DINH DU'ONG VA T H U ^
Tuy nhien, cung giohg nhu bat cii sinh vat nao, nam ciing can cellulose, hemicellulo- se hoac cac nguon carbon khac cho su phat trien ciia chiing, san pham cuoi ciing la prote- in nain va CO,. Cac san pham cuoi ciing nay da Iam ma't mat VCK ciia rom so voi truoc khi ii na'm. Tuyen va ctv (2013) da bao cao ton that VCK dao dpng 4,5-26,2% khi ram duoc ii voi na'm Pleurotus eryngii. Do vay can co nhiing nghien ciiu sau rpng han ve hieu qua ciia viec sir dung nam trong che'bien thiic an giau chat xa noi chung, ba mia va loi ngo noi rieng truac khi ap dung vao san xua't chan nuoi. Ke't qua thi nghiem cho tha'y nong do na'm sii' dung de il ba mia va loi ngo kho thich hgp la 3%, thoi gian ii thich hgp la 3 tuan.
4. KET LUAN
Ba mia va loi ngo KH vai 4% hay 3% ure ke't hpp 2% voi deu cho san pham KH co gia tri pH dat tren 9, dam miii amoniac, co the bao quan duoc trong thoi gian dai toi 60 ngay
Nong dp na'm sir dung de ii ba mia va loi ngo kho thich hgp la 3%, thai gian u thich hgp la 3 tuan. Ba mia va loi ngo sau khi li voi chiing nam Pleurotus eryngii 3 tuan, ham lupng CP da tang tuong ung 102 va 112% so voi ban dau, ham luong lignin giam tuong iing 22,8 va 29,8%
Can tieh hanh thi nghiem nuoi duong cac khau phan co sii' dung ba mia va loi ngo dugc xii' ly bang KH 4% hay 3% ure ke't hop 2% voi va il na'm de danh gia nang sua't va hieu qua chan nuoi.
TAI LIEU THAIVl KHAO ^ ^^^^ous . H a r l e y R.D. a . d Jories E. ^ ^ ^ l ^ ' X . s . t , m "^
a m m o n i a a n d other alkalis on "^^ ' ,_<|g barlev Straw. J. Sci. Food & Agr., 29W ' ^ ^ ^ B.Q.
2 H u y e r , N . T , T u y e t Le ^ - J » " ^ « , „ « - / " s (2019a), F e r m e n t i n g n e e straw with the n^^6^^^ ^ ^ the erynpi mcreased the content °^ f'fij^.^s, for R^ral digestibilitj' of the straw. '-''•'^'""^?,^//w^vwlrrd-org/
Development 31, Article 125 t^^V' lrrd31/2/nfhuv31025 html j ^ „ X u a n . . N g u y e n TW H u y e n , B " * Q ' " ' ; § N ^ y ; „ Thi T u y e t N g h i e n , N g u y e n T h i Bieh T h u y ^^ ' » / ^ ^ ^ ^^^^y, Le (2019b). Effect of Using F " " ? ^ ' . , ' ^^d Microbial m S h e e p Diet on Nutrients Digestiom j ^ ^ ^ ^^ ^ ^ ^ 3 / Protein SjTithesis Asian J. Anim-
^'^^•^"'^ . ^ K* R;iaK T I.P., H e n d r i k s 4 . K u i i k S . J . A . V . , S o n n e n b e r g A S M . , « a J ^ ^ ^ ^ ^ ^ ^^
W.H. a n d C o n e J.W. (2015) ^ " y ^ ^ i ^ p e a e s , biomass Importance ot rung _ f _^
hgnocellulosic colonizahon and time
degradability. Anim FeedSa
x ly kiem a ty le b e u hoa imposition a n d in vitro I Tech., 209 40-50 ACM B a a r s l . I - P . , H e n d r i k s 5. Kuijk S.J.A.V., S o n n e n b e r g A.S M., Baars j . j ,
^ , r - J r l - d t - . i r ? h o " T e s r ^ ^ ^ ^ Umversity, T h e Netherlands, ISBN 978-94-6257-654-4:
133^9-
6. N g u y e n Xuan Tr?ch (2004) Anh h u o n g c hoa bang v o i / h o a c u r e d e h luong an vac "
r o m . T a p chf C h a n n u o i , 11(69) 16-18
7. Tuyen V.D., P h u o n g H.N., Cone J W . , B a a r s J.J.P., S o n n e n b e r g A . S . M . a n d H e n d r i k s W . H . (2012), Interaction b e t w e e n fungus a n d fibrous a g n c u l t u r a l b y - p r o d u c t on chemical composition and »i i'ltro r u m e n f e r m e n t a h o n a n d m e t h a n e p r o d u c h o n Bioresource Technology, D O P 10.1016/| biortech 2012 02.001.
o. T u y e n D.V., P h u o n g H N, C o n e J.W., B a a r s J.JP., S o n n e n b e r g A . S . M . a n d H e n d r i k s W.H. (2013). Effect of fungal t r e a t m e n t s of fibrous a g n c u j h i r a l b y - p r o d u c t s on chemical c o m p o s i t i o n a n d in v i t r o r u m e n fermentation and m e t h a n e p r o d u c t i o n Bioresource Tech., 129; 256-63 9. Van S o e s t P.J., R o b e r t s o n J.B. a n d L e w i s B.A. (1991).
M e t h o d s for d i e t a r y hber, n e u t r a l d e t e r g e n t fiber, a n d n o n starch p o l y s a c c h a r i d e s in relation lo aninnal nutrition. J.
Dair>' Sci, 74 3583-97
B O S U N G CHE P H A M ^ '
I H o c v i e n c a o h o c T r u o n g i ) ! ' .,
• T n r o n g D^i hoc N o n g L a m B.ii: C • n ' H Q C vien N o n g n g h i e p Vici N a m ' T a c gia lien he PCS TS. D a n g Thuy \ i n N a m , Trau Quy, Gia Lam. Ha Noi Dien ti
T C H I E T U A T .^.~i L O N C O ^ . iin riiir. Ooan Phuwig Thuy^ vd Dn";,' , / mung^*
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UlNH DUONG VA THlTC AN CHAN NUOI
T O M T A T
Nghien ciHi d u a c tieh h a n h nh5m d a n h gia hieu qua ciia viec bo sung probiotic duoi dang che'pham Actisaf va chat chiet xuat thao d u a c duoi d a n g che'pham Herbiotic vao khau p h a n thii'c an lon con sau cai siia 2 giai doan 3-6 va 6-10 tuan tuoi. Do'i vcd timg giai doan, chon 210 lon con la con lai F|(DxLY) khoe manh, d o n g deu, p h a n n g i u nhien vao 716:16 DC, TNI, TN2, TN3, TN4.
TN5 va TN6, moi 16 30 con. Cac thi nghiem deu du'oc lap lai 3 lan. Lon con 16 DC an khau p h a n ca so (KPCS), T N I ' KPCS+0,025% Herbiotic, TN2: KPCS+0,05'% Herbiotic, TN3: KPCS+0,05% Actisaf, TN4: KPCS+0,1% Actisaf, TN5: KPCS+0,025% Herbiotic va 0,05% Actisaf, TN6: KPCS+0,05% Her- biotic va 0,1% Actisaf. Ke't qua cho tha'y: Bo sung Actisaf, Herbiotic hoac ke't h o p ca 2 loai nay vao khau phan lgn con trong 2 giai d o a n 3-6 t u a n tuoi va 6-10 tuan tuoi deu co tac d u n g tang kha n a n g thu n h a n thiic an, cai thien toe dp sinh truong, giam tieu ton thiic an, giam ty le tieu chay, tie't kiem duoc phi phi thirc an va thuoc thu y. Bo sung Actisaf to ra co hi^u qua h o n so voi bo sung Herbiotic.
Bo sung ke't h a p 0,025% Herbiotic vai 0,05% Actisaf ciing co tac d u n g t u a n g t u n h u b o sung Actisaf voi lieu 0,1%. Bo sung Herbiotic va Actisaf vao khau p h a n lon con giai doan 3-6 tuan tuoi mang lai hieu qua cao b a n so voi giai d o a n 6-10 tuan tuoi.
Tir khoa: Actisaf, Herbiotic, khdu phan dn, sinh trudng lon con, tieu chay lan con.
ABSTRACT
A d d i t i o n a l probiotics a n d H e r b preparation into the diet of piglets T!ie study was conducted to evaluate the effectiveness of the addition of probiotics in the form of Achsaf preparation a n d herbal extracts in the form of Herbiotic preparation to the diets of piglets after weaning in 2 stages' 3 to 6 weeks of age and 6 to 10 weeks of age. For each stage, choosing healthy and uniform 210 piglets of Fl(DxLY) crossbred, randomly assigned to 7 lots: DC, TNI, TN2, TN3, TN4. TN5 and TN6, 30 piglets per each. The expenments were repeated 3 times.
The piglets were fed the basic diet (BD), T N I : BD+0,025% Herbiotic, TN2: BD+0.05% Herbiotic, TN3: BD+0.05% Actisaf, TN4: BD+0,1% Achsaf, TN5. BD+0.025% Herbiotic and 0.05% preparations, TN6: BD+0.05% Herbiotic and 0.1% Actisaf. The results showed that supplementing with Actisaf, Herbiotic or combining these 2 preparations into piglet diet in the period of 3-6 weeks of age as well as 6-10 weeks of age increased the ability of feed receive, improved growth rate, reduced FCR, reduced diarrhea rate, saved on feed a n d veterinary drug costs. Actisaf supplements proved to be more effective than Herbiotic supplements. Supplementmg a combination of 0,025% Herbiotic with 0.05% Actisaf had the same effect as a d d i n g Actisaf at a dose of 0.1%. A d d i n g Herbiotic and Actisaf to piglet diets from 3 to 6 weeks of age was more effective than 6 to 10 weeks of age.
Keywords: Actisaf, Herbiotic, diet, piglet growth, piglet diarrhea.
I.DATVANDE d i i n g . T h a y t h e ' k h a n g s i n h b a n g cac cha't c6 , . , , . . , , , , , .., tac d u n e cai t h i e n n a n g sua't v a siic k h o e v a t Lcm con v o i h e t i e u h o a c h u a p h a t t n e n - . ' , , .* . . . . .^ , -
;, ,„ „ „• , s J , , „ , , , 3 , n u o i n h u : p r o b i o t i c s , p r e b i o t i c s , axit h u u c o h o a n t h i e n n e n r a t d e m a c cac b e n h v e d u o n g
tieu h o a , n g o a i r a k h a n a n g d i e u h o a t h a n v a c h a t chie't xua't t h a o d u g c , . . . d e b o s u n g v a o , . . . , • . *- , I , . - 1.1.-1, t h u c a n c h a n n u o i la cac giai p h a p h u u h i e u nhiet k e m , s u e d e k h a n g c u a co t h e c h u a c a o , .-, n e n d e m a c b e n h . M o t t r o n g n h i j n g b i e n p h a p ( T u n g v a P e t t i g r e w , 2006).
p h o b i e h d u o c sir d u n g t r o n g n h i e u n a m q u a N h i e u n g h i e n ciiu d a c h i m g m i n h tac la b o s u n g k h a n g s i n h v a o k h a u p h a n a n l o n d u n g co lgi ciia p r o b i o t i c v e siic k h o e va n a n g con n h a m n g a n n g i r a cac b e n h d u o n g t i e u s u a t s i n h t r u a n g ciia v a t n u o i { P a p a t s i r o s v a h o a , kich t h i c h t a n g t r u o n g , n a n g c a o h i e u ctv, 2009). C a c c h e p h a m chiet x u a t tir t h u c q u a sir d u n g t h i i c a n ciia lon c o n Tuy n h i e n , v a t co tac d u n g t a n g c u o n g ha'p t h u c h a t d i n h viec sir d u n g k h a n g s i n h t r o n g t h u c a n g a y r a d u o n g , cai t h i e n k h a n a n g t i e u h o a , kich t h i c h h i e n t u g n g vi k h u a n k h a n g k h a n g s i n h , m a t s u p h a t t r i e n c u a cac vi k h u a n co loi t r o n g k h a c t o n d u k h a n g s i n h l a m a n h h u d n g de'n d u o n g r u o t n h o d o h a n che' t i e u c h a y 6 v a t a n t o a n t h u c p h a m v a s u e k h o e n g u o i t i e u n u o i . S e y y e d n e j a d va M o t a m e d i (2010) d a
KHKT Chdn nuoi so 256 - thdng 4 ndm 2020
[ DU'ONG VA THUC A N U I A N W ^ ^
chung minh thao dugc co tinh an toan sinh hpc, khong co tac dung phu va chua tim tha'y vi khuan khang thuo'c.
Trong nhiing nam gan day, nhieu che' pham probiotic va chat chie't xua't thao dugc da CO mat tren thi truang thirc an chan nuoi ciia nuoc ta. Nhieu nghien ciiu nham danh gia hieu qua cua viec bo sung cac che pham nay vao khau phan thiic an lgn con da dugc thuc hien. Biii Van Dinh va Dang Thiiy Nhung (2015), Le Van An va ctv (2017) da nghien ciiu bo sung cac che'pham probiotic vao khau phan an lgn sau cai siia va Ign thit. La Van Kinh va ctv (2012) bo sung cac che'pham cha't chiet xua't thao dugc vao khau phan an ciia lon con.
Nghien ciiu nay dugc thuc hien nham danh gia hieu qua ciia viec bo sung probiotic duoi dang che'pham Actisaf STD va chat chiet xua't thao dugc duoi dang che'pham Herbiotic vao khau phan thiic an lon con sau cai siia gom 2 giai doan: 3 de'n 6 tuan tuoi va 6 deh 10 tuan tuoi.
2 . VAT LI|U VA PHUONG PHAP NGHIEN CUTU 2.1. V | t lieu
Vat heu nghien ciiu gom: che pham Herbiotic la san pham chiet xuat thao dugc ciia cong ty Indian Herbs Saharanpur (An Do), che'pham Actisaf STD la san pham pro- biotic ciia cong ty Phileo Animal CARE Lesaf- fre (CH Phap). Thi nghiem (TN) nuoi duong tie'n hanh doi voi Ign con Iai Du x (LY) tir 3 de'n 6 tuan tuoi va tir 6 deh 10 tuan tuoi t^i Trai Chan nuoi Truong Dai hgc Nong Lam BSc Giang, til thang 6/2019 den thang 10/2019.
2.2. Phuong phap
Doi vdi ton con 3-6 tuan tuoi: Lua chgn 210 lgn con sau cai siia luc 21 ngay tuoi, la con lai Du X (LY), khoe manh, dong deu, phan ngau nhien vao 7 16: 15 DC, TNI, TN2, TN3, TN4.
TN5 va TN6, m5i 16 30 con. Thi nghiem dugc lap lai 3 lan, tong so lgn thi nghiem la 630 con.
Doi vdi lan con tu 7 deh 10 tuan tuo}: Lua chgn 210 lon con luc 42 ngay hioi, la con lai Du X (LY), khoe manh, dong deu, phan ngau
nhien vao 7 16: 16 DC, TNI, TN2, TN3, ^ 4 . TN5 va TN6, m6i 16 30 con. Thi ngh'^m dugc lap lai 3 Ian, tong so Ign thi nghie nia 630 con.
Khau phan"sir dung cho 16 DC va cac 16 TN ciia lgn con a ca 2 giai doan nhu- sau.
DC: khau phan ca sa (KPCS), TW;
KPCS+0,025% Herbiotic, TN2: KPCS+0^5/o Herbiotic, TN3: KPCS+t),05% Achsaf, TNJ:
KPCS+0,1% Actisaf, TN5- KPCS+0,025X>
Herbiotic va 0,05% Actisaf, TN6: KPCS+0,05 /o Herbiotic va 0,1% Actisaf.
Nguyen lieu thiic an (TA) dugc lay mau theo tieu chuah Viet Nam (TCVN4_325-2007), phan tich thanh phan hoa hoc cacmau nguyen lieu TA tai Phong Phan tich TA ciia Cong ty Co phan Dinh duong Hai Thinh theo cac phuong phap ciia TCVN
Tren co so ket qua phan tich thanh phan hoa hgc cac nguyen lieu TA va Tieu chuan an ciia lgn con theo NRC (2012), xay dung cac cong thuc TA cho lon TN bang phan mem BriU.
Lgn con dugc nuoi trong 6 chuong san be t6ng, cac chuong co num uong tu' dong va mang an thu cong. Trong chuong co he th6'ng den suoi, he thong lam mat tu dgng. Lgn con dugc tiem vaccine day dii theo quy trinh a i a c6ng ty.
Cdc chi tieu theo doi
Khoi lugng (KL) lon con 6 3, 6 va 10 tuan tuoi; TA thu nhan (TATN), tieu ton TA (TTTA) va chi phi TA a cac giai doan 3-6 va 6-10 tuan tijoi; ti le tieu chay a cac giai doan tren.
Xir ly solieu
Cac so lieu dugc xii ly thong ke bSng phan mem Exel 2010, tinh cac tham so thong ke- gia tri trung binh (Mean), sai so tieu chuan (SE) Sir dung Minitab 16 de kiem dinh gia thiet thong ke bang phan tich phuong sai 1 yeu to 3.KETQUiiVATHA0LUAN
3.1. Anh huong cua viec b6 sung che pham vao khau phan ciia Ign con tii 3 den 6 tuln tuoi
3.1.1. Khoi luang ca the ciia lcm con jwi doan til 3 den 6 turn tuoi
42
KHKTChan nuoi su 256 - thang 4 nam 2020UiiNM U U O N G VA T H U ' C A N C H A N N U O I
Tai thoi diem bit dau TN, KL lgn 6 cac 16 khong sai khac co y nghia thong ke (P>0,05) (Bang 1). Ket thuc TN, KL lon Iiic 6 tiaan tuoi 6 16 TN4, TN5 va TN6 dat cao nhat, hrong img la 15,98; 15,96 va 15,98 kg/con; tiep theo la 16 TN3: dat 15,35 kg/con; cac 16 TNI va TN2
tuong ling la 15,12 va 15,35 kg/con va thap nha't la 16 doi chiing, dat 14,79 kg/con.
Khac biet giii'a cac 16 TN4, TN5 va TN6 la kh6ng CO y nghia thong ke (P>0,05). Khac biet giiia 16 TNI va TN2 cung khong co y nghia thong ke (P>0,05).
Bang 1. Khoi lugng ca the lgn con tir 3 den 6 tuan tuoi (MeanlSE, n=90/16, kg/con) Tuan tuoi DC TNI TN2 TN3 TN4 TN.^i Tl
6,64±0,19 14,79^±0,08
6,58±0,13 15,12^:0,09
6,62±0,22 15,35^+0,08
6,60±0,16 15,82^+0,06
6,61±0,31 15,98"±0,07
6,62±0,26 15,96UO,04
6,62±0,26 15,98^0,04
Ghi chii: Cdc gid tri trung binh tren cdng hang co cdc chir a, b, c, d khde nhau Id sai khde co y nghia thong ke (P<0,05)
Nhu vay, viec bo sung Herbiotic voi lieu Iugng 0,025 va 0,05% da giup Ion con dat KL cao hon so voi khong bo sung 0,33-0,56 kg/con hoac 2,23-3,79% (P<0,05). Bo sung Actisaf voi lieu 0,05% da giiip Ign con dat kh6i Iugng cao hon so voi kh6ng bo sung 1,03 kg/con hoac 6,96% {P<0,05). Bo sung Actisaf voi lieu 0,1%
ciing nhu bo sung ke't hgp Actisaf, Herbiotic voi cac lieu tuong iing la 0,05; 0,25 va 0,1;
0,05% da giiip lgn con dat kh6'i Iugng cao hon so voi kh6ng bo sung 1,17-1,19 kg/con hoac 7,91-8,05% (P<0,05).
Cac ke't qua neu tren phii hgp voi mgt so' nghien ciiu ve viec bo sung cac che' pham probiotic 6 nuoc ta trong thoi gian gan day:
Le Van An va ctv (2017), thii' nghiem bo sung probiotic voi miic SxlO"* CFU/g vao khau phan an cua Ign con giai doan sau cai sira da lam cho toe do sinh truong cao gap 1,23 lan so voi do'i chung kh6ng bo sung. Bo sung probiotic duoi dang che' pham Fubon vo'i lieu 0,05%
vao khau phan an da lam cho lgn con sau cai sira CO to'c do sinh truong cao hon 13% so vol kh6ng bo sung (Dang Thiiy Nhung va Doan Van Soan, 2017).
Bang 2. Lugng thiic an thu
3.1.2. Thu'c dn thu nhdn, tieu to'n vd chi phi thuc dn
Da CO nhiing su khac biet nha't dinh ve lugng TATN trung binh hang ngay giiia 16 DC va cac 16 TN (Bang 2). Cac che'pham probiotic va thao dugc da co tac dung tang cuong hoat dgng tieu hoa cua lgn con. Nguyen nhan la do tac dung co lgi ciia probiotic ve sue khoe va nang sua't sinh truong ciia vat nuoi (Papatsiros va ctv, 2009). Roselli va ctv (2005) da chiing minh rang probiotic co tac dung tich cue d6'i voi qua trinh tieu hoa bang each tang hoat dgng ciia cac enzyme vi sinh vat trong probiotic; tang kha nang mien dich bang each kich thich he th6'ng mien dich va tai sinh ciia niem mac ruot. Cos va ctv (2006) cho rang cac che' pham chie't xua't tir thuc vat co tac dung tang cuong hap thu cha't dinh duong, cai thien kha nang tieu hoa, lam tang tinh them an ciia vat nu6i. Tang kha nang heu hoa, tang tinh them an chinh la nguyen nhan Iam tang kha nang thu nhan thuc an cua Ign con khi duoc bo sung probiotic hoac chat chie't xua't thuc vat. Chinh vi vay, thu nhan thuc an ciia lgn con trong cac 16 thi nghiem cao hon ro ret so vol 16 d6i chiing.
nhan va tieu ton thiic an
Chi fieu DC TNI TN2 TN3 TN4 TN5 TN6
TATN, g/con/ngay 519,764:5,67 525,38''±5,63 528,56^''±5,42 532,36=±5.38 533,12''+5,28 533,09-^±5,32 534,09^5,33 TKL, g/con/ngay 370,45''±0,15 388,18'±0,13 396,82^:0,12 4I9,09''±0,13 425,9]4i),14 424,55>+0,I4 425,45^^,13 TTTA, kg TA/kg TKL 1,40-^,03 1,35''±0,02 1,33^±0,02 1,27^±0,02 1,25'±D,01 1,26'=+0,01 1,26'^±0,02 Gia thanh TA, d/kg 14 583 14.658 14.733 14 683 14.783 14.758 14.833 Chi phi TA, d/kgTKL 20 460 19 839 19.624 18.651 18 504 18.531 18.620
KHKT Chdn nuoi so 256 - thdng 4 ndm 2020
D I N H D U O N G VA I H U L AIN LTT
CKANNyel
Mac dii Iugng TATT hang ngay oia Ign con trong cac 16 TN deu cao hon DC, nhung do miic TKL cua lgn con trong cac 16 nay khac biet nhieu so voi DC nen TTTA giira cac 16 TN va DC la khac biet ro reL Bo sung Herbiotic voi lieu Iugng 0,025 va 0,5% vao khau phan an Ign con giai doan 3 de'n 6 tuan tuoi da giup lgn con co miic tieu ton thiic an thap hon so voi kh6ng bo sung 0,05kg TA/kg TKL, tuong duong voi giam dugc 3,53-5,06% TTTA (P<0,05). Bo sung Herbiotic voi lieu luong 0,05% cung nhu Actisaf heu 0,05 hoac 0,1%, hoac ket hop Actisaf, Herbiotic vdi cac lieu tiiong ling la 0,05; 0,25 va 0,1; 0,05% deu giiip Ign con co miic TTTA tha'p hon so voi kh6ng bo sung 0,13-0,15kg TA/kg TKL, tuong duong voi giam duoc 9,46-10,78% TTTA (P<0,05).
Khong CO su chenh lech ve miic TTTA giira cac each bo sung khac nhau nay (P>0,05).
Yuan va ctv (2001) da chi ra rang bo sung 0,5"/^i thao dugc gom vo cam, quyt, bot hat qua th6ng co the cai thien dugc 4,5% hieu qua chuyen hoa thiic an. Mgt vai nghien cuu a nuoc ta dang xac nhan bo sung cac che' pham probiotic vao khau phan an ciia lon con se giam dugc TTTA: Dang Minh Phuoc (2010) khi sir dung probiotic bo sung vao khau phan an ciia Ion con giai doan tir 28-56 ngay tuoi da cho bie't TTTA la 1,50 trong khi do doi chimg khong bo sung la 1,55kg TA/kg TKL.
Mac du gia thanh ctia TA khi bo sung cac che' pham Herbiotic hoac Actisaf cao hon so voi TA khong bo sung, nhung do mite TTTA/
kg TKL thap hon nen chi phi TA/kg TKL ciia cac 16 thi nghiem deu thap hon so voi DC.
Chenh lech ve chi phi TA/kg TKL cua lgn con Bang 3. Hieu qua kinh te ve chi
trong cac 16 TNI, TN2, TN3, TN4. TN5 va TN6 deu thap hon so voi 16 DC lan laat la J, * ' 10, 9 va 9%.
3.1.3. Tinh hinh mdc tieu chitj cim Itm " « Cac so lieu theo doi tinh hinh mac tieu chay cho thay: Bo sung Herbiotic vm lieu luong 0,025 va 0,5% da giup Im con co ty le mSc Heu chay giim tha'p hon so vol khotig bo sung hi 5,6 den 9,0%, Bo sung Achsaf heu 0,05 hoac 0,1% da giiJp lon con CO ty le mac heu chay giam thap hon so voi khong bo sung 13%. B6 sung ke't hop Actisaf, HerbioHc vm cac lieu hiong iing la 0,05; 0,25 va 0,1; 0,05%
deu giiip lon con co ty le mk tieu chay giam tha'p hon so vm khong bo sung 13%. Dieu nay dugc ly giai boi co che tac dpng ciia probiotic va chat chief xuat thao duoc khi vao trong duong ruot da lie che vi sinh vat co hai, phat trien vi sinh vat CO loi o duong ruot nen han che' duoc hoi chiing tieu chay o lon. Hojberg va ctv (2001) da chiing minh r^ng bo sung che'pham thao duoc Ecodiar (tinh dau fu cay Oregano) giam duoc 20% fy le Heu chay so voi khong bo sung. Dang Thiiy Nhung va Doan Van Soan (2017) da fhii nghiem bo sung 0,03 va 0,05% Fubon vao thiic an cho lon con giai doan sau cai sira nhan tha'y h le tieu chay da giam 17% so voi 16 khong bo sung probiotic.
3.1.4. Sa bo uoc tinh hieu qud kinh techi phi thiic dn vd thuoc dieu tri
Cac so'lieu hnh toan (Bang 3) cho tha'y: So voi 16 DC, cac 15 TNI, TN2, TN3, TN4, TN5 va TN6 CO KL hung binli lang cao hon, TTTA va chi phi TA/kg TKL, chi phi fhu6c dieu hi hoi chiing tieu chay tha'p hon. Do do, tong dii phi TA va thuoc dieu hi 6 cac 16 TN deu tha'p hon 16 DC.
phi thiic an va thuoc dieu tri Chi lieu Iheo doi
Khoi luong bat dau (kg/con) Khoi luong kei thiic (kg/con) Khoi lu-gng tang (kg/con) Chi phi thiic an (d/kg TKL) Chi phi thiic an (d/con) Chi phi thuoc dieu tn (d/con) Tong chi (d/con) Tong chi (d/kg TKL) Tie't kiem so vm DC (d/kg TKL)
6,64 14,/9 8,15 20 460 166.749 26.000 213.209 26.161
6,58 15,12 8,54 19 839 169.425 20.000 209.264 24 504 1.657
6,62 15.35 8,73 19.624 171.318 17.500 208.442 23.877 2.284
6.6 15,82
9,22 18 651 171.962 12 000 202 613 21.975 4.185
6,61 15,98 9.37 18,504 173 382 12 000 203 886 21.75')
4.41)1 6,62 15,96 9,34 173osn
12 000 203.611 21.800 4.361
is 620
44
KHKT Chdn nuoi \6 256- thdng 4 nam 2020
UINH DUONG VA THtTC AN CHAN NUOI
3.2. Anh hu6ng cua viec bo sung che pham vao khau phan ciia lgn con giai doan tir 6 den 10 tuan tuoi
3.2.1. Kho'i luang ca the cua lan con
Tai tho'i diem bat dau thi nghiem, KL lgn a cac 16 kh6ng co su sai khac co y nghia thong ke (P>0,05) (Bang 4). Ket thuc thi nghiem, KL lgn luc 10 tuan tuoi 6 16 TN4 va TN6 dat cao nhat, tie'p theo la 16 TN3 va TN5, roi deh cac 16 TNI va TN2 va thap nhat la 16 DC, Khac biet giii'a cac 16 TN4 va TN6 la khong c6 y nghia th6hg ke (P>0,05). Khac biet giua 16 TN3 va TN5 ciing nhu TNI va TN2 deu khong co y nghia th6'ng ke (P>0,05). Khac biet giua cac 16 Bang 4. Khoi lugng cff
TNI, TN2, TN3, TN4, TN5 va TN6 so vai 16 DC la CO y nghJa thong ke (P<0,05).
Nhu vay, bo sung Herbiotic voi lieu lugng 0,025 va 0,05% da giup lpn con dat KL cao hon so voi khong bo sung 0,46-0,75 kg/con hoac 1,52-2,48% (P<0,05). Bo sung Actisaf voi lieu 0,05% ciing nhu bo sung dong thoi Actisaf voi lieu 0,05% va Herbiotics vo'i lieu 0,025%
da giup Ion con dat khoi lupng cao hon so voi kh6ng bo sung 1,26-1,34 kg/con hoac 4,17- 4,43% (P<0,05). Bo sung AcHsaf voi lieu 0,1%
ciing nhu bo sung ke't hgp Actisaf, Herbiotic vol cac lieu tuong iing la 0,1; 0,05% da giiip lpn con dat KL cao hon so vol khong bo sung 1,46-1,50 kg/con hoac 4,83-4,96% (P<0,05).
the lgn con (kg/con)
Tuan luoi6 10
DC 15,72±0,05 30,22'lD,07
TNI 15,75+0,04 30,68'±0,06
TN2 15,67l«,01 30,97'±D,09
TN3 15,69±0,03 31,56''±0,05
TN4 15 60±0,07 31,68't0,08
TN5 15,06±0,05 31,48''±0,07
TN6 15.67±fl,06 31,72'±0,04
Dang Thiiy Nhung va ctv (2019) da bo
sung che pham Nor-Gutan (chie't xua't tir cam, chanh, nho va hat de) vod lieu 1 kg/1 tan thiic an va che' pham NSOAB9 (chie't xua't tii cam - chanh, re rau diep xoan, co ca-ri va cay Quillaja) voi lieu 1,2 kg/1 tan TA vao khau phan an ciia lpn thit tu 56 deh 76 ngay tuoi nang cao dupc KL so voi khong bo sung lan lugt la 1,28 va 1,05 kg/con; tuong iing voi 3,70 va 3,04%
3.2.2, Thu nhan vd tieu ton thiic an
Cac s6' lieu tren Bang 5 cho thay da co nhung su khac biet nha't dinh ve lugng TATN trung binh hang ngay giiia 16 DC va cac 16 TN.
Dieu nay chiing to cac che' pham probiotic va
thao dupc da co tac dung tang cuong hoat dgng tieu hoa ciia lpn con trong giai doan 6-10 tuan tuoi.
Theo Dang Thiiy Nhung va ctv (2019), cac 16 TNI (bo sung che'pham Nor-Gutan), TN2 (bo sung che' pham NSOAB9) co miic TNTA trung binh hang ngay lan lugt la 1,42 va 1,40 kg/con/ngay, trong khi do 16 DC khong bo sung CO miic thu nhan TA la 1,35 kg/con/ngay.
Mac dii lugng TATN hang ngay ciia lgn con trong cac 16 TN deu cao hon 16 DC, nhung do miic TKL trung binh hang ngay ciia lgn con trong cac 16 nay khac biet so voi do'i chiing nen TTTA giiia cac 16 TN va DC la khac biet.
Bang 5. Lugng thiic an thu nhan va tieu ton thiic an lgn con giai doan 6-10 tuan tuoi
Chi Heu DC T N 4 TN5 TN6
TATN, g/con/ngay 1,048,62^*0,52 1 066,74''±0,48 1,066,74^+0.46 1 096,51"±0,5] 1 097,64'+0,451,096,56''±0,5] 1.097,56-'±0,51 TKL g/con/ngay 537,04''±0,15 522,96'ifl,13 566,67<±0,12 587.78"±0.13 593,70'±0.14 588,15-^,14 594,44»±0,13 TTTA, kg TA/kg TKL :.95^±0,05 1,93''±0,04 1,91''±0.03 1.87=±0.02 1,85^+0,03 1,86'-±D,02 1.85'+0.03 Gia thanh TA, d/kg 7 000 7,075 7.150 7,100 7 200 7175 7.350 Chi phi TA, d/kg TKL 13.668 13 649 13 621 13 245 13.311 13.377 13 571
Bo sung Herbiotic voi lieu lupng 0,025%
da giiip lgn con co miic TTTA tha'p hon so voi khong bo sung 0,02kg TA/kg TKL, tuong duong voi giam duoc 1,2% TTTA, nhung sai khac la kh6ng co y nghia thong ke (P>0,05). Bo
sung Herbiotic voi lieu lugng 0,05% da giiip lpn con CO miic TTTA tha'p hon so voi kh6ng bo sung 0,05kg TA/kg TKL, tuong duong voi giam ducyc 2,44%i TTTA, sai khac la co y nghia thong ke (P<0,05). Bo sung Actisaf lieu 0,05
KHKT Chdn nuoi so 256 - thdng 4 ndm 2020 45
DINH DLfONG VA tHUCANCHANNU^l
hoac 0,1%, hoac ke't hgp Actisaf, Herbiotic voi cac heu tuong iing la 0,05; 0,25 va 0,1; 0,05%
deu giiip lgn con co miic TTTA tha'p hon so voi khong bo sung 0,09-0,llkg TA/kg TKL, tuong duong voi giam dugc 4,46-5,44% TTTA (F<0,05). Khong c6 su chenh lech ve miic TTTA giiia cac each bo sung khac nhau nay (P>0,05).
Theo Dang Thiiy Nhung va ctv (2019), cac 16 TNI (bo sung che'pham Nor-Gutan), TN2 (bo sung che' pham NS0AB9) co muc TTTA lan lugt la 2,01 va 1,93kg TA/kg TKL, trong khi do 16 DC kh6ng bo sung co miic TTTA la 1,94kg TA/kg TKL.
Mac dii gia thanh ciia thiic an khi bo sung cac che' pham Herbiotic hoac Actisaf cao hon so voi khong bo sung, nhung do miic TTTA/
kg TKL tha'p hon nen chi phi TA/kg TKL ciia cac 16 TN deu tha'p hon so vai 16 DC, Chenh lech ve chi phi TA/kg TKL ciia lgn con trong cac 16 TNI, TN2, TN3, TN4, TN5 va TN6 tha'p hon so voi 15 DC, lan lugt la 0,14; 0,34; 3,09;
2,61; 2,13 va 0,71%
3.2.3. Tinh hinh mac tieu chay
Cac so' lieu theo doi tinh hinh mac hgi chiing tieu chay ciia lgn con giai doan 6 de'n 10 tuan tuo'i cho tiia'y: Bo sung Herbiotic voi lieu Bang 6. Hi^u qua kinh te uoc tinh
lugng 0,025 va 0,5% da giup lgn con co ty^^
mic tieu chay giam thap hon so voi khong ^^
sung tir 2,22 deh 3,33%. Bo' sung Actisat neu 0,05 hoac 0,1% da giup Ipn con co ty le mac tieu chay giam tiiap hon so voi khong bo sung 3,33-4,44%. Bo sung ket hop Achsaf, Herbiotic voi cac lieu hiong ung la 0,05; 0,25 va 0,1;
0,05% deu giup lgn con co ty le mac heu diay giam tha'p hon so voi khong bo sung 5,56 /o.
Dieu nay dugc Iy giai boi co che tac dpng ciia Actisaf (Probiotic) va Herbiotic (chat diiet hi thuc vat) khi vao tiong duong mot da lie che'vi sinh vat co hai, phat tnen vi sinh vat co lgi a duong mgt nen han che duac hgi chung tieu chay o lgn.
3.2.4. Sa ho udc tinh hieu qud kinh techi phi thuc dn vd thuoc dieu tri
So lieu Bang 6 cho thay sau thoi gian TN, so voi 16 DC, cac 16 TN deu co KL trung binh tang cao hon, TTTA va chi phi TA/kg TKL thap hon va chi phi thuoc dieu hi hpi chiing tieu chay tha'p hon. To'ng cong ve cac chi phi TA va thuoc dieu tri, so voi 16 DC, cac 16 TNI, TN2, TN3, TN4 deu co chi phi thap hon, lan lupt la 250; 428; 827; 894; 960 va 758 dong/kg TKL.
tren chi phi thiic an va thuoc dieu tri Chi tieu theo doi
Khoi luong bat dau (kg/con) Kho'i luong ket thuc (kg/con) Khoi luong tang (kg/con) Chi phi thuc an (d/kg TKL) Chi phi thuc an (d/con) Chi phi thuoc dieu tri [d/lgn con) long chi (d/con) long chi (d/kg TKL) Tie't kiem so voi 16 DC (d/kg TKL)
DC 15,72 30,22 14.5 13 668 198.186 15 000 213 186
14.702 TNI 15,75 30,68 14,93 13 649 203 780 12 000 215.780 14 453
TN2 15.67 30.97 15,3 13 621 208 401 10.000 218 401 14.275
TN3 15,69 3L56 15,87 13 245 210.198 10 000 220 198 13,875 827
TN4 15,6 31.68 16,08 13 311 214.041 8.000 222 041 13.809
894 TN5 15,06 31,48 16,42 13 377 219 650 6 000 225 650 13.742
960 TN6 15,67 31.72 16,05 13 571 217.815 6.000 223.815
13 945 758 4. K£T LUAN
Bo sung probiotic, chat chie't xuat duoc thao hoac ke't hgp ca 2 loai nay vao khau phan lgn con trong 2 giai doan 3-6 va 6-10 tuan tuoi deu CO tac dung tang kha nang TNTA, cai thien toe d6 sinh tmong, giam TTTA, giam ty le tieu chay, tiet kiem dugc phi phi TA va thu6'c thii y.
Bo sung probiotic duoi dang che' pham
Achsaf to ra co hieu qua hon so voi bo sung chat chiet xua't dugc thao duoi dang che pha'm Herbiotic. Bo sung ket hgp probiotic vai chat chiet xuat thao moc cung co tac dung UuTug hr nhu bo sung AcHsaf voi lieu 0,1%. '
Bo sung probiotic va chat chiet xua't dupc thao vao khau phan lan con giai doan 3-6 tuan tuoi mang lai hieu qua cao hon so voi giai doan 6-10 tiiantiioi.
46 KHKT Chdn nuoi s<; 256 - thdng 4 nam 2020
D I N H D U O N G VA THU'C A N C H A N N U O I
TAI LIEU THAM KHAO probiotic dum dang che'pham Fubon vao khau phan . " „ . , , , ^ . . , cho lgn con theo me va sau cai sua Tap chi KHKT Chan 1 Le Van An, Nguyen Thi Loc, Nguyen Mmh Huong j . ^ ^ ; 225 40
vi Nguym Thi Thu Ttrng (2017) Ngh.ln a h . s« , ^^ .^^ Nhung, Ding Vi Ho, va Biu Vin Dinh dvng che ph.m prob.olK (Baallu, suUilis v . »rt.l,»a» p019). BS sung chfphSm Ihio du,c vio khfc phan an
«. ptaMra™) trong khau phan Ihuc an nuo, l>w giai ^ „ , ^ , „ , p o , , , ^^ ^,,j ^H^^^. ^.^J^ „„i,i^243(4) an sau rai sua va mini tViit Tan rhi K H r M WAno ^ ' 1- , v / doan sau cai sua va nuoi thit Tap chi KHCN Nong
nghiep, 1(2). 209-16.
Cos P., A.J. Vlietinck, D.V. Berghe and L. Maes (2006).
Anh-infective potential of natural products. How to develop a stronger in vitro 'proof of concept'. J
20-25
NRC (1998). Nutrients requirements of pigs. National Research Council, Academy Press Washington, DC, Papatsiros V.G., Tzika E.D., Papaioannou D.S., Alexopoulos C, Tassis P.D. and Kyriakis S.C. (2009) Ethnopharmacol, 106(3), 290-02. rtjcAupuuius v,., '•
'^ ' v/ Effect of origanum vulgans and allium sativum extracts 3 Bui Van Duih va Dang Thiiy Nhung (2015) Tac dong for the control of proliferative enteropathy in weaning
cua viec bo sung che pham Ien men lactic (Kulactic) vao pigs. Polish J. Vet. Sci,, 12(3). 407-14.
khau phan lon con tap an va cai sua, Tap chi KHCN jo. uj^g ^ i n h Phuoc (2010) Nghien ahi ung dyng mot Ctian nuoi, 2' 52-58. gg che'pham axit hiJu co, probiotic, thao duoc thay the 4 La Van Kinh (2012). Nghien cuu mot so che pham co khang sinh trong thuc an heo con cai sua. Luan an Tieh si nguon goc thao dupc trong chan nuoi lgn va gia cam Nong nghiep, Truong DH Nong Lam TR Ho Chi Minh Bao cao nghiem thu tai Vien Khoa hoc KI thuat Nong u . Tung CM. and Pettigrew J E. (2006) Cntical review nghiep mien Nam, 26/3/2012 of acidifiers. NaHonal Pork Board, Available at. http://
5 D^ng Thiiy Nhung va Do Thi Nga (2013) Bo sung www pork org/ Documents/PorkScience /ReviewOfA- che pham Bacillus enzyme (ProbioUc) cho lon con lai ddifiers.pdf. Accessed 10 April 2009 .
Landrace x Yorkshire tu tap an deh 56 ngay tuoi. Tap n . Seyyedneiad S.M. and Motamedi H. (2010). A review ehi KHCN Chan nuoi, 4 10-16 on Native medicmal Plant m Khuzestan, Iran with 6 Dang Thiiy Nhung va Doan Van So^n (2017) Bo sung Antibacterial properties Inf. J. Phar, 6, 551-60.
ANH H l / d N G CUA TY LE LYSINE TIEU HOA/ME DEN SINH TRLfdNG, DAY MCH LLfNG, TUOI THANH THUC SINH DUG VA HIEU QUA sif DUNG THLfC AN DOI v 6 l LON CAl HAU BI LVN
Trinh Hong San'*, Pham Duy Phdm'^, Nguyen Thi Huong^ vd Tran Thi Bich Nggc^
N g a y n h a n bai bao: 02/01/2020 - N g a y n h a n bai p h a n bien: 05/02/2020 N g a y bai b a o d u o c c h a p n h a n d a n g : 08/02/2020
T O M TAT
Nghien cmi a n h h u o n g ciia ti Ie Lysine tieu hoa/ME den kha n a n g sinh trirong, day m o lung, tuoi thanh thuc sinh due va hieu qua su d u n g thiic an doi voi lon cai hau bi LVN nuoi tai Tnong tam Nghien cuu lgn Thuy P h u o n g tu' thang 6 deh thang 12 nam 2019. Nghien cuu duoc tieh h a n h tren 90 lon cai h a u bi LVN, chia thanh 2 giai doan thi nghiem, giai doan t u 50 kg den 80 kg va giai doan tit 81 kg de'n khi phoi gio'ng lah dau, moi giai d o a n deu bo tri 3 m u c voi ti Ie Lysme heu hoa/
ME bang 95% NRC, 100% N R C va 105% NRC. M6i m u c Lysme bo tri 30 con/muc, chia thanh 3 Ian l | p lai, moi lan thi nghiem 10 con Ke't qua nghien ciiu cho tha'y, voi ty le Lysine m u c 95% NRC, 100% NRC va 105% NRC, tang khoi luong giai d o a n 50-80kg la 895,29; 877,38 va 879,88 g/ngay giai doan 81kg deh phoi gio'ng lan d a u la 557,06; 572,37 va 570,20 g/ngay Voi ty le Lysine tu'ong ung, day m o lung tai thoi diem 80kg la 11,40; 11,60; 11,70mm, tai thoi diem pho'i giong la 15,30; 15,20;
15,20mm. Tuoi dong due lan d a u t u o n g u n g voi cac m u c Lysme la 181,80,183,90 va 184,60 ngay. He so chuyen hoa thiic an vol miic Lysine t u o n g iing giai doan 50-80kg la 2,30; 2,31; 2,36kg va 81kg den phoi giong lan dau la 2,93; 2,91 va 2,91kg. N h u vay, ty le Lysine tieu hoa/ME trong khau phan an cua lgn cai h a u bi LVN da khong anh h u o n g de'n to'c do sinh truong, day m o l u n g va he so chuyeii hoa thiic an n h u n g anh h u o n g de'n tuoi d o n g due va phoi gio'ng lan dau.
Til' khoa' Ty le Lysine tieu hoa/ME, chuyen hod thuc dn, lan LVN.
' Tnmg tam Nghien cuu \<?n Thuy Phuong
^ Vien Chan nuoi
* Tac gia lien he: TS Trinh Hong Son, Trung tam nghien cuu lgn Thuy Phucmg, Vien Chan nuoi; DT' 0912792872, Email* [email protected]
