T?p chi Khoa hoc vd Cdng ngh$ 52 (2) (2014) 179-186
DANH GIA HOAT TINH DOI KHANG VI KHUAN CUA PHlTC HE NANOCHITOSAN - TINH D A U NGHE VA NANO BAC
Nguyen Thi Kim Ciic'' *, Tran Thi Kim D u n g \ Nguyin Mai A n h \ Nguyen Thi Ngoan^, Pham ViSt Cuimg^
'Vien Hoa sinh biin, Vien HLKHCNVN. 18 Hoang Qudc Viet, Cau Giay, Hd Ndi
^Vien Hda hgc, Vien HLKHCNVN. 18 Hoang Quoc Viet. Cau Giay, Hd Ndi
Email: kcnguventhi{p),2mailcom Dgn Toa soan: 11/9/2013; Chap nhan dang: 6/3/2014
T 6 M TAT
Tinh dau nghg dugc bigt la mpt ehdt eo kha nang chong oxy hoa va co tinh d6i khang vi sinh vat tot, nhat la tie che sinh trudng eiia cac vi sinh vat co kha nang gay hong qua. Tinh dau nghe da dugc sir dung tir lau tiong y hoe e6 truyen cua cac nude chau A dg chiia mgt s6 benh.
Chitosan la mpt loai polymer carbohydrate tu nhign, eo the duge tao ra bang each deacetyl hoa chitin, voi nhi^u tinh nang nhu tuong thich sinh hpc, phan hiiy sinh hoe, bam dinh mang, doi khang vi sinh vat va khong doe hai nen duoc diing lam nguyen lieu eho nhung nghign curu ung dung tiong dugc sinh hoc va thuc pham chiic nang. Chitosan duge xir li thanh cac hat c6 kieh thuoc nano va dugc su dung nhu he dan truygn thuoc va chuyen gen. Nham tao ra mpt san phim tu nhien de bao quan qua tuoi sau thu hoach, thay thg eho cac loai hoa chat vin thutmg dugc dung, boat tinh doi khang vi khuan Gram am va Gram duong eua nanochitosan (NCS), NCS va tinh diu nghe (TDN) va NCS tinh diu nghe va hat nano bac (NB) da dugc xac dinh. Ket qua nhan dugc cho thay NCS, NCS-TDN va NCS-TDN-NB khong nhiing co hoat tinh tie ehg sinh trudng eiia hai ehimg vi khuan Bacillus cereus, va Listonella damsela ma eon eo kha nang diet khuan. Viec ket hgp NCS voi TDN va NB lam tang dang ke kha nang khang khuan cua hon hgp.
Tic khoa: Bacillus cereus, Listonella damsela, nano chitosan, nano bac, tinh diu nghe.
1. M d B A U
Trong cac loai polyme, chitosan duge nghien eiiu manh nhat boi tinh tuong thieh sinh hpc, phan huy sinh hoe, khong dpc va dl san xuat vai nguon nguygn lieu doi dao. Chitosan dugc dac biet ehii y boi kha nang on dinh hat nano kim loai. Chitosan eiing eo nhiing dac tinh sinh hpc thii vi khac nhu doi khang vi sinh v|t, cam iing su khang benh cua thuc vat, kieh thieh hoac tie ehg cac loai te bao khae nhau 6 nguoi. Vi vay, chitosan dugc sir dung rpng rai tiong nhieu linh vuc nhu y hpc, thue phim va cong nghe hoa hpc, dugc hpc, dinh duong va nong nghigp. Kha nang
Nguyin Tiii Kim Cue, Tdn Thi Kim Dung, N. Mai Anh, N. T. Ngoan, Pham Vi§t Cieom
doi khang cua chitosan da dugc chung minh la do cac dac tinh cationic cua no gay hong mang t^
bao vi sinh vat [1].
Hat nano chitosan co dien tich tiep xue va dien tich dirong Ion hon chitosan thong thuong nen c6 hieu qua khang khuan cao hon nhieu lan so vai chitosan. Chitosan cung dugc sir dung ket hgp vgi cac loai hat nano khac de tang cuong hoat tinh sinh hgc cua che phim, do la su k6t hop cac dac diem c6 loi ciia cac thanh phan trong che pham [2].
Tinh dau nghe la san pham dugc chiet xuat tir than r l cua cay nghe va da dugc chung minh c6 hoat tinh doi khang vi sinh vat, chong oxy hoa, ch6ng khoi u va hieu qua kich thich len h8 mien dich [3]. Tinh dau nghe da dugc nghien ciiu su dung de bao quan qua tuoi sau thu hoach v^
da nhan dugc nhimg kgt qua kha quan [4].
Tac dung diet khuin ciia mu6i Ag da dugc chii y tit xa xua. Dugc hik, ion bac va cac hop chat chiia bac rat doc doi voi vi sinh vat. Hien nay, Ag dugc sii dung d6 kiSm so4t sinh truong cua vi khuan trong nhieu ITnh vuc cua y hpc nhu iiha khoa, 6ng thong tiiu va cac vlt bong. Viec ket hgp nano bac voi cac dai phan tu dung lam chat hoc be mat co hieu qua d6i khang vi sinh v5t rat tot [5]. Mac dii tinh diu nghe co hoat tinh d6i khang vi sinh vat t6t, nhung viec sir dung o nong dp cao van co tac dong khong tit ddi voi vat chii boi tinh diu co tinh nong, gSy kich thich da. Nham khai thac nhChig tinh chat smh hgc co Igi cua chitosan va tinh diu nghe, dac biSt cai thien hoat tinh doi khang vi sinh vSt ciing nhu han chi tac dung khong mong mu6n cua tinh djii nghe, tinh dau nghe da dugc lien ket vgi nanochitosan bSng phuang phap phan tan pha va bay hoi dung mot. Ngoai ra, NCS-TDN ciing dugc kit hgp vol nano bac va danh gia tac duns khane
khuan ciia hgp chat nay. ' 2. VAT LIEU VA PHlTONG P H A P
2.1. Vat lieu
.ilnJlf d U r - t " " " r . ° T - ^ " ' ^'T'"" ''"""'''•• ™ °"^ M ^""""^ e r a , , tir hp suutap
? r S S u « ' c ? v ? S n ' ™ ' ^ " ^ ' ' ' ^ ' " ' ' - ' ^ - ^ ' ^ " « ° " ' " ^ « ^ " - S "
Cac loa, nanochitosan (NCS), nano bac (NB), NCS bgc tinh dSu nghe duoc cung cto boi 1 S ™ d t ? ! - ™ ' f " f "'*-'"'^' ^ ' ^ " "™ "'=• Ti 1? nano bac va NCs'-TDN la
2.2. Phinmg phap
2.2.1. Chuan bi nanochitosan vd nanochitosan-tinh ddu nghe
acetic acid va 0 , 5 % f d S ? h TPP B l T n T ' H ^1,'''.''™« " ' ' ' ' ° ' ^ > * " " ' ' " """^ ' ''"
1500 v/ph trong 1 gioTfo "C B Y L . ti' nv^l ^ T . " ' ° ?^ ""•* ''*^" ^^ •*"^>' "«" * khuSy trong 15 phlt sludo Hln^ ll ^ ™* ' " ^ "^^ =*" * ' « ' ™ "-mgdich vatilptuc moi e'thanof hoaS'toanX 2 r S c ^ T l l T o ^ l 1"" ' T *" ' '°°^ " ^ ^ ^^^ ""'^""^
tich bl mat bing Zetasizer 1.2 ( M o . Z t H t v A ! ^ ' ^ ' ' " * """^ ' ' ' ™ ™ ' ° " ' °
180
Danh gta hoat tinh doi khang vi khuSn cua phCrc h0 nanochitosan - tinh dSu nph$ va nano bac
2.2.2. Xac dinh ndng do uc che toi thieu
_ Nong dp uc ehg t6i thi6u (MICs) cua NCS, NCS-TDN va NCS-TDN-NB dugc xae dinh bang phuang phap xem dp due eua dich nuoi ciy. Bo sung vao ong diu tien co chua 200 pi moi tiirong MPB 200 (il NCS hoac NCS voi TDN va NB. Sau khi trpn dgu, 200 pi hon hgp duge ehuyen sang ong thii 2 da co 200 pi moi tiirong long, tipn dgu. Tiep tue chuygn 200 pi hdn hgp tir ong thir 2 sang ong thii 3...Nhu vay, moi ong se co mpt nita nong dp eua 6ng truoc do. Sau khi pha loang, cac 6ng dugc bo sung vi khuin den n6ng dp cuoi ciing 10^ CFU/ml. Sau khi tipn deu, eae ong duge nuoi 6 37 °C, 24 gio. Doi ehimg chi co moi truong nuoi ciy va vi smh vat.
MIC dugc dpc sau 24 gia nuoi eay tuang duong vai n6ng do tiong 6ng khong nhin thiy su phat tiien ciia vi khuin. N6ng do thip nhit cua NCS, NCS-TDN va NCS-TDN-NB lie ehg sinh truong eua vi khuan dugc tinh la MIC.
2.2.3. Xac dinh ndng do diet khudn tdi thieu (MBC)
MBC dugc xae dinh bang each eay tiai 100 |il dich nuoi eay tir cac 6ng thi nghiem MIC ma khong nhin thay su phat trign cua vi khuin len mb\ truong MPA (1 % peptone, 0,05 % yeast extiact, 0,05 % NaCl, 1,6 % agar), nuoi o dieu kien thich hop. MBC la n6ng do o 6ng khong co sinh truong ciia vi khuan (khong co khuan lac tign moi trudng dac). Cac thi nghiem duge nhic lai 3 lan cho moi chung vi khuin.
3. KET QUA VA THAO LUAN 3.1. Tinh chat cua nanochitosan va nanochitosan-tinh diu nghe
Nanochitosan va nanoehitosan-tinh dau nghe dugc chuin bi nhu mo ta, dung djch thu duge CO mau tiang siia, khong xuat hien hien tugng phan lap, tuong doi bgn dual dieu kien thuong.
Ca hai loai hat nano dugc chup FESEM (hinh 1) va dien tich be mat dugc do bang may Zetasizer. Ket qpa cho thay NCS va NCS-TDN eo zeta potential tuong duong nhau (39,0 + 5,7 va 40,5 ± 4,61 mV, tuong ung), trong khi do NCS-TDN-NB co zeta potential nho hem khong dang ke (34,7 ± 6,47 mV). Thg Zeta la mpt dae tnmg ve dien ciia cac hat keo tao su ben vung ciia cae he keo, chong lai sir keo tu, the zeta eang Ion thi he keo cang bgn, cang kho ket tiia. Cac loai hat nano chitosan thu dugc deu mang dien tich duong, va kha ldn (> 34), vi vay cac hat nanochitosan ben viing tiong dung dich va khong tao tiia sau thoi gian bao quan [6].
Hinh 1. Anh FESEM ciia cac hat nanochitosan (A) va nanochitosan -tinh dau nghf (B).
Nguyen Thi Kim Cue, Tr§n Thi Kim Dung, N. Mai Anh, N. T. Ngoan, Ph^m Vi^t Cu-drng
3.2. X&c dinh hoat tinh doi khang
Hai chung vi khuin nghign cim dugc boat hoa qua dem, sau do, bo sung vao moi tnrdng MPB da CO sin NCS, NCS-TDN, NB, NCS-TDN-NB dgn ndng dp cu6i cimg khoang lO'CFU/ml. Bang 1 so sanh boat tinh khang khuin ciia nanochitosan va nano bac d£ing tir do va dang lien kgt vai tinh dau nghe.
Bang 1. MICs va MBCs ciia NCS, NCS-TDN, NB, NCS-TDN-NB d6i vdi L.damsela vk B.cereus (ng/ml).
v i khuan
L.damsela B.cereus
NCS MIC
125 125
MBC 250 250
NCS-TDN' MIC
125 125
MBC 250 250
NCS-TDN' MIC
63 63
MBC 125 125
NB"
MIC 10
8 MBC
15 10
NCS-TDN-NB MIC
20 20
MBC 40 40 Chu thich. NCS-TDN : TDN dugc boc trong hat NCS; NCS-TDN^- TDN dugc b6 sung true tiep vio NCS trudc khi lam thi nghiem; *; nong do nano bac dugc tinh bjng ppm.
Hinh 2. Hoat tinh khang Ldamselia cua cha phim NCS-TDN-NB.
1. Chung L. damsella doi chung
2. Chiing L damselia sau Idii nuoi ciy trong moi truong co NCS-TDN-NB 20 ng/ml 3. Chiing L damsella sau khi nuoi ciy trong moi truong co NCS-TDN-NB 40 ug/ml.
Bang I cho thay cac chimg vi khuin bi ure chi sinh truong hoan toan o n6ng do 0,025 % cac chat nghien cilu bo sung vao moi tradng nuoi ciy. N6ng do cac chit b l sung vao moi tnrdng giam xuong V2, sinh truong ciia hai chimg Listonella damsela va Bacillus cereus cung bl lie che tm hon 99 % so voi do; chiing (hinh I). Bang I va hinh 1 cung cho thiy, TDN b6 sung trac tilp vao mot truong nuoi cay cung voi NCS co hoat tinh khang khuin manh hon so v « TDN duoc bpc tron| hat NCS; 0 niing dp 125 ng/ml, sinh truong ciia ca hai chung vi k h u k nghien citu da bl uc che hoan toan. Dieu nay co the giai thich do co chi hoat dong cua tinh diu thvrc vat noi Chung va TDN noi neng tren te bao vi sinh vat [6]. Vi vay, khi TDN dugc boc trong NCS, kha nang tiep xue cua tmh dau voi mang te bao vi khuin bi han chl, din din lam giam hoat tinh khang khuan cua tmh dau Nhung co the day cung lit mpt Igi thi boi TDN se dugc giai phong ra 7 « ^ b ; •'• I- - r i ^ J " f ™ "^^ ™ • * " " ? ™ ™° " o - g "hihig lo»i q"^ viing can nMSt dot nhu vai, nhan; gay krch thich vo qua...,va co thi keo dai tac dung bao quan
Nguyen Thi Kim Cue *
NB ed hoat tinh khang khuin tot han NCS va TDN. 6 nong dp 15 ppm, NB uc ehg hoan toan sinh trudng eua chiing L. damsella, va chimg B. cereus man cam hon, bi tieu diet d ndng dp NB bd sung vao mdi trudng la 10 ppm.
Khi gin nano bac vao hgt NCS-TDN, ho^t tinh khang khuin eiia hgp chit tang manh. Chi vdi 20 pg/ml, sinh trudng ciia hai chimg vi khuin nghien euu da khong phat hien dugc tiong mdi trudng long bing mit thudng (bang 1, hinh 2). Day cung la kgt qua dg higu bdi bac dugc biet la mpt chat khang khuin manh. Cac hat nano bae lign ket vdi peptidoglican d thanh te bao vi khuin, gay lie ehg kha nang van chuygn oxy vao bgn trong te bao, din dgn lam te liet vi khuin.
Sau dd, cae hat nano bac se tham nhap vao bgn tiong t8 bao, tuong tac vdi eae enzym tham gia vao qua trinh ho hap, va lie ehg qua trinh hd hip cua vi khuin [5, 10]. Kgt qua tren bang 1 eiing cho tiiiy Axcgc MICs cua NCS, NCS-TDN' cho ca 2 ehung Listonella damsela va Bacillus cereus la 250 pg/ml, eua NCS-TDN^ la 125 pg/ml, va cua NCS-TDN-NB la 40 pg/ml.
Cd kha nhigu nghien ciiu vg boat tmh khang vi khuin eiia TDN tir cac viing khae nhau, ehu yeu la tir cac nude ehau A nhu An Dp, Trung Qudc, Myanma, Thai Lan, Viet Nam. Hoat tinh nay phu thuoc vao thanh phan hoa hpc eua TDN nhan dugc bang cae phuong phap khac nhau hoac tu cac loai nghg khae nhau [U, 12, 13, 14, 15]. Chit chiu tiach nhiem chinh tiong boat tinh ddi khang vi sinh vat duge eho la ar-turmeron, nhung nhigu nghign euu eho thay hoat tinh nay la kgt qua tae dong hiep ddng eua eae thanh phan tiong tinh dau [2, 9, 12]. Tinh diu nghe trong nghign eiiu nay ed thanh phan ar-turmeron chigm tdi hon 30 %, ngoai ra edn cd mot sd monoterpenes, sesquiterpenes khae [4]. Ndng dp ire ehg toi thigu ciia TDN len vi khuan khong chi phu thuoc vao thanh phan tinh diu, ma cdn phu thudc vao ban chat ciia eae ehimg vi khuan nghien ciiu. Ndu chi su dung TDN, ndng dp ire ehg tdi thigu cho B. cereus la 1 mg/ml va eho L. damsela la 3 mg/ml [15]. Cd the thay khi TDN ket hgp vdi NCS, hai chat nay cd kha nang hd tig cho nhau, lam tang hoat tinh diet khuan eua hat (bang 1, hinh 1).
TDN la mpt chat rit mSn cam, cd thg dg dang bi phan buy dudi tac dong eua oxy, anh sang va nhiet do trung binh. Ngoai ra, ehiing khdng tan tiong nudc va d ndng dp cao gay kich thieh vi vay cin phai cd mdt cdng thiic thieh hgp cho TDN de khdng nhiing bao ve cac hgp chat ehite nang sinh hoc ma cdn giam hieu iing kieh thieh eua n6. Gan day, mpt sd nghign ciiu ve hoat tinh sinh hpc eua hat NCS-tinh dau dugc eong bo. Chen va es. (2009) sir dung phan iing Sehiff base dg gin hai thanh phan ciia tinh dau Carvacrol va Eugenol vao hat nanochitosan. Thir nghiem khang khuin dugc tien hanh vdi hai ehung vi khuan E. coli va S. aureus va thay rang, hoat tinh khang khuin eiia cae hat NCS ghep vdi thanh phan cua tinh dau bang, hoac tdt hon hat NCS khdng bi thay ddi [3]. Hat nanochitosan mang earvaerol eo hoat tinh khang lai Staphylococcus aureus. Bacillus cereus va Escherichia coli vdi MIC la 0,257 mg/mL [16]. Tinh dau eiia Lippia sidoides dugc bpc tiong hdn hgp hat nano angieo/ehitosan va la mot cdng eu hiia hen de kigm soat sdt xuit huygt [17]. Mae du tiong nghien curu nay, tinh dau nghe dugc sir dimg la dang tho, nhung boat tinh khang khuin ciia NCS-TDN doi vdi dai dien vi khuan gram(-) va Gram (+) tuang duong so vdi ket qua cua Keawehaoon & Yoksan.
Hoat tinh ddi khang vi khuan E.coli va Staphylococcus aureus eiia nano bac duge Kim va es (2007) congbd vdi MIC la> 3,3 nMeho£.co/i va> 33 tiM cho Staphylococcus aureus [18].
Hieu qua khang khuin ciia chitosan dugc bao cao do m|t dp cae nhdm amino day dac lam eho polymer co dien tich duong va tao thanh cac phiic hgp polyelecttolyte vdi peptidoglycans cua thanh te bao vi khuin. Su tuong tae nay pha hdng mang tg bao va dan dgn lie che sinh tiirdng cua vi khuan [19].
Nguydn Thi Kim Cue, Tran Thi Kim Dung, N. Mai Anh, N. T. Ngoan, Pham Viet Cu^ng
Ket qua nhan dugc cho thay NCS, TDN va NB cimg ket hgp lam tang kha nang khang khuan len nhieu lan, nong do 40 |ig/inl hgp chat nay da ire che hoan toan sinh trutmg ciia hai chung vi khuan nghien cim.
4. KET LDAN
Tinh rnan cam ciia hai chiing vi khuan Gram (-) L. damsella va Gram (+) B. cereus toi cac loai chl phim NCS, NCS-TDN, va NCS-TDN-NB tuong duong nhau, chung B. cereus mSn cam v6i NB hon so vgi chung L. damsella. MICs cho cac chimg vi khuin nghien ciiu la 125 pg/ml (NCS-TDN), va 20 ng/ml (NCS-TDN-NB); MBC ciia NCS-TDN cho ca 2 chimg vi khuin Gram (-) va Gram (+) nghien ciiu la 250 ug/ml va MBC cua NCS-TDN-NB la 40 pg/ml. Tac dung diet khuan cua NCS-TDN va NCS-TDN-NB cho thiy tiem nang iing dung ciia chimg trong y hpc va nong nghiep nhu cac chit khii hiing hoac bao ve thuc phim.
TAI LIEU THAM KHAO
1. Qi Lifeng, Zirong Xu, Xia Jiang, Caihong Hu and Xiangfei Zou - Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate Res 339 (16) (2004i 2693-2700. > > «. / 2. Chen Fei, Zhilong Shi, Neoh K. G., Kang E. T. - Antioxidant and Antibactenal Activities
of Eugenol and Carvacrol-Grafted Chitosan Nanoparticles, Biotechnol Bioene 104 (li (2009) 30-39. DOI 10.1002/bit.22363. s- I ; 3. Zeng YongChi, Feng Qiu, Kyoko Takahashi, JianMu Liang, GeXia Qu and XinSheng Yao - New Sesquiterpenes and Calebin Derivatives irom Curcuma longa, Chem Pharm
Bull. 55 (6) (2007) 940-943. * 4. Nguyin Thi Kim Ciic, va cong su - Bao cao ting kit B I tai Dpc lap cip Nha nudc"
Nghien euu cong nghe san xuat va ling dung chl phim sinh hoc tir thuc vat co chiia taclf' 'mZi'lOu" "'"''''"''P™'" ™ ' " ™ ' ' ™ •'0"g bao quan qua tuoi sau thu 5. Kim Jun Sung, Eunye Kuk, Kyeong Nam Yu, Jong-Ho Kim, Sung Jin Park, Hu Jang Lee, K m V o l ? ! ; T r? r " * T"*' ^ ™ 8 " ° ^ " * ' Cheol-Yong Hwang. Y o n g - L o . ; K m Yoon-Sik Lee, Dae Hong Jeong, Myung-Hamg Cho - Amimicrobial effects of silver nanoparticles, Nanomedicine: Nanotechnology, Biology, and Medicine 3 (2007) 95-101
"• ™ r c i l " ' ° ' ^ ' ' p ' ^ ' '^""'^" ^ ' ' ' " ' '"^ •"•'^'"" Chao - Preparation and properties of nanochitosan. Polymer-plastics Technology and Engineerine 48 (2009i 1 5 n n i 10.1080/03602550903159069. S neenng 4S (2009) 1-5. DOI.
7. Paula HCB, Sombra FM, Abreu POMS and de Paula RCM ronim r- • j -j, f K n t 2 3 ° 5 l 2 3 " 6 6 " " ' " ° " ^ ^ " " « - ™ « " ^ ™ - ' ^ " ^ ^ ^ ^ ^ ^ ^ ^ ^
' rittX''p'.an?iL'?.' '^:^.^^r-f^ - --^-^ °^
efficacy, titer. J. Drug. Delivery 3 ( 2 0 ^ ) aSllsSS ' " " " " .nnnunorestoratory
' r:x;e^T?o:dMi:::s;r^^?oS5ss^^"''°'^"''^'-''«^^°-
Nguyin Thi Kim Cue *
10. Singh M., Shinjini Smgh, Prasad S., Gambhir I. S. - Nanotechnology in medicine and antibacterial effect of silver nanoparticles digest, J. Nanomaterials and Biostmctiires 3 (3) (2008)115-122.
11. Phan Minh Giang, Van Ngoe Huong, Phan Tong Son - Antimicrobial activity of sesquiterpene constituents from some Curcuma species of Vietnam, Vietnamese J.
Chemisti^ 38 (1) (2000)91-94,
12. Norajit K., Natta Laohakimjit and Orapto Kerdchoeehuen - Antibacterial Effect of Five Zingiberaceae Essential Oils, Molecules 12 (2007) 2047-2060.
13. Prabuseeruvasan S., Manickkam Jayakumar and Savarimuthu Ignacimuthu - In vitro antibacterial activity of some plant essential oils, BMC Complementary and Altemative Medicine 6 (2006) 39. DOI:10.1186/1472-6882-6-39.
14. Ph?m Viet Cudng, Tran Thi Kim Dung, Nguyen Thi Kim Cue - Anh hudng eiia thanh phan dieh chigt titih diu nghe len kha nang ire ehe sinh trudng in vitro eiia vi khuan. Tap ehi Cong nghe smh hoe 8 (3A) (2010) 757-763.
15. Nguyen Thi Kim Cue, Tran Thi Kim Dung, Pham Viet Cuong - Antimicrobial effect of turmeric oil (Curcuma longa L.), Tap chi Khoa hpc va Cdng nghe 48 (5) (2010) 37-45.
16. Keawehaoon Lalita, Rangrong Yoksan - Preparation, characterization and in vitro release study of carvaerol-loaded chitosan nanoparticles. Colloids and Surfaces B: Biomterfaees 84(1) (2011)163-171.
17. Haroldo C. B. Paula, Fernanda M. Sombra, Flavia O. M. S. Abreua and Regina C. M. de Paula Lippia sidoides Essential Oil Encapsulation by Angico Gum/Chitosan Nanoparticles, J. Braz. Chem. Soc. 21 (12) (2010) 2359-2366.
18. Kim J. S., Kyeong Nam Yu, Jong-Ho Kim, Sung Jin Park, Hu Jang Lee, So Hyun Kim, Young Kyung Park, Yong Ho Park, Cheol-Yong Hwang, Yong-Kwon Kim, Yoon-Sik Lee, Dae Hong Jeong, Myung-Hauig Cho - Antunierobial effects of silver nanoparticles, Nanomedicine: Nanotechnology, Biology, and Medicine 3 (2007) 95-101.
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ABSTRACT
EVALUATION OF ANTIBACTERIAL ACTIVITY OF MIXTURE CONTAINING TURMERIC OIL AGAINST BACTERIA
Nguyen Thi Kim Cue'' *, Tran Thi Kim Dzung', Nguyen Mai Anh', Nguyen Thi Ngoan^
Pham Viet Cuong^
'institute of Marine Biochemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi
^Institute of Chemistry, VAST, 18 Hoang Quoc Viet. Cau Giay. Ha Noi
'Email: kcnguventhi&.2mail.com
Nguyin Thi Kim Cue, Tran Thi Kim Dung, N. Mai Anh, N. T. Ngoan. Pham Viet Cu^ng
Turmeric oil has been known as possessing antioxidant and antimieroorganism activities, especially growth inhibition of firesh fruit spoiling microorganisms. Turmeric oil was used in traditional medicine for decades in Asian countnes for disease tieatment. Chitosan is a natural carbohydrate polymer, can be produced by deaeetylation of ehitin, with various features as biological compatible, biological degradation, adherent membrane, antimieroorganism and harmless, so is used for application investigation in biopharmaeology and fimction food.
Chitosan were processed into nanoparticles and is used as a vehicle for drag delivery and gene tiansformation. For making natural product in posthaverst fruit preservation as an altemative for commonly used chemicals, antibacterial activity of nanochitosan (NCS), NCS with turmeric oil (TDN) and mixture of NCS, TDN and siver nanoparticles was determined. Reeieved results showed that NCS, NCS-TDN and NCS-TDN-NB have not only growth mhibition activity against Bacillus cereus, and Listonella damsela, but also bactericidal activity. Combination of NCS, TDN and NB significantly enhances their antibactenal activity.
Keywords: Bacillus cereus, Listonella damsela, nano chitosan, nanosilver, turmeric oil.
