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῎ ῌ
1) H. Nagasawa, H. Kataoka, A. Isogai, S. Tamura, A. Suzuki, H.
Ishizaki, A. Mizoguchi, Y. Fujiwara, and A.Suzuki:Science, 226, 1344῍1345 (1984).
2) H. Nagasawa, H. Kataoka, Y. Hori, A. Isogai, S. Tamura, A.
Suzuki, F. Guo, X. Zhong, A. Mizoguchi, M. Fujishita, S.Y.
Takahashi, , E. Ohnishi, and A. Suzuki:Gen. Comp. Endocri- nol.,53, 143῍152 (1984).
3) H. Nagasawa, H. Kataoka, A. Isogai, S. Tamura, A. Suzuki, A.
Mizoguchi, Y. Fujiwara, A. Suzuki, S.Y. Takahashi, and H.
Ishizaki:Proc. Natl. Acad. Sci. U.S.A.,83, 5840῍5843 (1986).
4) H. Kataoka, H. Nagasawa, A. Isogai, S. Tamura, A. Mi- zoguchi, Y. Fujiwra, C. Suzuki, H. Ishizaki, and A. Suzuki:
Agric. Biol. Chem.,51, 1067῍1076 (1987).
5) A. Kawakami, M. Iwami, H. Nagasawa, A. Suzuki, and A.
Suzuki:Proc. Natl. Acad. Sci. U.S.A.,86, 6843῍6847 (1989).
6) K. Maruyama, K. Nagata, M. Tanaka, H. Nagasawa, A. Isogai, H. Ishizaki, and A. Suzuki:J. Protein Chem.,11, 1῍12 (1992).
7) K. Nagata, K. Maruyama, H. Nagasawa, I. Urushibata, A.
Isogai, H. Ishizaki, and A. Suzuki: Peptides, 13, 653῍662 (1992).
8) K. Nagata, K. Maruyama, K. Kojima, M. Yamamoto, M.
Tanaka, H. Kataoka, H. Nagasawa, A. Isogai, H. Ishizaki, and A. Suzuki: Biochem. Biophys. Res. Commun., 266, 575῍578
(1999).
9) Q. X. Hua, S. E. Shoelson, M. Kochoyan, and M. A. Weiss:
Nature,354, 238῍241 (1991).
10) K. Nagata, H. Hatanaka, D. Kohda, H. Kataoka, H. Nagasawa, A. Isogai, H. Ishizaki, A. Suzuki, and F. Inagaki:J. Mol. Biol., 253, 749῍758 (1995).
11) C. Eigenbrot, M. Randal, C. Quan, J. Burnier, L. O’Connell, E.
Rinderknecht, and A.A. Kossiako#:J. Mol. Biol.,221, 15῍21 (1991).
12) H. Jhoti, A. N. McLeod, T. L. Blundell, H. Ishizaki, H. Na- gasawa, and A. Suzuki:FEBS Lett.,219, 419῍425 (1987).
13) K. Nagata, H. Hatanaka, D. Kohda, H. Kataoka, H. Nagasawa, A. Isogai, H. Ishizaki, A. Suzuki, and F. Inagaki:J. Mol. Biol., 253, 759῍770 (1995).
14) J. Murray-Rust, A. N. McLeod, T. L. Blundell, and S. P. Wood:
BioEssays14, 325῍331 (1992).
15) L. Scha¤#er:Eur. J. Biochem.,221, 1127῍1132 (1994).
16) H. F. Nijhout and L. W. Grunert:Proc. Natl Acad. Sci. U.S.A., 99, 15446῍15450 (2002).
17) K. D. Kimura, H. A. Tissenbaum, Y. Liu, and G. Ruvkun:
Science277, 942῍946 (1997).
18) T. Kawano, R. Nagatomo, Y. Kimura, K. Gengyo-Ando, and S. Mitani:Biosci. Biotechnol. Biochem.,70, 3084῍3087 (2006).
19) S. Paradis, M. Ailion, A. Toker, J.H. Thomas, and G. Ruvkun:
Genes Dev.,13, 1438῍1452 (1999).
20) M. Tomioka, T. Adachi, H. Suzuki, H. Kunitomo, W. R.
Schafer, and Y. Iino:Neuron,51, 613῍625 (2006).
21) K. Nagata, D. Kohda, H. Hatanaka, S. Ichikawa, S. Matsuda, T. Yamamoto, A. Suzuki, and F. Inagaki:EMBO J.,13, 3517῍ 3523 (1994).
22) H. Terasawa, D. Kohda, H. Hatanaka, K. Nagata, N. Higashi- hashi, H. Fujiwara, K. Sakano, and F. Inagaki:EMBO J.,13, 5590῍5597 (1994).
23) W. Iwasaki, K. Nagata, H. Hatanaka, T. Inui, T. Kimura, T.
Muramatsu, K. Yoshida, M. Tasumi, and F. Inagaki:EMBO J., 16, 6936῍6946 (1997).
24) K. Ogura, K. Nagata, H. Hatanaka, H. Habuchi, K. Kimata, S.
Tate, M. W. Ravera, M. Jaye, J. Schlessinger, and F. Inagaki:
J. Biomol. NMR,13, 11῍24 (1999).
25) H. Katayama, T. Ohira, K. Nagata, and H. Nagasawa:Biosci.
Biotechnol. Biochem.,65, 1832῍1839 (2001).
26) H. Katayama, K. Nagata, T. Ohira, F. Yumoto, M. Tanokura, and H. Nagasawa:J. Biol. Chem.,278, 9620῍9623 (2003).
27) H. Terasawa, D. Kohda, H. Hatanaka, S. Tsuchiya, K. Ogura, K. Nagata, S. Ishii, V. Mandiyan, A. Ullrich, J. Schlessinger, and F. Inagaki:Nature Struct. Biol.,1, 891῍897 (1994).
28) S. Tsuchiya, K. Ogura, H. Hatanaka, K. Nagata, H. Terasawa, V. Mandiyan, J. Schlessinger, S. Aimoto, H. Ohta, and F.
Inagaki:J. Biochem.,125, 1151῍1159 (1999).
29) M. Nishida, K. Nagata, Y. Hachimori, M. Horiuchi, K. Ogura, V. Mandiyan, J. Schlessinger, and F. Inagaki:EMBO J.,20, 2995῍3007 (2001).
30) K. Ogura, K. Nagata, M. Horiuchi, E. Ebisui, T. Hasuda, S.
Yuzawa, M. Nishida, H. Hatanaka, and F. Inagaki:J. Biomol.
NMR,22, 37῍46 (2002).
31) Y. Kato, M. Ito, K. Kawai, K. Nagata, and M. Tanokura:J.
Biol. Chem.,277, 10173῍10177 (2002).
32) Y. Kato, K. Nagata, M. Takahashi, L. Lian, J. J. Herrero, M.
Sudol, and M. Tanokura:J. Biol. Chem.,279, 31833῍31841 (2004).
33) Y. Kato, Y. Hino, K. Nagata, and M. Tanokura:Proteins63, 227῍234 (2006).
34) M. Tanaka, H. Kataoka,, Nagata, K., Nagasawa, H. and A.
Suzuki:Regul. Pept.,57, 311῍318 (1995).