KHOA HPC CdNO NGHt
IXIGHIEIV CLTLI CAI THlElXI GIEIV KlI^IVI TRA P H A M CHAT TREIV HAT LLIA Oru^a sativa L. THOIVG QUA
[\iLiDi CAY TLII PHAIM
Nguyen Tlii Lang', VO Thj T r i My', TVdn Tlii Thanh XA', Biii Chi Buu=' T6MTAT
Kha nSng nu6i ciy bao ph^n khic nhau d ^ g k^ giua cic loAi liia lai, MOt qudn th^ c6 don b^i k^p dupe thi^t l§p th6ng qua nu6i c^y bao p h ^ cua m^t sd gi6ng liia lai Fl trong ni6i truing N6. Moi truing nay to ra a^c bi^t thich hpp d^ nu6i cfiy bao phfin Iiia lai indica. Y^u td c6 tAm quan trpng nha't trong qud trinh nudi cSy bao ph&i Xk su khac nhau v^ ki^u gien. Nhung k^t qua nghifin ciiu cung cho th£iy nhiing gidng lua thu6ng ho$c lua lai bi^u hi§n r^t khAc nhau trong qud trinh nudi ciy bao phfln. Mst khdc, mot truing nu6i c^y ciing c6 anh huong Ion d^n qui trinh nu6i c^y bao p h ^ . Nhung k^t qud thu duf^c tir td hpp lai IR 64/OM 3536, OMCS 2000/Jasmine M dd cho thAy lodi ndo c6 ty 1§ md s?o cao hon. Nhiing k^t qua do rat cd ich doi v6i v i ^ chpn gidng bd, m? ddp img cao yfiu cdu cua qud trinh nudi Cciy bao p h ^ nhdm nuoi cay Ilia don bdi va thiet l^p q u ^ th^ Itia don bOi k^p b^n vung. C6 6 td hpp lai Fl: IR64/OM 3536, IR 72/OM 3536, OM 3536/lR 73689-76, OM 3536/IR75997-159, OMCS 2000/Jasmine M vd Tequng/OM3536 cho ndng suAt va chdt lupng gao cao.
Tir khod: Don bdi kdp, gidng lua, nudi cdy bao phdn.
L BAT VAN K
San xuat cac ddng dem boi ( doubled haploid - DH) th6ng qua nuoi cay tiii phfc ( anther culture) cho phep phat trien nhanh ckc dong dong trgi, qua do nit ngan thoi gian chpn giong thay vi thdng qua phuong phap co truyen phai mat tilr 6 den 7 th^ h^.
DH con dimg de danh gia c^c tinh trang do gien lan (recessive traits) Idem soat hay tao su dot bien (mutant) hoac lai (hybridization) (Lang va ctv, 2004, 2007). Di^u ki&n chSm soc cac to hop lai, viec thu dong, moi truong nuoi cay co anh huong den su hinh thanh md seo sau khi c^y dong nhat. Cdc tiii p h ^ tu
; con Iai Fj cua cac to hop co su khac nhau v i ti I^ % hinh thanh mo seo trdn mdi trudng khdc nhau. Diiu , nay cd th^ dupe giai thich do phan ung ciia cac ki^u J gien trong cac con lai khdc nhau Id khdc nhau trong Cling dieu kidn mdi tiirong. Diiu ndy phii hgp voi cdc I nghidn ciiu khing dmh kiiu gien la quan trgng nhat J cho su cam ung thanh lap md seo khi nudi cay tiii I phan (Nguydn Thi Lang vd ctv, 2005).
' Do v|iy, de tai " Nghien cthi cai thien gien
* pham chi't trdn hat Ma Oryza sativa L thong qua nudi cay tiliphaiT dugc thuc hidn n h ^ phuc vu cho cdng tac chgn gidng co pham ch^t com tot
LVATlfUVAr
'Vifn Lua DBSCL
^ Vifn Khoa hpc KTNN mien Nam
1. V$t li$u
Gdm cdc gidng liia dugc thu tai ngan hang gien cua Vidn Liia ddng bang sdng Cuu Long.
2. Phuong phdp
Nudi cay tiii ph&, khao sat su hmh thdnh va phat trien, kha nang tai sinh cua md seo theo quy trinh cua Lang, 2002 va cd cai tiin nhu sau:
Hat F l ciia cdc to hgp lai liiy tir ngan hang gien cua Vien Lua DBSCL dugc gieo lam hai dgt trong nhd ludi d i thu tui phan.
- Mau la bdng lua non dugc thu vdo khoang 7-9 gid sang, khi khoang each tir gdc Id ddng den gdc la thli nhat tir 5-10 cm, rCra sach sau dd giu am trong qua trinh xu ly lanh d 10"C trong 7 -10 ngay.
- Tir ddng liia sau khi dugc xu ly lanh, lay nhirng hat d giua bdng cd dau trdn ciia tiii phan chua vugt qud 1/2 chiiu dai hat liia. H^t liia non dugc xir ly vd triing bing each ngam trong cdn 70% trong 1 phut, sau dd ngam trong dung dich thuy ngan clorua 0,1%
thdi gian 15 phiit. Rua sach mdu cay 3 ldn bang nudc c^t vo triing truoc khi dua vao nudi cay.
- Tlii phan dugc tdch ra khdi vo trau bang each diing keo vd kep da vd triing cat dau hat lua trong tii cay dat ldn dia petri da vd triing, dimg kep giir dau hat Ilia roi gd nhe tren midng binh cho hii ph& roi vd trdi deu trin mdi trudng nudi cay tao md seo. Thi
N 6 N G NGHllP VA PHAT TRIEN NONG THON - KY I - THANG 10/2012
KHOA HQCCdNONOHl
nghiim du<?c l^p lai 4 ldn, m6i ldn city 5 binh, mdi binh c^y 150 tui phdn. M^u sau khi c^y dugc giQ trong tu tdi d nhiit do 25"C.
- Md s?o hinh thdnh cd kich thudc 2-3 mm dugc cay chuyin vdo mdi tnrdng tdi sinh d i t^o cSy (tgo cum chdi). Cdc cum chdi (cdy xanh) dugc nhdn v6 tinh trdn mdi trudng nhdn chdi, b diiu kiin 25''C, 10 gid chiiu sdng trdn ngdy ddm bing 02 bdng ddn huynh quang 40 W dat trin b i mdt binh nudi cdy each 30 cm.
- Sau khi cd dii sd lugng chdi c ^ thiit, c^y chuyin sang binh tam gidc d i nudi riing trong mdi trudng MS khdng c6 chat diiu hod sinh trudng. Giu cdc binh cdy b 25"C. 10 gid chiiu sdng trdn ngdy dim bang 02 bdng din huynh quang 40W ddt trdn b i mdt binh nudi cay cdch 30 cm.
- Mdt niia sd cdy trong cung ddng dugc tao r i trdn mdi trudng tao rd, mdt niia sd cdy cdn Igi sd dugc giu trong phdng. Cdy cd r i phdt triin dugc thich nghi trong dung dich Yoshida trong diiu kiin nhi^t dd phdng 3-5 ngdy trudc khi chuyin cdy ra trong trong nha ludi.
Cdc mdi trudng dugc sir dung trong nudi cay tiii phan Ilia:
Mdi trudng tao md seo: N6 + 2 mg/l 2,4D + 60 g/lit sucroza; mdi trudng tdi sinh: MS +1 mg/l BAP + 1 mg/l NAA + 1 mg/l Kinetm + 30 g/1 sucroza ; mdi trudng nhan chdi: MS + 2 m g / l BAP + 30 g/1 sucroza; mdi trudng nhan nhanh: MS + 30 g/1 sucroza; mdi trudng tao rd: MS +1 mg/l NAA + 30 g/1 sucroza; mdi trudng thich nghi: dung djch Yoshida.
Ket qua thu nhatr.
Sd tlii phan dtfdc nudi cay; sd md seo dugc tao thanh; sd md seo dugc cay chuyin; sd cum chdi tdi sinh cay xanh.
TVdng cdy tdi sinh (cdy Al) trong nhd ludi - Cac cdy liia cd rd phdt triin dugc thich nghi trong dung dich Yoshida et al. (1976) b thi nghiim 1 duoc trdng ridng theo timg ddng trong thi nghiim 2.
Thi nghidm dugc lap lai 3 ldn, moi ldn trdng 20 cdy.
- Cac ddng cho hat lep (ddng vd tinh cdn giii Igi trong phdng) se dugc xu ly Conchixium ndng dd 0,05
% trong 10 phiit trudc khi trong ra nhd ludi.
- Thdi gian sinh trudng (ngay): dugc tinh tir khi thich nghi trong dung dich Yoshida ( 1976) d i n khi sd h?t trin bdng chin 85%.
- Chiiu cao cdy (cm): dugc tinh tCr gdc cdy s^
m^t 6.it din chdp bdng, do chiiu cao ciia 10 cdy.
- Sd bdng /byi (bdng): dim s6 bdng /lO bui, tiri|
Irung binh.
- Sd hgt chiic /bdng (hgt): d i m todn bd sd h?
chic/bdng cua 5 byi, tinh trung binh.
- Tj' li lip (%): d i m todn bd sd hat lip/bdng ck 5 bMi, tinh trung binh, sau dd chia cho tdng s^
hgt/bdng (chdc vd lip) x 100%.
- Khdi lu(;mg 1000 hgt (g): Cdn 3 ldn khdi lugng cua 500 hat, tinh trung binh x 2.
- Ndng sudt ly tiiuyit - (Sd hgt chlc/bdng x s6 bdng/byi x TL.1000 hgt x 33 bui) x l a ^
- Ndng su^t cd t h i (g/cdy): cdn sd hgt chdc ciia 10 bgi, tinh trung binh.
- Ty l i cdy khdc dgng (%): d i m sd cdy c6 ki^u hinh khdc dang/ tdng sd cdy cung ddng dugc trdng.
M-K^QUAvATHiblLUAN
1. Chgn Igc gidng p h i m chit thdng qua nudi c^
tlii phdn
Nudi c^y tiii phdn Id phuong phdp chung di tao cay c6 t i bdo don bdi. Phirong phdp ndy giiip chpn ra nhiiu ddng mdi vdi cai tiin tinh trgng nong hpc ciia gidng nay. Trong nghiin cuu cay hii phin d i cdi tieb pham chat trdn cdc gidng liia, tdp trung cdc tinh trgng chinh: phim chit com vd phim chat dinh dudng ciia hat gao.
Nudi ciy tiii phin trong t h i h i Fi vd F; cua cSy Ilia d i tgo qudn t h i t i bao don bgi vd don bgi kep kiem sodt bdi cac yiu td tham gia nhu mdi truong nudi cdy, diiu kiin tdi. Viic nudi cdy t i i ph& Uianh cdng tuy thudc vao cdp lai.
24
Hinhl: Till phin hinh thdnh md s ^ sau 4 tdin dugc dua vdo mdi trudng nudi d y Tuy tiieo Kmg gidng cd t h i tii 4 d i n 8 h i n thi md seo (callus) xuit hiin. Phin trSm md seo xuat hiin dugc ddnh gid tu^ tiiudc vdo phin trdm ciia t^
N 6 N G NGHlfP VA PHAT TRI^N N 6 N G T H 6 N - KY 1 - THANG 10/20«
KHOA HQC C 6 N 0 HOHt
phia sin xu^t ra callus. Khi callus dii 2 mm, s6 dirpc
chuyfa sang m6i tmemg tti sinh. D^ d^m phan tram ta sinh, d^m so callus tir tiii phii t^io ra cSy xanh v4 ca c4y bach lang tr6n 100 tili phSn dui?c phSn 14p.
Nhan c4y tii sinh tu mflt callus tjo ra du<?c tr6ng ra ngoii dit vi cho ciy phit tri&.
Tir mo s?o chuyfo sang mdi tru6ng tii sinh cho ti 16 cao til 4391 tCi td hpp lai IR64/OM3536. Tuy nhiSn cung c6 nhi^u cip lai khong cho thiy li 16 tii sinh nhu Tequing/OM 3536, Thii Lan/DS 20, v.v... Khi dinh gii ciy tii sinh tir moi tnr6ng nu6i ciy tui phin cho thay td hpp cho ti \i ciy xanh cao nhit li IR64/OM 3536 (189i), ki S6 li c?p lai OMCS 2000/Jasmine85.
Ciy md lua nhim muc dich sin xuit ciy don bdi hoic ciy luOng bpi kdp thdng qua sv nhin ddi nhifm sic th^ o md sf o don bdi di ciy hii phin. Cic bidn di Ihirdng g}p va dupe bidt thdng qua nudi ciy tiii phin.
Bidn di niy tao ciy phit tridn khdng binh thucmg nhu bach tang, ciy mdo md, lim, v.v... Do dd gia ting bidn dj ciia gidng cay trdng cung li mdt phin
quan hpng trong cdng tic cii Udn ciy liia. Iflii nghidn ciiu ciu tnic td bio ciia li ciy bach tang cho thiy khdng cd ribosom, do dd qui hinh sinh tdng hpp protein cua ciy bach t?ng bj inh huong dan ddn tinh hang khdng miu trdn ciy vi li vi khdng hinh thinh cic tidn luc lap.
Khi nudi ciy ciy hai Ii mim, hidn hipng chuydn hT/c tidp tir Udu bio tit ndn it thiy xuit hidn ciy bach tsng. Ddi vdi ciy mdt li mim nhu liia, ciy hoin chinh vi phit Uidn phii qua giai dojn md seo thi ti Id bach tang chidm ti Id khi cao, ti Id niy cd khi ldn ddn 5M0%. Didu niy phii hpp vdi cic kdt qui cua Lee, hudng hpp niy din ddn ciy tii sinh rit thip nhu td hpp OM 1490/MTL 250 ti 1^ bach t?ng chidm 98,2%, Tim Thom/OM 3536 (96,4«) hdn bang 1. Didu niy CQng phii hpp vdi mdt sd td hpp cd 85% ciy bach tang nhu td hpp OM 4392/Jasmine85. Thdng Ihuong cic td hpp cho ciy bach tang nhidu thi t^ Id cic ciy hdng dupe dua ra nhi ludi rit thip do ti Id sdng sdt khdng
Bing 1; Dinh g i i ti 1^ md seo vi
C5plai
OM 1490/HoaLai (Huong Lii) IR64/ OM 3536 IR72/OM3536
OM3536/IR73689-76 OM3536/IR75997-159 OMCS2000/Jasmine85 OM1490/KhaoDawMalil05 Om 1490/Soc Trang 3 OM 1490/Soc Trang 5 OMCS 2000/JasmmeM OM 1490/MIL 250 SdcNiu/ OM 3536 TamThom/OM3536 Tequmg/OM3536 Thailan/DS20 Types/DS 20 SdcNiu/ jasmine 85
Sd hii phan
480,00cd 678,00b 491,00cd 500,00c 250,00f 240,00f 456,00cde 800,00a 753,00a 450,00cde 500,00c 420,00de 420,00de 420,00de 500,00c 500,00c 500,00c
Tlidi gian hinh thinh md seo (ngay)
3 2 4 4 5 5 6 5 5 4 6 7 8 4 6 6 5
phit tridn ciy
Tyldmd seo(%)
52,00e 56,70bc 47,49f 54,00cde 44,20£K 41,10g 23,20h 17,80i 56,40b«
52,30de 55,60b-e 55,40b-e 42,30K 44,10fe 65,60a 59,30b 56,40b-e
tii sinh trdn td hpp lai
Tyld ciy xanh (%)
3,12de 18,23a l,50fgh 0,75hi 7,02b 7,30b 5,20c l,20Khi 4,60c 2,30efe 0,150i l,20ghi 2,70ef 2,60e£g 2,30e(g 2,40efg 2,50efg
Tyld ciy tii
sinh
17,81b 43,75a 4,50h 4,50h 14,20c 10,20d 14,50c 7,20fe 8,90de 5,60h 2,10i 2,40i 4,60h 0,20j 0,40j 5,60h 7,60ef
Tyld cay bach tang
(%)
48,43i 35,00k 53,58h 85,00de 83,50e 84,50de 85,60de 91,20b 87,50cd 77,90f 98,20a 90,50bc 96.40a 65,20g 66.50g 67,90g 84,20de
IONG NGHlfP VA PHAT TRIEN N O N G THON - KY 1 - THANG 10/2012
25
KHOA HpC C 6 N 0 N 0 H |
TimThom/ Jasmme 85 Tequing/ Jasmine 85 Thailan/ Jasmine 85
CV,%
LSD 0,05
500,00e 500,00c 500,00c 8,30 6,64
7 7 7
- -
55,70b-e 56,80bcd 57,90bc
4,81 1,57
4,30cd 4,70c 5,60c 18,5 0,48
5,90Kh 7,90ef 9,50d 9,37 0,55
46,90ij 44,50i 44,70j 2,79 U I Kdt qui b bing 1 ghi nhin dttpc mdt sd ddng cd
khi nSng tii sinh ciy xanh cao, nhu cic td hpp lai:
IR64/ OM 3536 (43,75%), OM 1490/HoaLai (Huong Lii) (17,81%), OM3536/IR75997-159 (14,20%).
'Binh gii chit luvng ctia cic ddng nuii dy mi Kdt qui dua ra so sinh vi quan sit ngoii rudng li 32 ddng, chpn so sinh so khdi li 20 ddng. Tir 20
nghidm.
Kdt qui phin tich ANOVA cic ddng td bio soma cho thiy phim chit ciia cic gidng dat him lupng amyloza trung binh, dd b^ic bMng cip 0. Dinh gii ning suit vi thinh phin ning suit ciia cic ddng cit thdi gian ngin tii 81- 88 ngiy, diy li cic gidng cd uii Uld dd luin canh baVu trong nim.
Bing 2: Cic die tinh hinh thii ciia cic ddng nudi ciy Uii phin tir td hpp lai Al - Vlj ddng xuin 2011
Stt 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Cip lai OM 1490/HoaLai (Huong Lii) IR64/ OM 3536
IR72/ OM 3536 OM 3536/1R73689-76 OM 3536/IR75997-159 OMCS2000/Jasmine85
OM1490/KhaoDawMali 105 OM 1490/Soc Trang 3 OM 1490/Soc Trang 5 OMCS 2000/ Jasmine M OM 1490/MTL 250 SdcNiu/ OM 3536 TimThom/ OM 3536 Tequing/ OM 3536 Thailan/ DS 20 Type3/ DS 20 SdcNiu/ jasmme 85 TimThom/ Jasmine 85 Tequing/ Jasmine 85 Thailan/ Jasmine 85
CV,%
LSD 0,05
TGST 84,00hi 81,00i 87,00gh 88,00fKh 100,00c 102,00bc 100,00c 90,00£g 92,00ef 95,00de 98,00cd 105,00b 100,00c 100,00c 100,00c 100,00c 95,00de 100,00c 110,00a 95,00de 2,50 1,58
Cao ciy 68,60h 74,00K 79,40f 80,00f 110,00a 100,00c 100,00c 90,00e 95,00d 98,00cd 104,00b 110,00a 112,00a 110,00a 111,00a 113,00a 112,00a 114,00a 110,00a 112,00a 2,35 1,54
Dii bdng 21,03K 20,96K 20,27g 23,28f 28,20a 24,60ef 25,00de 27,00abc 26.00be 26,50bcd 26,40bcd 26,10b-e 25,30def 24,60ef 25,50def 25,40de£
27,20ab 24,60ef 25,70c-f 23,40f
2.93 0,48
N 6 N G NGHlfP VA PHAT TRIEN N 6 N G T H 6 N - Ki' 1 - THANG 10/2012
KHOA HQC CdNO N G H |
Tiip tue nghiin cuu ndng sufit vd chpn ra bdn ddng cd ndng sudt tir 5 t ^ / h a . Tuy nhiin khdi lugrng
1000 hat ciJa nhung ddng ndy rdt thdp vi hat rat ngdn vd nhd.
Stt 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Ngudn gdc OM1490/HoaLai (HuongLai) IR64/ OM 3536
IR72/ OM 3536 OM3536/IR73689-767 OM 3536/IR7599-159 OMCS 2000/Jasmine85
OM1490/KhaoDawMali 105 OM1490/SocTrang3 OM1490/SocTrang5 OMCS 2000/Jasmine M OM 1490/MTL 250 SdcNau/OM 3536 TimThom/OM 3536 Tequing/OM 3536 Thailan/DS 20 Type3/DS20 SdcNiu/Jasmine 85 TamTliom/Jasmine 85 Tequing/Jasmine 85 Thailan/Jasmine 85
CV,%
LSD 0,05
Sd bdng/m^
321,00e 356,00cd
456,00a 457,00a 456,00b 356,00cd 366,00cd 358,00cd 359,00cd 365,00cd 365,00cd 369,00c 354,00cd 345,00cde 346,00cd
321,00e 352,00cd 256,00f 342,00de 344,00cde 3,74 8,85
Sdh?t chic/bdng
123,00d 142,00c 145,00bc 156,00a 95,00i 98,00ghi
96,00hi 100,00£gh
102,00Jg 103,00(8 104,00f 156,00a 147,00b 120,00d 123,00d 156,00a 120,00d 103,00fe 114,00e 120,00d 2,12 1,69
Tyld ldp 12,00cd 10,00efg 12,00cd ll,00def 12,00cd 10,00efg 5,00j 9,00gh 8,60gh 3,60k 8,20hi 5,60j 9,60fgh
7,10i ll,20de 12,10cd 13,40c 15,60b 16,70ab 17,50a 8,05 0,56
TLlOOOhat (g) 25,60ab
26,50a 26,50a 26,80a 26,90a 27,00a 26,30a 26,50a 25,40ab 25,60ab 26,50a 26,40a 26,50a 26,30a 25,70ab 25,30ab 24,50b 26,50a 25,70ab 26,80a
3,35 0,58
Ning suit c r / h a ) 6,60a-d 6,50a-d 5,60cde 5,60cde 5,90cde 5,60cde 6,40bcd 6,80a<l 6,90a-d 7,30abc 7,90ab 8,20a 8,10ab 8,10ab 7,90ab 5,70cde 5,20de 5,40de 5,30de 4,60e 13,89 0,59 Phdn tich ndng sudt cua cdc ddng trdn ghi nhdn
cdc ddng cho ndng sudt kha hem so vdd gidng gdc ban dau. Diiu ndy chiing td can lua ch^n cdc ddng thuan d i phuc vu trong sdn xudt tuong lai tai cdc tinh.
3. Ddnh gid phdm chAt cdc ddng nudi ciy hii phdn h i Ihu 2011
Nudi cdy tiii phdn cho tan sd biin dj cao hon nudi cdy t i bdo soma. Tuy nhidn cdn phai lua chpn bd m? cho phii hpp vdi cdc muc tidu mong mudn, thi du nhu cdc td hpp IR64/ OM 3536, IR 72/ OM 3536...
Cic ddng Cao cay
(cm) Sdchdi Dii hat (cm)
Hat ldp/
bdng H?t chic/bdng
TGST (ngiy)
KL 1000(gr) NS(gr) IR64/OM3536
1 2 3
100,00d-g 101,00d-g 102,00def
15,00ab 12,00de ll,00ef
7,10ab 7,10ab 7,30ab
ll,00ef 14,00c 12,00de
146,00f 156,00e 145,00ef
105,00a 95,00cde 95,00cde
26,50abc 26,50abc 26,30abc
23,10cd 23,10cd 25,30b
N 6 N G NGHllP VA PHAT TRIEN N 6 N G T H 6 N - KY 1 - THANG 10/2012 27
KHOA Hgc C 6 N 0 NOHt
*
1 2 3 4
1 2 3
103,00de
123,00b 145,00a 100d,0O-g
120,00b
105,00d 102,00def
96,00g 14,00bc
13,00cd 14,00bc 12,00de 15,00ab
14,00bc 10,00f 12,00de
6,90a 1 13,00cd | 175,00c IR72/ OM 3536 7,10ab
7,20ab 7,30ab 7,10ab
19,00a 9,00g 12,20de 13,20cd
156,00e 147,00f 156,00e 125,00g OM 3536/1R73689-76 7,20ab
7,10ab 7,05a
ll,20ef 16,50b 14,20c
156,00e 156,00e 241,00a
96,00cd
90,00cde 90,00ef 90,00ef 85,00f
85,00f 90,00ef 94,00de
1 24,20e
25,60b-e 26,40abc 25,601>e 2 5 , 6 0 b ^
2 6 , 5 0 a b c 26,40abc 26,50abc
24,20bc
•
23,20cd 21,30ef 23,40cd 16,801ii
19,20g 15,60(1 17,40h OM 3536/IR75997-159
1 2 3 4 5
98,00e{g 97,00ig 90,00h 100,00d-g 102,00def
12,00de 13,00cd 14,00bc 10,00f ll,00ef
7,20a 7,10ab 7,10ab 7,20ab 6,58a
10,30fg 14,20c 10,03{g ll,20ef ll,40et
98,00h 75,00i 102,00h 103,00h 195,00b
100,00b 98,00cd 97,00cd 98,00cd 95,00cde
26,30abc 26,40abc 25,60b-e 25,90a<i 26,80ab
20,10(g 23,10cd 23,10c(i 22,50de 23,40cil Teqmng/ OM 3536
1 2 3
121,00b 98e,00fe 100,00d-g
8,00g ll,00ef 16,00a
7,20ab 7,10ab 7,00a
12,00de 10,20fg 14,20c
125,00g 156,00e 148f
100,00c 95,00cde 90,00ef
2 5 , 0 0 c d e 27,50a 24,50de
41,20a 15,30) 14,50]
OMCS 2000/ Jasmine M 1
2 3 4 CV,%
LSD 0,05 100,00d-g
110c lOOd-g 98etg
2,74 1,74
12,00de 14bc 12de 15ab 6,02 0,46
6,80a 7,lab 7,5a 7,2a 12,35 0,53
lO.OOfg 14c 12de lief 5,99 0,45
165,00d 174c 172c 145e£
2,54 2,29
94,00de 95cde 100c 100c 2,98 1,72
27,30a 2 5 , 6 b <
26,5abc 26,5abc 3,27 0,52
21,10f 41a 23cd 25,2b 3,47 0,48 Dinh gii phim chit cua c i c ddng tridn vpng tir miii thom cao nhu: OM 3536/1R75997-159 vi IR 64/
nudi ciy hii phin OM 3536.
Cd 5 td hpp dupe dinh gii tdt trong nudi ciy Uii phin phuc vu chpn tao gidng, Uong dd cd td hpp cho
Cic ddng
1 2 3 4
Amyloza (%)
23,20bcd 22,10de 19,20gh 19,80fgh
B i n g 5 : P h i m d i Bdn gel (mm)
56,30h 55,40h 74,20de 77,40ab
i t dia c i c ddng tir nudi ciy tlii phin Protein (%) |Trcr hd (cip) | Miii Oiom (cip) IR64/ OM 3536
7,20cd 6,50d 7,80iHi 8,50abc
3 5 5 5
1 1 0 1
.»
Bac b u n g ^
l i 1
0 ! 1 0
N 6 N G NGHlfP VA PHAT TRIEN N 6 N G THON - KY 1 - THANG 10/201'
KHOA HQC C O N O N O H t
IR72/ OM 3536 1
2 3 4
20,30fg 23,10cd 22,10de 23,40bcd
75,20cd 69,20g 74,20de 73,20e
8,60abc 8,40abc 8,50abc 7,90a-d
5 5 5 5
0 0 0 0
0 0 1 1 OM 3536/IR73689-76
1 2 3
20,30fg 25,40a 24,60ab
74,20de 41,20k 45,60i
7.20bcd 7,30bcd 7,60a-d
5 3 3
0 0 0
0 0 1 OM 3536/IR75997-159
1 2 3 4 5
24,30abc 21.20ef 23.50bcd
23,10cd 24.50abc
46,30i 76,50bc 74,50de 71,20f 45,30i
8,60abc 8,90a 8,70ab 8.50abc 8.60abc
3 5 5 3 5
0 2 1 2 0
1 0 1 0 1 Tequing/ OM 3536
1 2 3 4
24.30abc 22,10de 20,10fgh 19,20gh
43,50j 67,90g 75,60cd 77,20ab
8,50abc 8,20abc 8,40abc 8,30abc
3 5 5 5
0 2 0 0 OMCS 2000/ Jasmine M
1 2 3 4 CV,%
LSD 0,05 17,80i 19,50gh 20,10fgh 18,70hi 3,60 0,46
77,50ab 74,50de 78,30a 74,20de
1,21 0,47
8,20abc 8,10abc 8,60abc 8,40abc 9,75 0,47
5 5 5 5
0 0 0 0
1 0 1 1
1 1 1 1
n. XET LUAN
Tit ci cic td hpp lai dua vio nudi ciy Uii phin ddu cd khi nang hinh thanh md seo va tii sinh cSy, tuy nhidn cic td hpp lai khic nhau thi ti Id tii sinh ciy xanh cung khic nhau.
Qua quan sit vi dinh gia kidu hinh cua ciy Al trong nhi ludi, tat ca cac ddng nudi ciy Uii phin dupe tao ra rat tdt vd pham chit so vdi gidng gdc . Cic ddng dupe tap ra qua nudi ciy Uii phin thi tuong ddi ddng nhit, chi cd mdt ddng cd su bidu hidn ciia ciy khic dang. Cd mdt sd ddng thudc td hpp IR 64/OM 3536, IR 72/ OM 3536, OM 3536/ IR 73689-76, OM 3536/ IR 75997-159, OMCS 2000/ Jasmine M vi Tequng/ OM 3536 cho ning suit ci thd khi cao va
cic dac tinh ndng hpc tuong ddi tdt. Cic ddng niy cin tidp tue dupe chpn lpc dd dua vio sin xuit.
TMlCUIIUMKilAO
1. Chu C. C, 1978. The N6 medium and its appUcaUon to anther culture of cereal crops. In: Proc Symp on Plant Tissue Culture. Science Press, Beijing, China, pp 43-50.
2. Nguydn Thi Lang, 2002. Phuong phip co bin trong nghidn ciiu cdng nghd sinh hpc, NXB Ndng nghidp, U-. 63-72.
3. Nguyen Thi Lang, Dang Minh Tam, Hhomi Kobayashi and Bui Chi Buu, 2005. Invitro selection
N 6 N G NGHlfP vA PHAT TRIEN N 6 N G THON - KV-1 - THANG 10/2012
29
KHOA HQC C 6 N 0 NOHt
of somaclonal and gametoclonal variants for salt gamatolonal trdn td hpp liia lai. Tap chi Ndng nghii tolerance in rice. Rice is Life, IRRI AND Japan, 15. v i PTNT 3+4 : 25-28.
4. Nguydn Thi Lang, Nguydn Thi Xim, Trjnh 5. Yoshida S., D. A. Fomo, J. H. Cock and K Hoing Khai v i Biii Chi Biiu, 2007. Khai thic bidn di Gomez, 1976. Laboratory manual for physiologic
studies of rice. IRRI, Los Banos, Philippins.
STUDY ON IMPROVEMENT OF QUALH-ATIVE GENE IN THE GRAIN OF RICE (Oiyza sativa L) VIA ANTHER CULTURE
Nguyen Thi Lang, Vo Thi Tra My, T^an Thi Thanh Xa, Bui Chi Buu Summary
Anther culturability of rice is signilicantly different between crosses rice varieUcs. A doubled haploid (DH) population was estabbshed vi.i anther culture of some crossing from Fl on N6 medium, which had been shown particularly suitable for anther ciillurr of indica hybrids. Among these factors, the most important one is the genotypic difference. The results found that different rice species, or crosses quite differendy in response to anther culture. On the other hand, culture medium also strongly influences the andier culture response. The results that IR 64/OM 3536. OMCS 2000/ Jasmine M were which had high callus information rate. The results may be useful in the selection of parents with high response to anther culture for rice haploid breeding and in die establishment of permanent DH populations. There are 6 crosses Fl IR 64/Om 3536. IR 72/ OM 3536. OM 3536/ IR 73689-76. OM 3536/ IR 75997-159. OMCS 2000/ Jasmme .M and Tequng/ OM 3536 give generation good for yield and also quality.
Keywords; Doubled haploid, rice variety, anther culture.
Ngudi phin bidn: GS.TSKH. Trin Duy Qu^
Ngiy nhin bii: 31/5/2012 Ngiy flidng qua phin bidn: 26/9/2012 Ngiy duydt dang: 4/10/2012
N 6 N G NGHlfP VA PHAT TRI^N N O N G T H 6 N - K^" 1 - THANG 10/2012
