KHOA HOC CdNG N G H |
yixiGHIEIV ClJU T H A I X I H PHAIM L O A l VI KHUAIM TREIV TU H A I {LUTRARtA PHiUPPilHARUM n^lEVE., 1 B 5 4 )
MUOI TAI VIET IVAM
Truong Thi My HanhS D i n g T h i Lya^ v i Phan Thi van' T6MTAT
Nghign cuu diroc ti^n h i n h tai vinh Ha Long va vinh Cam Ranh, thai gian t h u c hi^n tir t h i n g 1/2013 d^n t h i n g 12/2013. Tu hai c6 bieu hien sung voi va k h o n g co bieu hien s u n g voi duge t h u m i u d e phan tich nham xac dinh t h i n h phan loai vi khuan c 6 k h a nang gay b&nh. Nghi6n ciiu da s u d u n g phuong p h i p nuoi c^y vi khuan tr^n cac moi tnrong nuoi c^y chon Ipc va E t API 20E d l dinh d a n h loai vi khuan. K^t qua nghien ciiu cho t h i y tu hai bj b e n h sung voi 6 giai doan giong n h i l m 7 loai, a giai doan t h u a n g pham nhiim 11 loai; tu hai k h 6 n g b i l u hi^n b e n h sung voi a giai doan giong n h i l m 11 loai vi k h u i n , a giai doan thmmg pham n h i l m 6 Ioai. Nghien ciiu c u n g da xac dinh s u hien di6n dong th6i c a 3 loii vi k h u a n ( V. alginolyticus, V. choleraevz V. splendidus) tren tu hai a c i 2 vimg nu6i vinh Ha Long v i vinh Cam Ranh. K i t q u i nghidn ciiu la c a so khoa hoc giiip dinh h u o n g nghidn ciiu tac nhan gay b e n h tu hai s u n g voi a Vi^t Nam.
Tiikh6a: Vi khudn, sung vdi, tuhai.
I.MffBAU
Tu hii {Lutraria philippinarum Reeve, 1854) l i l o i i t h u y s i n n u o i c o g i i t r i c a o v^ d i n h d u o n g l i n k i n h t^. N g h 6 n u o i t u h i i o V i ^ t N a m p h i t t r i e n m a r d i t r o n g n h i m g n a m g a n d i y , d i e b i ^ t l a n a m 2010 v a 2011. N a m 2 0 1 1 , 6 V i n D o n , Q u a n g N i n h c 6 t o i 6 0 t n 6 u c o n g i o n g d u p e t h a n u o i , c a o g a p 3 l i n s o v o i n a m 2010 ( B i o c a o ciia U B N D h u y e n V i n D o n , 2012). N a m 2010 a C i t B i , H a i P h o n g c o t o i 226 b ^ v i 3.000 g i i n n u o i t h a s o voi 2 0 ht t h i n u o i n i m 2 0 0 1 ( B i o c a o c u a h u y e n C i t B i , H i i P h o n g , 2012). T u y n h i e n , d e n n a m 2013 di$n tich n u 6 i b i t h u h e p d d n g thoi h i n h thiic n u o i c u n g d o n di6u h o n ( t a p t r u n g chii y e u n u o i l o n g ) . O Hai P h o n g t r o n g k h u vuc L a n H a n a m 2011 c 6 236 b 6 v i 100% sd hh n i y t u h i i c o h i e n t u o n g c h e t voi b i l u h i e n s u n g voi. H i $ n t u o n g t u h i i c h e t x u i t h i ^ n o c a tu h i i g i o n g b e (2 m m ) , t u h i i g i o n g Ion (1-3 c m ) v i t u h i i k i e h c o t h u o n g p h a m .
N g a y s a u k h i d o t d i c h d a u tien x i y r a v i o c u o i n a m 2011 B o N o n g n g h i e p v a P h a t t r i e n N o n g t h o n d a chi d a o c h o Vi6n N g h i d n c u u N u o i t r o n g T h i i y s a n 1 t h u c h i e n d^ tai " N g h i d n c u u d i c h bfenh g a y c h ^ t h i n g loat 6 tu h a i {Lutraria philippinarum R e e v e , 1854) n u o i t a i Viet N a m " , v o i m u c ti6u x a c d i n h r o n g u y e n n h a n , t i c n h i n g i y r a h i e n t u o n g t u h a i s u n g voi c h e t h a n g loat. N g h i e n ciiu t h i n h p h i n loai vi k h u a n t r e n t u h i i b e n h s u n g voi l i m g t n o i d u n g t h u c h i ^ n t r o n g k h u o n k h o d^ t i i . D 6 n g t h a i d a y l i b a o c a o d a u ti^n c h i r a s o l o i i vi k h u a n nhifim t r 6 n tu h a i n u o i tai Viet N a m , b u o e d a u t a o c o s o k h o a h g c c h o
' Vi^n Nghien cim Nuoi trong Thiiy san 1
v i e c d i n h h u o n g n g h i e n c i i u x i e d i n h t i c nhan gay b g n h s u n g v 6 i t r e n t u h a i , t u d o d u a r a bi^n phap p h o n g v i tri bfenh h i ^ u q u a .
2. PHUDNG PHAP NGHBU Ciiu
2.1. ThW gian, v i t Ufeu v i dia di^m nghi6n ciiu Nghien cuu dugc thuc hi^n tu thing 1/2013 d&
t h i n g 12/2013, dia diem thu m i u nghidn cim la viing nuoi trong dilm thudc vinh Ha Long (Quang Ninh vi Hai Phong) v i vinh Cam Ranh (Cam Phiic Bic - Cam R a n h - K h i n h H6a).
Tu h i i nuoi b giai doan gidng v i thuong ph^m voi bilu hi§n b6nh sung voi v i kh6ng c6 bilu hi^n bSnh dugc thu m i u p h i n tich. T6ng sd 296 mSu tu h i i (bao gom 176 m i u tu hai voi bilu hi6n sung v6i v i 120 mau tu h i i khong bilu hifen sung vol) duoc thu v i p h i n tich tai phong thi nghidm thu6c Trung t i m Nghifen cuu Quan trac Canh bao M6i truong vi Phong ngua dich b$nh khu vuc miln Bic (CEDM^, Vien Nghien cuu Nuoi trong Thiiy san I (RIAl).
2.2. Phuong p h i p p h i n l i p vi k h u ^ Phuong phip nu6i ciy, p h i n lip vi k h u i i dua tr^n phuong phap nghidn cuu b^nh vi khuan trfin dgng vat tiiuy sin cua Frerichs v i Millar (1983, 1993). Moi tiiiong Nutiient broth agar v i TCBS du^
su dung de nuoi cay vi khuan, d i y phan ling sinh h6a duoe tien hanh theo test API 20E v i dinh danh trfin apiweb.
8. KET QUA WHEU CUU Vit I H A D LUAN
3.1. D i u hi^u b^nh ly cua b^nh simg v6i trftn tu h i i v i ty 16 nhifem vi khu5n ciia mSu thu phan tich
Nghidn ciiu theo doi trong c i c dgt dich b6nh gfty c h i t hang loat trSn tu h i i nu6i tai Vinh H? Longvi
90 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/20U
KHOA HOC C 6 M G N O H I
vinh Cam Ranh cho fli^y, tu hii benh ch^t cd cimg chung mot dau h i ^ b^nh ly, ggi chung l i benh sung v6i. Tu hii sung voi co dau hieu b^nh ly trai qua 4
giai doan (1) voi sung; 2) xung quanh voi duge bao bgc boi ming tring nuoc; 3) voi hi sin len v i lop bieu md voi hi bong trdc; vi 4) voi hi teo (Hinh 1).
Hinh 1. Bilu hi^n b^nh 1^ giai doan 1 d i n giai doan 4 tiiuongphlm;Bl,B2, Kit qua phin tich ty 16 nhi^m vi khuan trdn tu hai hi benh sung voi v i tu hii khdng cd bilu hien sung vdi cho thay, nhin chung ty 16 nhilm vi khuan trfen tu hii nudi cao (44%-88%) v i ly le nhilm vi khuan tr6n tu hii thu tai vinh Cam Ranh cao hon d vjnh Ha Long. O vinh Ha Long, tu hii b giai doan gidng vi thuong phim khdng co bilu hien bdnh nhilm vi khuan cao hon tu hii benh vdi ty le nhilm tuong ling tren tu hai khong cd bilu hien benh tu 5196 din 53% va ti-dn tii hii b^nh sung vdi tir 43% den 49%. Trong khi dd k i t qua nghidn ciiu ciia miu tu hai thu tai vinh Cam Ranh lai cho k i t qua nguoc lai. Tu hai d giai doan gidng v i thuong phim bi benh sung vdi nhilm vi khuan cao hon tu hii khdng bilu hidn b^nh vdi ty 1^ nhilm tuong ling trln tu hai benh tir 65% din 88% va ti-dn hi h i i khdng bilu hien b'^nh tit 50% din 70% (Hinh 2).
ciia bdnh sung vdi trln tu hii (Al,A2,A3viA4Tuhii B3,viB4 Tuhai giong)
3.2. Kit qua phan tich vi khuan trfin tu hii a giai doan gidng
Bang 1. Ty 1^ % vi khuin nhilm tr&i tu hii b#nh sung vdi v i tu hai khong c6 bilu hidn b^nh a giai doan
gi6ng thu tju vjnh Cam Ranh vi Vjnh Ha Long
•GiOLia •Tlniaiiii|ilii«
bbii
SIT
1 2 3 4 5 6 7 8 9 10 11 12
Thanh p h ^ lo^ vi khuan V.wytilii V. splendidus Staphylococcus sp
V. barveyi V. cholerae V. tubiashii V. aigmoiydcus V.Huvialis V. parahaemolydcus Pseudomonas sp
V. neptunius V. mediterrane
Tv le nhiSm khuan («) Tu hai khong c6
bi^u hifen b^nh sungvbi
1.8 10,9 2,3 7,8 10,2 3,9 16,4 1,6 0,8 0,8 0,8 0
Tuhii simg v6i
0 0 0,6 7,2 0,6 0 13,8
0 6,6 2,6 0 9,2
TuhkKrngMi . Tuhtllilitngbilj Tuntinmgvd
Hinh 2. T^ 1$ % mlu bl hii sung vdi vi hi hii khdog c6 bi&t hi$n b^nh dgiai d o ^ gidng vi tiiuongph^ nudi
tfli Vjnh Cam Ranh vi Vjnh H? Long nhilm vi khu&i
Ghi chii: V. Vibrio
Kit qua bang 1 cho thay b giai doan giong, su bgi nhilm cua nhilu loii vi khuan khic nhau ldn tu hii khdng bilu hien bdnh cao (11 loii), trong khi dd tu hii benh sung vdi chi nhidm 7 loii vi khuin. Trong so loii VI khuan phin tich cd 6 loai bdt gip trdn ca tu hii khong bilu hidn bdnh vi b$nh sung vdi, tuy nhidn vdi ty 1? thap, cao nhat li loii V. alginolyticus (16,4% trdn tu hii khdng bilu hi^n b^nh vi 13,8% trdn tu hai bdnh sung vdi) (Bang 1). Ty 1| nhilm vi khuin thap nhat (0,8%) doi voi 3 loii vi khuin V.
91
KHOA HOC CdHG NGHfi
parahaemolyticus, V. neptunius v i Pseudomonas sp tren tu hai khdng bieu hien benh, 0,6% doi vdi vi khuin Staphylococcus sp. v i V cholerae tren tu hii bdnh sung vdi.
Cung gi6ng nhu mdt sd loai ddng vit thiiy sin khac, loii nhuyin till 2 manh vo o cac giai doan phit triln khac nhau tiiudng cam nhidm vdi loii vi khuin khac nhau. Hidn nay nghien ciiu benh vi khuan trdn nhuyin till 2 manh vo dugc chia lim 3 giai doan tuong ling voi 3 giai doan phat triln ciia vit chu (giai doan au trung, giong vi truong thinh). O giai doan gidng, nhuyin t h i tiiudng nhilm 4 benh chu ylu, ph6 biln sau: "Juvenile Oyster Disease QOD) do alpha-Proteobacteria" (Bricelj et al., 1992; Boettcher et al., 1999, 2000), bdnh in mon bin l l mat lung (do Cytophaga sp-like) (Dungan and Elston, 1988;
Dungan et al., 1989), bdnh hdi chung ip xe do vi khuin gram duong (Elston et al., 1999) va gan diy xuat hidn benh "summer mortalities" do V.
spendidus (Lacoste et al., 2001; Waechter et al, 2002). Trong nghien ciiu nay 2 loii vi khuan V.
splendidus vi vi khuan gram duong Staphylococcus sp da xac dinh cd mat tren tu hii khdng c6 bilu hien bdnh, tuy nhidn voi ty Id thap lin lugt tuong ung 10,9% va 0,6%.
3.3. Kit qui phin tich vi khuin trdn tu hii 6 giai doan thuong p h ^
Bing 2. Ty I^ % vi khuin nhilm trdn tu hii bdnh sung vdi v i tu hii khdng cd bilu hi^n b^nh b giai doan
Kit qua phan tich vi khuan ti-|n tu hii giai do^n tiiuong pham trai ngugc vdi k i t qua phin tich ri khuin b giai doan gi^ng. Cu t h i tu hai hi bdnh simg vdi bat gap 11 loai vi khuan, trong khi dd trdn tu hai khdng CO bilu hidn bdnh chi b i t gap 6 loai. Tu hai benh sung vdi bat gap V. cholerae vai ^ l l nhilm cao nhat 21,7%, diy cdng l i loii b i t gap thip nhit trln tu hai khdng bilu hidn benh (1,7%). Ddi vdi tu hai khong bilu hidn bdnh V. mytilii nhihm voi ty ll cao nhit (27,1%) nhung l i 0% tren tu hii bdnh sung voi teo voi (Bang 2).
Q giai doan trudng tiiinh cua nhuydn t h i thuong bat gap 5 bdnh vi khuan bao gom: "Brown Ring Disease" (do V. tapetis) (Paillard, 2004), b&ih
"summer mortahties" (do V. splendidu^ (Waechter et al., 2002; Le Roux et al., 2002), benh OOD) do Roseovarius crassostreae (alpha - Proteobacteria) (Ford and Borrero, 2001), bdnh Nocardiosis do vi khuin Norcardia (Friedman et al., 1991) va bdnh do Ricketssia. Trong nghien ciJru nay, trong so 11 lo^vi khuin phan lip dugc c6 2 loii V. tapetis va K splendidus dugc bilt d i n l i tic nhin chinh gay bdnh vong nhin niu v i benh chet miia hd trdn nhuydn th^, tuy nhidn ty Id nhidm thip lan lugt tuong ling 1,396 vi 4,5%, dong thdi bilu hidn bdnh ly khdng gidng nhu mo ta tir cac tic gii.
3.4. Tiiinh p h ^ loii vi khuin nhilm trdn hi h^
nudi b 2 vimg nudi k h i c nhau
Bing 3. So sinh ty 1^ % vi khuin nhilm ti-dn tu h4i hooE
STT
1 2 3 4 5 6 7 8 9 10 11 12
Iff pham thu tai vinh C Thanh phan loai vi
khuan V. tapetis V. cholerae V. splendidus
V. tubiashii V. ordalii V. harveyi V. vulniScus V. parahaemolyticus
V. alginolyticus V. Huvialis V. mediterrane V. mytilii
am Ranh vi vjnh Ha Long Ty le nhiem khuan (%) T u h J i khang
CO bieu hien benh sung voi
0 1,7 5,1 0 0 1,7 0 1,7 8,5 0 0 27,1
Tuhai sung voi 1,3 21,7
4,5 1,9 5,1 7,6 7,6 2,5 13,4 2,5 19,1
0 bt^i STT
1 2 3 4 5 6 7 8 9 10 11 12
ih sung v6i or vinh C Thanh phan loai vi
khuan V. tapetis V. cholerae V. splendidus V. tubiashii V. ordalii V. harveyi V. vulniScus V. parahaemolyticus V. alginolyticus Pseudomonas sp V. mediterrane Staphylococcus sp
un Ranh va vin iHaLoiK Ty le (%) vi khuan nhiSm Irtn
tuh^smiffvdi Vinh Cam Ranh
1,7 29,6
3,5 2,6 6,9 10,4 10,4 6,9 20,8
3,5 0 0
Vinh Ha Long 0 2,6 3,9 0 0 0 0 0 13,2
0 57,8
1,3 _ -
Ghi chu. V: Vibrio
Ghi chu. V: Vibrio
Tinh da dang thanh phin loii vi khuan n h i ^ trdn tu hai bdnh t h i hidn trdn m l u thu tai vinh Cam Ranh (10 loii), trong khi dd b vinh Ha Long chi 5 loi 92 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/2014
KHOA HOC C 6 H G N G H £
(Bang 3). 6 vinh Cam Ranh loii d i i l m ty Id cao nhit V. cholerae (29,6%), tilp din V. alginolyticus (20,8%) vi 8 loai cdn lai chilm ty Id < 11%. O irinh Ha Long, dudng nhu cd su t i p tiimg v l thanh phan loii, V.
mediterrane nhilm voi ty Id cao nhat (57,8%), tilp din V. alginolyticus (13,2%) v i 3 loii vi khuin cdn lai nhidm vdi ty Id tiiip (< 5%) (Bang 3).
Ddi vdi miu tu hai khdng co bilu hidn bdnh, thanh phan loii vi khuin nhidm tren mlu thu tai vinh Ha Long cd tinh da dang v l thinh phan loii 9 floii), trong dd V. mytilii nhilm vdi ty Id cao nhit (37%), tilp din V. splendidus (11%), sd loii cdn lai nhilm vdi ty Id tii^p (<10%) (Bang 4). 6 vinh Cam Ranh thu dugc 3 loii bao g6m V. alginolyticus, V. Suvialis v i Pseudomonas sp vdi ty Id nhilm lan lugt tuong ling 37,5%, 6,3% va 3,1% (Bang 4). Trong sd dd duy chi cd Valginolyticus xuit hidn trdn m i u thu d ca 2 vimg nudi vdi ty Id nhilm 37>5% (vinh Cam Ranh) v i 7%
(vinh Ha Long) (Bang 4).
Vi khuin thudc nhom Vibrio, Staphylococcus v i Pseudomonas li nhimg nhdm vi khuan cd mat ph6 biln trong mdi tnrdng nude min, trdn cic ddi tugng nudi ddng tiidi chung c6 k h i nang giy bdnh. V.
alginolyticus giy bdnh xuat huylt lo lolt trdn c i Chem nudi dr An DO (Sharm et al., 2011), V. barveyi, V.
parahaemolyticus, V. alginolyticus, V. anguUlarum. V.
vulnidcus, vi V. splendidus li cic loii hdn quan din bdnh ti-dn tdm nudi (Shruti et al., 2012) v i V.
paraheamolyticus, V. alginolyticus, V. harveyi. V.
cholerae, V. vulnideus. Pseudomonas sp nhidm trdn ci Song, Gid nudi tai Vidt Nam (Vin et al., 2006).
Bing 4. So sinh ty 1^ % vi khuin nhilm trln tu hii khdog bilu hi|n b | n h simg vdi 6 vinh Cam Ranh v i
vjnh Hg Long STT
1 2 3 4 5 6 7 8 9 10 11
Thinh phin lo^ vi khu£n V.mytili V. splendidus V. harveyi V. cholerae V. tubiashii V, alginolyticus V. parahaemolyticus V. neptunius Staphylococcus sp Psedomonas sp
V.Suvialis
Ty 1& 00 vi khu£n nhilm trgn tu hai kh6nR b i ^ hi6n benh sung v6i Vinh Ha Long
37 11 7 9 4 7 0,6 0,6
1 _^
0 0
Vinh Cam Ranh 0 0 0 0 0 37,5
0 0 0 3.1 6,3 Ghi chd. V: Vibrio
4.K^U]AN
Tu h i i bdnh sung vdi eiing nhu tu hii khdng bieu hi^n bdnh cd ^ Id nhidm vi khuan cao dao d^ng 44% din 88%.
Tu hii benh sung vdi nhilm 11 loai vi khuan vdi ty Id nhilm thap, trong dd V. alginolyticus ntuhn CBO nhat trdn giai doan gidng (13,8%) v i V. cholerae nhilm cao nhit b giai doan thuong pham (21,750 -
Tu h i i khong bilu hidn bdnh nhilm 11 loai vi khuin, trong dd V. alginolyticus nhidm voi ty 1^ cao nhit trdn giai doan gidng (16,4%) v i V. mytilii xHtakm.
cao nhat b giai doan thuong pham (27,1%).
TTiinh phan loii vi khuin nhilm trdn tu hii bdnh sung vdi nudi 6 2 vdng (vinh Ha Long v i Vinh Cam Ranh) tuong ddi Idiic nhau. Trong sd 10 loii phin lip dupe duy chi cd 3 loii xuit hidn ddng thdi trdn c i 2 viing nudi: V. alginolyticus (13,2%-28,0%), V.
splendidus (3,5%-3,9%) v i V. cholerae (2,6%-29,e%).
TJU L i u THAM KHAO
1. Bio cio ciia UBND huydn Vin Ddn ngiy 6/7 nam 2012 v l tinh hinh dich bdnh tu hii nuoi tai Vin Ddn, Quang Ninh.
2. Bao cao cda huydn Cit B i giii Tdng cue Thiiy san nim 2012 v l tinh hinh khio s i t dich bdnh tu hii nudi tai Cit Bi, Hii Phdng.
3. Ld Vin Yin v i ctv (2009). Bao cio tdng k i t d l tii "Nghidn ciiu mpt sd bdnh thudng gap trdn dc huong (Babylonia areolata) gidng v i nudi thuong pham tai cic linh miln Trung, cic bi^n phap phdng tii".
4. Phan Thi Vin v i ctv (2006). Bio cio tdng k i t d l tii "Nghidn cihi tic nhin giy bdnh phd biln ddi voi c i Mu, c i Gid v i d l xuit cic giii phip phdng h i bdnh "-
5. Boettcher K J., Barber B. J., Singer J. T., 1999. Use of antibacterial agents to elucidate the etiology of Juvenile Oyster Disease (IOD) in Crassostiea virginica and numerical dominance of an a-proteobacterium in JOD-affected animals. Appl.
Environ. Microbiol. 65,2534-2539.
6. Boeticher K. J., Barber B. J., Singer J. T., 2000. Additional evidence that juvenile oyster disease is caused by a member of the roseobacter group and colonization of nonaffected animals by Stappia stellulata-Iike strains. Appl. Environ. Microbiol. 66, 3924-3930.
7. Bricelj V. M., Ford S. E., Borrero F. J., Perkins F. 0., Rivara G., HiUman R. E., Elston R. A., Chang J.,
NdNG NGHlfP VA PHAT TRI^N N 6 N G T H 6 N - KY 2 - THANG 9/2014 93
KHOA HOC CdNG NGHi 1992. Unexplained mortahties of hatehery-reared,
juvenile oysters, Crassosirea virginica (Gmelin). J.
Shellfish Res. 11,331-347.
8. Dungan C. F., Elston R. A., 1988.
Histopathological and ultrastructural characteristics of bacterial destruction of hinge Ugaments of cultured juvenile Pacific oyste.T%,Crassostrea gigas.
Aquaculture 72,1-14.
9. Dungan C. F., Elston R. A. M. H. S., 1989.
Evidence and colonization and destruction of hinge ligaments in cultured juvenile oysters {Crassostiea gigas) by cytophaga-like bacteria, ^ p l . Environ.
Microbiol. 55,1128-1135.
10. Ford S. E., Borrero F. J., 2001. Epizootiology and pathology of Juvenile Oyster Disease in the Eastern Oyster, Crassostiea virginica. J. Invertebr.
Patiiol. 78,141-154.
11. Frerichs, G. N. and S. D. Millar, 1993.
Mannual for the isolation and indentification of fish bacterial pathogens. Institute of Aquaculture, University of Stirling, Scotiand. 60 pp.
12. Friedman C. S., Hedrick R. P., 1991b. Pacific oyster nocardiosis: Isolation of the bacterium and industion of laboratory infections. J. Invertebr.
Pathol. 57, 109-120 gigasmortality events. Aquat.
Living Resour. 15,251-258.
13. Gamier, M., Y. Labreuche, C. Garcia, M.
Robert and J.-L. Nicolas, 2007. Evidence for the involvement of pathogenic bacteria in summer
mortahties of the Pacific oyster Crassostrea giga&
Microbial Ecology 53:187-196.
14. Le Roux F., Gay M., Lambert C , Waechter M., Poubalanne S., Chollet B., Nicolas J. L., Berthe F., 2002, Comparative analysis of Vibrio splendidus- related strains isolated during Crassostrea.
15. Le Roux, F., M. Gay, C. Lambert, J. L Nicolas, M. Gouy and F. Berthe, 2004. Phylogenetic study and identification of Vibrio spIendidus-rtXiAsA.
strains based on gyrB gene sequences. Diseases of Aquatic Organisms 58; 143-150.
16. Paillard, C , 2004. A short review of brown ring disease, a vibriosis affecting clams, Ruditapes philippinarium and Ruditapes decussates. Aquat living Resour. 17:467-475.
17. Shruti Chatterjee and Soumya Haldar, 2012.
Vibrio Related Diseases in Aquaculture and Development of Rapid and Accurate Identification Methods.
18. Sulumane Ramachandra Krupesha Sharma, Gaurav Rathore, Dev K. Verma, Narasimhulu Sadhuland Kuttickal Kuriakose Philipose, 2011.
Vibrio algmolyticus'udectxon m Asian seabass {bates calcarifer, Bloch) reared in open sea floating cages in India.
19. Waechter M., Le Roux F., Nicolas J, L, Marissal E., Berthe F., 2002. Characterisation of Crassostrea gigasspat pathogenic bacteria. C. R.
Acad. Sci. Paris 325, 231-238.
A STUDY ON BACTERIA SPECIES IN CULTURED OTTER CLAMS (.LUTRARIA PHILIPPINARUM^EEm, 1854) IN VIETNAM
Truong Thi My HanhS Dang TTii Lua^ and Phan Thi Van'
^ Research Institiite for Aquaculture No. 1 Summary
The research was conducted in Ha Long Bay and Cam Ranh Bay, Vietnam from January 2013 to December 2013. Otter clams with and without swollen siphon signs were collected for identification of bacterial species.
Bacterial selection media and API 20E tests were used to isolate bacteria species in tiie study. The result showed tiiere were 7 and 11 bacterial species isolated from otter clam wifli swollen siphon at tiie fingerling and commercial stages, respectively. While tiiere were U and 6 bacterial species from otter clam without swollen siphon at ttie fingerbng and commercial stages, respectively. However, tiiis study also found 3 bacterial species, including V. alginolyticus, V. cholerae snd V. splendidus in botti otter clam cultured in Ha Long Bay and Cam Ranh Bay. The findings served as baseUne information for futiier identification of pathogens on swollen siphon disease in otter clam m Viet Nam.
Key words: Bacteria, swollen siphon, otter clam.
Ngudi phin bi|n: TS. Biii Quang T l Ngiy nhin bii: 28/7/2014 Ngiy tiidng qua phin bi|n: 29/8/2014 Ngiy duylt ding: 5/9/2014
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/2014
