SSR Diversity in Darnel Ryegrass and Its Relationship to Fescue Species. (5360)
Authors:
J.C. Zwonitzer* - The Samuel Roberts Noble Foundation
M.A.R. Mian - The Samuel Roberts Noble Foundation, Ardmore, OK M.C. Saha - The Samuel Roberts Noble Foundation, Ardmore, OK Z. Wang - The Samuel Roberts Noble Foundation, Ardmore, OK
Abstract:
Lolium temulentum L. (Darnel ryegrass) is suitable as a model system for forage and turf grasses because it is a self-pollinator with short life cycle, a diploid with no vernalization requirement, and is closely related to major forage and turf species. A study with 41 Lolium temulentum (Lt), three tall fescue, two tetraploid fescue, and two diploid fescue genotypes was initiated using SSRs to assess diversity among these genotypes. A total of 157 tall fescue EST-SSR and 60 Festuca-Lolium genomic SSR primer pairs were screened on a subset of the genotypes. A total of 119 and 110 bands were scored from 10 tall fescue EST-SSR and 12
Festuca-Lolium genomic SSR primer pairs, respectively. Data were analyzed using NTSYSpc 2.1. The Lt genotypes grouped into four major clusters except accession ABY-BA 8892.78 (Lt6) which grouped with the fescues. The Lt6 genotype was grouped closer to meadow fescues than to the other Lt genotypes in the study. Tall fescue, meadow fescue and tetraploid fescue formed their independent clusters. Tall fescue PI423078 was found to be quite different than KY31 and PI297901. The results also indicate that the tetraploid fescues are the most distant from Lolium temulentum. Tall fescue EST-SSRs and Festuca-Lolium genomic SSRs were found suitable for assessing the genetic diversity of Lolium temulentum genotypes. The diversity information will be useful for improvement of the Festuca-Lolium complex.
Speaker Information: John Zwonitzer, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401; Phone: 580-224-6082; E-mail:
Session Information: Wednesday, November 3, 2004, 8:25 AM-11:00 AM Presentation Start: 8:25 AM
Keywords: Lolium temulentum; Genetic diversity; EST-SSRs and genomic SSRs;
Festuca species
