HOA HOC-GQN6 NGHE THtfC PHAM
NGHIEN c t f u QUA TRINH TRICH LY SAPONIN TRITERPENOID TONG Tl/
LA DINH LANG VOfi SlT H 6 TROf CUA KY THUAT SIEU AM
• HA TH! THANH NGA - TRl/dNG BAO NGHI - D 6 H 6 N G PHUONG THAO - TRAN CHI HAI
TOM TAT:
Trong nghi6n ciJu nay, chiing toi tien hanh Ichao sat anh hifdng cua ti le nguydn Heu: nifac, cong suSt sieu dm va thcfi gian sieu am tdi qua trinh trich ly saponin triterpenoid tong tLf la dinh lang Polysciasfruticosa (L) Harms. Ket qua cho thay ca 3 yeu to deu co tac dgng den ham lifdng saponin triterpenoid t6ng thu difdc theo quy luat h^m bac hai. Qu^ trinh toi Mu hoa bang phu'dng phap b l mat dap iJng da thu du'dc thfing so toi ifu nhu" thdi gian sieu am 20,75 phut, cong sud't sieu am 221,32 W/g, vdi ti 16 ni/dc: nguyen lieu la 29,50; luc nay ham lifdng saponin triterpenoid tong thu diTdc cao gl'p x^p xi 2,5 \in so vdi mSu khong difdc xu'ly sieu am.
Tif khoa: C6ng sua't sieu Sm, la dinh lang Polysciasfruticosa (L) Harms, thdi gian sieu am, ti le nguyen lieu: nifdc, saponin triterpenoid tong.
1. Gidi thi^u
Ngay nay, viec suf dung cac difdc pham co nguon go'c tif nhien dac biet la thifc vat quy de phong, chffa benh va boi bd cd the ngay cang difdc quan tam. M6t vai nghien cu'u cho tha'y cay dinh lang CO chu'a nhieu hdp cha't co hoat tinh sinh hoc gi6'ng nhan sam giiip phuc hoi su'c khoe. Dinh lang la x^ (Polyscias fruticosa (L) Harms, Araliaceae) CO nguon go'c d vilng dao Polyneise (Thai Binh Du'dng), phan bo' rai rac d vilng nhiet ddi va can nhiet ddi [1,2]. Dinh lang cd chiia cac alkaloid, glycoside, saponin cung cac acid amin tan trong nifdc nhrfBI, B2, B5, C, cac phytosterin. Ngoai ra, trong dinh lang con chu'a 20 acid amin vdi cac loai acid amin khong thay the du'dc nhif lysin, meihionin, tryptophan, cysterin [3]. Tat ca cac bo phan nhif re, than va la deu cd chu'a saponin nhifng
nhieu nhat la d vo rl va la [4]. Trong nhffng nam gan day, cac nha khoa hgc da tim tha'y tac dung sinh hoc cua dich chie't tff la dinh lang chu'a nhom saponin co phan genin, cau true tnterpen hay steroid 27 cacbon, cd tac dung tifeu ddm, tri ho, ldi tieu, ngffa cao huyet ap,.. [5].
Cd nhieu phu'dng phap ho trd cho qua trinh trich ly saponin trong nguyen lieu thifc vat nhff enzyme, vi sdng, COi sieu tdi han,... Tuy nhien, trong nghien effu nay, chung toi tie'n hanh danh gia anh hffdng cua mot so' thong so' (ti le nguyen lieu: nu'dc, cong suat sieu am, thdi gian siSu am) den ham lifdng saponin triterpenoid tong ttf la dinh lang trong qua trinh trich ly vdi sff hd trd cua .sdng sieu am. Day la khao sat tiin dl cho cac nghien effu sSu hdn ve vific khai thac va xac dinh hoat tinh ctia dich chie't tff la dinh lang.
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TAP CHi CONG THIfONG
2. Vat lieu va phuTdng phap nghi§n cihi 2.1. Vat lieu
Nguyen lieu la dinh lang la dai, rang cffa nho tai huyen Thanh Hda, tinh Long An. La dffdc thu hai, phdi khd tff nhi&n, xay, nghien va sang qua ray 0,3mm. Phan qua ray difdc bao quan trong cac tui zip va tranh anh sang trffc tie'p. Khi phan tich ban thanh ph^m nay, ke't qua thu dffdc do am xa'p xi 5%, tro tong xa'p xi 21 %, cacbohydrate xa'p xi 50%, lipid xa'p xi 8,5% va protein xa'p xi 18%.
Cac hda cha't dung trong nghien effu dat tieu chuan cbS't lu'dng cua hda chat phan tich phdng thi nghiSm.
2.2. PhiMigphdp nghien cdu
2.2.1. Trich ly saponin triterpenoid tdng vdi su ho tra cm song sieu dm
Nguyen lieu dffdc c&n chinh xac 2,000g phoi trpn vdi nffdc d cac ti le nguyen lieu: nffdc khao sat 1:10, 1:15, 1:20, 1 25, 1:30, 1:35, 1:40 (w/v) roi xff ly si6u Sm vdi cdng sud't co' dinh 225 W/g trong thdi gian 15 phiil. Ty le nguydn lieu: nffdc phu hdp du'dc chon de tien hanh nghien effu lan lifdt cac thdng s6' tiep theo nhff thdi gian sieu am (5, 10, 15, 20, 25 va 30 phut) va cong sua't sieu am (150, 187,5, 225, 265,5 va 300 W/g) de thu dffdc dich chifi't.
Ket thuc qua trinh trich ly, hdn hdp dffdc ly tam d nhiet do phdng vdi td'c do 5800 vdng/phut trong vdng 15 phut de thu lay phan dich trong va chuan hi cho qua trinh phan tich quang pho tren thie't bi Model photolab 6100 Vis cua WTW - Dffc.
2.2.2. Tdi uu hda qud trinh trich ly saponin triterpenoid tdng td Id dinh ldng
Qua trinh td'i ifu hda theo md hinh bl mat dap ffng dffdc sff dung de nghien effu qua trinh Erich ly saponin triterpenoid tdng so' tff la dinh lang vdi 3 yd'u to' la ti 16 nguyen Ii6u/nffde, cdng sua't sieu am va thdi gian sieu am cho ham muc tieu ham Iffdng saponin triterpenoid tdng so' vdi sff hd trd cua phan mem MODDE 5.0.
2.2.3. Xdc dinh hdm luang saponin triterpenoid tdng
Mau sau khi trich ly dffdc Ipc va pha loang 10 lan, hut 0,2 mL mau da pha loang vao d'ng nghiSm, them lan Iffdt la 0,2 mL vannihn - acetate (10%), 1,2 mL HCIO4, dun each thuy va u d 70''C trong 15 phiit. Sau 15 phut, cac d'ng nghiem dffdc lay ra lam mat trong 2 phut, ethyl acetate du'dc bd sung sao
cho tdng the tich la 5 mL. Tdng ham Iffdng saponin triterpenoid dUdc phan tich dffa tren phffdng phap quang phd so mau, do dp ha'p thu quang phd d bffdc sdng 548 nm vdi chat ehuan la Escin [6].
2.2.4. Phuang phdp xd ly so lieu
Trong nghien effu nay, mdi thi nghiem tie'n hanh lap lai 3 lan, ke't qua dffdc trinh bay dffdi dang gia tri trung binh ± do lech chuan. X\{ li sd lieu bang phan mem Microsoft Office Excel 2013, danh gia sff khac biet cd y nghia giffa cac nghiem thu'c dffdc thffc hJ6n bang phan mem Statgraphics Centurion 15.1 vdi dd tin cay 0,05 va ke't qua td'i ffu hda dffdc xff ly vdi phan mIm MODDE 5.0.
3. Ket qua va thao luan
3.1. Anh hudng ciia ti l^ nguyen lifu: niidc den hdm lU^ng saponin triterpenoid tdng tii Id dinh ldng
Bang 1. Anh hi/dng cua tl le nguyen lieu:
dung moi de'n hdm IUdng saponin triterpenoid tong TII0 nguyen ii$u:
nudc (w/v) 1 1 1 1 1 1 1 10 15 20 25 30 35 40
Ham liidng saponin Iriterpenold long (mg/g) Mail xi!lly sidu am
(0,42±0,01)' (0,68 ± 0,01)»
(0,92 + 0,02)=
(1,37 ±0,02)"
(1,56 ±0,02)' (1,54 ±0,02)' (1,51 ±0,03)'
Mau ifhong XLI ly sieu am (0,36 + 0,00)>
(0,52 ±0,01)' (0,56 ±0,02)=
(0,60 ±0,02)' (0,70 ±0,01 )•
(0,69 + 0,01 )•
(0,69±0,01)«
Trong cung mot col, cdc gid tri dUOc ddnh ddu bdi cdc chu cdi gidng nhau thi si( khde nhau khong cd j nghia ve mat thdng ki (a = 0,05).
Bang 1 cho thay khi tang dan tile nguydn lieu:
nffdc thi ham lu'dng saponin triiterpenoid tdng thu dffdc tang, tuy nhien den mdt gia tri ti 16 nao do thi viec gia tang lu'dng nffdc khdng lam thay ddi dang ke ham Iffdng chat chie't thu dffdc. Cu the, khi ti 16 nguyen lieu: nffdc tang tff 1:10 de'n 1:30 thi ham lu'dng saponin triterpenoid tang cao xa'p xi 3,7 lan; tuy nhien nd'u tie'p tuc tang len cac ti le 1:35 va 1:40, ham Iffdng thu saponin triterpenoid tdng giam nhe nhifng khdng dang ke. Ngoai ra, chiing tdi cung tie'n hanh thi nghiem vdi cac mlu khdng xff ly sieu am d cung dieu kien tffdng ffng.
Ke't qua cho thay, qua trinh trich ly vdi sff ho trd
HOA HOC-CONG NGHE THKC PHAM
cua sdng si6u am da mang lai nhffng hieu qua tich c\ic. Ham Iffdng saponin triterpenoid cua mlu dffdc xff ly sieu am cao hdn mlu khdng xff ly sieu am, dac biet tai tile nguyen lieu: nffdc la 1:30, gia tri nay dat xa'p xi 2,3 lan.
Theo Jian - Bmg va cgng sir, sff gia tang hieu sua't trich ly bang sdng si6u km la do hi6n tffdng sui hot tao nen life cat xen cao lam tang td'c do truyen khdi cua chat chie't. Ngoai ra, sif vd bpt cung tao nen sff khua'y trgn manh giiip cho sff khuech tan chat chidt tff ben trong te bao thoat ra dl dang hdn [7]. v l nguySn tac, neu tang dan lifdng dung moi so vdi cung mdt cd chat thi hidu qua trich ly se tang len do chenh lech gradient nong dp giffa ca'u tff can trich ly cua nguyen lieu:dung mdi tang len va ngifdc lai. Dieu dd lam han che sif khuech tan cua cac chat c^n trich ly vao dung mdi nen khdng the trich ly mdt each triet de cac cha't cd trong nguyen liSu. Dffa vao ket qua vl ham lu'dng saponin triterpenoid tdng, ti le nguyen lieu: nffdc la 1:30 se difdc sff dung cho cae khao sat ke tie'p.
3.2. Anh hudng cua thdi gian sieu dm de'n qud trinh trich ly
Khi tang thdi gian sieu am tff 5 phut de'n 20 phut thi ham Iffdng saponin triterpenoid tdng tang tff 1,7 lan den 2,2 lan so vdi mau khdng sieu am d cung dieu kien thdi gian; tuy vay, ne'u tie'p tuc tang thdi gian sieu am, hieu qua nay giam dang ke (Hinh 1).
Trong khi dd, d cac mlu cd sieu am, thdi gian sieu am 20 phtit cho ham Iffdng saponin tnterpenoid la cao nha't dat 1,65+0,2 mg/g, song ne'u tie'p tuc tang thdi gian sieu am thi ham Iffdng saponin triterpenoid tdng giam dang ke, tai nghiem thffc thdi gian 30 phut, ham Iffdng saponin triterpenoid tdng thu dffdc giam 30% so vdi nghiem thffc thdi gian 20 phut.
Theo ly thuye't, viec keo dai thdi gian lam gia tang cac tac ddng nen dan len cac te bao chat chie't va lam cho sff bie'n ddi cua nguyen lieu dien ra manh me hdn. Tuy nhien, thdi gian xff ly qua dai iam tang nhiet dd trong dung dich, dieu kien nhiet do cao, ham lu'dng saponin triterpenoid se giam do saponin de dang bi phan huy. Ngoai ra, thdi gian xff ly enzyme keo dai ciing lam tang lifdng hdp chat cao phan tff difdc giai phdng ra, tff do cd the lam tac nghen kenh din dich chie't trong Widi nguydn lieu, lam giam ham Iffdng saponin tnterpenoid tong thu dffdc [7]. Vi vay, chung tdi quyet dinh chon thdi gian 20 phiit de tie'n hanh cac khao sat tie'p theo.
Hinh 1: Anh hUdng cua thdi gian siSu dm den qud trinh trich ly saponin triterpenoid tdng
i\
Hinh 2: Anh hUdng cua cong sud't sieu am den qua trinh trich ly saponin triterpenoid long
150 1B7 5
11 III
225 2 6 2 5 300 i t Eieu am (W/g]
Am » M i u d5EChL/ng
3.3. Anh ht^ng cua thcfi gian sieu am de'n qud ttinh trich ly
Hinh 2 cho tha'y, khi tang cdng sua't sieu am, ham Iffdng saponin triterpenoid long tang d cac nghiem thffc cdng sua't tff 150 den 225 W/g. O cdng sua't 150 W/g, ham Iffdng saponin triterpenoid ia 1,22 mg/g; khi tang cdng sua't len de'n 225 W/g ham Iffdng saponin da tang 1,38 ian (1,65 mg/g nguyen heu). Sau dd, khi tang cdng sua't len de'n 262,5 W/g, ham Iffdng saponin triterpenoid cd chieu hffdng giam con 1,33 mg/g. Sff gia tang ham Iffdng cha't trich ly bang sdng sieu am cung tuan theo quy luat tffdng tff cua Jian - Bing va cgng sff (2006) da trinh bay d tren [7]. Tuy nhien, khi nang lu'dng sieu am
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TAP CHi CONG THtfdNB
qua cad, ddng nghia vdi nhiet do trong ldng dung mdi tang cao din den kha nang phan huy cua saponin triterpenoid cang cao.
3.4. To'i Uu hoa qud trinh trich ly dich chie't Id dinh ldng co khd ndng khdng en-zyme a - amylase cao nhat vdi sdho tr^cua song sieu dm
Phffdng phap be mat dap ffng difdc sff dung de xay dffng phffdng trinh hdi quy bieu diin sff anh hu'dng dong thdi cua 3 yeu td ti le nffdc:nguyen lieu, thdi gian sieu am (phut) va cdng sua't sieu am (W/g) den ham Iffdng saponin triterpenoid tdng (mg/g). Bd tri va ket qua thi nghiem dffdc trinh bay trong Bang 2 va Bang 3.
Md hinh dang toan phffdng bac hai theo ham lu'dng saponin triterpenoid difdc xac dinh bang hdi quy da bie'n, cd dang:
Y = bo + biX,+ b2.X-, + b3.X3 + b||-Xf + b22.X22-i- bjj.Xa^ + b|2.X|.X2 + b13.X1.X3 + b23.X2-X3
Vdi bo; b,; b-,; b,; b a ; b22; ^33; bij; b[3; b23 la he so cua bien X,; X2; X3; X^; Xj-; Xg^; X1.X2;
X1.X3; X2.X3 tu'dng ffng, X| la bie'n ma hda cua ti le nifdc:nguyen lieu, X2 la bie'n ma hda cua thdi gian sieu am, X3 la bie'n ma hda cua cdng suS't sieu am.
Trong 9 he sd hdi quy (trff bo) cd 3 he so hdi quy khdng cd y nghia vdi do tin cay P>0,05 la b]2, bi3, b23 chffng to sff tffdng tac giffa X|*X2, Xi*X3, X2*X3 la anh hffdng khdng dang kd' dd'n ham muc tieu. Vdi gia tri am, cac he so' hdi quy b2, b3, b] |, b22, b33 la sff tac ddng tieu cffc de'n ham muc tieu Y, lam giam gia tri. He so hdi quy cua b^ cdgia tri
Bang 2. Bo tri vd ket qua thi ngtilem toi lAi hoa
STT
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
Bi§h thuc Til$nu6c:
nguydn ii^u (x^) 25 35 25 35 25 35 35 25 35 25 30 30 30 30 30 30 30
Thdi gian siSu am (phut)(x2)
15 15 25 25 15 15 25 25 20 20 15 25 20 20 20 20 20
Cong suat sieu am (W/g)(X3)
187,5 187,5 187,5 187,5 262,5 262,5 262,6 262,5 225 225 225 225 187,5 262,5 225 225 225
Bleh ma hda
X1
-1 +1 -1 +1 1 +1 +1 1 +1 1 0 0 0 0 0 0 0
X2
+1 +1
+1 +1 0 0 1 +1 0 0 0 0 0
X3
+1 +1 +1 +1 0 0 0 0 -1 +1 0 0 0
Ham IUdng saponin triterpenoid tdng
0,798 ±0,013 0,988 ±0,035 0,713±0,013 0,922 ±0,021 0,687 ±0,010 0,855 ±0,022 0,578 ±0,008 0,755 ±0,011 1,387 ±0,031 1,552 ±0,026 1,579 ±0,008 1,404 ±0,011 1,501 ±0,012 1,318±0,014 1,652 ±0,023 1,661 ±0,018 1,656 ±0,028
HOA HOC-CdNG NGHE THIfC PHAM
Bang 3. Ke't q u a p h a n t i c h y nghTa c d c he so c u a p h u o n g trinh hoi q u y H0soh6iquy
bO bl b2 b3 b i l b22 b33 b12 b13 b23
Coen.SC 1,71737 0,0909 -0,0535 -0,0729 -0,29364 -0,27164 -0,35364 0,0035 -0,00675 -0,00725
Std. En.
0,027437 0,020276 0,020276 0,020276 0,039173 0,039173 0,039173 0,02267 0,02267 0,02267
P-value 6,98E-11 0,002856 0,033494 0,008794 0,000138 0,000224 4,18E-05 0,881657 0,774534 0,758446
Conf,lnt(±) 0,064878 0,047947 0,047947 0,047947 0,09263 0,09263 0,09263 0,053606 0,053606 0,053606
Hinh 3: Do thj bi mat ddp (fng (a, b, c) vd difdng dong mifc (d, e, f) the hien anh hifdng cua cac yeu to len ham IUdng saponin triterpenoid tdng thu du'dc (anh htfdng cua thdi gian vd cong sud't sieu dm (a vd d); anh hifdng cua cong sudt sieu dm vd ti le nifdc.nguyen lieu (b, e); anh hUdng cija ti le nifdc.nguyen lieu vd thdi gian sieu dm (c vd 0
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TAP CHi GONG THirUNG
dffdng Idn nha't, cho tha'y sff tac ddng ddn le cua Xi (ti le nguyen lidu: dung mdi) cd tac dgng tich cffc ldn den ham muc tieu (Bang 3). Anh hifdng cua cac ye'u to' len ham muc tieu la tac ddng theo phffdng trinh bac 2. Ngoai ra, gia tri R- = 0,989 va Q2 = 0,95 cua md hinh kha cao. Theo Gabrielsson va cgng su', md hinh thi nghiem dang tin cay la khi gia tri bie'n thien ao Q- >0,5 va R' >0,8; khi gia tri R- va Q- cang gan 1 thi ham hdi qui cang md ta td't cac ke't qua thi nghiem.
Bang 3 cung cho tha'y anh hu'dng cua ti le nguydn lidu: dung mdi (Xi), thdj gian sieu am (X2) va cdng sua't sieu am (X3) de'n ham lifdng saponin triterpenoid trong dich trich ly dffdc the hien qua phuTdng trinh hdi quy sau:
Y = 1,71737 + 0,0909.Xi - 0,0535.X2 - 0,0729.X3 - 0,29364.x 1 ^ - 0,27164.X2- -0,35364.X3^
Luc nay, ham lifdng saponin triterpenoid tdng dat cffc dai bang 1,73 mg/g, tai thdi gian sidu Sm la 20,75 phut, cdng suat sidu am 221,32 W/g, ti le mfdc: nguyen lieu la 29,50. Tid'n hanh thi nghiem kiem chffng tai dieu kidn td'i u\i, ke't qua ham lu'dng
saoponin triterpenoid tdng sd dat l,729±0,003 mg/g, chdnh lech khdng qua 5% so vdi ke't qua tdi ffu dff kid'n chffng td kd't qua td'i ffu dang tin cay. Gia tri nay cao xa'p xi 2,5 lan so vdi mau khdng dffdc xff ly sidu am d cung dieu kidn.
4. Kd't luan
Trong qua trinh trich ly vdi sif hd trd cua enzyme, cac ye'u to' khao sat nhff ti le nguydn lieu:nffdc, thdi gian va cdng suH't sieu 3m deu anh hffdng theo quy luat ham bac hai dd'n gia tri ham Iffdng saponin triterpenoid tdng thu dffdc. Ddng thdi, vide sff dung sidu am cd tac dung hd trd ro ret trong viec thu nhan saponin tnterpenoid tdng tff la dinh lang. Cac mau cd xff ly sieu am ludn cho ke't qua cao hdn han so vdi mau khdng xff ly sidu am.
Hdn nffa, qua trinh td'i ffu hda da xac dinh dffdc dieu kidn cdng nghd td'i ifu cho qua trinh trich ly saponin tnterpenoid tdng tff la dinh lang la thdi gian sidu am 20,75 phut, cdng sua't sieu am 22l,32W/g va ti Id nffdc:nguyen lieu la 29,5 (v/w), tai dieu kidn td'i ifU nay, ham Iffdng saponin triterpenoid tdng dat 1,73 mg/g nguydn lieu, cao xa'p xi 2,5 lan so vdi mau khdng dffdc xff ly enzyme •
LM cdm On:
Nghien cdu ndy do Tri0ng Dgi hoc Cdng nghiep ThUc phdm Thdnh pho'Ho Chi Minh bdo trtf vd cd'p kinh phi theo Hc/p dong sd60/HD-DCT.
TAI LIEU THAM KHAO:
1. Vd Vdn Chi (2004). TU dien ThUc vat thong dung. lap 1. NXB Khoa hoc vd Ky thuat
2. Cdy thudc vd dong vdt ldm Ihudc d Viet Nam, lap 1. NXB Khoa hoc vd Ky thudt; dinh ldng trang 793 - 796.
3. Ngd Ifng Long (1985). So sdnh tdc dung tdng luc vd .sinh thich nghi cua dinh ldng Polysciasfruticosa L. Harms, Chdnchim vdEleuterococ. TapchiDuoclieu, lap2sol,24-27.
4. Do Huy Bich, Dang Quang Chung, Biii Xudn Chuang, Nguyin Thuang Dong, Dd Trung Ddm, Pham Vdn Hien, VU Ngoc Lo. Pham Duy Mai, Pham Kim Mdn, Dodn Thi Nhu, Nguyin Tdp, Trdn Todn. Vien Duac lieu (2004). Cdy thudc vd dgng vdt ldm thudc. tdp 1, NXB Khoa hoc vd Ky thudt Hd Noi.
5. Somova L. O.. et al.. (2003). Cardiovascular, antihyperlipidloiic and antio.Kidani effects ofoleanolic and ursolic acids in experimental hypertension Phytomedicine, 10(2-3), 115-121.
6 Dong Huaihai, Gu Wenying (200!) Determination of .soybean saponins using colorimetry, China Oils and Fats 26 (3), 57-59.
7. Jian-bing Ji. Xiang-hong Lu, Mei-qiang Cai, Zhi-chao Xu (2006). Improvement of leaching process ofGeniposide with ultrasound. Ultrasonics Sonochemistr)', 13(5), 455 - 462
HOA HOC-CiilllG NGHE TfltfC PHAM
Ngay nhSn bai: 18/11/2019
Ngay phan bien danb gia va su'a chifa: 28/11/2019 Ngay cha'p nh$n dang bai: 9/12/2019
Thong tin tdc gid:
ThS. HA THI THANH NGA TRlfONG BAO NGHI B 6 HONG PHlTONG THAO ThS. TRAN CHi HAI Khoa Cong ngh§ thtfc pham
TrirBng Dai hoc Cong nghiep ThiJc pham thanh ph6'H6 Chi IVIinh
ULTRASOUND - ASSISTED EXTRACTION THE TOTAL SAPONIN TRITERPENOID FROM THE POLYSCIAS FRUTICOSA (L.) HARMS LEAVES
• MA. HA THI THANH NGA
• TRUONG BAO NGHI
• DO HONG PHUONG THAO
• MA. TRAN CHI HAI Faculty of Food Science and Technology Ho Chi IVIinh City University of Food industry
ABSTRACT:
In this study, the effects of the raw materials: water ratio, ultrasonic power and ultrasound time on the total saponin triterpenoid content extracted from Polyscias fruticosa (L) Harms leaves were investigated. The results showed that all three factors had an effect on the total saponin triterpenoid content obtained by the quadratic function rule. By response surface method, the optimum parameters have obtained such as ultrasonic time of 20.75 minutes, ultrasonic power of 221,32 W/g and the ratio of water per raw material of 29.50. At these optimum conditions, the total saponin triterpenoid content was approximately 2,5 time higher than that of samples without ultrasound treatment.
Keyword: Polysciasfruticosa (L.) Harms leaves, ultrasonic power, ultrasonic time, the total saponin triterpenoid content, the ratio of raw material per water.
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