KHOA HQC KY THUAT THU Y TAP XIX SO 2 - 2012
PHAN LAP MOT SO VI KHUAN CONG PHAT GAY BENH
«.'6 LON NGHI
M A C BENH S U Y I N ,DE XUAT BIEN PHAP PHONG TRI BENH
Li Vdn Lanh', Huynh ThiM^Lf', Trinh Dinh Thau', Bdng HOtu Anh', D§NgQC Thdy^ vd Nguyin Bd Hiin'
T6M TAT
Phan l^p vi khuan kl phdt d Ipn nghi mdc bfnh suyen. kilm tra khd ndng min cdm khdng sinh dl xdy dung bifn phdp phdng trj bfnh Id myc tieu va nfi dung chinh ciia nghien ciai nay. Kit qud cho thay mpt sd vi khuan cpng phdt d Ipn nghi suyln bao gom: Pasteurella multocida. Staphylococcus spp..
Streptococcus spp., Haemophilus parasuis. Escherichia coli vd Klebsiella pneumoniae. Kilm tra khd ndng man cdm vdi khdng sinh ciia vi khuan phan lap duac cho thay hai khdng sinh Ofloxacin va Cefotaxime cd ph6 khdng khu^ rpng, tdc ddng tdi nhieu loai vi khuan cpng phdt khac nhau. Hai sdn phdm ciia hang Pfizer la Dra.\xin va Excede cd hifu qud cao trong dilu trj bfnh suyen Ipn. Ty If khdi benh dao dpng tir 91,63 91,84%. Quy trinh phdng bfnh suyln cd thi gom vifc sOr dtmg vacxin phdng benh hodc khdng cdn dp dyng vacxin phdng bfnh. Tuy nhien, cd hai quy trinh deu phdi ddp img nhung nguyen tdc chdn nudi ndi chung nhu h?n chl sy tham quan, siir dyng chl dp cimg vdo, ciing ra,...
Tit khoa: Lpn, Bfnh suyln, Vi khudn cpng phat, Phdng trj.
Isolated additional bacteria from pigs with enzootic pneumonia and applying measure to control disease
Le Van Lanh, Huynh Thi My Le, Trinh Dinh Thau, Dang Huu Anh, Do Ngoc Thuy and Nguyen Ba Hien
SUMMARY
The additional bacteria that were found in pigs suspected mycoplasmosis include Pasteurella multocida, Staphylococcus spp., Streptococcus spp., Haemophilus parasuis, Escherichia coli and Klebsiella pneumoniae. Results of testing sensitivity to antibiotics of some bacterial Isolates show/ed that two antibiotics Ofloxacin and Cefotaxime have broad antibacterial spectrum, affecting many different bacterial communities developed. Two of Pfizer products Draxxin and Excede are highly effective in the treatment of mycoplasmosis in pigs. Cure rate ranges from 91.63% to 91.84%. Mycoplasmosis prevention process may include the use of vaccine or without vaccine application. However, both processes must meet the general principles of animal husbandry as limiting the visit, using the all in - all out. etc..
Key words: Pig, Enzootic pneumonia. Additional bacteria. Control disease.
' Khoa thu y, Trudng d?ii hpc N6ng nghifp Ha NOi.
' Vifn thii y qu6c gia
KHOA HQC KY THUAT THU Y TAP XIX S6 2-2012
LDATVAND^
Bfnh suyen Ipn hay "Djch viem ph6i dja phuang d Ipn" (Enzootic pneumonia) Id bfnh truyin nhilm man tinh d Ipn. Ty If chit do bfnh khdng cao nhung bfnh gdy ra thift hai Idn din ngdnh chdn nudi Ipn do Idm gidm t6c dO tdng trpng vd gia tdng ty If mdc cdc bfnh kl phdt, ddc bift Id nhOrng bfnh vl dudng hd h^p.
Mycoplasma hyopneumoniae (MH) Id mam bfnh chinh gdy djch viem ph6i dja phuang d Ipn vd dupc quan tdm din nhu Id mdt trong nhOrng nguyen nhdn chu ylu gdy nen bfnh hd hap phirc hpp d Ipn (PRDC: Porcine respiratory disease complex) (Thacker, 2006).
NhOrng nghien ciiru vl vai trd ciia cdc vi khu4n kl phdt trong bfnh suyen Ipn dd dupc tiln hdnh (Nguyen Ngpc Nhien, 1996; Cii HQ-u Phu, 2005; Trdn Huy Todn, 2009). Vifc dilu trj bfnh viem phoi dja phuang d Ipn bdng khdng sinh cung da dupc dl cap din trong cdc bdo cao vf phuang phdp phdng trj bfnh suyen Ipn d Vift Nam vd tren thi gidi nhu vifc sir dyng Tetracycline dieu trj Mycoplasma hyopneumoniae (Inamoto vd cs, 1994); nghien ciru sir dyng cdc khang sinh nhdm macrolide, lincosamide vd fluoroquinole trong dieu trj bfnh (Vicca, 2004); thu nghifm mpt sd phdc do dieu trj bfnh viem phoi do Mycoplasma hyopneumoniea gdy ra d Ipn (Ddm Vdn Phdi, 2006). Tuy vay cho din nay van chua thi tao ra dupc khdng sinh ddc trj doi vdi bfnh suyen Ipn.
Maes (2008) dd tong hpp nhilu nghien ciiu cua cdc nhd khoa hpc ve bfnh suyen lpn va neu y kiln vl nhQ'ng gidi phdp tong hpp dl kilm sodt bfnh. Trong dd vifc \\x ly nhChig mam bfnh kl phdt ddng vai trd quan trpng trpng cdng tdc phdng chong bfnh. Chung tdi tiln hdnh nghifn ciru nay nhdm myc dich phdn l|p vi khuin kl phat d Ipn nghi mdc bfnh suyen, kilm tra khd ndng min cdm khdng sinh dl xdy dyng bifn phap phdng trj bfnh.
II. Vi^T U$U VA PIIirOfNG PHAP NGHlfiN CtJU 2.1. V$t lifu
Chung vi khuln phan ISp dupc tir bfnh ph4m Ipm nghi mdc suyen tai 4 tinh/thdnh ph6 Ha Ndi. Bdc Ninh, Hung Yf n vd Hdi Duomg.
- Mdi trudng th^ch Mueller Hinton fOxoid).
- Cdc lo^i giiy tim khdng sinh (Oxoid).
- Cdc lo^i thu6c dilu tri thir nghifm.
2.2. Phutmg phdp nghifn ciru
2.2.1. Phuffng phdp phdn ldp vi khudn
Liy bfnh phim ph6i cd diu hifu bfnh tich viem ph6i ciia nhOng Ipn nghi bfnh 6m hodc chet dupc m6 khdm. Liy tir 1/3 khi qudn phia dudi din hit Id phoi. Bdo qudn giiri ve phdng thf nghifm trong vdng 24 gid vd phan ldp vi khuan theo quy trinh ciia Vifn thii y qu6c gia va Bp mdn vi sinh vdt - truyin nhiem. Khoa thii y, Trudng d^i hpc Ndng nghif p Ha Npi.
2.2.2. Phuffng phdp kiim tra khd ndng mdn cdm v&i khdng sinh ciia vi khudn
Khd ndng man cdm vdi khdng sinh cua cdc chung vi khuin phan ldp dupc kilm tra bdng phuorng phdp khuech tdn tren dTa th?ich vd danh gid kit qud theo Hpi dong quoc gia Hoa ky vf cac tieu chuin lam sdng phdng thi nghifm (National Committee of Clinical Laboratory Standards NCCLS, 1999)
Cdch tiln hdnh nhu sau:
+ Budc 1: Chuin bj mdi trudng thach dta Muller Hinton
+ Budc 2: Cdc chiing vi khuin dupc nudi ciy trong mdi trudng BHI (Brain Heart Infusion) 37°C/24h. Pha canh trimg nudi ciy vdi nudc sinh Iy dl d^t dp dye 0,5 trong ddy so mdu McFarland.
Dung tdm bdng vd triing, tam dung djch da pha lodng vd ddn diu len thach dia Muller Hinton.
+ Budc 3: Ddt cdc khoanh giiy tim khang sinh cua hdng Oxoid (Anh) len mdt th^ch.
KHOA HQC KY THUAT THU Y TAP XIX SO 2 - 2012
+ Budc 4: Ddt dTa th^ch d 37°C trong 18 24 gid. Dpc kit qua bdng each diing thudc k?p panme do dudng kinh vdng vd khuan va so sanli vdi bang chuin dl ddnh gia mure dp man cdm khang sinh ctia cdc chung vi khuin kilm tra.
2.2.3. Phuffng phdp thu nghifm phdc do phong tri bfnh suyin l^n
Bifn phap phdng trj bfnh suyen dupc xay dimg cdn cir vao kit qud kilm tra khdng sinli do.
Bien phdp phdng trj dupc siJr dyng thur nghifm tren nhumg Ipn nghi mdc bfnh tai cdc trai. Bifn phdp phdng trj d6i chimg Id bifn phdp dd timg dupc su dyng tai cdc tr^i vd da cd kit qud dilu trj theo dieu tra djch te hpc hdi curu. Ket qud phdng trj dupc ghi chfp vd phan tich so lifu theo phuang phdp thdng ke sinh hpc.
2.2.4. Phuffng phdp xir ly so liiu
Phuong phdp thong ke sinh hpc, sur dyng phan mem Excel 2007, WinEpiscope 2.0 va Minitab 14.
III. Ki:T QUA NGHlfeN CtTU VA THAO LU^N 3.1. Kit qud phdn l^p mft s6 vi khuin cfng phdt vdi Mycoplasma hyopneumoniae tir phoi Igrn nghi mdc suyen
Trong cdc ndm 2010, 2011. chiing tdi kilm tra trifu chirng, bfnh tich ciia nhQ'ng Ipn bj bfnh dudng hd hip. Kit qud kilm tra thiy cd 634 lpn bilu hifn trifu chiing, bfnh tich ddc trung ciia bfnh suyen Ipn bao gom: Lpn sdt, gay, ho nhilu vao sang sdm va chilu mupn, thd thi byng va thd trong tu thi ng6i, chdy nudc mui, tich djch d khi qudn va viem ph6i ddi xirng. Chung tdi dd thu thdp cdc miu phoi Ipn bfnh vd tien hanh phan Idp cdc vi khuin tren 106 mau t^i Vifn thii y quoc gia va Bp mdn vi sinh vdt truyin nhiem, Khoa thu y, Dai hpc Ndng nghifp Ha Npi. Ket qua dupc thi hifn tai bdng 1.
Bang 1. Kdt qua phan l|p mpt sd vi khuin cpng phdt vdi Mycoplasma hyopneumoniae tir phdi iQ'n nghi mdc bfnh suyln
STT 1 2 3 4 5 6
Vi khuan Staphylococcus spp.
Streptococcus spp.
Pasteurella multocida Escherichia coli Klebsiella pneumoniae Haemophilus parasuis
S6 miu xet nghifm
106 106 106 106 106 106
S6 mau duang tinh
54 45 58 27 25 12
Ty If (%) 50.94 42.45 54.72 25,47 23,58 11.32
1
95% Cl 41.15-60.74 32,77-52.14 44.96 - 64.47 16.93-34,01 15,26-31,91 5.11 -17,53
Qua bang 1 cho thiy, khi phan lap 106 mau 6k tim vi khuin cdng phat vdi MH thi bit gdp sy cd mat ciia vi khuin Pasteurella multocida cao nhit vdi 58 miu ducmg tfnh, chilm ty If 54,72%, tilp din Id Staphylococcus spp. va Streptococcus spp. lin lupt cd sd miu duomg tinh la 54 vd 45
miu, chilm ty If 50,94% va 42.45%, Escherichia coli cd 27 mau duomg tfnh, (25.47%), Klebsiella pneumoniae vdi 25 mau duomg tfnh (23.58%); it
nhat Id Haemophilus pamsuis vdi 12 mdu duomg tfnh (11,32%). Kit qud tren cho thiy, khi lpn mdc bfnh suyen thi sy lay nhilm cdc vi khuin khac la
KHOA HQC KY THUAT THU Y TAP XIX S6 2 - 2012
rat Idn vd nhOmg Io?ii vi khuin ndy cd thf Id nhOrng nguyen nhan tiem in gdy bfnh vf dudng hd hip cho Ipn. Khi chdn nudi lpn tfp trung vdi quy md ldn, mft dp ddn qud cap, dilu kifn chuong tr?ii, vf sinh, chdm sdc nudi dudng kf m laim cho sire dl khdng ciia Ipm bj gidm siit Id nhu"ng dilu kifn thudn lpi cho nhO-ng Io?ii vi khuin ndy cd dilu kifn phdt triln, tdng cudng dpc lyc vd gay bfnh cho lpn.
NhQ'ng chiing vi khuin phan ldp deu dupc kilm tra vd mang diy dii nhOmg ddc tfnh sinh vdt hda hpc ddc trung ciia chiing.
3.2. Ket qud kilm tra svr min cdm vdi khdng sinh cua mOt s6 vi khuin phdn l|p
D I xdc djnh sy min cdm khdng sinh ciia mpt s6 lo^i vi khuin phdn ldp, chiing tdi tiln hanh thir khdng sinh db. Kit qud dupc thi hifn tai bdng 2.
Bdng 2. K^t qud kiim tra
Loai khdng sinh
j
1
Amoxicillin Cefotaxime Cephalexin Ciprofloxacin Collstin Doxycyclin Erythromycin Gentamycin Kanamycin Neomycin Novobiocin Ofloxacin Penicillin Rifampicin Streptomycin Tetracycline Trimethoprim Sulfamethoxa- zol
Vancomycin
Staphylococcus spp.
(n=
S6
Chung mdn cim 34 46 28 40 23 0 38
8 0 46 50 47 41 51 0 12
45
52 54)
Tyl$
(%)
62.96 85.19 51.85 74.07 42.59 0.00 70.37 14.81 0.00 85,19 92.59 87.04 75.93 94.44 0.00 22,22
83.33
96.30
Bl/ min cdm vdi khdng sinh ciia mdt s6 vi khuin phdn ldp
Streptococcus spp.
(n=45)
S6
chung mdn cdm 35 42 28 19 5 0 40 20 0 38 31 30 36 35 0 10
24
26
Tyl$
(%)
77.78 93.33 62.22 42.22 11.11 0.00 88.89 44.44 0,00 84.44 68.89 66.67 80.00 77.78 0.00 22.22
53.33
57.78
Pasteurella multocida
(n=58)
S6
chung min cdm 52 48 49 40 32 42 39 36 41 0 2 50
0 0 54 46
35
3
Tyl$
{%)
89,66 82,76 84.48 68.97 55.17 72.41 67.24 62.07 70.69 0.00 3.45 86.21
0.00 0.00 93.10 79.31
60.34
5,17
Escherichia coll (n=27)
S6
chung min cim
23 20 20 19 21 25 18 23 20 0 0 22
0 0 18 21
19
0
Tyl$
(%)
85,19 74.07 74.07 70,37 77.78 92.59 66.67 85.19 74.07 0,00 0,00 81.48
0.00 0.00 66.67 77.78
70,37
0,00
Klebsiella pneumoniae
(n=25)
S6
chung min cdm 17 22 24 23 19 18 14 15 19 0 0 22
0 0 18 14
13
0
Tyi«
{%)
68.00 88.00 96.00 92.00 76.00 72.00 56.00 60.00 76.00 0.00 0.00 88.00
0.00 0.00 72.00 56,00
52.00
0,00
Haemophilus parasuis
(n=12)
S6
chung min cim
1
Tyl$(%)
10 1 83.33 10 1 83.33 11
12 9 11 10 6 9 0 0 11
0 1 6 5
2
1
91.67 100.00
75.00 91.67 83.33 I 50.00 75.00 0,00 0,00 91.67
0.00 8,33 50.00 41,67
16,67
8.33
KHOA HQC KY THUAT THU Y T A P XIX S6 2 - 2012
Qua bdng 2 cho thay:
Vi khuan Staphylococcus spp. min cdm cao nhit vdi khang sinh Vancomycin, chilim ty If 96,3%, tilp din Id Rifampicin (94,44%), Novobiocin, (92,59%). Gentamycin (14,81%).
Rieng Staphylococcus spp. khdng min cdm vdi ba loai khdng sinh Id Doxycyclin, Kanamycin va Streptomycin.
Vi khudn Streptococcus spp. man cdm cao nhat vdi khdng sinh Cefotaxime, chilm ty If 93,33%, tilp din la Erythromycin (88,89%), Neomycin (84,44%). Colistin (14,81%). Rieng Streptococcus spp. khdng mdn cdm vdi ba lo^i khdng sinh Id Doxycyclin, Kanamycin vd Streptomycin.
Vi khuan Pasteurella multocida min cdm cao nhdt vdi khang sinh Streptomycin, chiem ty If 93,10%. tilp din Id Amoxicillin (89,66%), Ofloxacin (86,21%), Novobiocin (3.45%). Rieng Pasteurella multocida khdng min cdm vdi ba loai khdng sinh Id Neomycin, Penicillin vd Rifampicin.
Vi khudn Escherichia coli min cdm cao nhdt vdi khdng sinh Doxycyclin, chilm ty If 92,59%, tilp din Id Amoxicillin va Gentamycin cimg chiem ty If 85,19%, Ofloxacin (81,48%), Eiythromycin va Streptomycin cimg chilm ty If 66,67%. Rieng Escherichia coli khdng mdn cdm vdi ndm loai khdng sinh la Neomycin, Novobiocin, Penicillin, Rifampicin va Vancomycin.
Vi khuin Klebsiella pneumoniae min cam cao nhit vdi khdng sinh Cephalexin, chilm ty If 96%, tilp din Id Ciprofloxacin (92%), Cefotaxime vd Ofloxacin cimg chilm ty If 88%.
Sulfamethoxazole (52%). Rieng Klebsiella pneumoniae khdng min cam vdi ndm \o^\ khang
sinh la Neomycin, Novobiocin, Penicillin, Rifampicin va Vancomycin.
Vi khuin Haemophilus parasuis min cam cao nhit vdi khang sinh Ciprofloxacin, chilm ty If
100%, tilp din la Cephalexin, Doxycyclin va Ofloxacin cimg chilm ty If 91,67%, Amoxicillin,
Cefotaxime vd Erythromycin ciing chilm ty If 83,33%. Rifampicin vd Vancomycin ciing chilm ty If 8,33%. Rifng Haemophilus parasuis khdng man cdm vdi ba Io?ii khdng sinh Id Neomycin, Novobiocin vd Penicillin.
Kit qud kilm tra sy min cdm vdi khdng sinh ciia mOt s6 vi khuin phan lap dupc cua chiing tdi cung phit hpp vdi kit qud nghien curu ctia tdc gid Cii HOru Phii vd cs (2005). Kit qua nay cung la co sd dl lya chpn khdng sinh dilu trj bfnh do cdc vi khuin tren gdy ra cho Ipn.
3.3. Xdy dyng vd thur nghifm phdc dS dieu trj bfnh suyen lpn
Tir kit qud kilm tra sy min cdm vdi khdng sinh ciia mpt so vi khuin cpng phdt d Ipn bj bfnh suyen, chiing tdi nhdn thiy hai khdng sinh cd hifu qud tdng hpp tren nhilu loai vi khuin nhat la Ofloxacin (thupc nhdm Quinolon) va Cefotaxime (thupc nhdm Cefalosporin thi hf III). Tuy nhien, cung nhu nhumg Quinolon khac. Ofloxacin cd the kfch thfch than kinh trung uang gay ra trifu churng run riy, bdn chdn. Ngodi ra nhumg cdng trinh nghien curu d dpng vdt cho thay cd nguy ca gay ra bfnh d khdp, ddc bift Id nhumg khdp chju dyng sure ndng. Mdt khac, benh suyen Igm Id bfnh d thi man tfnh vd qua trinh dieu trj can keo dai.
Do dd chiing tdi khdng lya chpn sii dyng Ofloxacin vdo phdc dd dilu trj bfnh suyen Ipn.
Qua tim hilu thdng tin vl thuoc thii y dupc phep luu hdnh t^i Vift Nam, chiing tdi lya chpn hai sdn phim dl dilu trj bfnh suyen Igm cua hang Pfizer la Draxxin vd Excede.
Thanh phin chfnh ciia Draxxin Id Tulathromycin. Day Id khdng sinh mdi thupc nhdm Triamilide, mpt nhdnh ciia nhdm macrolide chuyen ddc trj cdc bfnh dudng hd hip. Cdc nha khoa hpc dd nghifn cuu vd thiy rdng Tulathromycin cd tdc dyng dilu trj vdi Actinobacillus pleuropneumoniae, Pasteurella multocida, Bordetella bronchiseptica. Haemophilus parasuis vk Mycoplasma hyopneumoniae.
KHOA HQC KY THUAT THU Y TAP XIX S 6 2 - 2012
Thanh phan chfnh cua Excede Id Ceftiofur.
Cung nhu Cefotaxime, ddy Id khdng sinh thuOc nhdm Cephalosporin thi hf III. cd ph6 khdng khudn md rpng tren nhumg vi khuin Gram dm vd Gram duomg, bao g6m cd cdc vi khuin sinh P- lactamase. Trong qud trinh dilu tra vd thyc hifn de tai vdi npi dung dieu tri bfnh suyen Ipn, chiing tdi liln hanh thu thdp phdc do dd timg dupc dilu tn tai cac trai nhdm myc dich nghifn curu. so sdnh hifu
qud dilu trj vdi phdc d6 mdi md chiing tdi dy kiln khuyin cdo dua vdo difu trj bfnh suyln Ipm t^i cdc tinh. thdnh ph6 tham gia dl tdi. Kit qud dilu tra h6i ciai cho thiy. dl dieu trj bfnh suyen Ipn. cdc nhd chdn nudi thudng vln sir dyng cdc thuoc c6 thdnh phin chfnh Id Tiamulin va Tylosin. Chung tdi dd tim hilu. thir nghifm va so sanh hifu qud dilu trj bfnh suyen Ipn ciia cdc phdc d6. Kit qud dupc thi hifn t?ii bdng 3.
Bdng 3. Kit qud diiu trj bfnh suyin Ig^n
Phdc dd l a l b 2a 2b
Thdnh phin Tiamulin 10%
Tylosin 10%
Draxxin Excecje
Sd con dupc dif u trj
215 307 196 227
SA con khdi
147 194 180 208
S6 con khdng khdi 68
113 16 19
Ty If khdi (%) 68.37 63.19 91.84 91,63 Ghi chii: - Phdc do I: Phac do dd tirng duprc su dung lai cdc trai (Phdc do cii)
- Phdc do 2: Phdc do di nghf
Qua bang 3 cho thdy: Dilu trj bdng Tiamulin cd 147/215 con khdi bfnh, dat ty If 68,37%,
•Tylosin cd 194/307 con khdi bfnh. dat 63.19%.
Phdc dd mdi md chiing tdi de nghj dupc dua vdo dieu tri Id khang sinh Draxxin vdi kit qud dilu trj 180/196 con khdi bfnh, dat ty If 91,84% va Excede vdi 208/227 con khdi bfnh, d^t ty If 91,63%. Qua tinh todn cdc chl so OR = 5,884 va RR = 1,404, chiing tdi kit lu^n phdc do de nghj cd khd ndng dilu trj khdi bfnh cao hem phdc do cu.
3.4. Xay dyng vd thiir nghifm quy trinh phdng bfnh suyln Igrn
Trong qua trinh dilu tra. thyc hifn de tai, chiing tdi dd thu thdp dupc quy trinh phdng bfnh dd timg dupc dp dyng t^ii cdc trang tr^ii chdn nudi Ipn cd nhOmg quy trinh phdng trj bfnh hifu qud. Tren ca sd dd, chiing tdi nghifn ciiru va dl xuit quy trinh phdng bfnh nhu sau vdi cdc sdn phim sir dyng cho quy trinh dupc cung cip bdi hang Pfizer.
Quy trinh chung sir dung cho cdc trgi:
Ap dyng quy trinh ciing vdo - ciing ra (all in - all out).
- Han chl sy theun quan.
Tudn thii nghiem ngdt quy djnh sdt triing trudc khi vao trai.
Quy trinh I: Sir d^ng quy trinh chung vd vacxin phong bfnh suyin lpn
Quy trinh ndy dupc sir dyng kfm vdi ljch tiem phdng vacxin vd sir dyng thudc de phdng cac bfnh khde d lpn. Rieng doi vdi bfnh suyen, ljch trinh sir dyng vacxin gom:
3 ngay: Tifm vacxin Respisure 1 one mifn djch k^o ddi den 26 tuan.
6 thdng: Tifm vacxin Respisure 1 one cho lpn h$u bj.
2 tuin trudc khi sinh: Tiem vacxin Respisure 1 one cho 1pm ndi nhdm myc dich gidm bdi xuat M.hyopneuminae ra mdi trudng
KHOA HQC KY THUAT THU Y TAP XIX S 6 2 - 2012
Quy trinh 2: Su- difng quy trinh chung vd khong su dyng vacxin phdng b^nh suyin l(jtn Lira tudi
1 ngay 7 ngdy
21-24 ngay
28 ngay
7 tuan
Thudc phdng Mycocine 0,1 ml/con
Mycocine 0,2ml/con
Tidm b i p NAOCLIN LA: 1 ml/con
Trdn COLIMED (ENRADIN, VALOSIN) vdo thirc dn lifu: 0.5kg/tin thuc dn. Cho dn lidn tue 7 ngdy.
Trdn Tiamulin 1 kg/tin thu-c dn cho dung lidn tyc 10 ngdy
\f1axcare Vitasoi 1 kg/tin thirc dn, trfn cho dn ho$c udng lidn tyc 5 ngdy. Ldp lai sau 4 tuin.
Tidm Lincoject 1 ml/10kg t h i trong phdng Mycoplasma
Sau 7 tuin tudi thi cu 1 thdng trdn khdng sinh phdng 7 ngdy (Khdng sinh cd thf thay ddi glQ-a cac thdng) nhu sau:
Infamix (1 kg/tin thirc dn), Nao-spectin premix, Tiam premix, Valosin, Enradin....
Hai quy trinh phdng bfnh suyen Ipn deu dd dupc kiem chirng tai mpt so trai chdn nudi Ipn vd dat hifu qud phdng bfnh cao.
IV.KfiTLUAN
- 0 lpn bfnh, trong dudng hd hip thudng gdp cac vi khudn cpng phdt nhu: Pasteurella multocida (54,72%), Staphylococcus spp.
(50,94%), Streptococcus spp. (42,45%), Haemophilus parasuis (11,32%). Ddc bift cdn tim thay mdt sd loai thudc hp vi khuin dudng rudt nhu Escherichia coli (25,47%) va Klebsiella pneumoniae (23,58%). Ket qud kilm tra sy man cam vdi khdng sinh cua mpt sd vi khuin phdn lap dupc cho thay 2 khdng sinh Ofloxacin va Cefotaxime cd phd khdng khuin rpng, tac dpng tdi nhieu lo^i vi khuin cpng phdt khde nhau.
- Hai sdn phim cua hang Pfizer la Draxxin vd Excede cd hifu qud cao trong dilu trj bfnh suyen lpn. Ty If khdi bfnh dao dpng tir 91,63 - 91,84%.
Ap dyng hai quy trinh phdng bfnh suyen (1 va 2) da xac djnh cd hifu qua rd ret trong vifc khdng che bfnh.
TAI U $ U THAM KHAO
1. Nguyin Ngoc Nhien, 1996. Vai tro ciia mpt so vi khuin dudng hd hip trong hfi chimg ho thd truyin nhilm d lpn va bien phap phong tri. Ludn an tiln sT khoa hpc n6ng nghifp, trang 59.
2. Ddm van Phdi, Ph?im Lan Huomg vd Ddo Cdng Duin, 2006. Kit qud thir nghifm mft s6 phdc do dilu tri b$nh viem phii do Mycoplasma hyopneumoniea gay ra or Igm. Tap chi KHKT thu y S(4)-56-.59.
3. Cii HOu Phu, Nguyen Ngpc Nhien, Nguyen Thu Hang, Au Xuan Tuin. Nguyin Bich Thuy. Vu Ngoc Quy (2005). Xdc djnh nguyen nhan gay bfnh dudng ho hip ciia Igm nuoi tai mgt s6 tinh phia Bac. Tap chi KHKT thu y 7 (4).
4. Trin Huy Toan (2009). Dilu tra, xac djnh benh viem phSi dja phuomg do Mycoplasma hyopneumoniae va mgt s6 vi khuan cgng phat khac gay ra cho Igm tren dja ban thanh phd Hai Phong, ^h xuit bien phap phong tri. Luan van th^c sT nong nghifp.
5. D. Maes. J. Scgales, T. Meyns, M. Sibila, M.
Pieters, F. Haesebrouck, 2008. Control of Mycoplasma hyopneumoniae infections in pigs.
Veterinary Microbiology 126. 297-309
6. Inamoto, T., Takahashi, H.. Yamamoto, K., Nakai, Y., Ogimoto, K.. 1994. Antibiotic susceptibility of Mycoplasma hyopneumoniae isolated from swine. J. Vet. Med. Sci. 56. 393-394.
7. Thacker, E., 2006. Mycoplasmal diseases. In: Straw.
B.E., Zimmerman. J.J., D'Allaire. S., Taylor. D.J.
(Eds.), Diseases of Swine. 9th ed. Blacwell Publishing Ltd.. Oxford, UK, pp. 701-717.
8. Vicca, J., Stakenborg, T, Maes, D., Butaye, P., Peeters, J., de Kruif, A.. Haesebrouck. F., 2004. In vitro susceptibilities of Mycoplasma hyopneumoniae field isolates. Antimicrob. Agents Chemother. 48. 4470-4472.
