ةرادإ ﻰﻟإ ةدﻮﻋ ﻲﻧوﺮﺘﻜﻟﻻا ﺮﺸﻨﻟا
ﻞﻴﺻﺎﻔﺗ ﺔﻘﻴﺛﻮﻟا
ﻊﻗاﻮﻤﻟا ﺔﺣﺎﺘﻤﻟا : ﺔﻐﻠﻟا
: مﻮﻠﻌﻟا ﺔﻴﻠآ
ﺔﻴﺑﺮﻌﻟا ضﺮﻋ
عﻮﻧ ﺔﻘﻴﺛﻮﻟا :
ﺔﻟﺎﺳر ﺔﻴﻌﻣﺎﺟ
ناﻮﻨﻋ ﺔﻘﻴﺛﻮﻟا ﻲﻓ ﺎﻳﻼﺨﻠﻟ ﻢﺨﻀﻤﻟا سوﺮﻴﻓ ﺔﻴآﺮﺤﻟ تﺎﻴﻨﻘﺘﻟا ةدﺪﻌﺘﻣ ﺔﺳارد :
ةءﺎﻔﻜﻟا يوذ صﺎﺨﺷﻷا
ﻲﻋﺎﻨﻤﻟا ﺺﻘﻨﻟا يوذو ﺔﻴﻋﺎﻨﻤﻟا Kinetics in technical Virological Study of Cytomegalovirus
- Multi
Individuals immunocompetent and Immunocompromised
ﺔﻐﻟ ﺔﻘﻴﺛﻮﻟا :
ﺔﻴﺑﺮﻌﻟا
ﺺﻠﺨﺘﺴﻤﻟا :
سوﺮﻴﻔﻟا ﻞﺜﻤﻳ ﺎﻳﻼﺨﻠﻟ ﻢﺨﻀﻤﻟا
– سﺮﻬﻟا ﺔﻠﺋﺎﻋ ﻦﻣ ﻮهو –
ﻰﺿﺮﻤﻠﻟ سﺮﺷ وﺪﻋ
ﻞﺸﻔﻟﺎﺑ ﻦﻴﺑﺎﺼﻤﻟا ﻰﻠﻜﻟا عرز تﺎﻴﻠﻤﻋ ﺪﻌﺑو يﻮﻠﻜﻟا
. ءاﺮﺟإ ﻰﻟإ ﺔﺳارﺪﻟا ﻩﺬه فﺪﻬﺗو
ﻦﻴﺑ ﺔﻧرﺎﻘﻣ ﺔﻴﻟﺎﻌﻟا ﺔﻗﺪﻟﺎﺑ ﻊﺘﻤﺘﺗ ﻲﺘﻟا ﺔﻴﻨﻘﺘﻟا ﻰﻟإ لﻮﺻﻮﻟاو ﺔﻴﻠﻤﻌﻣ تﺎﻴﻨﻘﺗ ﺔﺛﻼﺛ
ﻲﺳوﺮﻴﻔﻟا ءﺐﻌﻟا سﺎﻴﻗو سوﺮﻴﻔﻟا ﻦﻋ ﻒﺸﻜﻟا ﻲﻓ ﺔﻋﺮﺴﻟاو ﺔﻘﺋﺎﻔﻟا ﺔﻴﺳﺎﺴﺤﻟاو ﻲﻓ
ﻰﺿﺮﻤﻟا مد تﺎﻨﻴﻋ .
ﺔﻨﻴﻋ نﻮﻌﺒﺳو ﺔﻴﻧﺎﻤﺛو ﻦﻴﺘﺋﺎﻣ ﻊﻤﺟ ﻢﺗ ﺪﻗو .
ﻞﺼﻣ ﺪﻗو ﺎﻣزﻼﺑو
ﺔﻌﺑرأ ﻰﻟإ ﺎﻬﻔﻴﻨﺼﺗ ﻢﺗ نأ ﺪﻌﺑ ﺔﺣﺮﺘﻘﻤﻟا تﺎﺳارﺪﻠﻟ ﺖﻌﻀﺧأ ﻲﻟﺎﺘﻟﺎآ تﺎﻋﻮﻤﺠﻣ
:
ﺔﻄﺑﺎﻀﻟا ﺔﻋﻮﻤﺠﻤﻟا :
ﻦﻣ ﺔﻨﻴﻋ نﻮﺴﻤﺧو نﺎﻨﺛا ﺖﻠﻤﺷ وأ مﺪﻟﺎﺑ ﻦﻴﻋﺮﺒﺘﻤﻟا ءﺎﺤﺻﻷا
ﻰﻠﻜﻟا . ﺔﻴﻧﺎﺜﻟا ﺔﻋﻮﻤﺠﻤﻟا :
نﻮﺘﺳو نﺎﻨﺛا ﺖﻠﻤﺷ ﺔﻴﺋﺎﻬﻨﻟا ﻞﺣاﺮﻤﻟا ﻲﻓ ﻰﺿﺮﻣ ﻦﻣ ﺔﻨﻴﻋ
ﻞﻴﺴﻐﻠﻟ نﻮﻌﻀﺨﻳ ﻦﻳﺬﻟا يﻮﻠﻜﻟا ﻞﺸﻔﻠﻟ ﺐﺳﺎﻨﻤﻟا عﺮﺒﺘﻤﻟا رﺎﻈﺘﻧا ﻲﻓ ﻢﻈﺘﻨﻤﻟا يﻮﻠﻜﻟا
.
ﺔﺜﻟﺎﺜﻟا ﺔﻋﻮﻤﺠﻤﻟا :
ﺖﻠﻤﺷ ﻢﻬﻟ ةﺪﻳﺪﺟ ﺔﻴﻠآ ﺔﻋارز ﺪﻌﺑ ﻰﺿﺮﻤﻟا ﻦﻣ ﺔﻨﻴﻋ نﻮﻌﺒﺳو ﺔﻌﺴﺗ
، ﺔﻨﻴﻋ نﻮﻧﺎﻤﺛو ﺔﺴﻤﺧ ﺖﻠﻤﺷ :ﺔﻌﺑاﺮﻟا ﺔﻋﻮﻤﺠﻤﻟا ﺔﻳﺮﻳﺮﺴﻟا ﺔﻟﺎﺤﻟا ﺐﺴﺣ ﻢﻬﻤﻴﺴﻘﺗ ﻢﺗ ﺪﻗو ﻰﺿﺮﻣ ﻦﻣ ﻢﻈﺘﻨﻤﻟا يﻮﻠﻜﻟا ﻞﻴﺴﻐﻠﻟ نﻮﻌﻀﺨﻳ ﻦﻳﺬﻟاو ﻦﻣﺰﻤﻟا يﻮﻠﻜﻟا ﻞﺸﻔﻟا
. ﺖﻣﺪﺨﺘﺳا
ﺔﻴﻟﺎﺘﻟا ﻞﻴﻟﺎﺤﺘﻟا ﻞﻤﻋ ﻰﻓ ﻰﺿﺮﻤﻟا ﻞﺼﻣ تﺎﻨﻴﻋ :
- • مﺎﺴﺟﻷا ﻦﻋ ﻒﺸﻜﻟا ةدﺎﻀﻤﻟا
ماﺪﺨﺘﺳﺎﺑ ﻦﻣﺰﻤﻟاو دﺎﺤﻟا ﺎﻬﻴﻋﻮﻨﺑ ﺎﻳﻼﺨﻠﻟ ﻢﺨﻀﻤﻟا سوﺮﻴﻔﻠﻟ ﺔﻴﻤﻳﺰﻧﻹا ﺔﻴﻋﺎﻨﻤﻟا ﺔﺴﻳﺎﻘﻤﻟا
) اﺰﻴﻟﻹا .(
• ﻲﺛارﻮﻟا ﺾﻤﺤﻟا ﻦﻋ ﻒﺸﻜﻟا ءﺐﻌﻟا سﺎﻴﻗو ﺎﻳﻼﺨﻠﻟ ﻢﺨﻀﻤﻟا سوﺮﻴﻔﻠﻟ
ﻰﺿﺮﻤﻟا ﺎﻣزﻼﺑ ﻲﻓ ﻲﺳوﺮﻴﻔﻟا يوﻮﻨﻟا ﺾﻤﺤﻟا ﺮﻴﺒﻜﺘﻟ ةﺮﻤﻠﺒﻟا ﺔﻴﻨﻘﺗ ماﺪﺨﺘﺳﺎﺑ
. • ﻒﺸﻜﻟا
تﺎﻨﻴﺠﻴﺘﻧأ ﻦﻋ ﺎﻳﻼﺨﻟا عراﺰﻣ ﻲﻓ ﻰﺿﺮﻤﻟا ﻞﺼﻣ تﺎﻨﻴﻋ ﻦﻘﺣ لﻼﺧ ﻦﻣ سوﺮﻴﻔﻟا
ﺔﺒﺳﺎﻨﻤﻟا . ﻊﺘﻤﺘﺗ ﻲﺳوﺮﻴﻔﻟا ءﺐﻌﻟا سﺎﻴﻘﻟ ﺔﻴﻤﻜﻟا ةﺮﻤﻠﺒﻟا ﺔﻴﻨﻘﺗ نأ ﺞﺋﺎﺘﻨﻟا تﺮﻬﻇأ ﺔﻗﺪﻟﺎﺑ
ﻲﺳوﺮﻴﻔﻟا ءﺐﻌﻟا ﻦﻣ ﺔﻔﻠﺘﺨﻤﻟا تﺎﻳﻮﺘﺴﻤﻟا ﻦﻋ ﻒﺸﻜﻟا ﻲﻓ ﺔﻴﺳﺎﺴﺤﻟاو ﺔﺒﺴﻨﺑ
١٠٠ %
ةﺮﻣز ﻲﻋﺎﻨﻤﻟا ﻢﺴﺠﻟا ﻦﻋ ﻒﺸﻜﻟا رﺎﺒﺘﺧا ﺮﻬﻈﻳ ﻢﻟ ﺎﻤﻨﻴﺑ IgM
ﻊﺑرأ ﻊﻣ ﻻإ ﺔﻴﺑﺎﺠﻳإ
ﻂﻘﻓ ﻢﻬﻨﻣ تﻻﺎﺣ .
نأ ﻰﻠﻋ ﻊﻃﺎﻗ ﻞﻜﺸﺑ ﺞﺋﺎﺘﻨﻟا ﺖﻟد ءﺐﻌﻟا سﺎﻴﻘﻟ ﺔﻴﻤﻜﻟا ةﺮﻤﻠﺒﻟا ﺔﻴﻨﻘﺗ
ﺔﻴﺳﺎﺴﺤﻟاو ﺔﻗﺪﻟﺎﺑ ﻊﺘﻤﺘﺗ ﻲﺳوﺮﻴﻔﻟا ﻦﻋ ﺮﻜﺒﻤﻟا ﻒﺸﻜﻟا ﻲﻓ ﺎﻬﻴﻠﻋ دﺎﻤﺘﻋﻻا ﻦﻜﻤﻳو
ﻊﻴﻤﺟ ﻲﻓ ﺔﻴﺳوﺮﻴﻔﻟا ﺔﺑﺎﺻﻹا ﻲﻋﺎﻨﻤﻟا زﻮﻌﻟا ﻰﺿﺮﻣ تﺎﻨﻴﻋ
، ﺔﻴﻠﻤﻌﻤﻟا ﺞﺋﺎﺘﻨﻟا ﻩﺬه نأو
ﺔﻘﻓاﻮﺘﻣ ﺖﻧﺎآ ﻰﺿﺮﻤﻠﻟ ﺔﻴﻜﻴﻨﻴﻠآﻹا ﺔﻟﺎﺤﻟا ﻊﻣ مﺎﺗ ﻖﻓاﻮﺗ
.
فﺮﺸﻤﻟا :
أ . د . رﻮﺼﻨﻣ أ ﻲﻨﻴﺠﺳ ﻞﻴﻤﺟ .
د . ﻲﺘﺷاﻮﻗ ﺔﺗﺎﺤﺷ ﺎﻬﻣ
ﺔﻨﺳ ﺮﺸﻨﻟا :
٢٠٠٨ ـه
ﺦﻳرﺎﺗ ﻊﻗﻮﻤﻟا ﻰﻠﻋ ﺔﻓﺎﺿﻻا :
Wednesday, July 02, 2008
Document Type : Thesis
Document Title : Multi-technical Virological Study of Cytomegalovirus Kinetics in immunocompetent and Immunocompromised Individuals
ةءﺎﻔﻜﻟا يوذ صﺎﺨﺷﻷا ﻲﻓ ﺎﻳﻼﺨﻠﻟ ﻢﺨﻀﻤﻟا سوﺮﻴﻓ ﺔﻴآﺮﺤﻟ تﺎﻴﻨﻘﺘﻟا ةدﺪﻌﺘﻣ ﺔﺳارد ﻲﻋﺎﻨﻤﻟا ﺺﻘﻨﻟا يوذو ﺔﻴﻋﺎﻨﻤﻟا Document : Arabic
جوﺮﺧ ةﺪﻋﺎﺴﻣ ﺮﻳرﺎﻘﺘﻟا ﻊﻗﻮﻤﻟا ﺔﻤﻈﻧأ
Language
Abstract : Human cytomegalovirus (HCMV) is a serious pathogen for immunocompromised individuals. The present study aimed at providing the best choice of three laboratory techniques for CMV detection regarding to sensitivity, specificity and time-
consumption. Detection of Anti-CMV IgM and IgG, viral load determination by quantitative PCR, and shell-vial tissue culture for CMV antigenaemia.Two-hundred and seventy-eight serum and plasma samples have been collected and categorized into four groups : (I) comprised fifty-two apparently healthy blood donors and kidney donors as a control group,(II) comprised sixty-two end stage renal disease patients,(III) comprised seventy-nine post- kidney transplant patients under immunosuppressive and cytotoxic therapy, as they were subdivided according to their clinical presentation into three subgroups: A, B, C ,(IV) comprised eighty-five patients suffering from chronic renal failure. Results revealed that quantitative PCR detected markedly high viral load (4000 to > 100.00 DNA copy/mL) in sixteen kidney transplant recipients, whom presented with clinical
invasive CMV disease 16/16 (100%), while only 4/16 (25%) were detected positive for IgM anti-CMV, another ten patients with symptoms of CMV syndrome were found CMV-PCR positive with low viral (436-3070 copy/mL) and negative IgM anti-CMV.
On the other hand, no CMV viral load was detectable among end- stage renal disease patients, although 6/62 (9.7 %) were found positive for IgM anti-CMV. All studied cases and controls were positive for IgG anti-CMV because of the high carrier rate of CMV almost 90%. All 26 positive CMV PCR samples were negative for shell-vial . Based upon these results, quantitative PCR provides very sensitive and reliable assay for early monitoring of CMV active infection in immunocompromised patients.
Publishing Year : 2008 AH
Added Date : Wednesday, July 02, 2008
نﻮﺜﺣﺎﺒﻟا
ﻢﺳا ﺚﺣﺎﺒﻟا
) ﻲﺑﺮﻋ ( ﺚﺣﺎﺒﻟا ﻢﺳا
) يﺰﻴﻠﺠﻧا ( ﻢﺳﻻا ﺮﻴﺧﻷا ) ﻲﺑﺮﻋ ( ﺮﻴﺧﻷا ﻢﺳﻻا
) يﺰﻴﻠﺠﻧا عﻮﻧ (
ﺚﺣﺎﺒﻟا ﺔﺒﺗﺮﻤﻟا
ﺔﻴﻤﻠﻌﻟا ﺪﻳﺮﺒﻟا
ﻲﻧوﺮﺘﻜﻟﻻا ناﻮﻨﻌﻟا
) ﻲﺑﺮﻋ ناﻮﻨﻌﻟا (
) يﺰﻴﻠﺠﻧا (
يزﺎﻏ ءﺎﻨﺳ سﺎﻄﻌﻟا Sanaa Ghazi
Al-Attas ﺚﺣﺎﺑ
