Tnrang Dai hpc Vinh Tgp chi khoa hpc. Tdp 45. So 3A (2016), tr. 77-87
PHAN LAP VA TUYEN CHON MQT SO CHUNG TAG LUC GI4U LIPID 6 HO CHlTA NUdC THAI DO THI VINH TAN
(THANH PHO VINH, NGHE AN) Nguyin Le Ai Vinh, Pham Thi Nhu Quynh
L§ Thi Vka Anh, Triromg Thj Hoai An Khoa Sinh hoc. Tnrang Dgi hgc Vmh Ngay nhan bai 26/9/2016, ngay nhan dang 17/11/2016
Tom tat: Ho Vinh Tan (thanh pho Vinh, Nghe An) la mpt frong nhiing noi tilp nhan ngudn nude thai cua thanh phd Vinh. Ket qua dieu tra cho thay nude hd da bi d nhilm hihi CO v^ 6 nhilm amoni. Doi vdi nganh tao luc (Chloropbyta), co 14 loai va dudi loai dugc tim thiy phin b6 trong h6 Vinh Tan. Tam chung tao luc thuoc cac loai Scenedesmus obliquus (3 chung), S. quadricauda (3 chung) \k Chlorella pyrenoidosa (2 chung) da dugc phan lap thanh cong, trong do, chung S. quadricauda VT-3C va S. quadricauda VT-5C c6 kha nang tich luy lipid tot nhat. Trong m6i tmdng AF-6, thdi gian nuoi ngan nhat de dat dugc mat do tl bao va ham lugng lipid cao nhit la 14 ngay; mat do tl bao cua chung VT-3C va VT-5C frrong ling la 138,39x10* vi 138,42x10* tl bao/mL; ty le lipid ciia chiing VT-3C va VT-5C dat tuong irng la 28,4% va 26,3% sinh khdi khd.
I. DAT VAN DE
Ngay nay, vi tao dugc xem la mdt frong nhiing ddi tuong tiem nang ifrig dung de san xuat diesel sinh hgc [5, 9] bdi vi cbiing cd kba nang tich liiy triacylglycerol vdi mdt ham lugng cao trong tt bao [12]. Mat khac, mdt sd loai nude thai co the su dung de nudi vi tao lay sinh khdi [10, 11]. Do dd, sy ket hgp ca hai each tiep can nay se la mpt giai phap tdt vua de xir ly nude thai, vira de gdp phan giai quyet van de tbieu hut nang lupng.
Giai phap nay da dugc xay dung thanh chien luge dai ban cap qudc gia d mpt sd nude nhu Hoa Ky, Nhat Ban, Han Qudc, Ausfralia... ma diem khdi dau la phan lap, tuyen chpn dugc nhung chiing vi tao giau lipid, thich nghi tdt vdi dieu kien kbi bau dia phuang va vdi loai mdi tmong nude se su dung vao san xuat sinh khdi [9].
Tao luc (Chloropbyta) da dang ve thanh phan loai va pban bd rdng rai frong cac loai mdi tradng nude khac nbau [6]. Mdt sd loai giau lipid tbudc cac cbi Botryococcus, Chlamydomonas, Chlorella, Dunaliella, Scenedesmus... da dugc ngbien ciiu nudi frong cac loai nude thai khac nhau va cho nang suat sinh khdi, ham lugng lipid khac nhau [7, 11]. Trong nghien ciiu nay, cbiing tdi tap trang pban lap, danh gia va tuyen chgn mdt sd chung tao luc giau lipid tir hd cbiia nude thai dd thi Vinh Tan (thanh phd Vinh, Nghe An) de tao nguyen lieu cho cac nghien ciiu sau han theo hudng xii ly nude thai va san xuat diesel sinh hgc.
II. PHUONG PHAP NGHIEN CUU 2.L PhiroTig phap thu man
M I U tao va mau nude cua hd Vinh Tan (thanh pbd Vinh, Nghe An) dugc thu Cling liic tai 3 diem (diem nhan nude thai vao, diem giiia hd va diem thoat nude thai ra) Email: [email protected] vn (N. L. A, VTnh)
N.LA. VTnh, P. T.N. Quynh, L. T. V Anh. T. T. H. An / Phdn lap vd tuyin chpn mpt sd chiing tao luc.
vao thang 7 nam 2015. Miu nude dugc lay d do sau 20 cm kl tir mat nude. Mau tao dugc vdt bang ludi thuc vat ndi co dudng kinh mit ludi 25 iim.
2.2. Phuong phap phSn tich chit lupng nirdc
Mdt sd chi tieu thiiy ly, tbuy hda dugc pban tich dua tbeo Quy chuan ky tbuat qudc gia ve chat lugng nude mat [4]. pH dugc do true tiep tai hien trudng bang may do pH. Oxy hda tan (DO) va nhu cau oxy hda sinh hgc (BOD5) dugc xac dinh theo phuong phap Winkler. Nhu can oxy hda bpc (COD) - Phuong phap kalibicromat. Amoni (NH4^ - Phuong phap so mau vdi tbudc thir Nesler. Photpbat (PO4"'") - Phuong phap so mau amonmolipdat. Chit rin la liing (TSS) - Phuang phap loc va can frgng lugng bang giay Igc dinh lugng.
2.3. Phuong phap quan sat va dinh loai loai tao luc
Cac tl bao tao luc dugc quan sat, md ta va do kich tbudc dudi kinh biSn vi quang hpc cd gin camera ket ndi may tinb va phin mem Motic Images Plus 2.0. Cac loai tao luc dugc dinb loai va sip xIp danh muc tbeo tai lieu pban loai cua Duong Diic Tien va Vo Hanh (1997) [6]. Mdi mau dugc lam 10 tieu ban; nhiing loai gap trong 8 den 10 tieu ban dugc quy uac la muc do gap nhieu (+++), 4 den 7 tieu ban dugc xem la miic dp gap tmng binh (++) va 1 din 3 tieu ban la mire do g^p it (+).
2.4. Phu-oug phap phan lap cac chiing tao luc
Mau tao thu tir bd Vinh Tan dugc nudi de tang sd lugng te bao bang each lay 150 mL mlu vao binh tam giac dung tich 500 mL, them 150 mL mdi trudng AF-6 [1], sue khi, dat dudi anb sang den neon vdi cudng dd chieu sang 3000 lux d nbiet dp phdng (25 - 30°C) trong 3 tuan. Lay 50 |j,L mau cay trai len dia thacb chiia mdi tradng AF-6; moi mau dugc cay len 6 dia va dat d dieu kien nudi nbu fren. Kbi kbuan lac tao luc phat frien dat dudng kinh tii 0,5 din 1 mm, chpn nbimg kbuan lac tach biet cay ria len cac dia tbach mdi. Cac kbuan lac tiep fric dugc lua chpn de quan sat dudi kinh hien vi; but nia te bao bang pipet Pasteur thiiy tinh; mdi te bao ho^ic mdi tap doan sau khi hiit nia dugc chuyen sang mpt gieng ciia vi dia 96 gieng cbiia mdi tradng AF-6 va dat d dieu kien nudi nbu fren [I]. Sau do, te bao frong mdi gieng dugc chuyen sang nudi va bao quan trong mgt ong ngbiem cd mit nhya cbiia 10 mL mdi trudng AF-6; mdi dng ngbiem dugc xem la mdt ddng te bao hay mdt chung. Cac chung dugc quan sat va djnh loai.
2.5. Phuong phap nghiSn ciru sinh t r u ^ g cua cac chiing tao luc - Phuang phdp xdc dinh mat do ti bdo: Sd lugng te bao tai thdi diem xac dinh dugc dim tren budng dim hdng ciu va mat dp tl bao dugc tinb toan dua theo tai lieu cua Andersen R. A. (2005) [1].
- Phuang phdp xdc dinh sinh khoi kho: Sinh khdi te bao tai thdi diem xac dinh dugc thu hdi bing phuong phap li tam (6000 vdng/phut, 10 phiit), say khd d 60°C cho den khi frgng lugng khdng ddi (khoang 8 gid), can sinh khdi kbd bing can phan ticb.
78
Tnrang Dai hpc Vinh Tap chi khoa hpc. Tgp 45, S6 3A (2016). tr 77-87 2.6. Phirong phap nghien cihi su* tich liiy lipid ciia cac chung tao luc - Phuong phdp tham do su hinh thanh lipid bang hinh dnh: Dung pipet hiit 1 mL mau tao vao dng nghiem Eppendorf dung ticb 1,5 mL; them vao 5 fiL dung dich Nile Red (0,5 mg/l00 mL ethanol); lac deu va giu trong tdi 20 pbiit. Ong nghiem dugc li tam vdi tdc do 4000 vdng/phiit trong 5 phiit. Miu tao dugc tach ra, lam tieu ban va chup anb bang kinh hien vi buynh quang Meiji Totechno vdi cbe dp anh sang thudng va chi dp anb sang mau xanh blue. Neu te bao cd chira cac gipt lipid thi binb anh hiln tiii bing mau vang va cac thanh phan khac la mau den.
- Phuang phdp chiit lipid tong so: Lipid tdng so dugc chiit xuit tir sinh khdi khd bang hdn bgp chloroform va methanol vdi ty le the ticb 2:1 dya tbeo phuong phap cua Bligh & Dier (1959) [3]. Djch chiet dugc cd dac bing may cd quay chan khdng va lam khd d nhiet dp 60°C den trgng lugng khdng ddi de xac djnb khdi lugng lipid thu dugc.
2.7. B 6 tri thi nghiem
- Thi nghiem doi vai cdc chung tdo Ivc duac phdn lap: Lay 30 mL gidng sau 10 ngay nudi ciia mdi chiing cbo vao binb tam giac dung ticb 500 mL cd chiia 300 mL mdi trudng AF-6 va dung mit day bing silicon cd 6ng dan kbi. Thi nghiem lap lai 3 lin dii vdi mdi chiing. Cac binh tam giac dugc sue khi lien tiic bang may tbdi kbi be ca; cbu ky chieu sang 12 gid sang : 12 gid tdi; cudng dp anh sang 3000 lux duoc cung cap bang den neon; dat d nbiet dp phong (25-30°C). Sau 16 ngay nudi, toan bd tl bao trong mdi binh dugc thu hdi de xac djnh sinh khdi khd, ddng tbdi tien banh tham dd kba nang sinb lipid cua chung.
- Thi nghiem doi vai mgt so chiing tdo liic dugc tuyin chon: Tbi nghiem dugc thiet ke tuong tu nhu tren vdi binh cau 5000 mL cd chiia 3000 mL mdi tradng AF-6 va dimg niit day cao su cd dng din khi, ty le gidng 10%. Mat dp te bao ciia mdi chiing dugc xac dinh sau khi nudi 0, 2, 4, 6, 8, 10, 12, 14 va 16 ngay. Kha nang sinh lipid dugc tham do bang hinb anh sau 10, 12, 14 va 16 ngay nudi.
- Thi nghiem nuoi sinh khoi di phdn tich hdm luong lipid tong so: De cd dugc lugng sinh khdi dii nhieu cho viec chiet lipid, cbiing tao dugc nudi frong 2 binh can dung tich 5000 mL tuong ty nhu tren. Sau 14 ngay nudi, dich nudi dugc ngimg sue khi, de lang trong 24 gid, hut bdt phan nude trong phia tren. Phan djch nudi dam dac dugc ii tam, say khd, tien banh chiet lipid va tinh toan ty le lipid tbu dugc so vdi sinh kbdi khd te bao.
III. KET QUA NGHIEN CUU VA THAO LUAN
3.1. Chat lircmg nirdc va thanh phan loai tao luc d ho Vinh Tan
Ket qua phan tich mdt sd chi tieu thiiy ly, thiiy hda ciia nude hd Vinh Tan vao thang 7 nam 2015 cho thay gia tri cac chi tieu vugt qua hoac khdng dat tieu cbuan ciia nude loai B| (QCVN 08:2008/BTNMT) diing cbo muc dich tudi tieu thiiy Igi hoac cac muc dich su dung khac cd yeu cau cbat lugng nude tugng tir (Bang I). Gia tri DO thap ban tieu chuan cho phep, BOD5 cao ban hai lan, COD cao hon ba lan; nhiing sd lieu nay chimg td nude hd Vinh Tan da bi d nhilm huu co. Ngoai ra, nude bd Vinh Tan con bi 6 nhilm amoni vdi ham lugng N H / cao bon bay lan tieu cbuan cho phep.
K L. A. ^rjh, P. r. N. Quynh, L T. V Anh, T.T. H An /Phan lap va tuyin chon mot s6 chiing tao luc
Bang 1. Mot so chi tieu chat lucmg mcac ho Vinh Tan vao thdng 7 nam 2015 TT
1 2 3 4 5 6 7
Chi tieu
pH DO BOD5
COD NH4*
P O 4 ' ' TSS
Don vi
mg O2/L mg O2/L mg O2/L mg/L mg/L mg/L
Ket qua phan ticll
7,0 ± 0,2 3,22 + 0,06 33,67 ±1,25 93,33 ± 38,89
3,63 ±0,12 0.41+0,06 76,8 ± 2,2
QCVN 08:2008/BTNMT (Cot Bl*)
5 , 5 - 9
> 4 15 30 0,5 0,3 50
Chu thich: * Cot B[ - Dung cho muc dich tuai tieu thuy Igi hoac cac muc dich su dung khac co yeu cau chat luong nuorc tuong tir.
Dong thai voi vi$c phan tich chat Itrong nuoc, chung toi da phan tich mlu tao thu tu nuac h6 Vinh Tan va chi gap 14 loai/duoi loai tao luc thuoc 6 chi {Chlorococcum, PediastiTttn. Chlorella. Coelaslrum, Scenedesmus va Ankistrodesmus) cua bo Chlorococcales {Bang 2), trong do, khong co loai nao 6 muc dp gap nhieu. Nhiing loai va dual loai gap o miic do trung binh bao gom: Chlorococcum infitsionum. Chlorella pyrenoidosa var. pyrenoidosa, Chlorella vulgaris var. vulgaris, Scenedesmus obliquus var. obliquus va Scenedesmus quadricauda var. quadricauda. Lien he vai ket qua phan tich chat lugng nuac ho Vinh Tan cho thay nhiing loai va dual loai nay co kha nang thich nghi vai moi truong nuoc thai do thi co 6 nhiem huu ca va ham lugng amoni cao.
Chlorella va Scenedesmus da dugc danh gia la hai trong muoi chi vi tao co kha nang chong chiu tot nhat vdi 6 nhiem hiiu ca of miic do 6 nhiem trung binh den 6 nhiem nang [2].
Bang 2. Thanh phdn loai tdo luc gap d ho Vinh Tdn vao thdng 7 nam 2015
TT Taxon MiJC d o g a p
Nganh Chloropbyta Ldp Protococcophyceae Bd Chlorococcales Hg Chlorococcaceae
1 Chlorococcum infusionum (Schrank) Menegh. ++
Ho Hydrodictyaceae 2
3 4 5
Pediastrum duplex Meyen var. duplex
Pediastnim simplex var. duodenarium (Bailey) Rabenb.
Pediastrum simplex vax.ovatum (Ehr.) Ergashev Pediastrum tetras (Ehr.) Ralfs var. tetras
+ + + + Hg Oocystaceae
Trudng Dai hoc Vmh ochikhoahpc, Tgp45.Sd3A(20l6).tr. 77-87
TT 6 7
Taxon Chlorella pyrenoidosa Chick, var. pyrenoidosa Chlorella vulgaris Beijer.-var. vulgaris
Miic dd gap -H- ++
Ho Coelasfraceae
8 Coelastrummicroporum Naeg. +
Hp Scenedesmaceae 9
10 11 12 13
Scenedesmus bijugatus (Turp.) Kuet. var. bijugatus Scenedesmus obliquus (Turp.) Kuet. var. obliquus Scenedesmus quadricauda (Turp.) Brebis. var. quadricauda Scenedesmus acuminatus (Lagerh.) Cbod. var. acuminatus Scenedesmus acuminatus var. maximus (Uherko.) Ergashev
+ ++
-H- + + Hp Ankisfrodesmaceae
14 Ankistrodesmus gracilis (Reinsch.) Korscbik. + Chu thich: + mire dp gap it; ++ mfrc dd gap tmng binh; +++ miic dp gap nhieu
3.2. PhSn 13p va tuyin chon mot so chiing t^o luc giau lipid tir nude ho Vinh TSn
Tam chung tao luc thudc 3 trong sd 5 loai cd mire dp gap cao nhat (miic dp trung binb) trong nude hd Vinb Tan da dugc phan lap tbanh cdng. Kich thudc va hinh thai cac tl bao cua mdi chung la tuong ty nhau (Bang 3 va Hinh 2 - Cot A) chimg td tat ca cac te bao frong mdi chung dugc pban lap la cdng mdt loai. Cac chiing dugc dat ten va dinh loai loai nhu sau: chiing VT-4D, VT-5B va VT-6C thudc loai Scenedesmusobliquus; VT-IA, VT-3C va VT-5C tbudc loai Scenedesmus quadricauda; VT-4C va VT-5D tbudc loai Chlorella pyrenoidosa.
Bang 3. Kich thuac ti bdo ciia 8 chiing tdo luc duac phdn lap TT
1 2 3 4 5 6 7 8
Ten chiing VT-4D VT-5B VT-6C VT-IA VT-3C VT-5C VT-4C VT-5D
Ten loii
Scenedesmus obliquus Scenedesmus obliquus Scenedesmus obliquus Scenedesmus quadricauda Scenedesmus quadricauda Scenedesmus quadricauda Chlorella pyrenoidosa Chlorella pyrenoidosa
Kich thu-6'c te bao (chieu dai x chieu rong) ^im
(6,4 - 9,4) X (2,5-5,0) (5,0 - 7,0) X (2,2-3,4) (5,1 - 6,2) X (2,2-2,9) (8,8 - 11,2) X (2,9-4,4) (8,3 - 10,2) X (3,7-5,3) (10,4 - 16,6) X (4,0-8,4)
(4,2 - 8,2) X (2,4-6,2) (4,2 - 8,2) X (2,4 - 6,2) Sy sinh tiudng ciia 8 chiing nay dugc danh gia nhanb bang nang suat sinb khdi khd sau 16 ngay nudi frong mdi trudng AF-6 va dugc trinh bay d Hinh I. Chung VT-4D cbo
N. L A. Vmh. P T.N. Quynh. L. T. V. Anh, T. T. H. An /Phan Igp vd tuyin chon mpt s6 chimg tdo luc..
sinb khdi khd thip nhit (0,679 g/L). Sinh khdi khd cua cac cbiing edn lai khac nhau khdng ddng kl, tbay ddi tir 0,848±0,04 g/L (chung VT-5B) din 0,98+0,06 g/L (cbung VT-5C). Trong nghien cim trudc day, nang suit tdi da ciia Scenedesmus cd the dat 1,2 g/L [9].
Hinh I. Sinh khoi cua 8 chung tdo luc sau 16 ngay nuoi trong 300 mL moi trudng AF-6 Ket qua nhugm te bao vdi Nile red va chyp anh biSn vi dudi anh sang buynh quang xanh blue cho thiy hinh anb te bao cua 8 cbiing deu xuat hien nhiing viing mau vang trong te bao (bieu tbi bdi mau fring ddi vdi anh den trang) (Hinh 2, cot B), cbiing to tit ca 8 cbiing diu cd kha nang binh tbanb lipid nhung vdi nhiing bam lugng khac nhau.
Trong sd 6 chiing Scenedesmus, lipid d cac chiing VT-4D, VT-5B, VT-6C va VT-IA chu ylu tap tmng d hai dau tl bao; frong kbi do cbiing VT-3C va VT-5C kbdng cbi hinb tbanb gipt lipid d hai dau te bao nbu nhiing cbiing khac ma con hinb tbanb d phan giiia te bao. Trong mdi cbiing Scenedesmus, ham lugng lipid dugc hinh tbanh d cac te bao tuong ddi ddng deu sau 16 ngay nudi. Hai cbiing Chlorella pyrenoidosa ciing hinh tbanb nhiing gigt lipid nhd frong te bao; my nhien, hinh anh cbo tbay bam lugng lipid cua cbiing van con thap sau 16 ngay nudi.
Dya tren cac tieu chi ve kha nang sinh lipid, kich thudc te bao va nang suat sinh khdi, cbiing VT-3C va VT-5C dugc danh gia cao nhat frong sd 8 cbirng nghien ciiu bdi vi cbiing cd kha nang sinh lipid tdt nhat (Hinh 2), te bao cua hai chiing nay cd kich thudc Idn nbat (Bang 3) nen thuan Igi ban frong viec thu hdi sinh khdi, ddng tbdi nang suat sinh kbdi cua chiing cung kbdng thap ban nhiing chung edn lai (Hinh 1).
Trucmg Dai hoc Vinh
A
rcip cAi AAod hpc. Tap 45. Sd 3A (2016,
B
). Ir. 77-87
Chiing:
vr-4D
VT-4C
Hinh 2. /TinA a«/i A/en v/ ciia 8 chung tdo luc .\ciu 16 ngay nuoi duoc nhi/oui voi Nile red.
Cot A - Cdc chung duac chup dudi dnh sdng trdng. Cot B - Chup dual dnh sdng huynh quang xanh blue; cdc gigt lipid biiu thi bdi viing mdu trdng trong te bdo doi vdi dnh den
trdng hoac mdu vdng ddi vdi dnh mdu. Thuoc tyli- 20 pm.
N.L A Vinh. P. T. N Quynh. L. T. V. Anh. T. T. H. An /Phdn tap va tuyin chpn mpt so chimg tdo luc...
3.3. Dong hpc cua sir sinh tnr&ng va tich luy lipid cua chung VT-3C \k VT-5C A B ^ ^ ^ ^ ^ ^
Ngay 10
Ngay 12
Ngay 14
Ngay 16
t^^^ * • * * » *
•
Ngay12
Ngay 14
Ngay 16
Hinh 3. Anh hiin vi thi hien su tong hop lipid cua chung VT-3C vd VT-SCsau 10, 12, 14 vd 16 ngay nuoi. Cot A - Cdc chung duoc chup dual dnh sdng trdng. Cot B - Chup dual
dnh sdng huynh quang xanh blue voi thuoc nhugm Nile red; cdc giot lipid bleu thi boi
TnrdngDgihpc Vinh Tgp chi khoa hpc. Tdp45. So 3A (2016). tr. 77-87 vung mau trdng trong ti bdo doi vdi dnh den trdng hogc mau vdng doi voi dnh mdu.
Thudc ty li: 20 pm.
B • ' 35
§ ^ S21 1 2 l i s
1 - 05
4 *
% —\
l^j_053!
ill
I fliChOngVT-3C
! aCfiOngW-SC
Hinh 4. (A) Sif sinh truong ciia chung VT-3C vd VT-5C trong 3000 mL moi trudng AF-6 vd(B)hdm luong lipid so voi sinh khoi kho cua chung sau 14 ngay nuoi
Hinh anh t l bao nhugm Nile red dudi anh sang huynh quang xanh blue sau 10, 12, 14 va 16 ngay nudi cho thay chung VT-3C va VT-5C deu dat dugc nang suat lipid cao nhit bat dau tir ngay thii 14 (Hinh 3). Ben canh dd, ket qua nghien ciiu sinb tmdng dya vao mat do te bao cho thay do tbi sinh trudng cua chiing VT-3C va VT-5C la tuong ty nhau (Hinh 4'A). Pba sinh tiirdng logarit dugc xac dinh la tir ngay thii 2 den ngay tbii 10 (tbdi gian dl tang gap ddi so lugng t l bao ciia cbung VT-3C la 40,85 gid, cua chung VT- 5C la 49,35 gid). Pha can bing la tir ngay thii 10 din ngay tbii 16; mat do te bao cua chung VT-3C va VT-5C dat cao nhit vao ngay thu 14, tijcmg iing la 138,39 va 138,42 frieu tl bao/mL. Pha suy vong dugc xac djnb la tir ngay tbii 16; tbi bien bdi mat do tl bao giam din, mau sic sinh kbdi chuyln sang xanh tdi va van dye (sd lieu khdng trinh bay); tuy nhien, t l bao sdng frong dich nudi van cd the duy tri den ngay tbu 60.
Lien he giua su sinb trudng va sy tao tbanh lipid ciia chiing VT-3C va VT-5C cho thiy tbdi dilm thu hoach cua ca 2 chiing vao ngay tbii 14 (giiia pba can bing) la dat hieu qua cao nhit ca vl sinh khdi va ham lugng Hpid._ Tbeo Yoshida et al. (2010) [12], trong dilu kien mdi trudng ngbeo dinh dudng nhu thieu ngudn nita thi vi tao se tbay ddi con dudng tdng hgp lipid va bit diu ticb liiy friacyl glycerols (TAGs); mat khac TAGs khdn^
dugc su dyng dl tdng bgp ciu tnic mang n6n dugc tich luy thanh cac gigt lipid frong te bao chit. Kit qua tach chiit lipid tir sinh khdi khd sau 14 ngay nudi cua 2 chiing dugc trinh bay d Hinh 4-B. Tbeo dd, ty le lipid ciia cbung VT-3C la 28,4% sinh kbdi khd va ciia chung VT-5C la 26,3% sinh khdi khd. Trong cac ngbien ciiu tru'dc day, ham lugng lipid ciia mpt sd chiing Scenedesmus dat tir 12,7% den 43% sinh khdi khd tiiy tbeo dieu kien mdi frudng nudi khac nhau [7, 8].
KET LUAN
- Mudi bdn loai/dudi loai tao luc thudc cac chi Chlorococcum, Pediastrum, Chlorella, Coelastrum, Scenedesmus va Ankistrodesmus dugc tim thiy trong ngudn nude hi d nhilm huu co va d nhilm amoni ciia hd Vinh Tan (thanh pho Vinh, Nghe An).
N L. A. Vinh. P. T. N. Quynh, L. T. V Anh. T. T. H. An /Phdn idp va tuyin chon mot so chiing tdo lye...
- Tam chiing tao luc thudc chi Chlorella va Scenedesmus da dugc phan lap tii nude hd Vinh Tan, frong dd, chung Scenedesmus quadricauda VT-3C va S. quadricauda VT-5C cd kha nang tich luy lipid tdt nhit. Thdi gian nudi ngin nhit dl dat dugc mat dp te bao va bam lugng lipid cao nhat la sau 14 ngay nudi frong mdi trudng AF-6; theo dd, mat dp tl bao cua chung VT-3C va VT-5C tirong img la 138,39 va 138,42 tiieu tl bao/mL; ty le lipid ciia cbiing VT-3C va VT-5C dat tiiang iing la 28,4 % va 26,3 % sinh kbdi kbd.
TAI LIEU THAM KHAO
[I] Andersen R. A. (Ed.), Algal culturing techniques, Elsevier Academic Press, 2005, pp.
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Tnrang Dgi hpc Vinh Tap chi khoa hpc. Tdp 45. So 3A (2016). tr. 77-87
SUMMARY
ISOLATION AND SELECTION OF LIPID-RICH STRAINS OF THE GREEN ALGAE IN VINH TAN MUNICIPAL WASTEWATER RESERVOIR
(VINH CITY, NGHE AN)
Vinh Tan reservoir (Vinh City, Nghe An Province) is one of places receving the municipal wastewater of Vinb City. Our investigation indicated that it was polluted with organic substances and ammonium. For the Chlorophytaphylum, 14 species and subspecies were found in Vinh Tan reservoir. Eight sfrains of the following species:
Scenedesmus obliquus (3 strains), S. quadricauda (3 sfrains) and Chlorella pyrenoidosa (2 sfrains) have successfully been isolated. Of these, tbe sfrains S. quadricauda VT-3C and S. quadricauda VT-5C showed the best abilities of lipid accumulation. In AF-6 medium, tbe shortest time of cultivation to obtain the highest cell densities and lipid contents was at the day 14 of tbe growth; accordingly, the cell densities of the sfrains VT-3C and VT-5C were 138.39xloSnd 138.42x10^cells/mL; tbe lipid contents of tiie sframs VT-3C and VT-5C were 28.4% va 26.3% of dried biomass, respectively.
Loi cdm an: Cong trinh nghien cuu nay dug^c tdi tra bdi de tdi T2016-37 cua Trudng Dgi hoc Vinh.
