NGHIEN CU'U DA DANG DI TRUYEN VA DANH GIA TINH DOC CUA CAC CHUNG NAM GAY BENH DAD ON
CYVONG NAM TRUNG BO
Study on Genetic Diversity and Virulence Evaluation of Blast Rice (Pyricularia grisea) in The South Central Region
Nguyen Thi Thu Thuy. va Tru,ceng Thi Wing Hai Khoa NOng hoc, truerng Doi hoc NOng lam Hue
Ngay nhan bai: 24.9.2015 Ngay c170 nhOn: 10.11.2015 Abstract
Genetic diversity and genetic relationships among 18 Pyricularia grisea isolates collected from South Central of Vietnam was revealed by using 12 RAPD markers. Based on genetic distance, 18 P. grisea isolates were divided into 4 major groups. While group A included four P. grisea isolates, which had genetic similarity ranged from 0.58 to 0.77; group B included four P. grisea isolates having a range of genetic similarity coefficient from 0.58 to 0.76 and divided into two minor groups; group C included eight P. grisea isolates, which had genetic similarity ranged from 0.63 to 0.74 and divided into three minor groups, group D included two P. grisea isolates having a genetic similarity coefficient of 0.74. Total of 9 isolates representing the four genetic groups and different locations were selected to evaluate their virule'nce. The results showed that isolate QN4, which was collected from Que Son, Quang Nam, had highest virulence and following was isolate QNG2, which was collected from Son Tinh, Quang Ngai. The lowest virulence was observed in isolates BD2, which was collected from Phu Cat, Binh Dinh. The information found in this study may be usefull in rice breeding program for blast resistance in South Central region.
Keywords: Blast rice, virulence, rice, Pyricularia grisea, South Central.
1. DAT VAN DE
Benh dao On 6' 10a do nam Pyricularia grisea gay ra, la mot trong nhirng nguyen nhan lam giam nang suet 10a gao nghiem trong nh5t a Viet Nam cung nhu' tren the giai. Khi cay lua bi nhiern benh, tat ce cac mO la co the bi nam tan cong, d5c biet la khi benh gay hai tren bOng co the dan den mat nang suet hoan toan. San luang lua bi thiet hai do benh dao on gay ra khoang 10 - 30%, trong khi da 10% san liyang 10a du cung cap liyang thtyc cho 60 trieu mai nam (Valent, 1990), a nhCrng vung nhiem neng, co the met hoar) toan nang suet (Cruz
-
Vazquez va cs., 2007). Doi vai h5u het cac vung trong lua a chau A, chau Phi va Nam M9, bien phap chinh hien nay de kiem soat benh deo on le sir dyng cac giOng 1Cia khang benh (Don va cs., 1999).Tuy nhien, viec sir dung giOng khang de quan 19 benh dao On ngay cang gip nhieu kho khan vi sty hinh thanh cac chUng gay benh mai (55 lam cho cac giong khang benh dao on chi co hieu luc trong yang 2-3 nam (Bonman va cs., 1992). Han nCra, doi vai nem deo On trong dieu kien tty nhien, cac chi:mg khOng dOc (avirulence) co xu hu'ang chuyen thanh cac chUng mang tinh dbc(virulence) (Lau va Ellingboe, 1993). Si' thay dOi dO da lam cho chien lu,ac‘ chon tao giong khang benh dao On g5p rat nhieu kho khan. De hieu diyac ca the khang cOa giong khang benh dao on, cac. nghien cCru ye mac 130 da dang di truyen cCia quan the nam P. grisea trong mot vung sinh thai cu the la rat quan trong (Levy va cs., 1993).
(5' Viet Nam, tinh da Ong di truyen cue quan the nem gay benh dao On a Bac bO va Nam IDO da duvc nhieu nha khoa hoc quan tam nghien cCru, tuy nhien a mien Trung cho den hien nay china CO bao cao chi bet nao ye phan loci cac chUng nem P. grisea, viec "Nghien cCru da Ong di truyen va xac dinh tinh dOc cua rn0t so chUng nam gay benh deo on a Nam Trung be" se cung cep thong tin ye dang dang nguOn gen va tinh dbc cua cac chung nem, phyc NA) cung tac tao giOng khang tai cac tinh Nam Trung bb.
2. VAT LIEU VA PHU'CYNG PHAP NGHIEN CCYU 2.1 Vat lieu nghien civu
Ching nam dao On: 120 mau benh deo On duvc thu th5p tir 3 tinh mien Nam Trung bb (Quang Nam, Quang Ngai, Binh Dinh) tren cac giOng khac nhau. Ket qua phan lap diyac 84 mau
Ket qua nghien ciru khoa hoc BVTV - S6 1/2016
benh, 18 mau phan lap khac nhau ye viing dia 19 va ten cac giong 10a khac nhau (bang 1) dixac chon de danh gia da dang di tuyen.
m6i
RAPD: 12 mai RAPD bieu hien da hinh(bang 2) duvc sir dung de danh gia kieu gen ciia cac chung vi khuan
Gi6ng 10a: tap doan 32 clang lua mang gen
khang benh ciao On khac nhau do vien lua IRRI
cung cep va giOng LTH (giOng chuAn nhiem) (bang 3) duvc sir dung a danh gia tinh dOc cua cac chung nem dao On.
- Dia diem: khoa N6ng hoc, tru,ong Dai hoc N6ng Lam Hue.
- Theri gian: tir thang 12/2014 den thang 8/2015.
Bang 1. Dia diem thu the)) cac cluing nem gay benh dao on
STT Ky hieu
K9 chi, Dia diem
1 QN1 BC15 Xa Tam Hiep,
huyen Niii Thanh, tinh Quang Nam 2 QN2 Xi23 Xa Tam
Thai,huyen Phil Ninh, tinh Quang Nam 3 QN3 0M4900 Xa Binh
An, huyenThang Binh, tinh Quang Nam 4 QN4 SV181 Xa Que
Phi), huyenQue San, tinh Quang Nam 5 QN5 KD18 X5 Binh Trung, huyen Thang Binh, tinh Quang Nam 6 QN6 BC15 xa Hwang An, huyen Que San, tinh Quang Nam 7 QNG1 Them 1 X5 Binh Chanh, huyen Binh San, tinh Quang Ngai 8 QNG2 KD18 xa Tinh Phong, huyen San Tinh, tinh Quang Ngai 9 QNG3 Q5 xa Binh Thanh, huyen Binh San, tinh Quang Ngai 10 QNG4 BC15 X5 Nghia Thirang, huyen Tu' Nghia, tinh Quang Ngai 11 QNG5 BC15 X5 arc Chanh, huyen MO Dirc, tinh Quang Ngai 12 QNG6 KD18 xa arc Hiep, huyen MO arc, tinh Quang Ngai 13 BD1 BC15 xa PhuVc An, huyen Tuy PhLrO,c, tinh Binh Dinh 14 BD2 KD28 X5 Cat Hanh, Huyen Phii Cat, tinh Binh Dinh 15 BD3 BC15 xa An Tu,o,ng Tay, huyen Hoai An, tinh Binh Dinh 16 B04 KD28 Xa Phu,bc LOc, huyen Tuy Phty&c, tinh Binh Dinh 17 BD5 Khang dan dOt bien X5 Nhan Tho, thi )(a An Nhan, tinh Binh Dinh 18 BD6 Q5 X5 Nhan Tan, thi xa An Nhan, tinh Binh Dinh
Bang 2. Ten cac mai RAPD sir dung de danh gia da dang di truy6n
STT Ten mai Trinh tu' mai
1 UBC#308 AGCGGCTAGG
2 UBC#310 GAGCCAGAAG
3 UBC#312 ACGGCGTCAC
4 UBC#317 CTAGGGGCTG
5 UBC#318 CGGAGAGCGA
6 UBC#338 CTGTGGCGGT
7 UBC#354 CTAGAGGCCG
8 UBC#364 GGCTCTCGCG
9 UBC#365 TAGACAGAGG
10 UBC#366 CCTGATTGCC
11 UBC#368 ACTTGTGCGG
12 UBC#383 GAGGCGCTGC
Bang 3. Danh sach cac d6ng Itia mang dun gien khang tham gia thi nghiem
STT GiOng Gen khang
Giong cho gen A
khang STT GiOng Gen
khang
GiOng cho gen khang 1 IRBLa-A Pia AICHI ASAHI 17 IRBLsh-B Pish BL 1
2 IRBLa-C Pia CO 39 18 IRBL1-CL P11 C101LAC
3 IRBLi-F5 Pii FUJISAKA 5 19 IRBL3-CP4 Pi3 C104PKT 4 IRBLks-F5 Pik-s FUJISAKA 5 20 IRBL5-M Pi5(t) RIL 249 (Moro.) 5 IRBLks-S Pik-s SHIN 2 21 IRBL7-M Pi7(t) RIL 29 (Moro.) 6 IRBLk-ka Pik KANTO 51 22 IRBL9-W Pi9 WHD-1S-75-1-127 7 IRBLkp-K60 Pik-p K 60 23 IRBL12-M Pi12(t) RIL 10
8 IRBLkh-K3 Pik-h K 3 24 IRBL19-A P119 AICHI ASAHI 9 IRBLz-Fu Piz FUKUNISHIKI 25 IRBLkm-Ts Pik-m TSUYUAKE 10 IRBLz5-CA Pi2(t) C101A51 26 IRBL20-1R24 P120 ARL 24 11 IRBLzt-T Piz-t TORIDE 1 27 IRBLta2-Pi Pita2 Pi No. 4 12 IRBLta-K1 Pi4(t) K1 28 IRBLta2-Re Pita2 REIHO 13 IRBLta-CT2 Pita C105TTP2L9 29 IRBLta-CP1 Pita C101PKT 14 IRBLb-B Pib BL 1 30 IRBL11-Zh Pi11(t) ZHAIYEQING
15 IRBLt-K59 Pit K 59 31 IRBLz5-CA Piz5 C101A51
16 IRBLsh-S Pish SHIN 2 32 Lijiangxintuanheigu (LTH) GiOng chan nhiem
2.2. Phu'o'ng phap nghien apu
• Phyang phap thu thap m3u benh, phan lap va nu6i cey nam dad On P. grisea:
Cac mau la va c6 bOng mang trieu chOng dien hinh cua benh dao On se duvc thu th5p tir cac ruOng 16a bi nhiem benh tai cac tinh Nam Trung BO (Quang Nam, Quang Ngai va Binh Dinh). Tien hanh phan 15p cac chim9 nem dao On tren moi tru'O'ng PDA va lam thuan bang phuvng phap thy clan bac tir (Burgess et al., 2008)
• Phvang. phap danh gia da clang di truyen cac cheing nem ciao On P. grisea:
Tach chiet ADN tong so cua nem theo phi.rong phap Kang va cs. (2003) CO cal tien. Sir dung 12 moi RAPD v61 chu trinh nhiet PCR:
biyac 1 gam 1 chu kji & 94°C trong 3 philt; buttc 2 96m 40 chu kjr 94°C/ 1phut, 37°C/1phOt, 72 C/ 2phOt; bu,O,c 3 Om 1 chu k9 6, 72°C/7phOt va cuOi cung u 6, 4°C. Dien di san ph5m RAPD - PCR tren agarose gel 1% va nhuOm b5ng SYBGR (10.000X).
Chi tieu theo dOi: SO moi bleu hien da hinh, tong sO bang duvc khuech dai cua m6i chi thi da hinh RAPD, tong so bang bieu hien da hinh cua moi chi thi da hinh RAPD, tong so bang bieu hien da hinh cua m6i chung.
Phyang phap xir ljt sO lieu: SO lieu RAPD duvc ghi nh5n dy'a vao thang Chun 100bp. Tieu chu5n h6a san ph5m RAPD theo quy Lift: "1"
xuet hien phan doan DNA, "0" khong xuet hien phan doan DNA. Cac sO lieu nay se duvc (Ara vao Chuang trinh NTSYS-pc de tinh ma tr5n twang dOng giCra cac doi
• Phuung phap lay nhiem nhan tao
- 18 chimg nem P. grisea thu th5p 6, 3 tinh Quang Nam, Quang Ngai va Binh Dinh nuoi thy tren mai tru,Ong Agar- bOt gao a nhiet dO 28°C/ 7 ngay de thu bao tir. Bao tir duvc thu bang nu,O,c cat vo trOng CO IDO sung them Gelatin (1%), de tang kha nang barn dinh cac bao tren la. Nang dO dung dich bao tir duvc sir dung de lay nhiem 16 105 bac)
- BO tri thi nghiem:
+ 32 d6ng lua don gien duvc trong trong cac Chau nhcra vai kith thLrac 20 x 20cm va bO tri theo khOi hoan toan ngeu nhien (RCBD) vad 3 Ihn nhac lai.
+ Lay nhiem b5ng phu,o,ng phap phun: Cay con a 4 tuen tu6i cua cac clang don gien duvc trong trong cac Chau nhy,a. SO, dung binh phun CO ap Ityc khoang 3atm/cm2 de phun cho lua. Dat cac chau nhy,a trong nha phun giCY em thirong xuyen trong khoang 16-24h, va nhiet dO khoang 24-26°C/6 ngay. Cay lua se dugs kiem tra sau 7 ngay lay nhiem, di,ra tren thang diem danh gia cua Kato (1993):
+ CAp 0: Khong CO vet benh, khang cao (HR) + CAp 1: Vet benh 16 mOt chArn nho b5ng deu kim, khang (R)
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+ Cep 2: Vet benh to han mau nau nhat den nau t6i,,khang (R)
+ Cap 3: Vet benh to han va CO mau xam giCra, nhiem (S)
+ Cap 4: Vet benh dac trumg hinh thoi, nhiem Ong (HS)
3. KET QUA VA THAO LUAN
3.1 Danh gia da dang di truy4n cua cac chung nam dao on P. grisea
TOng 12 moi RAPD bieu hien da hinh diyac sir dung de danh gia kieu gen cua 18 chUng nam dao on. SO doan DNA duvc khuech dai dao Ong tir 30 67 doan, tong so DNA khuech dai la 681 doan (bang 4).
Bang 4 cho they trong s6 12 moi RAPD, s6 doan ADN khuech dai cua 18 chUng nam dao On vai moi UBC#354 la nhieu nhet (67 doan) va moi UBC#318 la it nhet (30 doan). Trong 18 chi:mg nam thi chung thu thap & Thang Binh, Quang Nam (QN3) co nhieu doan ADN khuech dai nhet (49 doan) va chUng QNG3 (thu tai Binh San, Quang Ngai) co s6 doan khuech dai it nhet (23 doan). Tinh da hinh the hien su xu'at hien hay khOng xu6t hien cua cac phan doan DNA khi so sank giCra cac chUng nam vai nhau trong cling 1 mai. O' day, cac chung nam thu thap tai cac tinh Nam Trung BO cho they su' da hinh hoan toan giCra cac chiing (ti' le
cac doan DNA khuech dai dat 100%).
Quan he di truyen giCra cac gi6ng 10a nghien ci:ru duvc phan tich bang phan mem NTSYS 2.1, , tb, do xac Binh he so twang d6ng di truyen va sa do hinh cay ye moi quan he di truyen giCra cac chung nem (hinh 2).
Ket qua thu duvc duo tren sa do cay phan nhom cho they neu xet mirc d6 twang d6ng di truyen cua 18 chUng nam dao On & 0,53 thi se du'ac chia thanh 4 nh6m chinh va cac nhOm phu nhu' sau:
Whom A: Om 4 chung thu thap tO,
cac vung trong Ilia a QuOng Ngai (QNG1, QNG3, QNG4, QNG5) CO mire dO tuvng d6ng nam trong khoang 0,58 — 0,77. Trong nhOm A cac chUng lei duvc chia thanh 2 nhOm phu vai khoang cach
ditruyen gen han nhu, sau:
- Nhorn Al: g6m 2 chUng QNG1 va QNG3 co he s6 krang d6ng cao nhat la 0,77. Hai chi:mg nay thu duvc tren 2 gi6ng Ilia khac nhau (Thum- 05) nhumg deu tir mot vung sinh thai (Binh San, Quang Ngai) nen quan he di truyen cua chung kha gen gui;
- Nhorn A2: Nam trong khoang tu'ang d6ng tir 0,71-0,72 bao Om 2 chUng QNG4 va QNG5 dust thu thap hir 2 huyen khac nhau cua tinh Quang Ng5i la MO arc va Tu' Nghia, tuy nhien tren cling mot doi twang ky chii la , giOng 10a BC15 nen van g 'an nhau ye mat di truyen.
Bang 4. Tong
so
doan ADN duvc khu6ch dai cua 18 cluingnam
ciao on khi phan tich vai 12 mai RAPDMO QNG1 QNG2 QNG3 QNG4 QNG5 QNG6 QN1 QN2 QN3 QN4 QN5 QN6 B01 B02 BD3 804 B05 8D6
Tbng so doan AND
khuyech Qat
UBC#308 3 6 1 3 2 3 3 6 3 4 3 1 5 3 6 5 4 2 63
UBC#310 2 5 0 1 3 6 2 3 2 2 1 2 2 2 2 3 5 3 46
UBC#312 4 3 3 2 3 2 4 5 4 4 2 4 2 4 3 4 4 60
UBC#317 3 2 3 2 2 3 2 3 4 4 4 2 4 2 4 3 3 3 53
UBC#318 1 1 0 2 2 4 1 0 2 3 1 2 1 3 3 2 1 1 30
UBC#338 1 4 3 4 4 3 3 2 6 6 4 6 4 6 6 0 1 3 66
UBC#354 3 4 1 4 6 3 3 3 4 6 2 6 5 6 4 3 4 0 67
UBC#364 4 4 5 4 4 3 3 4 3 1 4 3 3 5 3 4 4 2 63
UBC#365 3 2 4 3 1 5 4 1 3 3 2 3 0 3 3 2 3 2 47
UBC#366 0 3 0 4 6 4 4 3 5 4 2 4 3 2 2 3 5 5 59
UBC#368 0 3 3 3 4 4 4 1 6 4 4 5 3 7 5 0 2 3 61
UBC8383 1 3 0 5 6 4 4 4 6 5 2 4 3 2 2 4 6 5 66
Tong 25 40 23 37 43 45 35 34 49 46 33 40 37 43 44 32 42 33 681
NhOm B: gam 4 chung CO mirc tkrang dOng tir 0,58 - 0,76, duvc chia thanh 2 nhOm phki:
- NhOm B1: co 3 chOng QNG2, QNG6, QN2, tren nhOm nay till, chiing QN2 (Quang Nam) nam rieng mot nhanh, mk".rc do twang dOng vai 2 chUng kia (Quang Ngai) la kha xa, va ghi nhan he so ttrang quan di truyen la 0,66 — 0,76;
- NhOm B2: chi g6m mat chUng QN2 vai mirc do twang dOng la 0,59
*NhOm C: g6m 8 chOng, co mirc tuang dOng tir 0,63 - 0,74 va duvc chia thanh 3 nhOm phu vai khoang cach di truyen gan han nhu' sau:
- NI-16m C1: CO mirc d6 twang dOng di truyen nam trong khoang 0,69 — 0,70 Om cac chung QN3 va QN5, clay la 2 chking thu thap duvc tren 2 giOng lua khac nhau nhu'ng cOng mat vOng sinh thai (Thang Binh, Quang Nam) nen chung co quan he di truyen kha gan nhau;
- NhOm C2: g6m cac chung QN4 (Que San, Quang Nam), BD1 (Tuy Phkr&c, Binh Dinh) va BDD4 (Tuy Phuttc, Binh Dinh), trong nhOm nay QN4 nam rieng mot nhanh, co moi quan he di truyen vai hai chCing kia kha xa nhau (0,67). Hai chOng BD1 va BD4 deu duvc thu thap cOng mot vOng sinh trial nen co he s6 twang dOng di truyen kha gan nhau 0,72 — 0,73:
- NhOm C3: gam 3 chiing QN6 (Que San, Quang Nam), BD2 (Phu Cat, Binh Dinh) va BD3 (Hoai An, Binh Dinh), he s6 twang dOng trong nhOm nay giao dOng 0,67 — 0,72. Trong do, chung nam QN6 nam hang mat nhanh vai sky tkrang dOng vai 2 chUng con lai kha thap (0,67), 2 chking BD2 va BD3 &roc thu thap cOng mot vung sinh thai nen co mirc d6 twang dOng di truyen cao han (0,72).
*NilOm D: g6m 2 chiing BD5 va BD6 (An Nhan, Binh Dinh) vai d6 tu'ang dOng la 0,74. 2 chOng nay thu thPp Van 2 giOng 10a khac nhau nhu'ng tir mat vOng sinh thai nen quan he di truyen ckia chung kha gan gal.
Qua cac ket qua nghien ciru, chung toi nhan thy cac chung nam P. grisea dtrqc phan lap tren cac giOng lua khac nhau tai cac tinh Nam Trung b0 CO scr khac biet kha Ian ye mat di truyen, dieu nay gap phan lam ca sa cho chien Vac nghien ciru lai tao giOng khang benh dao on tai cac tinh Nam Trung b6.
1 1 4 6 6 7 II I 10 11 12 1) 14 15 54 17 II
Hinh 1. Ket qua PCR 18 chung nem dad on vai UBC#338
(M: 100bp DNA ladder; 1-QNG1, 2-QNG2, 3-QNG3, 4- QNG4, 5-QNG5, 6-QNG6, 7-QN1, 8-QN2, 9-QN3, 10- QN4, 11-QN5, 12-QN6, 13-BD1, 14-BD2, 15-BD3, 16-
BD4, 17-B05, 18-BD6).
444
Hinh 2. Quan he ye di truyen giCra 18 chung nam dao on diya tren he so ttrang
dOng di truyen
3.2 Danh gia tinh (Ric cua cac chUng nam dao On
De phyc vu cho cOng tac chon tao giOng chung benh dao On thi trutec het can phai xac dinh duac tinh dOc cua cac chOng nam, CO the cac chking nam deu duac phan lap tir mau cay benh, CO sai nam va bac tir giong nhau nhu'ng tinh d6c khac nhau nen kha nang gay benh cOng se khac nhau. Dina vao cay di truyen (hinh 2) chung tei orlon 9 chung nam (isolate) thu0c cac nhom di truyen khac nhau
a
tien hanh lay nhie'rn nhan tao nham muc dich danh gia tinh d6c cua cac chOng nam nay. Ket qua lay nhiem va danh gia &Tic trinh bay a bang 5.Ket qua nghien ct:ru khoa hoc BVTV - S6 1/2016 Bang 5. Tinh dOc cua cac cluing nam gay benh dao on
TT Giong
Gen khang
ChUng nam dao on
QN2 QN4 QN6 QNG2 QNG3 QNG5 BD1 BD2 BD6
1 IRBLa-A Pia S S S S R S S R S
2 IRBLa-C Pia R S S S S S S HS S
3 IRBLi-F5 Pii R R R S S R R S R
4 IRBLks-F5 Pik-s R S S R S R S S R
5 IRBLks-S Pik-s R S S S R S S S S
6 IRBLk-ka Pik R R R R R S R R R
7 IRBLkp-K60 Pik-p R S S R R R R R R
8 IRBLkh-K3 Pik-h R R S R R R R R R
9 IRBLz-Fu Piz R R R S R S R S S
10 IRBLz5-CA Piz5 R S HR R R R R HR R
11 IRBLzt-T Piz-t S S S R R R R R R
12 IRBLta-K1 Pita HS S S S S S S R S
13 IRBLta-CT2 Pita R S R HR R R R R S
14 IRBLb-B Pib S S R R HR HR R R S
15 IRBLt-K59 Pit HR R HR R R S S R R
16 IRBLsh-S Pish R R R R R R R R S
17 IRBLsh-B Pish R R R S R R S R R
18 IRBL1-CL Pil S S S R R R R R R
19 IRBL3-CP4 Pi3 S S S S S R R R S
20 IRBL5-M Pi5(t) S S S R R R R HR R
21 IRBL7-M Pi7(t) S R R S R R R HR R
22 IRBL9-W Pi9 R HR R S R R S R S
23 IRBL12-M Pi12(t) S HS S R R R R R R
24 IRBL19-A P119 S S S S R R R R S
25 IRBLkm-Ts Pik-m R R R S S R R S S
26 IRBL20-1R24 Pi20 HS S S S S S S S S
27 IRBLta2-Pi Pita2 R R R R S R R HR R ,
28 IRBLta2-Re Pita2 S S R S R HR R R R
29 IRBLta-CP1 Pita R HR S S R S R R HR
30 IRBL11-Zh Pill(t) R R S S S R S R R
31 IRBLz5-CA Piz5 R R R S S S S S R
T9 le R/S 19/11 14/17 15/16 14/17 21/10 21/10 20/11 23/8 18/13
32 LTH HS HS HS HS HS HS HS HS HS
Ket qua er bang 5 cho thay chting nem co mirc d6 nhiern tren cac dong 10a mang gen khang rat khac nhau phu thu'c vao xuat X l.1
cua
vung sinh thai cung nhu' giOng Ida (cac ky chi)) khac nhau.Trong do, cac chin-1g nam duvc phan lap tai Quang Nam CO dOc tinh cao nhat v6i t9 le khang/nhiem that) giao dOng tir 14R/17S den 19R/11S, tiep den là cac chUng nem duvc phan lap tai Quang Ngai, cac chUng nam phan lap tt:r Binh Dinh CO d0c tinh thap nhat t9 le khang/nhiem cao (18R/13S — 23R/8S). Trong so 9 chUng nem sir dung trong thi nghiem lay nhiern, chUng CO d6c tinh manh nhat la QN4(Que San, Quang Nam) va QNG2 (San Tinh, Quang Ngai), chUng CO doc tinh that) nhat 16 BD2 (Phi, Cat, Binh Dinh).
Qua so sanh sty phan nhom ye kieu gen ve dOc tinh cua cac chCing nam nghien cUu, to thay cac chung co sty sai khac ye kieu gen thi dOc tinh cua chi -1g cOng.khOng giOng nhau (bang 5, hinh 2). Ket qua nay cho thay CO sty lien ket giCra kieu gen
va
kieu hinh lien quan den tinh da dang di truyen cua cac chi -1g nem dao On phan lap a Nam Trung B6.4. KET LUAN
- Sir dung 12 mOi RAPD .da hinh de nghien
ciru da clang di truyen ciia 18 chiing nem P.
grisea da cho they cac chiing nem cO sty da clang di truyen cao the hien qua so bang ADN trung binh/mOi le 56,75.
- 18 chung nem nghien CCI'U da ducec phan nhorn the hien b cay phan loai dkra tren he sO tvang clang. 18 chung nem dao On duvc chia thanh 4 nhOm chinh va nhieu nh6m phy, CO min dO tuang dOng dao dOng tit. 0,53 — 0,77. Trong do, cac nh6m A2, C2 va D CO he sO twang dOng kha cao.
- Bang phiyang phap lay nhiem nhan tao sir dung 9 chiing nem dao On cho they 2 chung nem QN4 (Que San, Quang Nam) va QNG2 (San Tjnh, Quang Ngai) CO dOc tinh cao nhat, chung co dOc tinh thep nhat la BD2 (Phii Cat, Binh Dinh).
TAI LIEU THAM KHAO
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Phan bran: TS. Ngo Vinh
Vien
PHAT HIEN, TUYEN CHON VA DINH DANH LOAI NAM
Trichoderma asperellum DOI KHANG vol VI KHUAN GAY BENH HEO XANH VI KHUAN Raltsonia solanacearum HAI 67 VUNG THU'A THIEN HUE
Isolation, Selection and Identification of Antagonistic Trichoderma asperellum to Bacterial Wilt Disease Raltsonia solanacearum on
Chilli Pepper in Thua Thien Hue Region
Trin Thi Thu Hal, VO Thi Mai Huvng2 va Huang Thi H6ng Qu61
Ngay nhOn bai: 10.11.2015 Ngay ch6p nhOn: 26.11.2015
Abstract
Three strains of bacterial wilt (R1, R2 and R3) caused by R. solanacearum were isolated from typical symptom disease of chilli pepper plants and tested pathogenicity of these three strains by artificial inoculation.
The selected strain R. solanacearum R1 with the highest pathogenicity was tested antagonistic ability to the isolated strains of Trichoderma. One hundred ,twelve strains of Trichoderma were isolated from 16 soil 1. Truteng Dai Hoc N6ng Lam, Dai Flpc Hue samples from Thua Thien Hue, Quang Nam and Binh 2. Trtr6ng DO Hoc Khoa Hoc, Dai HQC Hue Dinh province. Of which, number of strain with high
