Tap chi Cong nghe Smh hpc 10(2) 247-253,2012
D A H i N H M O T S O C N G G E N L I E N Q U A N D E N C H A T L U Q N G T H I T Of LgfN Y O R K S H I R E V A M O N G C A I
Nguyin Thi T h u ' , Vii T r g n g Lu•g•ng^ T r a n Xuan Hoiin'. N}iii>cn Van Cu6-ng' ' Vien Cong nghe sinh hgc. Vien Khoa hpc vd Cong nghe ' 'lei ^ am
'Truang Dai hoc Sir pham. Dgi hpc Thai Nguyen Vien Chan nuoi. Bp Nong nghiep vd Phai Irien Song tluhi
TOM T A T
Viec su dung cac con lai giiJa Ign ngojii vdi Ign npi da nang cao nang suat nganh chan nuoi Ig-n, luy nhien chal lugng thil cua Ign lai thuong khong biing Ipn npi Myc dich cua nghien cuu nay phan lich da hinh cac ung gen lien quan chat lugng ihu a 2 giong Ign ngogi \a ngi phyc vg phiii tnen chi th| phan lu ho trg cong t^c chgn Igc Trong nghien ciru nay da hing 3 img gen Lpin\. H-FABP va MC4R lien quan chat lugng ihjt dugc phan tich bang phuong phap PCR-RFLP o 56 mau Ign Yorkshire \a 94 mlu Ign Mong Cai Kel qua cho thSy gen Lpin\ CO tinh da hinh o ca 2 giong Ign Tansoatelle C cho chat lugng ihji lot o Ign Yorkshire \a Mong Cai lan lugt la 0,71 \a 0.995 Tuy nhien dgdi hgp lu (He) o liai giong Ign Yoikshiie \a Mong Cai la khac nhau co y nghia, lan lugt la 0,41 va 0.01 Phan tich RFLP hai gen H-FABP va MC4R o Ign Yorkshire cho tliiy tai 2 locus tren co tinh da hinh. Tan so alelle lien quan ham lugng md giSi cao a si A a gen H-FABP va MC4R lan luot la 0.13 va 0,85. 0 Ign Mong Cai. kin qua phan tich cho thay tat ca ca the deu mang dong hgp Xa AA a locus H-FABP va GG o locus MC4R Dg di hgp tu (He) cua 2 gen H-FABP va MC4R a Ign Vor"kshire i k lugt la 0,23 vaO.26 Ham lugng thong tin da hinh (PIC) cua 3 genl;)»jl, W-F^BP va .WC^/? g Ign Yorkshire l b lugt la 0.33; 0.21 \a 0.22 Doi \<ji Ion Mong Cai chi so PIC ciia gen H-FABP sa MC4R bang khong, con gen LpinX la 0.001. Ket quacho thay tinh da hinh 3 ungeen tren a Ion Yorkshire cao hem 1cm Mong Cai. Viec xac dinh su lien quan giOa kieu gen \ oi chat lugng thii tiep tge phan tich cae the he con lai cua hai giong tren Tu khoa: chdl luanglhit. H-FABP. Lpinl, \IC4R Mong Cai. Yoik\hi,e
M O D A U
Cac giong Ion noi Viet Nam thuang cho chat hrpng thit tot, ngon khi che bien, song nhuoc diem la tang trircmg cham, trong luang thap, thanh phan ma nhieu trong thjt xe \ a ty le tieu ton thirc &n cao.
Trong nhiing thap men qua viec sir dung cac con lai giiia lan ngoai va Ign npi da nang cao toe dp smh truang, ty le nac Ion; song thit lan lai thuang tra nen dai hon, khong co vi thom nhu Ion npi (Thien et al, 1996), Nghien cim chpn Ipc ^i6ng Ipn cho nSng suat cao nhimg vln giO duoc chat lugng thit ngon co y nghia quan trpng trong san xuat. dap img nhu cau ciia nguoi tieu dimg. Danh gia mire dp lien quan giiia da hinh gen vai ch§t lupng thit la mot trong nhiing huong CO nhieu trien vpng ho trp chpn Ipc nhiing giong biing chi thi phan tii. Den nay nhieu gen lien quan den chat lugng thit, trong do co cac gen Lipin 1.
H-FABP, MC4R da dugc xac dinh (Peterfy et al, 2001; Gerbens et ai, 1998; Huang M et ai. 2010).
G H I LpinX, nam a nhiem sac the so 3 t^i vi tri 3q21-q27, la mpt ling gen lien quan ham lirpng m o giat 6 thit Ion (He et ai. 2009). O nguai sv bieu hien
cao gen nay dan den gan nhiem mo. tang triglyceride mail, va khang insulin. 0 Ion, gen nay dong vai tro trong viec phat trien binh thuang md mo va la nhan to anh huong den sy tich trir mg trong thit Ipn (Peterfy et ai. 2001). Theo ket qua nghien cim ciia Zhao va dong tac gia (2009) \ e gen Lpinl tren 162 con Ion thuoc giong Tong Cheng, Large White \ a cac con lai, kieu gen CC c6 ham lupng m5 la va mo giat thap han so vai lan co kieu gen TT va TC Lgn mang kieu gen CC cho chat luong thit tot hon 2 kieu gen con lai do ham lirgng ma co thich hgp, thit mem ngon khi che bien.
Gen ma hoa protein lien ket acid beo a tim (H- FABP), nam tren nhiem sac the so 6 a Ipn tai vi tri 6q2l-q26, ma hoa cho protein gk\ axit beo o ca (Gerbens et al, 1996). Gen H-FABP va A-FABP dirge coi la hai ling cir gen cho tinh trang ham lirpng md ca a Ign (Zhao el al. 2009). Tuy nhien su tuong quan giiia cac kieu gen H-FABP voi ham lirpng mo ca a cac giong Ign khac nhau la khong giong nhau.
Lgn Duroc kieu gen aa co ham lugng m o co cao hon Ipn mang kieu gen AA. Trong khi do a lan Pietrain kieu gen AA co ham lugng m a co cao hon aa 247
Nguyen Thj Thuetal (Gabcns el al. IW'M
Gen thy the melanotoiiin-4 {\IC4R) nam Iren nhiem sJic thi- so I o Ign l^i v] tri Iq22-q27 (Kiiii el ai. 2000) MC4R 1^ img gen lien quan sy phA( irien dp day ini> linig \ a ham lupng mo giiii trong thjt.
Ngoai ra gen MC4R ton thain gia vao vice kicin so,il lupng thirc ."in. neu hao naiii^ lugng va liic dp lang iruong Ipn (Ho.mc et ai. 20111) Kel qua nghien curu ciia Piorkowsk.i \a dong i.ic gi.i (2010) tren mpt so giong Ipn 1 arge W lute. I andracc, I'lil.iuska. Piclrain
\ a Duroc cho ih.-iv kieu gen (-(' cho ham lugng mO iziai thap ban hai kieu gen con l^i la .\.\ \;'i AG
Ci \'iel Nam nghien ciru \ e cac gen hen quan chat lugng thit lt,Tn dugc quan tam nhicu trong nhiing nam g£in da> Da hinh //,/<ll-R! LP eua gen H-FABP a 5 giong Ipn dupe de eiip den trong tong irinli cua Nguyen Thu Thuy \ a dong lie gia (200'^) Kel qua cho thay a lgn n(n chi \u,"ii hi?n 2 kieu gen DD va Dd. Tan suat kicu gen DD a 5 giiiiig Km npi (Tgp Na, Mong Cai, Co. Muong Khuong, Mi,-o| cao han nhii'u so \cTi kieu gen Dd. T? Th| Thoa va dong tac gia (2009) dS Uen hanh phan tich danh gid miic dp lien quan giCia haplotype ciia gen H-FABP \ a t toe dp sinh truong va chat lupng thit Ign. Ham lupng md trong CO tuong img cac kieu gen DD. Dd. dd lan lugt la 2.27"o. 2.49"'o \ a 2.91%. Trong bai nay, tan suat alelle. dg di hpp t u cac gen Lpml. H-FABP. MC4R a 2 giong Ign Mong Cai va Yorkshire dupe phan tich, so sanh va danh gia. Muc dich xac dinh dugc kieu gen lgn giong bd m? cho dan lai tftng truong nhanh, chat lugng thjt lot.
phan phan img PCR cho limg img gen dugc trinh biiy bang 2,
(•///( uinh nhii'l. Truac het bi&n tinh DNA khuon 94"C (4 phut) sau do lien hinh 35 chu trinh khuygch d^i DNA nhu sau, bien tinh DNA 94"C (30 giay), u bfit c$p m6i (iO"C (40 giay). long hgp keo dai chuoi 72"C (4'i giiiy), Phan img ket thiic 6 72"C trong 7 phiil San ph^in PCR dugc giii o 4"(
Phirmic phiip RFLP
Ihanh phan phan img RFLP gom; san pham PCR 18 pi. enzyme (Tap\. \!sp\} 2 pi. buffer 5 pL H;0 khii ion 3 pi, Phan img dugc thyc hi?n qua dem 6 W"( San pham cat dugc kiem tra tren di?n di gel agarose 2.5% ( ac thdng tin lien quan enzyme ck da hinh RFLP, alelle. kich Ihu6c cac dogn da hinh cua cac gen Lpinl. HFABP. \IC4R th^ hi?n bang 1.
Phyanf* phdp thong ke
Tan so kieu gen. lAr so alelle dupe tinh tryc tiep lir ket qua phan tich RFLP Mut dp sai khac \e tin so cac kieu gen va alelle tren hai giong nghien cuu dupe xac djnh b9ng phep thu Chi binh phuong (x-l theo Takuya va dong tac gia (1999). Can bang di truyen quan the Hardy-Weinberg dugc \ac djnh bJng chuong trinh phan mem BlOSYS-2. Muc dg da hinh dl truyen dugc \ac djnh dya tren dp di hgp tir mong d g i - ( H e ) theo Nei (1978).
VAT LI$U VA PHUONG PHAP Vat li^u
Cac mau Ipn Yorkshire (n = 56) dugc lay tir tr^i thi nghi?m TKgy Phucmg (Vi^n chSn nuoi). mau Ign Mong Cai (n - 94) dugc lay tir dim lgn thuan ciia cong ty Thanh To, Hai Phong. Cac mau mo dugc lay tir lai lgn (Ig), bao quan ttong c6n 75". a -20"C, Cac cSp moi dupe sir dgng trong nghien cthi nay dugc long hgp bai hang Fermentas (Dire) va c6 trinh ty nhu bang 1.
Phirong p h ^ p khuyech dai DNA b J n g PCR Dieu kien PCR. DNA h? gen su dyng trong PCR dugc tach chiet tir cac mau mo tai theo Nguyen Thu Thuy va dong tac gia (2005). Chat lugng DNA dupe danh gia bang di$n di tren gel agarose 1%. Thanh
p, la tan suat ciia alelle thir i trong tong so K alelle
Ham lugng thong tin da hinh - polymorjAism information content (PIC) dugc tinh theo cong thiic:
n P I C i - 1 . I : P I J 2
ri
PlCi la ham lugng thong tin da hinh o locus i;
Pij la tSn so cua alelle thii j trtwig tap hgp n alelle l?i locus i.
So sanh hai ty 1? xdc suat dugc kiem djnh bJng Z - Test. V^ ca ban, Z-Test gi6ng nhu T - Test dugc sir dyng de so sanh hai ty 1^ xac suat va dugc tinh theo cong thirc:
N {obs, - exp.y exp^
Tap chi Cong nghe Sinh hpc 1 0 ( 2 ) : 2 4 7 - 2 5 3 . 2 0 1 2
z = ( p i - p : ) / S E
Trong do p i la ty le xac su5t nh6m I. p2 la ly Ig xac suat nhom 2. SE la dp lech chu3n ciia phan ph6i mau,
Gia tri Chi binh phuong (x2) danh gi^ can Trong do: «/w, = so c^ the quan sat dugc a kieu bang Hardy-Weinberg dupe xac djnh theo cong gen i; e.\p, = so ca the dg dodn theo tinh to^n 6 kieu thirc: gen i.
Bang 1 Trinh t u m6i v ^ da hinh PCR-RFLP cua cSc gen Lpml. HFABP. MC4R
Gen Trinh ty> mol San Enzyme Alelle Kich NguAn phdm c i t da thu'6^
PCR (bp) hinh do?n da hinh (bp) Gen Lpln^ F 5'-GTTTGTCACCGTGAAGGA-3
R.5'-AAGCCACAGTAATCAGAACA-3'
316 Taq 1 316
Gen F-5-ATTGCCTTCGGTGTGTTTGAG-3' 816 HFABP ^ 5-TCAGGAATGGGAGTTATTGG-3' gen F-5-TACCCTGACCATCTTGATTG-3' 220 '^^'*'^ R- 5'-ATAGCAACAGATGATCTCTTT-3'
Mspl a A 7"aq 1 A
G 816 727/89 220 150/70
Pang ef al. 2006 Kim el al.
Bang 2. Thanh phan phan ung PCR nhSn cSc doan gen Lpml, H-FABP va MC4R
1 nann pnan ^p,.„^
ti'ng ger H-FABP
i{Ml) MC4R HjO khi> ion
Buffer 10X MgCI; 25 mM dNTPs 25 mM Mfiixufii 10 pM M ^ i n g u ^ c 10 pM Taq 1 iil\i\ (Fermentas, Invitrogen) DNA khuon 50 ng/til Tdng the lich (gl)
KET QUA VA THAO LUAN Gen Lpinl
San phdm PCR cua gen Lpinl la doan nucleotide 316 bp n i m ttong exon 2 chila dpt bien C93T khong lam thay doi trinh ty amino acid (He et ai, 2009).
Phan tich PCR-RFLP gen Lpinl sii dyng enzyme cat giai han Taqlc\io alelle Ttuong img bang 316 bp va alelle C tuong ling voi san pham 256 va 60 bp (Hinh 1). Kat qua phan tich da hinh TaqI PCR RFLP gen LpinX tren 56 mau Ipn ngoai Yorkshire va 94 Ign
npi Mong Cai dupe trinh bay Bang 3
Ket qua phan tich cho thay o Ign ngoai Yorkshire xuat hien ca 3 kieu gen TT. TC va CC vai tan so tuong img la 0,07; 0,45 va 0.48. trong khi a lgn npi Mong Cai chi xuat hien 2 kieu gen TCva CC vai tan so tuong ung la 0,01 va 0,99, Tgi locus phan tich, can bang di truyen Hardy-Weinberg tim thay 6 ca hai giong lgn vai gia fri P,YI = 0,58: va P ,MC) - 0,96 Cac mau phan tich dugc cho la can bang di uuyen Hardly-Weinberg neu gia trj P > 0,05. 0 ca hai giong lgn tan so alelle C (lien quan ham lugng m a
Nguyen Thj Thuetal cao Irong co) eao han alelle T (P- (I.OI), (i Km
Mong Ciii tin so alelle C rat cao (().'»'>^).
Hinh 1 Da hinh TaqI PCR-RFLP gen Lpinl it Ign M marker gi^ng 1 s^n ph^m PCR, gi6ng 2 4. 9, 10. 11. 12, 13 ki4u gen CC, gi^ng 7 kiAu gen TT, gi4ng 3, 5, 6, B,14 ki4u gen TC
Kel qua nghien ciai ciia Zhao va dong tac gia (20(1')) lien lii2 l(,m ihupc cac giong, Tong Cheng.
I arge White v;i cac con lai cho thay lan so alelle C cao IHVII aleile T. Lgn mang kieu gen CC co ly I? mO i:ial ihap lnrii kieu gen CT va TT do vay cho chat liiimg ihfi loi (lie ('/<//. 2009). 0 nguai sy bieu hi?n t a o eua ^;eii Lpinl gay chirng beo phi, ham lupng md Irong CO cQng nhu lugng mft tich trii cao Ket qua pliaii iicli cho thay alelle "gay beo phi" 7" thap a ca 2 tjion^ lgn Kel qua nay phii hgp thyc te la 2 gi6ng iicii khong ihiipt giimg Ipn beo phi. Tan suat alelleC tan IT lgn Monj; ( .ii t o the do ket qua qua trinh chgn Ipt. VI nliOng l(,rii nhanh hco da bj thai lo^i. Dp di hpp tir mong dgi (He) va ham lugng thong tin da hinh (PK ) a Ign Yorkshire deu cao hon Ipn Mong ( ,11(1'- 0.01)
Bdng 3. TAn s4 ki^u gen. ki^u alelle v4 dd di hgp l u cua gen Lpinl ir Ign Yorkshire va M6ng Cai
S6 mSu (n) 56 f 94
TSn s6 kl6 (so miiu) TT
0,07 (4j 0 ( 0 )
TC
0,45 (25) 0,01(1)
CC
0,48(27) 0,99(93)
T i n s6 alelle (s6 lu-g-ng) T
0,29(33) 1 0,005(1) 1
0,71(79) 0.41 0,330 0.995(187) 0.01 0.001
He = 0 $ dl hop tir mong dpi - expected heterozygosity, PIC = HSm l u g n g thftng tin da hinh - polymorphism information content, -/2 - Ph6p thir chi binh phuong, ns = sai khac khflng c6 y nghia. Z-Tesl = So sdnh muc d$ sai k h ^ giira hai lyl$
xac su^l (n > 30), " ' = mi>c y nghia P- 0,01
Gen H-FABP
San pham PCR gen H-FABP la doan nucleotide CO kich ihuoc 816 bp (Pang et al, 2006). Da hinh RFLP gen H-FABP sir dgng enzyme cit .\l\pl cho alelle a tuong img bang 816 bp - san pli.im PCR khong bi cat boi M.vpl va alelle A tuang img voi hai bang 727 va 89 bp - san pham PCR b| cat bni .\lspl (Hinh 2).
Ham lugng mo ca la mpt trong y^u to quan trpng lien quan chat lugng thit nhu dg m^in, miii thorn cua thit. Nang cao chat lupng ihjt bang phucmg phap chpn igc truyen thong gap nhieu tro ngai vi phai xac djnh tryc tiep linh trgng cr thjt lgn mo, Su dyng chi thi phan tir khac phyc \ia nggi tren do cho phep chpn Ipc ngay khi lgn moi sinh va khong f)hai giit mo Gen H-FABP bi^u hi?n chii yeu trong te bao co \ a dugc xac djnh lien quan ham lupng ma ca, Tuy nhien kieu gen (alelle) cho chat luong thit tot khong giong nhau a cac giong Ipn
(Gerbens el al. 1999). KOt qua phan tich cho thay is lgn ngoai Yorkshire \u.it hien 2 kieu gen .-l.-l \a aa vai Kin so tuang img la ii.S".'^ va 0,125, trong khi o lgn npi Mong i .w thi .\uat hi^n 1 kieu gen AA (Bang 4). Sy phiin bo tan ^6 alelle H-FABP a lgn Yorkshire va Moiii; t ai la khac bi?t co y nghia (P< 0.01).
Hinh 2. Oa hinh Mspl PCR-RFLP gen H-FABP d Ign, M.
marker gi^ng V sSn phSm PCR, gi4ng 2,4,5,6,7,8,9,10:
ki^u gen AA: gt^ng 3, ki^u gen aa.
250
Tap chi Cong nghe Sinh hoc 10(2). 247-253, 2012
Bang 4 Tan s6 kieu gen, k i l u alelle vA dg dj hgp lii' ciia gen H-FABP a Ig'n Yorkshire u^ M6ng Cai
Giong lan S 6 m a u T i n s6 kieu gen (somSu) T i n so allele (s6 lu'p'ng) He PIC x2
Yorkshire Mong Cai Z-Tesl
56 94
0,875 (49) 1(94)
0 ( 0 ) 0 ( 0 )
0,125(7) 0 ( 0 )
0,875 (98) 1 (188)
0,125(14) 0(0)
0,23 0
0,21 0
He = Do di hop tii' mong dgi -expected heterozygosity, PIC= H^m lugng thftng tin da hinh . polymorphism information content, x2 = Phep thu' chi binh phuong, ' " = mire y nghia P< 0,01, Z-Tesl = So sSnh mu'c dd sai kh^c giO'a hai ly 1^ xac suat (n > 30), " * = muc y nghfa P-. 0.01
Kel qua nghien ciru ciia Pang \a diing lac gia (2006) tren cac giong lan Duroc, Large While, Landrace, Neijiaiig, Rongchang. Baniei, llanjiang Black, Hanzhong While va nipt so giong lgn dia phuong cho lha\ tan so alelle A km han alelle a va tan so kieu gen . i -I kin hon Ion so ciia 2 kieu gen Aa va aa. Dp di hop tir mong dgi (He) o lgn Yorkshire va Mong Cai lan lirgt la 0.22 va 0. ham luong ihong tin da hinh tuong img la 0,21 \ a 0. Ket qua thu dugc cho thay mire da hinh lai locus H-FABP o km Yorkshire cao ban Ion Mong Cai (P<0.01) Gen melanocorlin-4 receptor (MC4R)
San pham PCR gen MC4R la doan nucleotide c6 kich thuac 220 bp chua dot bien tha\ the G > A (Min et al. 2004) Phan tich RFLP gen MC4R sir dung enzjine cat TaqI cho alelle A tuong img bang kich thuac 220 bp va alelle G tuong img voi san pham 150 va 70 bp (Hinh 3) Da hinh §en .MC4R duoc xac djnh hen quan ty le nac va toe do smh truang a Ign, tuy nhien mire dp tac dpng ciia timg kieu gen len cac giong Ign la khac nhau (Kim el al.
2000). Kk qiia phan tich da hinh TaqlRFLP gen MC4R cho thay o lgn ngoai Yorkshire xuat hien 2 kieu gen AA va AG voi tin so tuong img la 0,70 va 0,30, trong khi a lgn npi Mong Cat chi xuat hien I kieu gen GG. Khong phat hien alelle A trong 94 mau lgn Mong Cai T§n s6 alelle A va alelle G cr lgn Yorkshire la 0,85 va 0,15, trong khi a Ipn MC tan so alelle G la 1,00 (Bang 5). TSn s6 ki6u gen trong cac mau Ign Yorkshire dat can bang Hardy Weinberg, trong khi Ion Mong cai khong xac dinh duac
Sy phan bo tan so alelle a 2 giong Ign gan nhu trai ngugc nhau K6t qua fren phii hgp nghien cuu ciia Kim va va d6ng tac gia (2000) lgn mang kieu gen GG c6 miic tang ypng thap hon kieu gen AA va AG. Tuy nhien k^t qua ciia chiing toi trai vai nhan dinh cua Piorkowska va d6ng tac gia (2010) alelle G CO tan so cao a cac giong lgn nac nhu Piettain la
92.4" (1, va en lan so Ihap IT tiic gidng Ign m6 Duroc la '^1,5%. Nguyen nhan gay nen su khiic biel co the do muc dich chpn loc Ipn Yorkshiie va Mong Cai la khac nhau dan den ban dial di Iruycn khong giong nhau
Kel qua \ac djnh dp di hop tir mong dai va ham luong ihong Un da hinh cho thay mire da hinh tai locus \tC4R a lgn Yorkshire cao lion Ion Mong Cai (P<0.01)
bp 1 2 3 4 5 6 7 8 9 M
^m mm :!*»'"
Hinh 3 Da hinh TaqI PCR RFLP gen IV1C4R o Ion M marker, gieng 1 kieu gen AA gieng 2 kieu gen AG, gieng 3,4,5,6,7,8,9 kieu gen GG
Phan tich da hinh RFLP ba gen lien quan chat luong thjt Lpm\, H-FAB vd MC4R cho thay co sy sai khac CO y nghia thong ke ve tan so kieu gen va tan so alelle giua hai giong lgn Yorkshire va Mong Cai, Doi vai gen Lpin\ va H-FAB. hai alelle tuong img la C va a deu chiem uu the a ca 2 giong lgn. Truimg hgp ciia gen MC4R. a lgn Mong Cai alelle G chi^m uu the so vo'i alelle A, ngugc lai 6 lgn Yorkshire alelle A Igi chiem uu the. Ket qua tren la ca sa du lieu quan trpng ban dau cho viec phat trien chi thi phan til ho trg chgn con lai co nang xuSt cao, ch4t lugng thit lot. Ket qua xac dinh He va PIC cho thdy mire dg da hinh ba img gen hen quan chat lugng thit a lan ngoai Yorkshire cao hon lan noi Mong Cai,
251
N g u y e n T h | T h u c V w / ,
B a n g 5 T i n sA Mhu gpii.klALi ,ilrlli3 vl\ 60 d( hgp li
Gi6ng S6 T i n s6 kl6u g e n (s6 m l u ) Ip'n m l u
(n) AA_ ^ _ ^ G Yorkshire S6 0.70(39) 0,30(17) 0 ( 0 )
gon MC4Rit Ign Yorkshire vt Mtbng c a i
T i n s6 alelle (a6 lug-ng) He
0.65(95) 0.15(17) 0,28 0.22 ns M6ng C6I 94 0 (0) 0 (0) 1 (B4) 0 (0) 1 (188) 0 0 Z-Tesl " * ^ • " ~ ' He = Ofidi hgp l u mono dgi. PIC= HSm l u g n g IhOng tin dn hinh - , / 2 = Ph6p thO' chi binh p h u o n g , " ' = mu'c y nghra P^; 0,01
I Ol c a m o n C'mig tiiiih dirt^n hoiin ihiinli vin ••n tiii n^r kinh phi cua Dc lai ma \o lll(> 112 Id"- !>'> iliinn Oiii Plidl men Khoa lio,- vii Cnig nghe {''in, gia l \ \l OSTFD) \a / ' . • l,n md so W " / , / * „ li,: \,nig iiglii(p vii I'liiil Incn nong llidii
T A I I 11 I I M A M K H A O
Cicrbcn . Ketienbcrger d . 11 1 JA, I rp A J . liearl llilty . Keiienncrger u . i m s t r . i j / \ ,
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ticTlvib F. Jansai \ . V.TII J q i AJ. ll.irdcrs I . McinM^scn 111.
Rciicnlvryci ( i ( I W 8 ) Hie adipocyte Tally acid binding prolein liKu-.th.irjLicri/nlion and ;LvMX-ialion witti inlranuLscuLir l.il conteni in pigN. \l,iiiii>iGenome9' \f\22-]t\2(t Gerbens F. \ a n Frp A J , Harders F l . , V c r b u r g I-J.
Meuwis.scnTH, V c e r k a m p j i l , le Pas M l (l'>0'>) [urccl o!
genetic \,iii.inLs ol the heart laiiv acid-bindmg prolein gene on intramuscular Tat and pcrlormancc trails in pigs. J Aiiim Scill. K4fr-852
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P O L Y M O R P H I S M Ol- C A N D I D A T E ( i l M S \ , S S ( ) ( 1 \ 11 |) \ M | M M I A I Q U A L I I V B R E E D S O F Y O R K S H I R E A N D M O N ( ; ( \ l P U . S
Nf;uyen T h i T h u ' , \ u T I O H K LUOII-;'. Trim \ i i a n llo.iii', Ngincn Yaii <'unn};''"
'instiliile of Bioleehnology. Viclnam tcadenn at Science and Teclinohni\- -College of Fdiu olioii. Thai Nguyen I 'nnn MI\
National Inslilule of Animal lliishandrs. \ linisln of. \griciilliirc and Rural l)c\ clofiiieiil
SUMMAR^
Ihe ohjecli\es of this study wcic lo .iii.iKsc gciiclic polynioiphisms »! c.indid.ilc ycncs ,issi>i.i.ilcd wilh meat qualily in indigenous piy hived ol MOHL; ( ai and evolic pii; breed ol Yoikshite, loi this purpose, Ihe polyinorpliisms of l/nnl gene, heart faiiy .nul-hnnlin.' piuiom gene (// / //;/') iind mchmocorlin leceplor 1 gene {MC4R) w i c gcnolypod m 150 piys h> W \i ;iiid l'( IJ-KI I P MICIIUHIS For lpml gL-nc. Ihc polymorphic silcs weic found both in Voikshnc ;ind Motig ( .ii Ihe lici|uein;ics of lavouiuhlc iillele C in Yoikshire and Moiiy (.11 v\ci-c 0.71 and 0')0s ivs[KCIncly The e\|iet.ied licicio/VLiosiiics \\cic much different bclwecn
>oikshiic .md Mong l\ii and were 041 and 001. iespccii\cK (p 0,01) Kegaidme //-/.IB/* and XtC4K genes, the pol>uu>ipliisiii w.is found in Yoikshire ;md llie Ircquencics ol fa\outiihle allele <i .il H-IABP and i al \fC4R wcic 11 1' .md il 85 The cxpecied helcio/yyosiUes al llwsc IIILI \VCIC 0 23 and 0.26. icspeclivcly. In pig bleed of Mong Cai, ihcrc weie no poiymoiphism and only ihc homo/yyolc -IA and (JG vvcic found at II- F.iBP and \IC4R The compaiision of ihe polymoiphisnis ol candidate genes associalcd wilh meat qualily between indigenous biced of Mong Cai and e\olic hiced of Yoiksliire rcxealcd ihat the expected heterozygosity (He) and polymorph ism infoinialion Liinicncl (PIC) of candid.ilc genes Lpml, H-FABP and
\fC4R were higher in the e\otic pig bleed than in ihe indigenous breed ( p ' 0,01) The rcsulis reported here olTei an interesting description of llic genetic polynioiphisni of the candidate genes associated with meal qualily in indigenous and exotic pig breeds Fuilhei siudy llie lelalionship ofgenetic variaUons of candidate genes with meal qualily liails should he laken into consi derm ion to cvakuile potenlial use ol genetic markeis in marker-a.ssisied selection
Keymird.s: H-F-iBP. ipinl. \IC4R meat quality. Mong Cai pig. Yorkshire
' Author for correspondence Tel. +84-4-38362430, Fax' +84-4-8363144; E-mail "Cfifwe ihl(a)vahoo com 253
