellow bench mates Png Wen Yang and Chew Yi Ern, who encouraged and supported me throughout the project, and also FYP mates who always wait me or help me for buying lunch and dinner. Besides, I would like to thank to Dr. Say, Mr. Tie and Ms. Anis again for always assisting me in troubleshooting experiments, experimental design and also result analysis.
v DECLARATION
I hereby declare that the dissertation is based on my original work except for quotations and citations which have been duly acknowledged. I also declare that it has not been previously or concurrently submitted for any other degree at UTAR or other institutions.
____________________________
Tan Hong Jie
vi APPROVAL SHEET
This dissertation entitled “INVESTIGATION OF THE EFFECT OF INTERLEUKIN-13 RS20541 (R130Q) SINGLE NUCLEOTIDE POLYMORPHISM IN ADIPOGENESIS OF MOUSE ADIPOCYTES 3T3-L1” was prepared by TAN HONG JIE and submitted as partial fulfilment of the requirements for the degree of Bachelor of Science (Hons) Biomedical Science at Universiti Tunku Abdul Rahman.
Approved by:
___________________________
(Assoc. Prof Dr. Say Yee How) Date:………..
Supervisor
Department of Biomedical Science Faculty of Science
Universiti Tunku Abdul Rahman
vii FACULTY OF SCIENCE
UNIVERSITI TUNKU ABDUL RAHMAN
Date: __________________
PERMISSION SHEET
It is hereby certified that TAN HONG JIE (ID No: 15ADB03716) has completed this final year project entitled “INVESTIGATION OF THE EFFECT OF INTERLEUKIN-13 RS20541 (R130Q) SINGLE NUCLEOTIDE POLYMORPHISM IN ADIPOGENESIS OF MOUSE ADIPOCYTES 3T3-L1” under the supervision of Dr. Say Yee How from the Department of Biomedical Science, Faculty of Science.
I understand that University will upload softcopy of my final year project in pdf format into UTAR Institutional Repository, which may be made accessible to UTAR community and public.
Yours truly,
____________________
(TAN HONG JIE)