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Diagnostic Bacteriology 2 Course Code: CLSC 326 Program

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Course Title: Diagnostic Bacteriology 2 Course Code: CLSC 326

Program: Bachelor of Clinical Laboratory Sciences

)Sakaka - Qurayyat(

Department: Clinical Laboratory Sciences ) Sakaka - Qurayyat (

College: Applied Medical Sciences

Institution: Jouf University

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Table of Contents

A. Course Identification ... 3

6. Mode of Instruction (mark all that apply) ... 3

B. Course Objectives and Learning Outcomes ... 3

1. Course Description ... 3

2. Course Main Objective ... 3

3. Course Learning Outcomes ... 4

C. Course Content ... 4

D. Teaching and Assessment ... 5

1. Alignment of Course Learning Outcomes with Teaching Strategies and Assessment Methods ... 5

2. Assessment Tasks for Students ... 5

E. Student Academic Counseling and Support ... 5

F. Learning Resources and Facilities ... 6

1.Learning Resources ... 6

2. Facilities Required ... 6

G. Course Quality Evaluation ... 7

H. Specification Approval Data ... 7

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A. Course Identification

1. Credit hours: 3 (2+1+0) 2. Course type

a.

University

College

Department Others

b.

Required Elective

3. Level/year at which this course is offered: Level 6 \ 3

rd

Year

4. Pre-requisites for this course

(if any)

: Diagnostic Bacteriology 1 (CLSC 325) 5. Co-requisites for this course

(if any)

: None

6. Mode of Instruction (mark all that apply)

No Mode of Instruction Contact Hours Percentage

1

Traditional classroom (Lectures and Practical) 4 100%

2

Blended

3

E-learning

4

Distance learning

5

Other

7. Contact Hours (based on academic semester)

No Activity Contact Hours

1

Lecture 30

2

Laboratory/Studio

30

3

Tutorial

4 Others

(specify)

Total 60

B. Course Objectives and Learning Outcomes 1. Course Description

Diagnostic Bacteriology 2 emphasizes the correlation of in vitro and in vivo findings in the diagnosis and treatment of infectious disease caused by Gram

ve bacteria, their diseases, modes of transmission, clinical presentation, pathogenesis, and laboratory diagnosis, susceptibility testing, prevention, and controls. This will include medically important Gram

ve bacteria including Neisseria, Enterobacteriaceae, Pseudomonads, Vibrios, Campylobacter, Helicobacter, Bordetella, Brucella, and Haemophilus. The students will also get knowledge about Mycoplasma, Chlamydia, and spirochetes.

2. Course Main Objective

 Understand the concepts of different Gram –ve bacterial infections and spirochetes.

 List the virulence factors and pathogenesis of Gram –

ve bacterial infections and spirochetes.

 Describe the laboratory diagnosis of medically important Gram –

ve bacteria and spirochetes

 Perform appropriate laboratory techniques in processing of clinical specimens,

identification of Gram

ve bacteria and to evaluate antimicrobial sensitivity.

 Evaluate and interpret the test results with the patient presentation.

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3. Course Learning Outcomes: By the end of the course the student will be:

CLOs Aligned PLOs

1 Knowledge and Understanding

1.3

Recognize the Gram-negative bacteriological infections and their effects occurring in the human body.

Recognize the normal organs functions and mechanisms of various pathological conditions precisely.

2 Skills

2.3

Use advanced and state of the art equipment for analyzing the microbiological specimens and bacteria.

Use accurately advance and smart devices for analyzing the clinical specimens.

3 Values

3.2

Perform personal integrity, respect, honesty, and Islamic ethical behavior when dealing with colleagues, patients, community members, and the hospital staff.

Perform personal integrity, respect, honesty and Islamic ethical behavior when dealing with patients, Community members and the healthcare team.

C. Course Content:

No List of Topics Contact

Hours

1 Pathogenicity, transmission, and lab diagnosis of Neisseria meningitidis and

Neisseria gonorrhoeae. 2

2 Enterobacteriaceae: General characteristics, the medical importance of members of this group of organisms. Lactose fermenting (LF) bacteria (E. coli, Citrobacter, Klebsiella, Enterobacter) and Serratia, their diseases, pathogenesis, modes of transmission, antibiotic susceptibility, cultural characteristics, growth identifications, prevention, and control.

4

3 Non-Lactose fermenting (NLF) medically important Enterobacteriaceae members (Salmonella, Shigella, and Proteus), their diseases, pathogenesis, transmission modes, antibiotic susceptibility, cultural characteristics, growth identifications, prevention, and control.

2

4 Gram-negative Non-fermentative bacteria; Pseudomonads (Pseudomonas aeruginosa, fluorescens, putida, luteola, Burkholderia cepacia, Burkholderia pseudomallei, Stenotrophomonas maltophilia, and Sphingomonas paucimobilis) and Non-Pseudomonads (Acinetobacter baumannii and Moraxella).

4 5 Pathogenicity, transmission, and lab diagnosis of Vibrio species. 2 6 Pathogenicity, transmission, and lab diagnosis of Campylobacter and

Helicobacter. 2

7 Pathogenicity, transmission, and lab diagnosis of Yersinia species. 2 8 Pathogenicity, transmission, and lab diagnosis of Haemophilus and other

HACEK organisms. 2

9 Pathogenicity, transmission, and lab diagnosis of Bordetella, Brucella, and

Pasteurella. 2

10 Legionella and Gardnerella, their medically important species, characteristics,

diseases they cause, and lab diagnosis. 2

11 Spirochaetes; Treponema species, characteristics, species of medical importance, diseases they cause, clinical presentation, pathogenesis, modes of transmission, serological diagnosis of syphilis, antibiotic susceptibility, prevention, and control.

2

12 Spirochaetes: Borrelia, Leptospira - characteristics, species of medical importance, diseases they cause, clinical presentation, pathogenesis, modes of transmission, antibiotic susceptibility, prevention, and control.

2

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Total 30

No. List of Topics (Practical)

1

Introduction to lab safety in a medical bacteriology lab

2

2

Media used in isolation of Gram-negative bacteria

2

3

Gram staining

2

4

Biochemical Test – Citrate Utilization test

2

5

Biochemical Test – Methyl Red test

2

6

Biochemical Test -Voges-Proskauer test

2

7

Biochemical Test -Indole test

2

8

Biochemical Test -Urease test

2

9

Biochemical Test – Oxidase test

2

10

Triple Sugar Iron Agar

2

11

Biochemical identification tests for Enterobacteriaceae

4

12

The API 20E System

2

13

Antimicrobial Susceptibility Testing for various Gram-negative bacteria

4

Total 30

D. Teaching and Assessment

1. Alignment of Course Learning Outcomes with Teaching Strategies and Assessment Methods

Code Course Learning Outcomes Teaching Strategies

Assessment Methods 1.0

Knowledge and Understanding

1.3

Recognize the Gram-negative bacteriological infections and their effects occurring in the human body.

Interactive lectures Written exams

2.0

Skills :

2.3

Use advanced and state of the art equipment for analyzing the microbiological specimens and bacteria.

Practical work Practical exams 3.0

Values:

3.2

Perform personal integrity, respect, honesty, and Islamic ethical behavior when dealing with colleagues, patients, community members, and the hospital staff.

Group projects Presentations

2. Assessment Tasks for Students

# Assessment task* Week Due

Percentage of Total

Assessment Score

1 First Mid. Term Exam (Written exam) 6 7.5%

2 Second Mid Term Exam (Written exam) 11 7.5%

3 Presentation 5 5%

4 Practical exam 15 30%

5 Final Exam 16 50%

Total 100%

*Assessment task (i.e., written test, oral test, oral presentation, group project, essay, etc.)

E. Student Academic Counseling and Support

Arrangements for the availability of faculty and teaching staff for individual student

consultations and academic advice :

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6 office hours and 6 hours for academic advice per week.

F. Learning Resources and Facilities 1. Learning Resources

Required Textbooks

1. Review of Medical Microbiology and Immunology 14th Edition, Warren Levinson, McGraw-Hill Education, 2016.

2. District Laboratory Practice In Tropical Countries (Part 2) 2nd Edition, Monica Cheesbrough, Cambridge University Press, 2006.

3.

Color Atlas & Textbook of Diagnostic Microbiology, 7th Edition. Koneman’s, Lippincott Williams and Wilkins, 2016.

Essential References Materials

1. Journal of Bacteriology

2. Antimicrobial Agents and Chemotherapy 3. Clinical Microbiology Reviews

Electronic Materials

1. http://www.microbiologybook.org/book/bact-sta.htm

2. https://www.cdc.gov/ncezid/dhcpp/bacterial_special/index.html 3. https://www.asm.org/

4. https://microbiologysociety.org/why-microbiology-matters/what-is- microbiology.html

Other Learning Materials

1. Multi-Media and relevant websites and online resources 2. https://www.microbes.info/

3. http://microbiologyonline.org/about-microbiology/introducing-microbes 4. https://www.asm.org/

5. Medical Microbiology and Immunology (Application)

2. Facilities Required

Item Resources

Accommodation

(Classrooms, laboratories, demonstration rooms/labs, etc.)

- Classroom (with at least 25 seats with multimedia) - laboratories (Microbiology Lab)

Technology Resources

(AV, data show, Smart Board, software, etc.)

- Computer - Data show - Smart Board

- Network for technology transfer.

Other Resources

(Specify, e.g. if specific laboratory equipment is required, list requirements, or

attach a list)

-Nil

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G. Course Quality Evaluation Evaluation

Areas/Issues Evaluators Evaluation Methods

Student evaluation of the quality of

learning experience in the program Students Indirect:

Questionnaire

Auding by another faculty members Faculty member Direct:

Report

Evaluation areas (e.g., Effectiveness of teaching and assessment, Extent of achievement of course learning outcomes, Quality of learning resources, etc.)

Evaluators (Students, Faculty, Program Leaders, Peer Reviewer, Others (specify) Assessment Methods (Direct, Indirect)

H. Specification Approval Data

Council / Committee

Departmental Council

Reference No.

9

Date

4\4\2021

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Appendix:

Learning outcome assessment- Diagnostic Bacteriology 2 (CLSC 326) Alignment of course learning outcomes with its of assessment tasks for Diagnostic Bacteriology 2 (CLSC 326)

Assessment tasks Midterm 1 Midterm 2 Presentation Practical Exams

Final Exam

NQF learning domains and learning outcomes for CLSC 326

1.3 √ √ √

2.3 √

3.2 √

Distribution of assessment grades for Diagnostic Bacteriology 2 (CLSC 326)

Assessment tasks Midterm 1 Midterm 2 Presentation Practical Exams

Final exam TOTAL

NQF learning domains and learning outcomes for CLSC 326

1.3 7.5 7.5 50 65

2.3 30 30

3.2 5 5

TOTAL 7.5 7.5 5 30 50 100

* (NQF) National Qualifications Framework

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CLSC Program learning Outcomes Mapping Matrix

Align the program learning outcomes with program courses, according to the following desired levels of performance (I = Introduced P = Practiced M = Master)

Levels

Course code

& No.

Program Learning Outcomes Knowledge and

understanding Skills Values

K1 K2 K3 K4 S.1 S.2 S.3 S.4 V.1 V.2 V.3

3

PHYS 211 I P I

ANA 221 I P I I

CLSC 211 I I I

CLSC 212 I I I

CLSC 251 I I I

CLSC 252 I I I

4

CLSC 221 I P I

CLSC 222 I I I

CLSC 241 I P I

CLSC 242 I I I

CLSC 213 I I I

5

CLSC 321 P P P

CLSC 322 P P P

CLSC 311 P P P

CLSC 323 P P P

CLSC 341 P P P

CLSC 324 P P P

CLSC 325 P P P

6

CLSC 331 P P P P

CLSC 332 P P P

CLSC 351 P P P P

CLSC 352 P P P P

CLSC 326 P P P

CLSC 312 P P P

7

CLSC 411 M M M M

CLSC 431 M M M M

CLSC 432 M M M

CLSC 451 M M M M

CLSC 441 M M M

CLSC 412 M M M M

8

CLSC 452 M M M

CLSC 433 M M M M

CLSC 434 M M M M

CLSC 413 M M M M

CLSC 421 M M M M

CLSC 422 M M M M

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