TUUNG QUAN TY SO PROTEIN/CREATININ TRONG NUAC TIEU NUA.
von PROTEIN NIEU 24 GIO 0 MOT SO BENH THAN MAN TINH
T O M T A T
Diiu tra dich t§ hoc gan diy cho thiy bdnh ly than man dang tdng Idn nhanh chdng va trd thanh mdt van d4 y te toan cau Nhieu cdng trinh nghidn cuu vd each tiep can chi so ndng dd protein nidu 24 gid la sCf dung ty' so pmtein/creatinin nieu 6 miu turdc tiiu ngau nhidn, hai chi s6 nay tuang quan rat chdt che cd thi dung di thay the ch! so ndng dd protein nidu trong mau nudc tiiu 24 gid Doi tuang nghl&n cUU' g6m 82 ngudi duac chin doan xac dinh viem du than man, suy than man (vidm cau than man. 29 bdnh nhan. suy than man giai doan II: 27 benh nhan. suy than man giai doan III: 26 bdnh nhan). Ket qua va ban luan Ty so P/C d miu nudc tiiu ngiu nhien moi tuong quan thuan rat chdt che vdi ndng dd protein nidu 24 gid- (r = 0.985:
p < 0.001); tuang quan giOa 2 chi so nay khdng phu thupc vao tuoi. gidi. thi tich nudc tiiu. Tuang quan giUa protein nieu 24 gid va t^ so P/C d mau nudc tiiu ngiu nhidn khdng phu thudc vao mite loc cau than.
Tirkhda: benh ly than man SUMMARY
Recent epidemiological investigations showed that chronic kidney disease is increasing rapidly and become a global health problem. Many research works on how to approach the 24-hour urinary protein concentration index is to use the ratio of protein / urinary creatinine in random urine samples, two indicators very closely correlated index can be used to replace urinary protein levels in 24-hour urine sample.
Research subjects: including 82 who are diagnosed with chronic glomerulonephritis, chronic renal failure (chronic glomerulonephritis. 29 patients,chronic renal failure stage II: 27 patients, chronic renal failure stage III: 26 patients). Results and discussion: The ratio of P / C in random unne samples very tight correlation with 24-hour urinary protein levels, (r - 0985. p <0.001):
comelation between the two indicators is not dependent on age, sex, unne volume. Con-elation between 24-hour urinary protein and the P / C in random urine samples is not dependent on the glomerular filtration rate.
Keywords: chronic kidney disease.
DAT VAN
Bi
D i e u tra d i c h t d h o c t r o n g n h t i n g n a m g a n d a y c h o thay b e n h ly t h a n m a n d a n g t a n g l e n n h a n h c h d n g v a trd thanh m d t van d e y t e l o a n c a u . T h e o n g h i e n c i f u N H A N E S - i n c u a M y c d n g b d n a m 2 0 0 7 ty le b e n h t h a n man trong cdng d d n g la 1 3 % h o a c eCf 1 0 nguPi s e e o 1 ngydi benh than m a n tinh. 6 V i e t N a m tuy ehua c d s d lieu thdng ke O J the v d s d ngudi bi S T M , nhung theo Nguydn 7>ii Thinh v a c d n g s y thi trong s d hon 2 2 5 0 bdnh nhan dieu tri ndi trii tai k h o a T h a n - Tidt nieu
P H A N T H ! H A L I N H - Dai hoc Y khoa Vmh H U Y N H Q U A N G T H U A N , P H A M V A N T R A N
B^nh vidn 103
b e n h v i e n B a c h M a i t y 1 9 9 1 d e n 1 9 9 5 , d a c 6 d e n 4 0 . 4 % b e n h n h a n bi s u y t h a n (ca c a p v a m a n l i n h )
O e p h a t h i e n , d i i p h o n g c a c b e n h ly ve t h a n c u n g n h u I h e o d d i , d a n h gia h i e u q u a d i d u t n . l i e n t n e n c u a b e n h t h a n co rat n h i e u toai x e t n g h i e m nhu" xet n g h i e m n u d c l i e u , m a u , s i e u a m h e l i e l n i e u , n h U n g xet n g h i e m n u d c l i e u ( c u t h e la x e l n g h i e m p r o l e i n ) la m o t xet n g h i e m n h a n h , d o n g i a n , t h u a n l i e n va h i e u q u a k i n h t e , d o d o n d dUOc f h u c h i e n tai h d u h e t c a c c o s d y te,
T r e n t h e g i d i d a c 6 n h i e u c o n g trinh n g h i e n c i r u v e e a c h tiep c a n c h i s d n d n g d o p r o t e i n n i e u 2 4 g i d la sir d u n g ty so p r o t e i n / c r e a t i n i n n i e u a m a u nUdc l i e u n g S u n l i i e n , hai c h i s d n a y t i i d n g q u a n rat c h a t c h e co t h e dLjng d e t h a y t h e c h i s o n o n g d o p r o t e i n n i e u t r o n g m a u n u d c l i e u 2 4 g i d .
D O I T U O N G V A P H U O N G P H A P N G H I D N CLTU 1. Odi tupng nghien citu
Ni?6m benh nhan nghien ciru gom 82 ngudi dUOc clian doan xac dinh viem cdu than man, suy than man (VCTM 29 benh nhan, STM giai doan II. 27 benh nhan, STM giai doan III. 26 benh nhan) da va dang dieulringoai tril, noi tru tai khoa A15-BVTWQD 108,
2. P h u o n g p h a p n g h i e n c y u
S i r d y n g p h U d n g p h a p t i e n c i f u c a t n g a n g . T h u t h a p so lieu t h e o m a u n e n g ,
2.1. Cac chiso nghien cuu
Djnh luong ereatinin, protein nUdc tieu. Chi sd P/C la ty sd dUde may tinh tU ddng tinh, ket qua hien thi ra 1 trong 3 chi sd 150, 300, > 500 sat vdi ket qua cua phep chia protein cho ereatinin nieu trong m i u nUdc tieu ngiu nhien.
2.2. Phutfng phap xu It so lieu S i J d u n g p h a n m e m S P S S 1 6 . 0 d e xLfiy s d l i e u . K E T Q U A N G H I E N CLTU
1 . M o i t i r o n g q u a n g i i i a ch? s d P/C b n U d c t i e u n g i u n h i e n v d i n o n g d p p r o t e i n n i e u 2 4 g i d .
B a n g 1 : M d i t u o n g q u a n giCfa c h i so P/C b nubc t i e u n g i u n h i e n v a p r o t e i n n i e u 2 4 g i d
Pro nifeu P/C 150 300
>500 N
P (tdng n)
<150 10 0 0 10
150-
<300 9 2 0 11
3 0 0 -
<500 0 6 2 8 r=0,913;p<0001:n=29
500-
<1000 0 0 12 12 r = 0 9 0 7 : p < 0 0 0 1 . n = 41
1000- 3500
0 0 27 27
r = 0.66;p<0.001;n = 68
>3500 0 0 14 14
r = 0.49; p < 0.001; n = 82
Y HOC THl/C HANH (843) - SO 10/2012
- He sd tUdng quan giam ddn khi ndng dd protein
nieu tang len. , 2. Mdi tirong quan giiifa ty sd P/C ci nudc tieu
ngau nhien vdi protein ni?u 24gid <t cac nhdm benh.
...^''
^ " ^
" > ^ ^ —
:-2^--::-:-zz
^
PROW S^™^™]
Bllu do 1: Mdl tUdng quan gloa ty sd P/C b mau ni/dc tieu ngau nhlfin vi protein nl&u 24 gift nh6m STM
Bleu d6 2: Min: do tiAmg quan gloa t^ sd P/C d niflJc tllu ngSu nhien vdi protein nieu 24 gift d nhom Viem cau thdn man Cd sy tuong quan rat ehSt ehe giffa protein nidu 24 gid va t j sd P/C trong m i u nude tieu ngiu nhien b ca hai nhdm benh VCTM vd STM vdi he so tUOng quan tuong ifng la"0.985, 0,983; p < 0.001.
3. Moi tuong quan glQa ty so' P/C b mau nudc tieu n g i u nhien vdi protein ni^u 24 gid theo ciz thong so.
3.f. Hing d^ protein ni^u 24 gid:
Bang 2: Mdi tuong quan g\ita ndng d d protein nidu 24 gid vdi t j s d P/C b mau nUdc tieu n g i u nhidn theo nong d d
Protein nieu (mo)
<150 150-
<300 300-
<500 500-
<1000 1000- 3500
srote n
10 11 8 12 27 n
TJsSP/C (mg/g) 163.33 ± 61.76 183.33 ±
74.16 387.5 ±94,18
733,33 ± 238.68 1814.81 ±
858.52
Protein nieu 24li(mg/24h) 1082±31.27 182.18±40.13 380.75 ±78,62 727.92 ±175.56 1894.81 ±859.4
r 0.65 0.82 0.94 0,96 0,96
P
<0,05
<0,05
<0.05
< 0.001
< 0,001
>3500 cong
14 82
5214.29 ± 200,686 1677.44 ±
198,556
6199 ±220,2 1863,57 ± 233.3
0,93 0,985
<o«r
< 0.001 C o s i i tL/dng q u a n r £ t c h a t c h e k h i n 6 n g d a Protein niSu24gld>150mg/ng4y,
- SLT ti/dng quan khii chat che l<hi nong d6 protein nieu 24 gicJ <150 mg/ngay
3.2. MiH: /(JC ciu thin (MLCT):
Bang 3; Moi ti/ong quan gi&a n6ng d6 protein nieu 24 gid v6i t f s6 P/C 6 m i u ni/dc t i l u n g i u nhien theo MLCT
MLCT (mi/pMI)
Tdng
Prolein ni^u 2411 (mg/24h) 2550.24 ± 285,987
1900.4 ± 235,016 1294.8 ± 101.9 1863.57 ±
233.3 TJsSP/C
(mg/g) 2295.4 ± 244,398 1085.19 ±
134,918 1719.17 ±
203,525 1216.67 ±
91.742 1677.44 ±
198,556 0.985 : 0/101 Theo k f t qua bang trSn Ihi MLCT khong M\ h l f i den moi tuong quan giiia ty s 6 P/C d m i u n u S o ' S n g i u nhiSn va protein nifiu 24 g'K). ^ - ^ ^
3.3. G i * ^ *"
Bang 4: M6i tuong quan giQa nong dfl prolein nieu 24 gid vdi ty s 6 P/C 5 m i u nUbc Heu ngau nhien theo gidl
Tdng
Protein ni6u 24gKS TJ stf P/C (m(i/24giO} (mg/g)
2413.51 ±235.62 1539.62±
201.17 2077 78 ±
189,731 16n44±
198.556 0,985 OXtl Theo ket qua bang tr^n thi gidi Knh khong anii hu6ng dSn mdi tuong quan giiJfa 19 so P/C b miu nuflc tieu ngau nhiSn va protein nieu 24 gid.
3.4. Thitkh nuDo tl&i:
Bang 5: M6i tuong quan giite nong d6 protein nieu 24 gid vdi 19 s 6 P/C d m i u nu6c lieu n g i u nhien Itieo the tich nudc t i l u
1000- 2000mi
T}s« P/C (mg/g) 93a,33±86.145 1664.103±204.093 2857.14 ±226,779
Tdng | 8 2 | 1677,44 ±198.556 I " ^ 3 * | 0,9851 <0.I101 Prolan nieu
1879.68 ± 245.71 2954,29 ±
228.2
So luong nudc tieu khong anh huong d i n iiioi tuong quan giQa t9 s 3 P/C d mau nuSc t i l u ngau nliien
va protein nieu 24gid. -^
Y HOC THUC HANH (843) - S6 i ^ B
3 . 5 . T u d i ' :
B a n g 6 ' M d i tUOng q u a n giij'a n o n g d o protein n i e u 2 4 g i d v d i t j s d P/C n i e u b m l i u n u 6 c tieu n g i u n h i e n t h e o t u d i
Nli6m tudi
<29 3 0 - 3 9
4049 50-59 60-69
>70 Tdng
N 7 9 22 19 13 12 82
Protein nieu 24h (mq/24qicr) 1708 5 7 1 14063 2824 64 ±
286 346 2270,64 ± 234 838 2395.95 ± 316,942 1012.08 ±
117102 566.92 ± 53,593 1863 57 ±
233 3 T}s6P/C
(mg/g) 1704 76 ±
1292.78 2664 82 ± 258 535 1924 24 ±
190446 2089,47 ±
268 248 923,08 ± 100,884 633 33 +
61.126 1677,44*
198 556 r
0.99 0.98 0.91 0,99 0 95 099 0 985
P
<0 001
< 0 001
< 0.001
< 0.001
< 0,001
< 0.001
<0001 T h e o ket q u a b a n g t r e n thi m o i t u t i n g q u a n giua ty s d P/C b m i u n u d c tieu n g i u n h i e n v a protein n i e u 24 g i d k h o n g bi a n h h u d n g b d i d o tudi c u a b e n h n h a n ,
B A N L U A N
K e t q u a n g h i e n c y u t h e h i e n r o , khi n o n g d o protein c a n g t a n g thi m d i tUOng q u a n cCia protein n i e u 2 4 g i d v a chJ so P/C c a n g g i a m m a c d i i m o i l U d n g q u a n la t h u a n . D i e u n a y co t h e giai t h i c h la d o chi s d P/C c h i c 6 t h e h i e n kdt q u a c a o n h a t la > 5 0 0 , vi v a y khi n d n g d o protein n i e u 2 4 g i d c a o h d n 1 0 0 0 m g , he thd'ng se k h d n g h i e n thi c h i n h x a c k e t q u a . K e t qua n a y cCia c h u n g tdi c u n g phiJ h d p v d i t a c g i a M o n s h i t a Y va c o n g s U t h y e h i e n n a m 2 0 0 4 .
K d t q u a e h o t h a y m d i _ t u d n g q u a n giij'a protein n i e u 2 4 g i d v a ty s d P/C d m a u nUdc tieu n g i u n h i e n la rat c h a t e h e (r = 0 . 9 8 5 ) v a c o ^ nghTa t h d n g k e p < 0 , 0 0 1 . K e t q u a n a y ci!ia c h i i n g tdi p h i i h o p v6i h a u h e t c a c c d n g trinh n g h i e n ciru k h a c n h u N g u y d n N h u NghTa, S i w a c h S B . . .
C r e a t i n i n dUOc t a o ra b ca. m a k h d i lUdng c d b t h a n h m e n n h i e u h d n ngUdi g i a , t r e c o n v a d a n d n g n h i e u h d n p h u nU. C r e a t i n i n dUdc t h a n loc m a k h d n g tai h a p t h u va bai tiet ra n g o a i nUdc t i e u . C r e a t i n i n n i e u k h d n g bj a n h h u d n g b d i t h e tich v a c h e d p a n . T r o n g khi d d n d n g d o protein n i e u lai p h u t h u p c v a o n d n g d d protein thoat ra v a t h e t i c h nUdc tieu V i v a y c a c y e u t d e d t h e eo a n h h u d n g d e n ty s d P/C d a dUOc c h u n g toi tim h i e u n h u t u d i , g i d i , n d n g d o p r o t e i n v a m y e loc c a u t h a n v a t h e tich n u d e t i e u .
T h e o ket q u a d b a n g 6 t h i m d i tUdng q u a n g i i i a ty s d P/C v a protein 2 4 g i d la rat c h a t c h e v a m d i tUdng q u a n n a y k h d n g b| a n h h u d n g bcii d p tudi ci!ia b e n h n h a n . M a e d u c u n g d b a n g n a y ta c d t h e n h a n t h a y d d p tudi < 2 9 , 5 0 - 5, > 7 0 t u d i c 6 m d i tUdng q u a n tdt h o n s o v d i d o t u d i 4 0 - 4 9 , t u y n h i e n s y k h a c biet n a y k h d n g c d y nghTa t h o n g k e v d i p > 0 . 0 5 . K d t q u a n a y cCia chCtng tdi c u n g p h i i h o p vdi K n s t a l B v a es khi n g h i e n eifu b 5 5 b e n h n h a n b e n h t h a n , C h u N F , F e m g S H v a es n g h i e n eiifu b 4 1 b d n h n h a n t h a n g d m 2 5
n a m v a 16 nu'. T h e o b a n g 4 thi g i i j nrn.
h u d n g d e n m d i tUdng q u a n giu'a proteiri 24 yic P/C d m i u n u d c tieu n g i u n h i e n , m o i l u o n q i|ii.in 2 chi s o d o la m o i t U d n g q u a n t h u a n , rat c h a t cbe v;, ,.
y nghTa t h d n g k e p < 0 . 0 0 1 d ca n a m v a nU V a k e l q u a n a y c i i a c h i i n g tdi c u n g p h i j h d p vdi c a c tac gia K n s t a l B va c s .
N a m 2 0 1 0 , K o s m a d a k i s G v a c s n g h i e n c i f u b 4 5 b e n h n h a n { 2 8 n a m , 17 n u ) b e n h ly t h a n m a n d a dUa ra n h a n xet m d i tUdng q u a n giij'a protein 2 4 g i d va ty so P/C b m a u nUdc tieu n g i u n h i e n k h o n g p i i u tl?u6c v a o M L C T K e t q u a n g h i e n ciru c u a c h i i n g toi thu'c h i e n tren 8 2 b e n h n h a n c 6 M L C T > 5 m l / p i i u t c u n g c h o ket q u a tUdng tU, m o t s d lac g i a k h a c nhu' Chitalia V C , K o t h a n v a c s t h u c h i e n n a m 2 0 0 1 tren 50 b e n h n h a n
Knstal B v a c s nglnien citu a 45 b e n i i n h a n bi b e n h t h a n va dUa ra n h a n xet cho r a n g m o i t ^ d n g q u a n tren CO p h u t h u p c v a o M L C T . X i n G, W a n g M c h o r a n g d o i vdi b e n h n t i a n c 6 M L C T < l O m l / p h i i t Ihi m o i tUdng q u a n giUa p r o t e i n n i e u 24 g\d v a ty so P/C d m a u nUdc tieu n g i u n h i e n se la i<hdng d a n g ke C h u N P va c s n g h i e n cifu d 4 1 b e n h n h a n bi b e n h t h a n va du'a ra n h a n xet M L C T > 7 0 m l / p h u t thi c 6 m o i tUOng q u a n tot h d n s o vdi M L C T < 7 0 m l / p h i i t .
C h i i n g tdi thiet nghT, khi k h a o sat vdi c d m a u Idn i?dn thi ket q u a se rd r a n g h d n , V a vi v a y , d e c6 t h e ket luan c h a c c h a n v e v a n d e n a y thi c a n p h a i c o m o t n g h i e n cifu vdi qui m d Idn h d n .
Ket q u a n g h i e n c i f u t h a y giu'a protein 24 g i d va ty so P/C b m i u nUdc t i e u n g i u n h i e n co m d i l U d n g q u a n kha c h a t c h e khi n d n g d p p r o t e i n < 1 5 0 m g / n g a y , 2 chi s d tren c d m d i tUdng q u a n rat c h a t c h e h d n khi n o n g d o protein > 1 5 0 m g / n g a y S u k h a c biet n a y la c 6 y nghTa thd'ng ke vdi p < 0 . 0 0 1 , O i e u n a y c 6 nghTa la m d l t u o n g q u a n vdi p r o t e i n n i e u 2 4 g i d va ty s d P/C b m a u n u d c tieu n g a u n h i e n c 6 p h u t h u d c v a o n d n g d p protein C h i i n g tdi n h a n t h a y , m d i t U d n g q u a n g i y a 2 chi s d tren s e tot hOn khi n d n g d d protein nieu n a m t r o n g k h o a n g 3 0 0 - 3 5 0 0 m g / n g a y , s o n g s y k h a c biet d o chUa CO nghTa t h d n g k e vdi p > 0 0 5 .
K d t q u a cOa c h u n g tdi c u n g p h i j h d p vdi c a c n g h i e n cifu c u a c a c tac gia T o m g S, C R i g a t t o n g h i e n ciHi tren 2 8 9 b e n h n h a n g h e p t h a n c h o r a n g khi protein nieu tren 3 g / n g a y thi d p c h i n h x a c g i a m , C h u N.F. d u a ra n h a n xet protein n i e u > 1 g / n g a y se c h o md'i tUdng q u a n tdt hOn s o vdi n d n g d p protein <1 g / n g a y , n g o a i ra c d n eo m o t s d t a c g i a k h a c . S o n g , ket q u a c i j a c h i i n g toi lai k h a c v d i c a c tac g i a K o s m a d a k i s , K n s t a l B. thi m d i t u o n g q u a n giCfa protein n i e u 2 4 g i d v a ty s d P/C d m a u n u d c tieu n g i u n h i e n k h d n g p h u t h u p c v a o n d n g d p p r o t e i n .
Ivldi t u o n g q u a n giij'a p r o t e i n n i e u 2 4 g i d v a ty s d P/C b m i u n u d c t i e u n g a u n h i e n la rat c h a t c h e v a m d l t U d n g q u a n n a y k h o n g p h u t h u o c v a o t h e tieh n u d e t i e u . D i e u n a y c u n g p h u h d p v d i ly t h u y e t d a noi b p h a n t r e n .
K o o p m a n M G , R T K r e d i e t t i e n h a n h c y 3 t i e n g lay n u d c tieu m d t l a n , b a t d a u t y 6 g i d s a n g v a e h o n h a n xet m i u n u d c t i e u lay t r o n g k h o a n g 6-9 g i d la e h o m d i t u o n g q u a n tdt n h a t . K o s m a d a k i s G v a e s , Kristal B v a
Y HOC TH0C H A N H (843) - S6 10/2012
cs ddu Idy m i u nghidn citu vio 3 thdi didm 8 gid, 12 gid, 14 gid va dua ra nhan xet m i u nudc tidu Idy vao budi sang va budi t r i ^ ed mdi tUOng quan tdt hOn so vdi m§u budi chidu.
Do mau cDa chiing tdi chi Idy vao budi sang eho phi\ hpp vdi kham b&nh cCia bdnh nhdn ndn chung tdi chua ed nghidn cifu vd vdn dd nay
KE'T LUAN
Nghidn cyu 82 bdnh nhan VCTM vd STM giai doan 2, 3 tai khoa A15 bdnh vidn Trung Udng Qudn ddi 108.
chiing tdi njt ra mdt sd kdt ludn sau:
- Ty sd P/C b m i u nude tidu ngiu nhidn md'i tuong quan thuan rdt chat che vdi ndng dd protein nidu 24 gid. (r = 6.985, p < 0.001). Mdi tuong quan giCte 2 chi sd ndy khdng phu thude vao tudi, gidi, thd tieh nude tidu.
- Mdi tuong quan giCfa protein nidu 24 gid va tf sd P/C d m i u nude tieu ngiu nhidn khdng phu thudc vao MLCT, nhung ed phu thude vdo ndng dd protein nidu.
TAI LlfeU THAM K H A O
1. Trdn Van Chdt (2008) "Thdm dd mUc IQC ciu th$n trong thi/ hinh lim sing", Bdnh than. NXB Y hpc, tr.72 - 78.
2. NguyIn Thj Thjnh. Trdn Van Chdt (1997). -Vnh hinh bdnh thin tiit ni$u diiu trj ndi tnj t^l khoa Th$n - Tiit ni$u Bdnh Viin Bach Mai tit 1991 - 1995", Cdng trinh nghien cdu ktioa hoc 1995 - 1996, Benh vidn Bach Mai.
tr. 181 - 186.
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NGHIEN COU VA THIET KE VECTOR BIEU HIEN PROTEIN El CUA VIRUS CHIKUNGUNYA TREN Ht E.COLI
v u X U A N NGHlA
Hgc vi^n Quin y, D^ hqc tdng hpp Wuenburg-CHLB Btk
TOM T X T
Virus Chikungunya (CHIKV) giy ra cic vy dich sit Chikungunya d Chiu Phi, in Dd, Ddng Nam Chiu i vi gin day la khu vuc Nam Chiu Au. Biiu hi^n diin hinh khi nhi§m CHIKV li sit, phit ban vi vidm da khdp. Di phit triin ky thuit chin doin huyit thanh die hi$u CHIKV, vector biiu hidn protein tii ti hrfp BI cOa virus duac thiit ki vi bi^u hiin trin hi E.coli.
SDS-PAGE vi Western blot phin tich cho thiy, protein tii ti hpp El cOa CHIKV biiu hidn tit trdn h^ E.coli vi cd tn?ng lupng phin tCtIi 42 Kd.
SUMMARY
Chikungunya virus (CHIKV) causes epidemic outbreaks of CHIK fever in Africa. India, South-East Asia, and recently Southern Europe. CHIKV fever is characterized by high fever, rash and pc^yarthralgia.
To develop a CHIKV-specific serological diagnostic test. El recombinant protein expressed vector was designed and expressed on the E.coli system. SDS- PAGE and Western blot were applied and analyzed to show that the recombinant protein was expressed and the molecular weights of structural El proteins was 44 Kd.
Vinjs Chikungunya (CHIKV) Id nguydn nhan gSy bdnh sdt CHIK d ngudi vdi cic tridu chiJitig diln hinh nliu sdt, dau khdp, vidm khdp va phat ban. CHIKV Id mdt atphavims thudc hp Togaviridae va dUdc ISy truydn thdng qua vector truydn t)dnh Ae. aegypti, Ae- albopictus. Genome cilia CHIKV la s d RNA ddn c6 kich thudc khoang 12kb chifa gen ma hoa protein cdu tmc la protein E l , protein vd E1, E2.
Hidn nay. chdn dodn huydt thanh hoc CHIKV si/
dung todn bd khang nguydn cOa vims nhd ELISA, m i l n dich hujnh quang. Bdi vdy, trong nghidn cdu ndy chung tdi Cfng dung cdng nghe sinh hoc phaii tif thidt kd vector bieu hien protein tdi td hop dSc hi&u, protein E l . djnh huGng ijfng dung trong chan doan huydt thanh hpc.
V A T L i f U v A PHl/ONG P H A P NGHlfiN COU ^ 1. T90 vector bieu hi^n CHIKV protein El t i i to h^p.
CHIKV-Wue1 duoc nudi cdy va phan lap tif td bao mu6i C6/36. To&n bd Genome cOa CHIKV diwc tach
Y HOC THVC H A N H (843) - S6 10/2012
