KHOA HOC C O N G N G H l
KET QUA CHDIM TAO GIOIVG LAC M O I D M 1 KHAIXIG BEIXIH D O M LA MLIOIV
Nguyen Tlu Thiiy Ngoan^ Phan Thanh Phuong^ Nguyen Thanh LoanS Nguygn Ehic CuongS Ddng TTii Kim Ctic^
TOMTAT
Gidng lac DM1 do Vien Di Uuyen Nong nghiep chon IQC tir dong CLl, la k^t qua tir to hop lai giua giong lac CNC3 vol giong TN6 bang phmmg phap truyen thong ket hop voi cong nghe sinh hpc, la gidng lac tnen vpng CO nhieu dac diem nong hoc tot. Ket qua khao nghiem DUS cho thay gidng co tinh khac bi^t, tinh dong nhat va tinh on dinh. Ket qua khao nghiem VCU tai cac tinh phia Bac cho thay giong lac DM1 co then gian smh tiwng 120 - 125 ngay trong vu xuan va 95 - 105 ngay trong vu thu dong tuong dirong giong ddi chiing L14. Gidng lac DM1 co cac yeiu td cau thanh nang suat va nSng suat vuot gidng ddi chung v^ mot sd chi tieu nhu sd hat/cay, khdi luong 100 qua, khdi lugng 100 hat. Nang suat trong khao nghiem ca ban trung binh cac diem dat 2,63 - 3,94 tan/ha vuot gidng ddi chiing L14 tir 10,3 - 18,1%, trong khao nghiem san xuat vuQt gidng ddi chimg L14 tir 11.3 - 23,8%. Gidng lac DM1 chdng chiu tdt ddi voi cac loai sau benh hai chinh nhu: Heo xanh, thdi qua (diem 1), nhi^m nh^ gi sat, ddm nau. Dac biet gidng lac DM1 khang dupc benh ddm la muon - ddm den (diem 0 - 1) da dupc danh gia nhd chi thi phan tu va tren thuc ti&n ngoai ddng ruong
Tirkhda: Gidng lac DM1, bSnh ddm la mudn.
I.DATVAniOE
Benh ddm la muon {Phaeolsariopsis personata) la mot trong nhung b^nh la gSy hai nghiem trong dot voi cay lac. B6nh co the tan cdng tat ca cac bo phan phia trfin mat dat cua cay lac nhu la, canh, than, hoa nhung la U noi chiu thiet hai nang ne nhat lam giam nang suat tir 10 - 50%. Dieu kien thai tiet nong am a mien Bdc nuoc ta rat thich hop cho benh ddm la muon phat trien va gay hai b lac. Vi^c tao ra gidng lac CO d§c tinh khang b6nh ddm la muon se gop phan quan trong lam 6n dinh nang suat va chat luong lac khi gap di^u kien bat thuan. Nhom tac gia Vien Di truyen Nong nghiep da chon tao duoc gidng lac khang h6nh ddm la muon DM1 nhcr chi thi phan tii, budc dau danh gia la co kha nang sinh trudng phat trien tdt, nang suat cao va khang dupc b^nh ddm la muon. Gidng duoc dua vao he thdng khao nghiem Quoc gia nham danh gia kha n^ng sinh truong phat trien b cac tieu vimg sinh thai, de tidn toi cong nhan gidng chinh thiic.
2. VAT UEU VA PHUONG PHAP lUGHIBU CUU 2.1. V$t lieu
- Gidng lac DM1 do nhom tac gia ciia Vien Di truyen Nong nghiep chon tao tir to hop lai CNC3 x
TNG bing phuong phap lai hiiu tinh ket hgp voi su dung chi thi phan tu.
- Gidng ddi chung: gidng lac L14.
- Cac chi thi phan tu hen ket gen khang benh ddm la muon (PM179; GM633; GM2301; IPAHM103;
L e d ; seq7G02; TC9F10 va GM1760).
- Sir dung nguon vi khuan gay benh ddm la muon do Vien Bao ve Thuc vat cung cap.
2.2. Phuong phdp
2.2.1. Pbuongphap chon tao gidng - Lai hiru tinh va chon loc ca the.
+ Khu due chieu ngay hom truoc khi lai (cay nhan gen CNC3).
+ Lay phan cay cho gen CTNG) thu cho cay nhan gen da dugc khu due vao liic 8 -10 h sang hom sau.
- Phirong phap chi thi phan ttr (tach chiet ADN;
phuong phap PCR; phuong phap di^n di tren gel Agarose...).
- Phuong phap lay benh nhan tao va danh gia benh ddm la muon.
- Phuong phap thi nghiem ddng ruong 2.2.2. Khao nghiem tac gia
- Thi nghiem dupc bd tri theo khdi ngSu nhien day dii. 3 lan nhac lai. dien tich mdi 6 thi nghiem la 10m-.
Vien Di truyfin Ndng nghiep
61
KHOA HOC G O N G NGHE
- Quy trinh canh tec va danh gia dua theo "Quy chuan ky thuat Quoc gia v i khao nghi6m gia tn canh tac va su dung ctia giong lac" (QCVN 01- 57:20U/BNNPTNT).
- Doi chiing: Gi6ng lac U 4 (Nguon goc lay tir Tmng tam Nghien ciiu & Phat Wen Dau d6 - Vien Cay luong thirc & Cay thuc phSm)
2.2.3 Khao nghidm DUS
Thi nghiem duoc thuc hien theo quy pham khao nghidm tinh khac biet. tinh dong nhat va tinh on dinh cua giong lac QCVN01-67:2011/BNNPTNT do Bp Nong nghiep va PTNT ban hanh.
2.2.4 Khao nghiem VCU
Khao nghidm gia tri canh tac va sir dung (khao nghiem VCU) duoc thuc hien theo Quy pham khao nghiem gia tri canh tac va gia tri str dung cua giong
Gi6nEl,acCNC3-<BtP^ I GiSng Lac TN6
i
lac QCVN01-57:2011/ BNNPTNT do Bp Nong Nghiep va PTNT ban hanh.
2.2.5. Khao nghiem san xuat
Thi nghiem dupc bo tri dien tich toi thieu 500 mVdiem. ap dung quy trinh den ti& nhat va thoi vu thich hcfl) cua timg dia phuong noi lam thi nghiem.
danh gia sinh trucmg phat tnen, miic do nhiSm sau benh hai va kha nang diich ling ciia giong tai dia phuong. Doi chiing so voi giong dang dupc trong pho bien tai dia phuong la L14.
2.3. Phuong phip phan tich s^ !i6u
Sd lieu dupc xu ly thdng ke theo chuong trinh Excel va IRRISTAT.
3.l(ETQUANGHraCIIU
3.1. Qui trinh chpn tao gidng Uic DM1
Vu Xuan 2011 Phenotyping.
- Genotyping
- Phenotyping.
- Genotyping
BC2F1 - Phenotyping.
- G e n o t ^ i n g .
- Lap ban do
R)
- Khao n ^ i e m tac gia.
- Khao n ^ i e m quoc gia, - Khao nghiem san x u i t
] ®
BC2F3
BC2F4
- Xuan. Thu Dong 2014 -Xuan2015:2016: 2018;
ThuD6ng2018
- Tii "IhuDong2015-Xuan 2019
Hinh L So d6 chon t^o gidng lac DM1 khing b6nh ddm l i muOn bang chi thj phSn tit Ghi chti- RP: Recipient parent (giong nhan gen): DP: donor parent (gidng cho gen khang).
3.1.1. Banh gia kha nang khang benh ddm la mudn aia gidng tham gia nghien cau
De cung cap nguon vat lieu khdi dau cho viSc tao con lai CO kha nang khang benh ddm muon. tien
hanh danh g i i tinh khing benh cua tap dpan gipng ^ ^ „ g ' „ h a n gen'cho c i c thi nghiem hep theo lac thu thap dupc. Ket qua danh gia tinh khing benh ^ ^ ^ ^^^^^ ^/^ ^^^^ ^,^^ ^^^ .j-j^g ^ ddm muon ciia tap doin 64 gidng lac thu thap dupc
cho thay giong CNC3 hi nhiSm benh nang nhat. ty le
benh til 11,22%; chi sd benh tii 1.55%. Gidng TN6 nhiSm benh rat nhe, ty le benh 1,62%; chi sd benh 0.15%. Gidng lac CNC3 co kha ning nang suat cao (>
200 g/cay) va gidng TN6 nang suat thap (< 150 g/cay). Nhu vay gidng CNC3 se duoc chon lam
KHOA HOC CdNG NGHE Bang 1. Kha nang k h ^ bfinh ddm muOn cua gidng
lacCNC3v^TN6
{Ruong thinghi$m tai An Khanh vu xuan 2012^
Ten giong CNC3
A. cardenasii (TN6) Ty le benh
i%)
11,75 1,62
Chi s(
benh
(%)
1,67 0,15
Kha nSng nang suat (g/cay)
>200
<150 3.1.2. Su dung chi thi phan tu USn kdt QTL khang benh ddm muon trong chon gidng
Vm thi nghiem nay, da tao ra the he lai Fl, sau do sii dung chi thi hen ket voi gen khang de xac dinh cac ca the mang gen b cac the he Fl, BCIFI, ...
dong thoi ket hpp lay nhiim nhan tao va danh gia ti-en dong ruong d^ chpn dupc cac dong/giong uu viet nhat de nhan rpng trong san xuat.
*Lai tao va chon loc cac quin the gidng lac khang benh ddm la muon, nang suit cao Fl, BCIFI, BC2F1,...
- Ket qua da lai tao dupc 18 hat F,. Cac ca th^ ff the he F l dup'c dua vao danh gia kiem tra xac dinh mang gen khang b^nh dfi'm la muon de tiep tuc phat trien cac the he tiep theo.
- Tiep tuc sang loc cac con lai a cac th^ he BCIFI; BC2Fl...mang gen khang benh dom la muon dong thai cung danh gia cac dac tinh n6ng sinh hoc va n^ng suat de chpn loc cac ca the uu hi de phat trien ra cac th^ he ti^p theo.
- Trong 16 ca th^ BCiF, lai tao dupc co 11 ca th^
CO tinh khang cao voi benh dom la muon se dupc chpn de tao cac the h^ ti^p theo,
- Va 11 ca the BC,Fi c6 tinh khang cao nay se dupc lai tra lai vdi dong me CNC3 de tao ra cac ca the BC^F, CO nang suat cao, khang benh dom la muon. Ket qua tao dupc 48 ca the BCaF,. Cac ca the nay se dupc kiem tra de xac djnh gen dom la muon bang cac chi thi hen ket. Mot so hinh anh minh hpa.
Hinh 2. Sii dung chi thi TC9F10 dg chpn ck th^ mang gen khang th^ h$ BCaFi v6i A. CNC3; B. TN6; H. con lai dupc chpn
Hinh 3. Sir dung chi thi IPAHM103 d^ chpn ca th^ mang gen khteg th^ he BC2F1 vdi A. CNC3; B. TN6; H.
con lai dupc chpn
Hinh 4. Su dung chi thi seq7G02 d^ chpn ck th^ mang gen khang th^ h$ BC2F1 vdi A. CNC3; B. TNB; H. con lai dupc chpn
Voi 29 ca the b the he BC^F,, da phat trien dupc Su dung phuong phap chi thi phan hi hen ket
QTL/gen khang benh ddm la muon chiing toi da xac dmh dupc 29 ca the d the he BC.Fi mang QTL lien ket gene khang. Cac ca the nay dupc giu lai de tiep tuc chon loc trong the he tiep theo.
24 dong d the he BC.F. (tir CLl den CL24). Cac ddng nay se duoc sang loc bang cac chi thi phan tu lien ket chat vdi gene khang benh ddm la mudn. Ket qua dai dien duoc the hien d cac hinh 5 va 6.
63
KHOA HOC CONG NGHfi
Hinh 5. Sii dijng chi thi PM179 ii chon c i M mang gen khing ddng CLl vdi A. CNC3; cic c i thS B se dupc chpn trong th^ hd t i ^ theo
A A B B A B A A B A B B B
Hmh 6. Sa dung chi thi PM179 d^ chpn c i thi mang gen khing ddng CL5 vdi CNC3; cic c i thS B se dupc chpn trong th^ he tidp theo
Tong hpp cac nghien ciiu vd tinh trang ndng Trong dd ddng DM1 dupc dua khao nghiem tac sinh hpc, nang suat. tinh khang benh cua cac ddng gia o thd he BC2F3, trong vu xuan 2014 va thd he lac BCjFj d tren. ket qua chpn dupc 6 dong BC^Fj cd BC2F4 trong vu thu ddng 2014 tai An Khanh, Hoai nang suat cao hon ddi chiing CNC3 va co tinh khang Biic. Ha Ndi.
hoac khang cao vdi benh ddm la mudn. ddng thdi 32. Khao nghito tic gia khdng man cam vdi mdt sd bdnh hay gap khac tren
cay lac. dd la cic ddng:' CL,. CL,. CL3.CL4. CL,. CL,. f^-^- *^''" ^ ^ ^ "^"^ » " * *<^ """ ^""^ '^'^
CL,2 va dupc d a ten theo thii ttr la DM1; DM2;
DM3; DM4; DM5; DM7; DM12.
STT 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Bing 2. MOt sd d$c di^m ndng sinh hpc cua gidng lac DM1 (vi^ xuin 2014) -—._______^ Gidng lac
Dac diem
Thdi gian sinh trudng (ngay) Chieu cao cay (cm) Sd canh cip 1 Sd qua chac/cay
H a t Man vd hat chin (hat tuoi) Hat: Dang hat
Hat: Khdi lupng 100 hat (gam) Hat: Thdi gian ngu nghi Tyle hat/qua (%)
Muc dd nhiem benh gi sit (didm) Miic dd nhiem benh ddm niu (diem) Miic dd nhiem ddm den (diem) Miic dd nhidm benh heo xanh (%) Nang suat tidm ning (tan/ha)
L14 (DC) 118 ± 3 39,3 ± 2.2
4.5 ± 2.5 10.5 ± 3,2
Hdng Bau due 64.8 ± 5.5
Ngan 71.5 1-3 1-3 1-3 1.1 2.94-2.99
DM1 116 ±4 43.4 + 3.5
5.4 + 2.1 17.8 ± 3 1 Trdng hdng
Biu due 80.98 ±4.8
Ngan 87.2 1-3 1-3 0-1 0.7 3.61-3.85 Vd kidu hinh. gidng DM1 cd kidu hinh dang ciy Giong lac DM1 co chidu cao dao ddng ti, 4347 cm, nila dung, vd lua hat miu tring hdng. cd thdi gian ^ h ngu nghi cua hat ngan^ So qua 2 nhan chiem ty sinh birtag trong vu xuan nam 2014 la 116-120 ngiy. '* " ° ™ '^^^ ^ ^ ^ «»" "hu khdng co. Qua ^^^ \
KHOA HOC C 6 N G NGHt
cho th^y, gidng lac DM1 cd kha nang chdng chiu sau benh hai va dieu kita sau benh tdt nhidm rat nhe gi sat, ddm den (diem 1 -3); heo xanh, (didm 1). Dac bift. gidng lac DM1 khing benh ddm den (diem 0-1) ttong khi ddi chiing L14 (diem 1- 3).
3.2.2. Cac ySu to can thanh nang suat vi nang suat cOa gidng lac DM1
Ket qua theo ddi cic ydu td cau thanh NS va nang suat gidng lac DM1 duoc trinh biy d bang 3.
Ten gidng
L14 DM1 CV(%) ICDo.05
Bi i n g S . C i Sd qua c h i c / c i y
Thu ddng
8.8»
13.4"
7,1 2,3
Xuin 13,8=
17.4' 8,3 2,5
c y & t d c i u thinh nanj PlOO qua (g) Thu ddng
165'' 175"
2,2 5,2
Xuin 171.5' 183.5' 2,5 8,3
^ s u i t v i ning suit PlOO hat fe) Thu ddng
61"' 67"
3,4 6,0
Xuan 63»' 69- 3,1 6,3
cdagidnj g lac DM1 Nang suat TT
(tan /ha) Thu ddng 2.75' 3.37"
6,7 3,2
Xuan 3,24' 3,85"
6,1 2,7
Trung binh 2.99 3.61
%Vuot d/c
20.7
Ket qua dbang 3 cho thiy:
Sd qua chic/cay ciia gidng lac DM1 dat tir 13.4 - 17,4 qui cao hon ddi chiing L14 (8,8 -13.8 qua) tit 26 - 52.3% qua d miic cd y nghia va cao hon cac gidng con lai trong thi nghiem song giira chung khdng sai khac cd y nghia thdng ke d dp tin cay 95%.
Khdi luong 100 qua cua gidng lac DM1 dat tir 175.0- 183.5g cao hon ddi chiing L14 (165 -171,5 g) tit 4,8 - 7,0% qua d miic cd y nghia,
Khdi lupng 100 hat cua gidng lac DM1 dat tir 67 - 69 g cao hon ddi chiing L14 (61 - 63 g) tu 9,5 - 9,8% g 6 miic sai khac khdng cd y nghia.
Do cd su sai khac b ttrng yen td cau thanh nang suat nhu sd qua chac/cay va khdi luong 100 qua da din ddn nang suat thuc thu cua gidng lac DM1 so vdi gidng ddi chiing L14 sai khac nhau co y nghia. Cu the. nang suit thuc thu cua DM1 dao ddng tu 3.85 - 3,37 tan/ha cao hon so vdi ddi chung L14 20,7%.
3.3. Khao nghiem DUS
Tinh khac biet Gidng DM1 khac biet so vdd gidng L26 phd bien. Cd 3 su khac biet ro duoc trinh bay trpng bang 4.
Tinh dong nhat: Sd cay khac dang trdn tdng sd cay quan s i t la: 1/200 khdng vupt qua sd cay khac dang tdi da cho phep (3/200 cay) ndn gidng dang ky cd tinh ddng nhat.
Tinh on dinh: Gidng da qua hai vu khao nghiem nen ket qua dinh gii gidng cd tinh dn dinh.
Bang 4. Tinh trang DM1 so vdi gidng Tinh trang
7 I S O
20
Li chet Man sac Hat Khdi luong 100 hat (o do am
hat 9.0%) Hat Tyle nhan/qua
Gidng dang kv
7 75,00
7L15 Gidng tuong hi 5 78,33
74.78
tuong tuL26 Khoing each
tdi thieu/LSDfto-i
2 3.79
1.86
3.4. Kdt q u i khio nghi$m s i n xuit gidng l^c DM1
Vdi gidng lac tnen vpng DM1, song song vdi viec khao nghiem Qudc gia, trong thdi gian tir 2016 - 2019 da tidn hanh khao nghiem san xuat (7 vu) tai mdt sd vung sinh thai khac nhau nham thu kha nang thich ling cua cic gidng lac mdi. Da tridn khai khao nghiem san xuat tai 9 diem: Hiep Hda. Bic Giang (4.2 ha); Dudng Lam, Son Tay, Ha Ndi (4.2 ha). Lac Thuy. Hda Binh (3.5 ha); Tir Liem. Ba Vi. H i Ndi (6.65 ha); Quynh Phu. Thai Binh (1 ha); Tho Xuan, Thanh Hda (1,5 ha); Didn Chau, Nghi Ldc, Nghe An (27,2 ha).
Ket qua d bang 5 cho thay; Tai vimg trung du mien nui phia Bic gidng lac DM1 cd thdi gian sinh trudng trong vu xuan dao ddng tir 120 - 125 ngiy.
tuong duong vdi gidng lac ddi chiing L14. Nang suat thuc thu dat tir 3.60 - 3.66 tan/ha vupt gidng ddi chung L14 tir 15.3-21.2%.
65
KHOA HOC CdNG N G H £
- Tai vung ddng bSng sdng Hdng. thdi gian sinh trudng cua gidng lac DM1 trong vu xuin dao ddng khoang 122 - 127 ngay. (hrong duong gidng lac ddi chimg L14). Nang suat thuc thu a cic diem dat td 3.37 - 3,9 tan/ha vupt gidng ddi chiing L14 hi 11.3 - 19,9%.
- Gidng lac DM1 dupc khao nghidm san xuat 2 tinh Thanh Hda va Nghe An thudc khu vuc Bic Trung bd. Ket qua cho thay thdi gian smh dudng cua gidng lac DM1 frong vu xuan dao ddng tir 115 -125 ngiy. (tuong duong gidng lac L14. Nang suit thuc thu dat til 3.53 - 3.90 tan/ha vupt ddi chiing L14 tir 14.7-23.8%.
angS.Thdigiar Vung sinh
thai Trung du
midn nui phia Bic
Ddng bang sdng Hdng
Bic Trung bd
1 smh hTidng & ning suit cua gidng lac DM4 frong c i c vu TiiSn 2016.2017.2018 v i 20 Dia diem
Hda Binh Bac Giang TirLiem. HaNdi Son Tiy. H i Ndi BaVi.HaNpi
Thii Binh
Thanh Hda Dien Hoa. Didn Chau. Nghe An Vien KHKTNN Bac Trung bd
(Nghe An) Didn Thinh. Didn Chau & Nghi Ldc.
NghdAn
Ten gidng D M 1 L14 D M 1 L14 D M 1
L14 D M 1
L14 D M 1
L14 D M 1
L14 D M 1
L14 D M 1
L14 D M 1
L14 D M 1
L14
TGST (ngay) 125 124 120 120 126 125 122 122 125 125 127 126 118 118 120 122 125 125 115 115
Nang suat TT (tan/ha)
3.60 3.12 3,66 3,02 3,37 2,81 3,9 3,30 3,82 3,31 3,65 3,28 3,53 2,85 3,82 3,29 3,80
3,90 3,40
%vuot d/cL14 15,3
21,2 19,9 18,12 15,2 11.3
23.8
16.1
14,7
Nguon: Tdng hop bao cao tai cac dia phirong tham gia san xuat Gidng lac DDMl cung da dupc khao nghiem
trong cac vu thu ddng 2015. 2017. 2019 ket qua dupc the hien frong bang 6.
Ket qua d bang 6 cho thiy:
- Tai vung trung du midn nui phia Bic gidng lac DM1 cd thdi gian sinh tnidng frong vu thu ddng la 95 ngiy. hrong duong vdi gidng lac ddi chiing L14.
Nang suat thuc thu dat 3,22 ta/ha vupt gidng ddi chung L14 14,2%.
- Tai viing ddng bang sdng Hdng. thdi gian sinh trudng cua gidng lac DM1 frong vu thu ddng dao
ddng hi 95 - 105 ngay tirong duong gidng lac ddi chung L14. Nang suit thuc thu d cac diem dat tir 3 27 - 3.40 tan/ha vupt gidng ddi. chiing LU tir 15 2 - 17.2%
- Gidng lac DM1 dupc khao nghiem san xuit tai 2 tinh Thanh Hda va Nghe An. Ket qua cho thay thdi gian sinh frudng cua gidng lac DMl frong vu thu ddng dao ddng tir 90 - 105 ngay hrong duong gidng lac L14. Nang suit thuc thu dat tir 3,0 - 3 3 tin/i, vupt ddi chiing L14 tir 15,4 - 19,5%. ' "
KHOA HOC CONG N G H l
Bang6.Th<«
Vimg smh thai Trung du mien nui phta Bac Ddng b i n g sdng Hdng
Bac Trung bd
gian stah trudng v i ning suit cda eidne lac DMl trong vu thu ddng 2015.2017,2018 Dia didm
Bic Giang
Son Tay, HaNdi BaVi.HaNpi Didn Hoa, Didn Chau, Nghe An Vien KHKTNN Bic Trung bd (Nghe An) Didn Thinh Didn Chau & Nghi Ldc, Nghe An
Ten gidng D M l L14 D M l
L14 D M l L14 D M l L14 D M l L14 D M l L14
TGST (ngiy)
95 95 105 100 95 95 90 92 100 100 105 105
Nang suit TT (ta/ha)
3,22 2,82 3,40 2,90 3,27 2,84 3,00 2.51
3.30 2.90
%vupt d/c L14
14,2
17,2 15,2 19.5
15.4
9 cao tai cac dia phtrong tham gia san xuat Nguon: Tdng hop I
4.KETUIANgENGHI 4.L Kdt luin
- Ket qua chpn loc va khao nghiem tic gia tir nam 2014 den nam 2015 cho thay: Gidng lac DMl cd ki^u hinh dang cay nua diing. phan canh lidn tuc.
than mau xanh. l i xanh dam. eo qua ndng. vd qua day trung binh, bd mat cd gan q u i rd, hat bau due, vd lua frang hdng. Gidng cd cac yeu td cau thanh nang suat va nang suit tuong duong gidng ddi chung va vupt mdt sd chi deu ve khdi lupng 100 qua. khdi luong 100 h a t Hem nang nang suat cao.
- Gidng lac DMl cd tinh khac biet tinh ddng nhat va tinh dn dinh.
- Gidng lac DMl cd thdi gian sinh frudng frong vu xuan tir 115-127 ngay. vu thu ddng tir 90-105 ngay.
nang suat dat 3.4 - 3.9 t i n / h a frong vu xuan. vupt gidng ddi chung L14 tii 11.3 - 23.8% va 3,0 - 3,4 tan/ha frong vu thu ddng vupt ddi chung tir 14,2 - 19,5%.
- Gidng lac D M l chong chiu tdt ddi vdi cac loai sau benh hai chinh nhu: Heo xanh, thdi den cd rd, ttidi frang than, thdi qua (diem 1), nhiem nhe gi sat.
ddm nau. Dac biet gidng lac DMl khang dupc benh ddm la mudn - ddm den (diem 0 -1) da duoc danh gia nhd chi thi phan hi va fren thuc tidn ngoai dong mpng.
4.2. Dd nghi
- Ti6n hanh nghien cuu cac bien phap ky thuat nham tang nang suat va hieu qua kinh td frong viec san xuat gidng lac DMl.
Cdn md rpng dien tich gidng lac DMl co nang suat cao, chong chiu sau benh hai de tang hi^u qua cua viec san xuat lac.
TAI UEU THAM KHAO
1. Bp Ndng nghiep va PTNT (2011). Quy chuan ky thuat Qudc gia ve khao nghiem gia tri canh tac va sir dung cua gidng lac, QCVN 01-57;
2011/BNNPTNT ban hanh tai Thdng tu sd 48/2011/Tr-BNNPTNT ngay 5 thang 7 nam 2011.
2. Dong Thi Kim Ciic, Le Thanh Nhuan, Phan Thanh Phuong, Luu Minh Ciic, Ha Mmh Thanh, Nguyen Van Quang, Pham Thi Mai, Nguyen Thanh Loan va cs (2016). Nghien cuu chpn tao gidng lac khang benh ddm la muon bing chi thi phan tii. Bao cao tong ket.
3. D6ng Thj Kim Ciic, Luu Minh Ciic, Le Thanh Nhuan, Ha Minh Thanh, Phan Thanh Phuong, 2016.
Ung dung chi thi phan tii ti-ong chon tao gidng lac khang benh ddm la muon. Hoi thao Quoc gia khoa hpc cay trdng lan thu II. The second national conference on crop sciences, tr916 - 923.
4. Luu Minh Cue, Luu Thi Ngpc Huyen, Ddng Thi Kim Ciic va cs (2013). Nghien cuu da dang di truyen cac gidng lac bdng chi thi phan tii. Tap chi KH&CN NNVN, so 2(41). Tr 38 - 43.
5. Nguyen Van Thdng (1995). Danh gia nguon vat heu khang benh ddm den cua cay lac trong diiu kien mien Bdc Viit Nam. Luan an thac sy khoa hpc nong nghiep. Ha Npi. 75 tr.
67
KHOA HOC CONG N G H l 6. Nguyen Xuan Hdng (1994). Bdnh hai lac a
Viet Nam v i chien luoc nghien ciiu. phdng tra.
Tuyen tap cac nghien am khoa hpc cong nghe. Vien Khoa hpc Ky thuat Ndng nghlip Viet Nam, Ha Ndi fr. 4S50.
7. Tran Dmh Long va Nguydn Thi Chinh (2005).
Ket qua chpn tao va phit frien gidng dau dd 1985- 2005 va dinh hudng phat fridn 200^2010. Bao cao tong kd't 20 nam ddi mdi va dinh hirdng nghien ctiu mai doan 200&2010. Hdi nghi Khoa hpc Chuydn nganh Trdng frpt ngay 10-11 thing 3 nam 2003.
Trung tam Nghien ciiu va Phat hien Dau dd. 19 frang.
8. Tran Dinh Long (2005). Bao cio tdng ket Khoa hpc vi ky thuat du an "Hoan thien quy trinh san xuat mdt sd gidng lac mdi L02, LOS, L14, MD7, MD9 phuc vu san xuat lac xuat khau va ti&u dung tiong nirdd\ Bd Khoa hpc & Cdng nghe. Vien Khoa hpc Ky thuat Ndng nghiep Viet Nam. Trung tim Nghien ciiu Thuc nghiem Dau dd. 49 fr.
9. Biology. 2003. vol. 26. no. 1. p. 77-82. Beavis.
William D. QTL analyses: power, precision and accuracy. In: PATERSON. A.H. ed. Molecular dissection of complex traits. CRC Press, Boca Raton.
1998. p. 145-162.
10. Benbouza. Halima; Jacquemin; Baudoin.
Jean-Pierre and Mergeal, Guy (2006). Optimization of a reliable, fast cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels.Biotechnology, Agronomy, Society and Environment, 2006. vol. 10. no. 2. p. 77-81.
11. Cue. Luu M.. MACE, Emma S , Crouch.
Jonathan H.. Quang. Vu D., Long. Tran D. and Varshney, Rajeev K (2008). Isolation and characterization of novel MlcrosatelMte markers and their appUcation for diversity assessment m cultivated groundnut {Arachis hypogea L ) . BMC Plant Biology, May 2008, vol. 8, no. 55.
12. Dong Thi Kim Cue. Ha Minh Thanh, L T.
Nhuan. Le Thu Hien. Tran Thi Loan. Phan Thanh Phuong and T. D. Khanh (2015). Research peanut hreedmg for disease resistance by spotting late marker. Intemational Joumal of Development Reaserch Vol. 5. Issue. 12. pp. 6125-6129.
13. Tiwari S. P . Ghewande M. P. and Misra D. R (1984). Inhemtance of resistance tp mst and late leaf spot in groundnut (Arachis hypogaea). Joumal of Cytology and Genetics 19. pp. 97-101.
14. Food and Agriculture Organization of the United Nations (2017). hBp://faostatfao.org/
RESULT OF SELECTING NEW PEANUT DMl RESISTANCE TO LEAF DISEASE LATO Nguyen Thi Tliuy Ngoan', Phan Thanh Phuong*, Nguyen Thanh Lpan^ Nguyen Due CuongS Dong Thi Kim Cue' 'Agrlculhiral Genetics Institute Summary
DMl peanut variety selected by the Agricultural Genetics Institute from CLl, CNC3 x TN6 liybrid combination by fraditiona! methods combined with biotechnology, is a promising peanut variety with many good agronomic characteristics. DUS test results DMl peanut variety have distinctness, uniformity, and stability. VCU test DMl has a growtii time of 120 - 125 days in die spring crop and 95 - 105 days in the autumn-winter crop equivalent to the L14 confrol vanety. The peanut frees are semi-slanding type continuous branching, green stems, dark green leaves, shallow waist, medium thick pods, clear nbbed surface, oval seeds, pink-white silk bark. The vanety has yield components and the yield exceeds that of die confrol varieties, a number of criteria on Oie number of seeds/ tree, the weight of 100 fruits and die weight of 100 seeds. The average yield in the field tnals averaged 2.63-3.93 ton/ha in excess of the U4 confrol vanety from 10.3 -18.1%. in the frial of production exceeded L14 confrol variety from 11.3- 23.8%. Spencial DMl peanut varieties resistance to late leaf spot disease (0-1 mark).
Keywords: Peanut varieties DMl, late leaf spot disease.
Ngudi phin bi$n: GS.TSKH.VS. Trin Dinh Long Ngiy nhUn bii: 13/3/2020
Ngiy thdng qua phin bi$n: 14/4/2020 Ngiy duy$t ding: 21/4/2020
