• Nghien cu-u - Ky thuat
Danh gia tac dung chong s6c phan ve va chong ngira cua cao dac EZ tren thirc nghiem
Ngnyin Manh Ibyln'", Phiing Hoa Binh' Pham Thi Van Anh', Nguyin Phirolig Thanh'
'Tnrang Bai hpc Duo'c Hd Noi,•Tnrcmg Bpi hpc YHa Npi 'E-mail: nmanhluyem Summary
. n . ^ ! , " ' " ' ' ^ / ' * ^ i ' ^ T "^ ^ '"^' """"^ " ^ ^"^'^ '°r sntumrgk aaMms by the models of f ^ f ! ^ ^ •w^"^f^**^'^°"'^"™'''"*J^'"'^=**=»''*''"»fcO'e»ctsonanapMax/s
lemedym the regulaton of immediate-type allergic reaction. oiiraus iiom c zKeywortfe: EZ remedy, viscous extract, anH-alleigic, anaphylaxis, pniritus.
Cao dac duvc bio chi tir dich chiet nuoc bai thuoc EZ (goi tat li cao EZ-Hfi): Can rl6ng tOng dppc lieu da dupc xp ly dat tieu chuan theo khoi Ippng tppng ling vfti 5 thang (300 g dppc liSu). Chiit xuat bing nppc thee phppng phap sac, dun sil 3 l i n , m i l lan 1 gift, Ippng npftc g i p 6 lan dppc 116u Lpc ncng qua vai gac, ly tam loai tap chit Ip liing va gpp dich chiet. Co d j c thu dppc 73.8 g cao dac, hay 1 g cao dac tppng ling vfti 4,1 g dup-c Il6u.
Hoa chit thuoc thu: Tic nhan gSy di ling Compound 48/80. Sigma Aldnch, Singapore;
Methylprednlsolon (Medrpl®), vlSn nSn 4 mg' Pfizer, My: Ketotifen (Ketosan®), vlen nSn 1 mgi Hasan-Demiaphami.
Dong vat nghiSn cuu
ChuOt^nhit trang chiing Swiss, thuan chiing, ca hai giong, nang 25 ± 2 gam do VIpn V§ sinh Dich te Tmng ppng cung c i p . Dpng vpt dppc nuoi trong dieu kipn phong thi nghi6m cua B(i mon Dppc ly, Trppng Dai hpc 'y Ha N i l tii 5- 7 ngay trpftc khi nghiSn ciiu va trong suit thfti gian nghiSn cpu.
PhiKwig phap nghien ciiu
Danh gia tic dung ching shock phan vf t^
60 chupt nhit trang, chia ngau nhi6n lam 6 16 m i l 16 10 con.
L6 1 (Md hlnh): cho uing nuftc cit, ti6m mang bgnp dung dich compound 48/80 pha trong npftc muoi sinh ly (gpi tat la dd compound 48/80) Iliu 8 mg/kg can nang chu6t (/kg).
L6 2 (Chiing duvng): cho uing methylprednlsolon lieu 12 mg/kg, tl6m mang bgng dd compound 48/80 lieu 8 mg/kg.
Dat van de
Kim ngan dang, nuc nao, hoe hoa, hoang ba... la nhiing dppc lieu dppc biit d i n nhp nhu'ng thao dppc c6 hoat tinh ching viem, ching oxy hda «. Bai thuic EZ dppc xSy dpng dpa trSn CP sft ly luan y hpc cp Injyen kit hpp vfti cac kit qua nghiSn ciiu thpc nghISm vfti thanh phin chu dupc la cac v| thuic kim ngan ding, nuc nac, hoe hoa nham myc dich diiu tri cac trieu chiing bpnh vi6m da cp dia. Cac nghl6n ciiu da cdng b i cho thiy: cao chiet ethanol vS cao chiit npftc EZ co tac dung Wiang vl6m c i p va mpn tinh " « . Nghien oiu nay Hep tgc nham mgc tieu danh gia tac dgng ching sic phan vp va ching ngiia do tac nhan gSy di ling ggy ra tren thpc nghISm cOa cao dac EZ.
Ooi tippng va phirong phap nghiSn cihi Nguyen ll^u nghien ciiu
Bil thuic EZgim: Kim ngSn dang (Cap/is cum fblbm Lonlcerae). nuc nac (Cortex Omxyli), hoS hoa (Fhs Styphnolobii Japonkii imaturi), thppng nhi tft (Fruclus Xanthii stnimarii), hoang ba (Cortex Pheikidendn) dat tl6u chuan DDVN IV.
Cao dfc duvc bao chi tir dich chiit ethanol bil thuic EZ (ggl tat li cao EZ-EtOH): Can rieng tftng dppc Ilpu da dppc xp IJ dpt ti6u chuin thep khii Ippng tirang Png vfti 5 thang (300 g dupc lipu). Chiit hpl Ipu ri6ng tung vi bing ethanol 70%
3 lan, m i l lin 2 gift vfti Ippng ethanpl g i p 5 lan dppc Il6u. Phii hpp dich chiit, c i t thu h i l dung m6i, ly tam l i y d|ch. C6 dSc tren each thiiy thu dppc 40gcaodac,hay1gcaodactppngdppnq 7,5 g dupc liSu.
T * P CHi Dt;<;(C HQC - 9/2016 ( S 6 485 NAM 56)
• Nghien ciru - Ky thuat
L6 3 (Cao EZ-Hp Iliu thip): cho uong cao EZ-H^O lieu 3,5 g/kg (tuwng u n g 14,4 g d u g c lieu (DLykg), tiem mang bung dd compound 48/80 Ii4u 8mgfl(g.
L6 4 (Cao EZ-Hp liiu cao): cho uong cao EZ- Hp lieu 10,5 g/kg (hrcmg ling 43,2 g DUkg), M m m^ng bung dd compound 48/80 l i l u 8 mg/kg.
L6 5 (Cao EZ-EtOH Iliu thip): cho uong cao EZ-EtOH l i l u 1.92 g/kg (tuwng iJng 14,4 g DUkg, ti§m m^ng bung dd compound 48/80 lieu 8 mg/kg.
L6 6 (Cao EZ-EtOH lieu cao): cho uong cao E2-EtOH l i l u 5,76 gflcg (tmyng ijng 43.2 g DL/kg).
tiem mang bung dd compound 48/80 l i l u 8 mg/kg.
Dinh gii: T^ le p h l n tram chuOt chet 1 giiD- sau khi tiem Compound 48/80.
Banh gia tac dyng ching ngipa '^
80 chupt nhat t r l n g du-o-c chia n g l u nhien ISm 8 16, moi 16 10 con, chu6t du-gc cho dDng cac m l u thuoc thi> trong 4 ngay lien tiep, den ngay thLF 5 sau khi cho dung t h u l c t h i i 1 gib-, chuot du-gc tiem du-o-i da c6 {b cCing mot vj tri cho mSi ChuOt) tac nhSn gSy ngi>a dd compound 48/80 pha trong n u ^ c muoi sinh ly l i l u 4 mg/kg ngo?i tn> 16 cht>ng sinh hpc du'O'c tiem nu-o-c m u l i sinh ly dong the tfch.
L6 1 (Chung sinh hgc): c^o u6ng nu-ac cat 0,2 mL/10g, tiem du-^i da co nu-ac m u l i sinh ly.
Ld 2 (M6 hlnh): cho uong nu^i-c c i t 0,2 mL/lOg chuOt, ti6m du-o-i da c6 dd compound 48/80.
Ld 3 (Chung duxmg 1): cho u i n g methylprednlsolon 6 mg/kg, tiem dm^i da co dd compound 48/80.
L& 4 (L6 Chung duong 2): cho uong ketotifen 1 mg/kg, tiem dunS-i da co dd compound 48/80.
L6 5 (Cao EZ-Hp liiu thip): cho u i n g cao EZ- K O lieu 3,5 g/kg, tiSm d u a l da co dd compound
Ld6 (Cao EZ-Hp liiu cao): cho uong cao EZ- 4 8 ^ 0 •'°'S S'^9- «em 6ir6\ da c6 dd compound LdJ (Cao EZ-EtOH liiu thip): cho uing cao EZ-EtOH lieu 1,92 gfltg, tiem difai da c6 dd compound 48/80.
c J - u . ^ / ^ f ° ^Z-^fOH Iliu cao): cho u6ng cao EZ- S ^'^^' ^^'^ ^ " ^ ' ^^ ^ ^^ compound
Chi so nghidn CLFU: S I phan xa gai
toan bO CO- the chuQt d u ^ danh gia trong vong 20 phut, chia ra thanh cac giai dogn: 0-5 p h u t 5-10 phCrt; 10-15 va 15-20 phut, sau khi ti§m compound 48/80. S l l l n gai tnjng binh giQa cac 16 d cac giai doan va t i n g so l l n gai t u n g binh trong 20 phiit du-Q^ so sanh gi&a cac 16 de danh g i ^ tac dyng chong dj ij-ng.
Xu> ly s 6 li$u
Cac^so ligu thu th$p d u v c xu- ly bang phu-cng phap thong ke y sinh hpc theo T test - Student. K i t qua du-ffc trinh b i y d a n g : X ± SE. S^p khac bipt co y nghTa thong ke khi giS tn p < 0,05.
K i t qua nghien cuu
Tac d y n g c h o n g s o c phan v e cOa c a o E2 B a n g i : Tic dijng diong soc phan v$ cua cao EZ
tr§n thiK nghiim Lieu tfiif Lfl 1 (Ma hlnh)
Sdchugtchit _,,. ^ ., Igii^sautiem ^'^^"<''
compound ^ ' 48/BO •^•' L6 2 (Chi>ng dirong).
methyf-prednisoion L63((
thap)
EZ-HjOI(4u 3.5
1
L64 (cao EZ-HjO lieu 10,5 cao)
L6 5 (Cao EZ-EtOH lleuthgp) Lo 6 (Cao EZ-EtOH lieu C3Q)
1,92 gikg
Bang 2: Tic dpng chdng ngiia ciia cap dicEZ(n = 10) Ueuthir
0 16 mo hinh: Compound 48/80 l i i u 8 mg/kg tiem mang bung sau 1 gift gay tinh trang s i c phan v6 nang k h i i n 100% chupt c h i t .
L i chiing d p p n g s p dung methylprednlsolon ft Iliu 12 mg/kg, ty 1$ chupt c h i t 16 30%, t h i hi6n iS tac dpng c h i n g s i c phan vp. Cao EZ-H O 4 ca hai lieu thCr deu c6 kha nang lam giam ty 1^ c h u j t c h i t so vfti 16 mo hinh, t h i hl6n tac dpng c h i n g s i c phan y$. L i i u 3,5 g/kg lam giam ty 1$ c h i t nhieu hpn lieu 10,5 g/kg.
Cao EZ-EtOH ft ca hai I l i u t h i cung c6 kha nang lam giam ty 16 chu^t c h i t sp vfti 16 m i hinh, tuy nhl6n miJc d i giam it h p n sp vfti cao EZ-H O, t h i hien tac dung c h i n g s i c phan vp k6m h p n . '
Tac dyng c h i n g ngija cua cao EZ S6 /jn CO nhin xa ail trung hhh (X ± .tjF)
>5-10phffl >1Q-15phul >1&.20plia lo2(m6 iiinh: compoonri jasp)
Tina 9.111.5
T * P CHf DircJlC H p c - M 0 I 6 (S6 485 N A M 561
• Nghien CIJU - Ky thuat
l&3(chi)ng duong rnethytpresmsolon) Cmg/kg 3,1±0.7 L6 4 frilling duong ketot^n} 1mgfl(g 4,2±1,1 ID 51'cao EZ-H,0 Seu thap)
lAG{caoEZ-H,Oiieucao)
3,5 g/kg 3,2 ±0,8 iA7(caoEZ-BOHBeumap)
10.5 9/lg IfiB fcao EZ-EtOH Idu cao)
1,92 gftg 4.8 ±0,8 5,76 gftg 3.4 ±0,9 GW riiij; so wii A5 2 (K md hinh): '• p<0,(J5;"
& 15 chi>ng sinh hpc, khong SLP dung tac nhan gSy dj i>ng compound 48/80, kh6ng thay co chugt nao CO phin xa gai diln hinh trong 20 phut. 6 16 m6 hinh, ti^m du-oi da c6 dd compound 48/80, lilu 4 mg/kg co tac dicing gay di u-ng dien hinh th6ng qua chi s l phan xg gai trong ti>ng ttiai diem va ting s l sau 20 phdt.
Thulc chi>ng duo'ng methylprednlsolon lilu 6 mg/kg vS ketotifen 1 mg/kg co He dung giam s l phan xg gai ciJa chuot ro rpt trong 20 phiit theo doi sau khi ti6m compound 48/80 tao m6 hinh, qua do cho thay tac dyng chong dj u'ng (p < 0,05).
d 16 5: cho chupt uong cao EZ-H^O lieu 3,5 g/kg, s l lan gai trung binh trong ca 20 phOt giim ro ret c6 y nghTa thong ke so vo'i 16 m6 hinh (p < 0.01). TSc dgng c^ong ngiJa chu yeu tai 2 tho-i dilm 5-10 phiit (p < 0.05) va 15-20 phut (p < 0,01).
Cao EZ-Hp lilu 3.5 g/kg c6 tac dyng chlng ngiJa tirong dirang, th^m chf co xu hming tot hcyn v6i methylprednlsolon a lieu 6 mg/kg va ketotifen a lilu 1 mg/kg v^ t i t han & lilu 3,5 g/kg.
Cf 16 6: cho chupt uong cao E Z - H ^ lieu 10,5 g/kg, so lan gai trung binh trong ca 20 phiit giim ro r0t CO !? nghia thing kg so vai 16 m6 hinh (p < 0,01). Tic dyng chong ngi>a chii y l u tgi cic thd-i dilm 5-10 phiit (p < 0,01), 10-15 phiit (p <
0,05) v i 15-20 phiJt (p < 0.01). Cao EZ-Hp lilu 10.5 gfl<g c6 t i c dyng chlng ngu-a tu^ng du^cng, th§mchfc6xuhu^ngtlthffnv(yi methylprednlsolon a hlu 6 mg/kg v i ketotifen & lilu 1 mg/kg. Cao EZ- Hp lilu 10,5 g/kg co t i c t i c dyng tuwng 6uong v6i methylprednlsolon 6 mg/kg, ketotifen 1 mq/ka v i lieu 14,4 g/kg.
Q? ' ° ^" / ^ i ° ^^^^ "^"9 ^ ° ^ - E t O H lilu 1,92 g/kg, s6 lln g i i trung binh trong ca 20 phut giim ro r#t c6 y nghTa thing k6 so vol 16 m6 hinh {p^< 0,01). T i c dyng giam ngu-a chu yeu tai 2 thai diem 10-15 phiit (p < 0,05) v i 15-20 phtit (p < 0 01) Cao EZ-EtOH lilu 1,92 g/kg co t i c dyng twjng dirong vol methylprednlsolon 6 mg/kg v i ketotifen 1 mg/kg v i kh6ng thy^ sy khic bi^t so vai cao EZ- H p & ding lilu thCr (p > 0,05).
0 16 8: cho chupt uing cao EZ-EtOH lieu 5,76 g/kg, s6 l^n g i i trung binh trong ca 20 phiit giim ro r0t CO J? nghTa thing kg so voi 16 mo hinh (p < 0,01). T i c dyng giam ngi>a chu yeu tgi
T ^ CHi DUOC HOC - 9/20!6 (SO 485 NAM 56)
p<0.01: so vdi 16 4 (diung duong tetoftfe)."; p<0.05 cac thoi (Sem 5-10 philt (p < 0,05), 10-15 phiit {p < 0.05) va 15-20 phiit (p < 0,01). Cao EZ- EtOH 5,76 g/kg co t i c dyng tma-ng du^ng vol methi^prednisolon 6 mg/kg, ketotifen 1 mg/kg v i lilu 14.4 gflcg va tuong du-ang cao EZ-H,0 & cunq lieu thLF (p> 0,05).
Ban luan
T i c dung chong soc phan ve cQa cao EZ Nhilu nghien ci>u da cong b l c^o thay, compound 48/80 lilu 8 mg/kg tigm ming bung tr^ri c^u6t nhat trlng co the giy soc phan v# v i g i y chet chupt do lam tang hoat tfnh cac te bio mast a nhilu OT quan nhu- khi p h i quin, mach m i u d i n t6i giai phong q u i nhilu histamin i*^. Do d6, m6 hinh soc phan v? bang compound 48/80 trin chupt nhat tring thuwig duxj-c diing d l dinh gia t i c dyng chong dj iJng cap tinh cCia yiulc nghien ci>u i^.
K i t q u i nghien cu-u cho thiy, cao EZ-HO v i EZ-EtOH a ca 2 lieu thu- d i u co t i c dung chlng soc ro r^t Cao E Z - H p co xu hu-dng t i c dyng chong soc t i t han cao EZ-EtOH v i tu'ang du'ang vd'i methylprednlsolon, qua d6 t h l hi^n t i c dyng chong dj ting.
Tac dung chong ngu>a
^ Compound 48/80 thu'6'ng du-ac sii dyng v6i lieu thap 4 mg/kg, tiem du-di da co d l gay mo hinh ngCra tren chudt nhit trang. 6 lilu nay, iu^ng histarnin tilt ra khong du l i m chit chupt!
nhu-ng du de g i y ngu-a do chd y l u giai phong histamin & da '''•^. Trong nhOng phut dau lu'png histamin tang cao g i y ngu-a l i m chuOt gai liSn tyc, sau 15-20 phiit, histamin bj phin huy dan mat t i c dyng n^n phin xa ngt>a giam di. Vi ly do do. cac nghi6n CLFU n i y thu-frng dinh gia s l lan gai cua chupt trong tho-i gian 20 phiit sau khi ti6m cornpound 48/80. Cao E Z - H p v i EZ-EtOH & ca 2 lieu thu' vol lieu tuung i>ng vdi 14,4 v i 43.2 g DL/kg d i u c6 tac dyng chlng ngi>a do lam si'lan gai trung binh trong ca 20 phiit giim ro rpt c6 y nghia thong ke so vo'i 16 m6 hinh (p < 0,01), the hien t i c dyng chong dj i>ng. Tac dyng niy cua cao^dgc EZ tu-ang du'ang vd'i methylprednlsolon a lieu 6 mg/kg va ketotifen o- lieu 1 mg/kg.
Ket qua nghiSn CLFU nay phu hpp v6i cac cong b l ve t i e dyng chlng dj iJng ciia m0t s l vj thulc
y Nghien ciru - Ky thuat
chinh trong bai thulc. Djch chilt nu-ac cua kim n g i n d i n g co t i e dyng cai thien tinh trgng hen dj J i g do long trang tn>ng gay ra, i>c e h l hoat hoa t l bao mast do trypsin. Kim ngan d i n g thWng duwe dung trong chi>a tri cac bpnh man ngiJa.
may day, ban sai va eac benh di ling khic. Niic nac CO tac dyng chlng dj u-ng, lam t i n g su'c d l khang cua ca t h l . che pham tCF nuc nac du^c si>
dung de dilu tri bpnh vay n l n . m l day ra. Hoang ba thuwig du^e si> dung d l dieu trf c i c (^i>ng benh benh ngi>a, phii n l v i la m6t trong nam vi thulc thu-o-ng dygc SLF dyng dieu tri eczema o- Trung Quic f i .
Ket l u | n
Cao EZ-Hp v i EZ-EtOH a ca 2 lieu thi> (tuong duOTig v6i 14,4 g DL/kg va 43.2 g DL/kg) co t i c dyng chraig soc tren chupt nhit tring * mo hinh giy s i c bang compound 48/80.
Cao EZ-Hp v i EZ-EtOH a ca 2 lieu thij (tuwng du-ang v&i 14.4 g DUkg v i 43,2 g DLftg) co t i c dyng chong ngi>a trin chupt nhit fring a m6 hinh g i y ngiJa bing compound 48/80.
Tai lieu tham khao 1. Nguygn Mgnh Tuyen. PhCrng H6a Binh, Le Thi Minh v i OS. (2015). "Danh gia tac dyng chlng vi§m
thyc nghifm cOa cao d|c bao chi tiP djch chilt ethanol bai thuSc EZ". T?p chi Du%rc hpc, s6 7, 6-10.
2. Nguy^ Mgnh Tuyln. Phiing Hoa Binh, L§ Thj Minh v i CS. (2015), 'Oanh g i i t i c dyng chlng viem ttiyc nghi#m ciia cao d|c bio rfil tCr djch chilt nir&c bat thufic EZ", T^ip <^iDtfqc hoc. s l 10, 50-54.
3. Chandrashekhar V. M.. Halagali KS., Nidavani R.
B. (2011), "Anti-allergic activity of Gennan chamomile {Matricaria recutita L.) in mast cell mediated altefgy made]', J. Ethnophannacol. 137(1): 336-40.
4. H R Chitme. Mallikarjun Malipatil, V M Chandrashekhar and P M Prashant (2010). Antiallergic activity of Anstolot^i bracteolata Lank In animal model Indian Joumal of Experimental BMogy. Vol 48,46-52.
5. Jin-IWii Y. et al (2000). *lxeris dentata green sap inhibits both compound 48/80-induced anaphylaxis- like response and IgE-mediated anaphylactic response in munne model. Bioi Pharm. Bull, 25 (1). 5-9.
6. Wedemeyer J.. Tsai M., Glli S. J. (2000), "Roles of mast cells and basophils in innate and accquire immunity", Cun. Opin. Immunol., 12(6). 624-31
J- >^'an Y, F, Mao Q. Q.. |p s P, Lin Z. X.. Che C. T.
(2011), "Comparison on the anti-inflammatory effect of Cortex phellodendri Chlner^sis and Cortexpheliodendri Amurensis in 12-0-tetradecanoyl-phorbol-13-acetate- ' I " 3 7 ( 3 M I I 5 - 1 ™ ™ ' " " ' " • • •'• ^ " ' " ' " " " • ™ » » ' .
„ . . , ° ; , ° ' ^^''-''' f,"^^'' " ' " ' " 9 <^y Ihuic va vi lliuic Vi^t Nam, Nha xuat ban Y hpc. Hi Nil, 75-79 197- 199, 204-205, 298-300, 394-296 726-728 (Wgay nhan bii: 03/06/2016 - Ngiy duyit ding: 01/09/201S)
Nghien cmi xay dung tieu chuin chSt Iwcmg ciia du-o-c lieu tao meo (Fmctus Docyniae indicae)
Hoang Vi|t Dung'", Nguyin VSn B^ch', Pbam Birc Thjnh' Nguyin Hoing Hifp", Hoang Quynh Hoa'
'Hpc vipn Quany^TrudmgBpihpcBirpcHaNpi 'E-mail: vietdungk85<^yahoa.com
some qualifk^non criteria for S ' o S w ^ ^ c S T S ™ , ^ T * ^ f ^ ^"^ chamcterized by ashes, heavymetals, cnmMeJo Descnptxm. identifk:ation, tes on drying, quantHtatkm, total
Keywords: Docynia inOca, standaidizatkm.
^^^•T.. * ° f " " • ' " ^ ' ^ "^" ° " " i ^ " * " vClng rail phia _ u Viet Nam, pua cua hai Ici4l dy tio mto vi Bac thi ciy chua chat (Malus doumeri Bors A S L f f l ^ " ^ " ^ "^ * * " ' ISn trong 9pl ten Chev.) lai phan b6 kha han c h i . VI v j y trong thu^
a,ng nht, trong si dgng lam Ihu6c chua banh i « . t4, dugc l«u thutmg a , ^ nhan dan t a T » H ! ^ Trong kh, cay tao mec (Doc,.,a indk. pfl,all.) lam thu6c la qua L Ty tao m l ^uy h h ™
T * ' CHf D i r p c HQC - m 0 1 6 (SO 485 NAM 56?
