V i e t n a m Journal of Physiology 21(1), 3 / 2 0 1 7 I S S N : 1 8 5 9 - 2 3 7 6
SUMMARY
CHANGES IN FUNCTIONS OF THE CARDIOVASCULAR SYSTEMAT 1WEEK AFTER FIXING HYDROXYAPATIT (HAp) TO DOGS' FEMURS
Can Van M a o \ Nguyen Minh Nui^
Wietnam Military Medical University In the present study, we evaluted changes in some parameters of ECG between before and after surgeries to fixed a biomaterial HAp on dogs' femurs. Results showed that there were no significant differences in amplitudes and durations of waves as well as intervals in ECG recordings. These results indicated that HAp have a few effects to functions of the cardiovascular system and HAp has high biocompatible properties. Thus, HAp might be a good biomaterial to apply for treatments in humans.
Keywords: HAp, ECG recording. Dogs.
X A Y DU'NG M d HiNH N H 6 | M A U N A O T R E N C H U O T N H A T B A N G PHU'ONG P H A P QUANG H 6 A
Dang Thanh C h u n g \ Can Van Mao^' TOM T A T : Myc tidu: Trong nghien cwu ndy chung tdi tiin hdnh xay dung md hinh nhdi mau ddng mach ndo giira trdn chudt nhdt. Doi twgng va phwong phap: md hinh nhdi mdu ddng maeh nao giu'a dwgc thyc hidn bing edeh sCe dung chit mdu cam quang rose begal dwgc dwa vao he thdng tuin hodn cua chudt, vd dOng tia krypton laser v&i bw&c sdng Id 568nm chiiu vao ddng mach nao giira qua xwang sg cOa chudt, rose bengal tai vj tri dwgc chieu sd hinh thdnh gdc ty do gay tdn thwang ti bao ngi mac mach mdu vd kich hoat hinh thdnh cue huyit khdi gay nhdi mdu. Kit qua: ChOng tdi da xac djnh dwgc khoang th&i gian tdi wu chiiu tia laser di tao dwge 6 nhdi mdu Id 4 phOt, v&i ti Id gay thdnh edng bing phwang phdp ndy Id 100%,. Thi tich khdi nhoi mdu (gia trj trung binh ± SEM; mm^) a eae th&i diem 3 ngay, 7 ngay va 14 ngdy sau nhdi mdu lan Iwgt la:
267,7 ± 42,7, 219,7 + 4,4; 205,7 ± 35,1. Kit lu$n:Chung tdi dd xay dyng thdnh cdng md hinh gay nhoi mau nao tren chugt nhit tring twang ty nhw tinh trang b$nh iy trdn ngw&i, Idm phwang tidn hiru dyng di thir nghidm cdc phwang phdp dieu trj nhoi mau mai.
Tir khoa: nhdi mau, dgng mach nao giira. krypton laser, rose bengal.
1. OAT V A N O £ nguyen n h i n gay t u vong dOng h i n g t h u Dot quy nao anh hudng den k h o i n g hai sau benh mgch v i n h d c i c n u d e p h i t 15 trieu ngudi tren t o i n c l u mdi nam va l i trien [1] d i y cung l i nguyen nhan hang
dau g i y t i n t$t vdi ti le t i n t i t vTnh vien trdn 50% nhung ngudi song sdt sau dot quy. Dot quy d u g c chia t h i n h t h i x u l t
•" Hoc vi§n Quan y h u y i t hogc tac mgch/thleu mau cue b i
• Tac gia chju trach nhiem chinh: (nhoi m i u ) , trong dd da s i c i c trudng hap Can Van Mao
Email: [email protected] Ngay nh^nbSi: 21/12/2016 Ngay nhan phan bien: 22/2/2017 Ngay chap nhan dang: 23/2/2017
(80%) rai vao t h i nhdi m i u [2].
B e nghien cOu ca c h i trong benh ly trong nhoi m i u nao va tim kiem cac phuang p h i p , c i c logi thuoc mdi dieu tn nhoi m i u nao, md hinh dpng v i t bpnh ly n h i i m i u nao l i yeu t i quan trpng khdng the t h i i u de cd t h i d i p Ong d u g c c i c yeu c l u tren. H l n g nam tren the gidl cd r l t n h i i u cac nghien cOu da d u a c x u l t b i n tren c i c tgp chi chuyen nganh m i cd s u dung cac md hinh nghien cOu nhoi mau nao tren dgng vat t h y c nghiem. [2], [3], [4].
Md hinh g i y n h i i m i u d u g c thiet lgp d mpt s i Ipai nhung p h i b i i n n h l t v i n la tren mdt so loai ggm n h l m n h u chudt nhat hogc chudt c i n g . Viec lya chpn doi t u g n g la loai gam nham do mdt s i d i e d i i m mang tinh uu viet cua doi tugng n i y nhu:
v i mgt y dOc d i i tugng nay d i d u a e c h i p nhgn han so vdi nhung l o i i dpng vat cd vu d c l p cao han, chl phi nudi d u d n g v i duy tri thap, cd giai p h l u mgch mau t u o n g t y nhu con ngudi. Do s y tuang d i n g cao v i c l u true g i l l phau va cac ca che chuyen hda trong ca t h i giua l o i i gam n h l m v i con ngudi nen viec d i n h g i i tac dyng tien l i m s i n g cua c i c phuang p h i p dieu tri ddt quy tren cac l o i i gam n h l m la r l t g i n vdi viec cd the i p dung tren n g u d i . Ddng mgch nao giOa (Middle cerebral artery - MCA) la mach m i u pho b i i n n h l t bi i n h hudng trong dot quy do tac mgch/thiiu m i u cyc bd v i d i y cung l i dpng mgch p h i b i i n n h l t d i tac dpng trong cac mo hinh ddt quy d loai gam nham. Gan d i y phuang p h i p hda hpc g i y cue huyet k h i i d u g c i p dung kha rdng rai n h u phugng p h i p quang hda bang each k i t hgp tiem vao hp thong tuan h o i n chung mdt chat nhay cam vdi i n h s i n g (nhu Rose bengal hoac erythosin B) vdi viec c h i i u anh sang b i n g b u d c sdng dac hidu [3]. S y tuang t i e giua anh sang v i e h l t nhgy cam vdl i n h s i n g trong ndi mgch cOa ddng vat thi nghiem se smh ra nhung g i c oxy hda v i gay oxy hda mang lipid cua te b i o ndi mgc v i c i c thanh p h l n cua m i u gay ra k i t tap
t i i u c i u va hinh t h i n h h u y i t k h i i [4].
Hien nay tren t h i gldi va Viet nam viec tim k i i m phuang p h i p mdi trong d i i u tri nhdi m i u v i n ludn la chu d i mang tinh thd'i s y , c i c thO nghiem t i i n lam sang la khdng t h i t h i i u vi the viec x i y d y n g md hinh nhdi m i u tren ddng vgt d i t i i n hanh c i c t h u nghiem t i i n l i m s i n g l i vd cung quan trpng v i c l p thiit.
2. £)6l TU'aNG VA P H U O N G P H A P NGHIEN CU'U
2 . 1 . Vat lieu nghien c u u - Ddng vgt
Hai mugl hai chudt nhat trang g i i n g Swiss, 10 - 12 t u i n tuoi (22 - 25 g), cung c l p bdi Vien vd sinh djch te Trung Lfang.
Chugt d u g c nudi trong dieu kien chudng thoang mat, dam bao ve sinh, c h i dp an uong theo nhu c l u cOa. Chudt d u g c chia thanh eac nhdm:
+ Nhdm xae djnh thai gian c h i i u laser (n=9): vdi ba thai 2, 4 v i 6 phut phliu tia laser
+ Nhdm x i c dinh tinh dae hieu (n=3):
tiem NaCl 0.9% va c h i i u laser + Nhdm x i c dinh tinh lap Igl (n=10) tiem Rose Bengal v i c h i i u laser d ba thdi d i i m 3 n g i y (n=3), 7 n g i y (n=3) v i 14 n g i y (n=4).
- Dung cy, hda c h i t va cac phuang tien can t h i i t cho thu thuat g i y nhdi mau nao
+ Bp dung cy vi phau (Fine Science Tools, Held elberg, Germany)
+ Bp sterotaxlc de cd djnh chudt va tay giO nguon c h i i u tia laser (Nanshige, Japan)
+ Dyng cy phat tia Krypton laser (budc sdng 568 nm, cong s u i t 6 mW).
+ Rose Bengal (Sigma-Aldrich 198250- 5G), Ketamlne (50 mg/mi, Germany)
+ Dyng cy hda c h i t d^ c i t va nhudm nao chudt: dao lam (Croma, Germany), Adult Mouse Brain Slicer, thuoc nhudm- 2, 3, 5 - triphenyl tetrazoliumchlorlde (TTC) (Slgma-Aldrich).
2.2. Phuo'ng phap n g h i e n cOu
V i e t n a m Journal of Physiology 21(1), 3 / 2 0 1 7 I S S N : 1 8 5 9 - 2 3 7 6
- Chuan bj t h u i c Rose Bengal, chon l i i u v i d u d n g dua t h u i c
Rose Bengal d u g c hda tan trong NaCI 0,9% (15 mg/ml) vdl l i i u 20 mg/ kg can ngng cua chudt, theo nghien cOu d i y tren
chudt cong [5].
Rose Bengal d u g c d u a v i o h$
thong tuan h o i n cua chupt b i n g c i c h tiem qua xoang tTnh mgch quanh hoc mat [6] (Hinh 3.1)
l>
Hinh 3.1. D u a thuoc Rose Bengal v i o he t h i n g t u i n h o i n cua chudt qua - Tgo 6 n h i i mau va tdi uu thdi gian
c h i i u laser d i tgo h u y i t k h i i g i y n h i l mau + Nguyen ly tgo i nhoi m i u b i n g phuang phap quang hda. C h i t m l u c i m quang tiem v i o hd t h i n g tuan h o i n , i n h s i n g cd buOc sdng thich hap c h i i u tO phia n g o i i v i o d i n tdi hinh t h i n h cac g i c t y do, g i y oxy hda va gay ton t i b i o b i o ndi mgc mgch m i u , kich hogt ca c h i k i t t i p t i i u c l u hinh thanh h u y i t khdi tgi vj tri c h i i u s i n g [7]. Rose bengal l i mdt c h i t m i u c i m quang d u g c sO dyng p h i b i i n hidn nay va cd dp hap thu c y c dgi khi d u g c chieu tia Krypton laser vdl b u d c sdng l i 568 nm.
+ T i i u u thai gian c h i i u tia laser Thdi gian c h i i u tia laser d u g c toi uu b l n g c i c h x i c djnh thdi gian chieu ngan nhat de x u l t hien cyc h u y i t k h i i , d u g c nhgn b i i t qua danh g i i dai t h i luu thdng cua ddng mach bang kinh h i i n vl quang hpc v i thdng qua md benh hpc. ChCing tdi t i i n h i n h c h i i u laser vdi thdi gian d y k i i n l i 2, 4 v i 6 phijt sau dd d i chpn thdi gian ngan n h l t m i d dd hinh thanh huyet k h i i .
- Cac b u d c t i i n hanh gay nhdi m i u + Chudt d u a c g i y md b l n g Ketamine (10 mg/100 gram can nang), tiem m i n g byng, p h l n Idng d p h l n trudc va ben t r i i
d l u d u g c cao bd, s i t khuan con 70%.
+ Rgch da tO diem ngang h i e mat len dinh dau 1.5 cm va 1 cm n i i t i i p dudng t h u nhat t o dinh d l u x i n g m i trai d i bpe Id e g t h i l d u a n g trai. Tach b d tren v i sau cua ca va keo xudng d u d i , ddng mach nao giOa trai (middle cerebral artery - MCA) lp rd d u d i x u o n g so.
+ c i djnh chupt len stereotaxic va c h i i u tia Krypton laser vao chd p h i n nhanh (Hinh 2d) cua ddng mgch nao giua, quan sat s y mat luu thdng ddng mgch d u d i kinh hien vi.
- X i e djnh tinh d i e hieu v i tinh i n djnh cua phugng phap tgo md hinh
De x i c d|nh tinh d i e hidu cua phuang p h i p gay nhoi m i u l i do Rose bengal, chung tdi thO tren mdt nhdm chudt (n = 3) d u a c tidm NaCl 0.9% va c h i i u Krypton laser theo thai gian da t i i u u , t i i n h i n h quan sat luu thdng cua dpng mgch nao giua d u d i kinh h i i n vl v i d i n h ton thuang b l n g nhudm TTC. X i c djnh tinh on djnh cda phugng phap bang each tinh ti le xuat hien i n h i l miu/tong s i chupt d u g c t i i n hanh thO nghiem.
- Phugng phap tinh the tich ton t h u a n g . Chudt d u g c gay me sau (Ketamine 20 mg/100 gram c i n n^ng), sau dd bpe Id nao
V i e t n a m Journal of Physiology 21 (1), 3 / 2 0 1 7
t u a i v i dua len k h u i n c i t nao, khoang c i c h giua hai l i t cat lldn t i i p l i 1mm qua vung t h u a n g t i n .
C i c l i t c i t cua n i o sau khi nhudm TTC, d u g c chup anh d u d i kinh h i i n vi. Dien tich thuang ton (mm2) d u g c tfnh theo phuang p h i p d u g c md ta d nghidn cOu t r u d c d i y [8] bang s u dyng p h l n m i m ImageJ. The tich 6 t h u a n g ton (mm3) d u g c tinh theo cdng thOc:
V = d'[1/2 (Y1+Yn) + Y2+...Yi ..+Yn-1]
Trong dd: V l i the tich thuang t i n ; d l i k h o i n g c i c h giua hai l i t c i t lien tiep (1mm); Yl la di$n tich thuang t i n cua l i t cat thO i.
2.3. Xu> ly so lieu nghien c u u So lieu nghien cOu d u g c s u ly v i tinh t o i n s u dyng thugt t o i n t-test hogc one- way analysis of variance (ANOVA) trdn phan m i m XLSTAT 2015. K i t q u i d u a c trinh bay d u d i dgng g i i tri trung binh ± SEM. S y k h i c bidt cd y nghTa thong ke d u g c x i c djnh khi p<0,05.
3. K^T QUA
3.1. Thd'i gian chieu can t h i i t de tao cue huyet khoi
Chung tdi da t i i n hanh chieu laser d c i c khoang thdi gian 2 phut, 4 phut v i 6 phut sau khi chugt d u g c tiem Rose bengal v i o h^ t h i n g tuan hoan qua xoang tTnh mgch sau hoc mat. K i t q u i cho thay, c h i i u laser trong thdi gian 2 phut khdng l i m m i t luu thdng cua dpng mgch n i o glOa t i t ca chudt trong nhdm t h u , mac du v i y chCing tdi van tien h i n h chieu sang l l n hai ngay phia d u d i l l n chieu d l u tien trong thdi gian 2 phut (Hinh 3.2d). 6" c i c nhdm chudt c h i i u tia laser trong k h o i n g thdi gian 4 va 6 phut cho t h l y luu thdng ciJa ddng mgch nao giua bj mat sau l l n c h i i u d l u tien (Hinh 3.2a - 3.2c), d i tang cudng v i d i m b i o tfnh i n djnh vung b i n cua cyc h u y i t k h i i mdi hinh t h i n h chung tdi t i i n hanh c h i i u l l n thO hai d ngay d u d i vj tri c h i i u l l n dau (Hinh 3.2d). Nhu v i y luu lugng t u i n hoan cOa dpng mgch nao giua bl m i t d ea hai khoang thai gian 4 phut va 6 phut chieu tia laser.
Hinh 3.2. Hinh anh cua ddng mgch nao giua cua chudt trong q u i trinh tgo huyet khoi g i y 0 n h i l m i u va phuang phuang p h i p g i y k h i i nhoi m i u .
a. Hinh inh eua dpng m$ch nao giwa sau khi bpe Id- b. Hinh dnh eua ddng mach nao giira khi dang chieu Krypton laser, c. Hinh dnh dgng mach nao giwa mit Iwu thdng khi xuit hidn eye huyet khdi. d. Phwang phap gay khdi nhdi mau: sau Iin chiiu diu tien &
ngay vi tri phan chia cua ddng m^eh ndo giwa gay mit Iwu thdng, chiiu Iin hai & ngay dw&i vj tri chiiu diu tidn v&i cung khoang thai gian.
D i xac djnh c h l e chan ton t h u a n g Chung tdi t i i n h i n h nhudm TTC nao chudt 3 n g i y sau khi c h i i u tia laser. K i t q u i cho thay d nhdm chudt chieu tia laser trong
vdng 2 phut khdng thay xuat hien t i n thugng d nao bp trdn t o i n bd c i c l i t c i t (Hinh 3a). Trong khi d hai nhdm chudt c h i i u laser trong vdng 4 phut v i 6 phut ton 51
V i e t n a m Journal of Physiology 21(1), 3 / 2 0 1 7 I S S N : 1 8 5 9 - 2 3 7 6
thuang x u l t hien d tat c l c i c d i i t u g n g t h u nghiem (Hinh 3b-3c).
2 |ihul 4pliut 6 pliiil
Hinh 3.3. Hinh anh nao chudt tgi thdi d i i m 3 ngay sau khi tien h i n h gay n h i i m i u a. Ndo chudt chiiu Krypton laser 2 phut. b. Nao chupt chiiu tia Krypton laser trong ^ phiit. c. Ndo chugt chiiu tia Krypton laser trong 6 phut.
Chung tdi t i i n h i n h do t h i tich k h i i bidt cd if nghTa t h i n g ke gi&a hai n h d n n h i l m i u va so sanh tren hai nhdm c h i i u (Bleu do 3.4) Vi vgy chung tdi chpn thdi laser 4 phut v i 6 phut. K i t qua cho thay gian toi uu c h i i u tia laser d i tao i nhci the tich khdi n h i i mau khdng cd s y k h i c m i u la 4 phut
tt
Tlifn gian ciii6u tia la.'ier (phiit)T
Bleu do 3.4. The tich o n h i l mau d u g c do tgi hai khoang thai gian 4 phut v i 6 phut c h i i u tia Krypton laser.
Gid tri = Trung binh ± SEM, n = 4 cho 4 phut, va n=3 cho 6 phut.
Hinh 3.5. Nao chudt khdng bj t i n thuang khi c h i i u Krypton laser trong 4 phOt khdng cd Rose bengal d u g c thay b l n g NaCl 0.9%.
N h i m khang djnho nhoi m i u d u g c tgo hogt bd-i Krypton laser g i y ra t i n t h u g n g ra theo ca c h i do Rose bengal d u g c kich t i b i o npi mgc m i khdng p h i i do t i e ddng
tryc t i i p cua Krypton laser. Chung tdi t i i n h i n h c h i i u Krypton laser vdi thdi gian la 4 phiit tren nhdm chudt (n =3) d u g c tidm bang NaCI 0.9% thay cho Rose bengal.
K i t qua nhudm TTC cho t h l y khdng cd ton t h u a n g tren nao bp cua tat c i c i c d i i t u g n g t h u nghipm (Hinh 3.5). N h u vay ban t h i n Krypton laser khdng g i y ra 6 nhoi miunhdi m i u n i u khdng cd mat cua Rose bengal, day chinh l i tfnh dgc hieu cua phuang p h i p n i y .
3.3. Tinh lap lai cua phuo'ng phap va the tich thirang ton sau nhoi mau
Vdi myc dfch kiem chOng tinh on djnh cua phuang phap va b u d c d l u d i n h g i i s y thay doi ve the tfch cua k h i i n h i l m i u theo thd'i gian, chung tdi t i i n h i n h g i y n h i l m i u v i xac djnh ti le chudt ed 6 nhdi miu/tong so chupt t h u nghiem, d i n g thdi do t h i tfch k h i i t h u a n g ton d c i c khoang
thdi gian 3 ngay, 7 n g i y v i 14 ngay sau khi g i y dot quy.
ChOng tdi t i i n h i n h trdn 10 chudt d u g c t i i n h i n h gay nhoi m i u t h e o phuang phap da t i l uu d tren v i t i i n hanh l i y nao nhudm TCC d c i c thdi diem 3 n g i y (n=3), 7 n g i y (n=3) va 14 n g i y (n=4) sau dot quy.
K i t qua cho t h i y 6 n h i i mau x u l t hien d t i t ca chudt (Hinh 3.6 v i B i n g 3.1), nhu v i y ti le thanh cdng gay n h i i m i u b i n g phugng p h i p n i y l i 100%.
K i t qua t h i tfch khdi n h i i miu(mm3;
Gia trj trung binh ± SEM) d thdi diem 3 n g i y , 7 n g i y va 14 ngay theo thO t y nhu sau: 267,7 ± 42,7; 219,7 ± 4,4; 205,7 ± 35,1 (Hinh 3.7). N h u vgy t h i tich cua k h i i n h i l m i u ed xu hudng g l i m nhung chua cd s y k h i c biet t h i n g ke d tat ca cac thdi d i i m nghien cuu.
3d M C A O "^ ^ M C A O 14 d M C A O Hinh 3.6. Hinh anh n i o chudt d c i c thdi diem k h i c nhau sau khi gay ddt quy
4. B A N LUAN
Da cd k h i n h i i u md hinh gay n h i i m i u d a d u g c p h i t t r i i n , m i l md hinh cd nhung u u diem v i nhuge d i i m ridng biet.
Nhung md hinh d l u tien, ddng mgch nao giua d u g c bdc Id qua thu t h u i t m d hop sp,
MCA cd the d u g c lam t i c theo mgt s i phuang phap nhu: b i n g c i c h ddt dien hogc sO dung c h i t co mgch mgnh va keo dai nhu endothelln-1 [9]. Ngoai ra cd the g i y n h i l m i u khdng c l n can thiep vao hdp sp ma t i i p can thdng qua dpng mgch canh
Vietnam Journal of Physiology 21(1), 3/2017 ISSN: 1859-2376
de gay nh6l mau nhi^ day filament hoac v | t t i c mach tdi dong mach nao giu'a [10].
Bang 3.1. Ti I? thanh cong cua phu'ong ph^p gay nh6i maubing phuong phap quang C h u p l xuat h i ^ n 6 dpt quy nhoi mau l^i
cac tlicri diem (con)
Ti 16 Ihalili
3d 7d
ChiiOtlhitngli.Sn. . - ^ - 14dMCA0
(con) MCAO MCAO cong
10 100%
i
Bieu do 3.7. The tich o n h i i mau do tgi c i c thdi d i i m 3, 7 va 14 n g i y sau dot quy.
Gid tri bieu didn = Trung binh ± SEM, n = 3-4 cho mdi nhom chudt a cdc thai diem.
Nhung phuang p h i p tren cung tgo d u g c n h i i m i u nhung cung bdc Id mdt s i nhuge d i i m nhu p h i i m d hop sp ddi hdi ky t h u i t p h i i cao, phuang phap dung filament l i m t i n g nguy c a g i y chay m i u v i khdng phu hgp vdl c i c nghidn cuu n h l m lam tan cyc h u y i t k h i i , hay phuang phap dung thudc co mgch cung yeu c l u ky thuat m d hop sp v i cung khdng thich hgp cho nghidn cOu lam tan cue h u y i t k h i i , hogc khdng gan vdi ca c h i benh sinh cua nhoi m i u n h u tren thyc t i bpnh nhan.
Phuang p h i p gay n h i i m i u b l n g phuang p h i p quang hda, t i l t h i i u hda c i c yeu to can thidp, v i g i y d u a c chinh x i c cyc k h i i tai vi trf c h i i u s i n g , ti le dpng vgt c h i t sau thO thugt t h l p va vigc hinh thanh cyc huyet khoi b l n g chfnh c i c t h i n h phan npi tgi cCia mau g i n vdl c a c h i gay n h i l m i u tren benh nhan.
Mac du gay n h i i m i u bang phuang p h i p quang hda khdng cdn mdi tr§n t h i gidi va cung pho bien tren ddi tupng l i chudt c i n g , tuy nhien day cd le l i md hinh gay nhdi mau thanh cdng tren chudt n h l t trang bang phuang p h i p quang hda d l u tidn tai Vidt Nam. Vlpc xay dyng t h i n h cdng md hinh la phugng tien quan trgng giup t h u nghipm d i n h g i i mot so thuoc mdi cd tac dyng d i i u tn hogc h i t r g d i i u tn n h i l mau t r u d c khi p h i t t r i i n sO dyng dieu trj tren ngudi. Chung tdi hy vong k i t qua nghien cuu cua chung tdi se m d ra nhung hudng nghien cOu mdi trong tim ra phuang t h u i c dieu trj nhoi mau mdi hieu q u i , dgc biet l i phuang tlpn quan trpng de chf ra ca s d khoa hpc cOa mdt so thuoc ddng y hien nay dang d u g c sO dung rpng rai trong d i n gian hd trg d i i u trj n h i i m i u .
V i e t n a m Journal of Physiology 21(1). 3 / 2 0 1 7
5. K^T LUAN
Trong nghien cOu nay chung tdi da xay d y n g thanh cdng md hinh g i y nhoi m i u tren chudt n h l t t r l n g b l n g phuang phap quang hda k i t hgp giOa Krypton laser vdi budc sdng 568 nm, cdng s u i t 6 mW va Rose bengal vdi l i i u 20 mg/ kg can ngng eg t h i . Chung tdi da x i c dinh d u g c khoang thdi gian toi u u chieu tia laser d i tao d u g c 6 n h i i mau la 4 phut, vd'i ti le g i y n h i i miuthanh cdng b l n g phuang phap n i y l i 100%. Qua nghien cOu nay chung tdi b u d c d l u t h i i t lap chi so tham chieu ve the tich k h i i nhoi miu(Trung binh ± SEM;
mm3) trdn chupt nhat trang g i i n g Swiss d c i c thai diem 3 n g i y , 7 n g i y va 14 ngay sau nhoi m i u l l n lugt l i : 267,7 ± 42,7;
219,7 ± 4,4; 205,7 ± 35,1 (mm3).
T A I LIEU THAM K H A O 1. D o n n a n , G.A., et al. (2008), Stroke.
Lancet. 371(9624): p.1612-23.
2. Heiss, W.D. (2010), The concept of the penumbra: can It be translated to stroke management? Int J Stroke. 5(4):
p.290-5.
3. Cal, H., et al (1989), Photothrombotic middle cerebral ariiery occlusion in spontaneously hypertensive rats: influence of substrain, gender, and distal middle cerebral artery patterns on infarct size. Stroke,. 29(9): p 1982-6;
discussion 1986-7.
4. Ginsberg, M.D., R. B u s t o (1989),
Rodent models of cerebral ischemia.
Stroke, 1989. 20(12): p 1627-42.
S . W a t s o n , B.D., et al. (1987), Argon laser-induced arterial photothrombosis.
Characterization and possible application to therapy of arteriovenous malformations.
J Neurosurg,. 66(5): p.748-54.
e . Y a r d e n i , T., et a l . (2011), Retro- orbital injections in mice. Lab Anim (NY),.
40(5):p.155-60.
7. Kleinschnitz, C , et a l . (2008), Blocking of platelets or Intrinsic coagulation pathway-driven thrombosis does not prevent cerebral infarctions induced by photothrombosis. Stroke,. 39(4): p.1262-8.
8. Rosen, G.D., J.D. Harry (1990), Brain volume estimation from serial section measurements: a comparison of methodologies. J Neurosei Methods. 35(2):
p. 115-24.
9. Macrae, I.M. et al (1993), Endothelin-1-induced reductions in cerebral blood flow: dose dependency, time course, and neuropathological consequences. J Cereb Blood Flow Metab.
13(2): p. 276-84.
10. Z h a n g , Z., et al (1997), A mouse model of embolic focal cerebral ischemia. J Cereb Blood Flow Metab, 1997. 17(10): p.
1081-8.
SUMARY
ESTABLISH THE ISCHEMIC STROKE MODEL IN MICE BY THE PHOTOCHEMICAL METHOD
Dang Thanh C h u n g \ Can Van Mao^
*Hpc vien Quan y Objective: In this study, we conducted modeling ischemic stroke of middle cerebral artery in mice model. Subject and methods: ischemic stroke of middle cerebral artery model was established by intravenous infusion ofphotosensitive dye rose begal into the circulatory system of the mouse, and krypton laser beam wavelength is 568 nm shines on the middle cerebral artery through the mouse's skull induce rose bengal to form free
55
V i e t n a m Journal of Physiology 21(1), 3 / 2 0 1 7 I S S N : 1859 - 2 3 7 6
radicals that cause damage to vascular endothelial cells and trigger clot formation causing infarction.Results: We have identified the optimal time to create laser projection drive ischemic stroke is 4 minutes, with succeeded is 100%. Stroke volume (average
±SEM; mm^) at 3 days, 7 days and 14 days after stroke respectively are: 267,7 ± 42,7, 219,7 ± 4,4; 205.7 ± 35,1. Conclusion: We have successfully built a model of ischemic stroke in mice similar condition in humans, as a means useful for testing new method of treatment stroke.
Key words: ischemic stroke, middle cerebral artery, krypton laser, rose bengal.
T A C DONG CCiA MOI TRU-^^NG PHONG PHCI D ^ N H A N H VI LI^N QUAN K H A M P H A VA HOC TAP T R ^ N CHUOT C 6 N G D U - O C G A Y M 6 H I N H BENH TLT K t
Tran Thj Quynh Trang^"^, Dao Thu Hong^'^
Nguyin Le Chien^, Tran Hai Anh^'' T 6 M T A T : Dd tdi nghidn ciru dwgc tidn hanh v&i Myc tidu: "Ddnh gia tdc ddng cua mdi trwang phong phu (MTPP) din hanh vi Iidn quan boat ddng kham phd va hgc tap cua chudt edng da dwge gay md hinh benh ty ky bdng axit valproic". Doi twong va phwtyng phap nghien ciru: Thiit ki nghidn cieu- Theo ddi doe. md ta cit ngang. Gay md hinh bdnh ty ky trdn chudt cdng trdng bdng tidm phuc m^c axit valproic (VPA) liiu 500 mg/kg thi trong vdo ngdy 12,5 eua thai ky. Chugt con sinh ra dwgc ehia nhdm ngau nhidn, gdm cac nhdm dwge nudi 28 ngdy (tie 23 din 51 ngdy tudi) trong MTPP (chirng-PP, VPA-PP) va dwgc nudi trong mdi trw&ng chuin (ehirng-C, VPA-C) trong cung thai gian. Hanh vi cua cdc nhdm ddng v$t dwgc ddnh gia qua eae bdi tap trong mdi trw&ng ma, twang tdc xd hdi 3 budng vd trong me Id nw&c. Kit qua eho thiy: (i) MTPP Idm tang ew&ng hanh vi khdm pha trong mdi trw&ng ma; (II) cd phin lam tang hoat ddng twang tac xa hdi cung nhw (HI) tang tri nh& khdng gian trdn chugt cdng trw&c trwong thdnh. Kit ludn: MTPP cd tde dyng eai thidn hdnh vi lien quan hoat ddng khdm pha va hgc tap tren chudt cdng da dwgc gay mo hinh benh ty ky.
Tw khoa: axit valproic, ty ky, moi trw&ng phong phii, hanh vi, chugt cdng 1. DAT V A N D i tinh han hpp [1]. Hipn v l n chua cd thuoc
T y ky l i chOng benh thudc nhdm cac g^^ tn t y ky, ma d i i u trj chu y i u sO dyng rdi loan phat trien tam than lan tda, keo d i i mot so logi d u g c pham n h l m cai thien trong cugc s i n g , v i dgc trung bdi s y suy trieu chOng v i ap dyng mdt so bien p h i p giam kha nang hda n h i p xa hdi cCing kha trj Wdu t i m ly giao due. Han n u a , c i c n i n g giao t i i p , cd nhung hanh vi rap pghien cOu d trong nude hien t h u d n g khudn, lap Igi va nhung quan t i m mang quan t i m v i khia cgnh djch t i hpc va dac
diem l i m sang cua benh, cdn nhung
^ Hpc vi§n Quan y nghien cOu thyc nghiem v i nghien c u u
^ Dgi hpc Y Dupc H^i Phong can thiep tren md hinh ddng v i t bi g i y 3 ^ ™ A n h " * ' * " " " " * b S n h t v k y v a n c o n h a n c h l Email: anhhlriaivahoo-com Nhleu nghidn cOu da sO dung axit S I t SivS'n'S"27,3,20,7 valproic d J gay mo hInh b«nh tu- ky t r i n Ngay chSp nhSn dSng, 28/3/2017
