KHOA HOC KY THUAT THO Y TAP XXVI SO 6 - 2019
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HIEU QUA Bd SUNG ASCOGEN® LEN N A N G SUAT VA D A P tfNG M I I N D|CH SAU TIEM PHONG TREN HEO NAI MANG THAI VA HEO CON THEO ME
Dodn Tran Vinh Khdnh', Nguyen Pham Huynh', Tran TrucAnh\ Klaus Hoffmann^ Do Tien Duy'
TOM TAT
Muc tieu ciia nghien cmi nay nham danh gia hieu qua cua ASCOGEN® bo sung vao thuc an dSn dap img miln dich ciia heo nai mang thai sau nem phong vacxin PRRS va CSF va heo con theo me. KSt qua nghien cuu cho thay ham lugng khang the blocking dich ta heo tren nhom heo nai dugc an thiic an c6 bo sung ASCOGEN®
{81,43±9,22} cao hon so voi nhom heo nai doi chung (80,77±9,05) vao thai diem sau tiem phong 28 ngay. (3 thai diem tru'ac tiem phong, c6 su khac biet {P<0,05) Idiang tlie thu dgng dich ta heo giiia heo con co hoac khong su dung ASCOGEt^ tit heo nai sir dung ASCOGEN® so voi heo con khong su dung ASCOGEN®
tir heo nai doi chung; va khang tlie thu dong dich ta heo cua heo con c6 su dung ASCOGEN" ttr heo nai doi chimg Cling cao han co y nghia (P<0,05) so vai heo con khong su.' dung ASCOGEN® tu heo nai doi chiing.
Ham Iugng khang the khang PRRS (S/P) sau tiem phong a heo con co sir dung ASCOGEN* tir heo nai sir dung ASCOGEN" cao han heo con khong su dung ASCOGEN® tir heo nai sii dung ASCOGEN* (P<0,01) va heo con khong sir dung ASCOGEN"" tii heo nai doi chimg (P<0,01). Su khac biet nay cung the hien tren heo con c6 su dung ASCOGEN" tir heo nai doi chiing so voi heo con khong su dung ASCOGEN® tii heo nai doi chiing (P<0,05). Co sir khac biet thong ke giiia 16 doi chung so vai 16 thi nghiem (bo sung ASCOGEN*') a kh6i lugng heo sa sinh: 1,54 kg±0,23 so voi 1,38 kg±0,26 (P<0,05) va kh6ng co su khac biet 6 khoi lugng heo cai siia 6,2 kg±0,99 so voi 5,98 kg±l ,09 (P=0,060). KSt qua thi nghiem cho thdy b6 sung ASCOGEN® vao khSu phSn cua heo CO hieu qua cai thien dap iing mien dich sau tiem phong va cai thien nang suat heo
Tif khoa: ASCOGEN*, dap irng miln dich, heo.
Affect of ASCOGEN^ supplementing in feed to immune response of gestational sows after PRRS and CSF vaccination and sucking piglets
Doan Tran Vinh Khanh, Nguyen Pham Huynh, Tran True Anh, Klaus Hoffmann, Do Tien Duy SUIVIMARY
The objective of this study was to evaluate the affect of ASCOGEN® supplementing in feed to the immune response of the gestational sows after PRRS and CSF vaccination and the sucking piglets. The studied results showed that the antibody content against hog cholera in the sows eating supplemented ASCOGEN® was (81.43 ± 9.22), higher than that of the control sows {80.77 ± 9.05) after 28 days of vaccination. In the time of before vaccination, there was a difference (P <0.05) of maternal derived ' Khoa Chan nuoi Thii y Dai hpc Nong Lam Tp. HCM
- Cong ty TNHH thuang mai tigp thi CMS, Viet Nam
^ Chemoforma Ltd., Rheinsnasse 28-32, CH-4302 Augst, Switzerland
KHOA HOC KY THUAT THU Y TAP XXVI SO 6 - 2019
antibody (MDA) in the sucking piglets feeding or un-feeding ASCOGEN® came from the sows feeding ASCOGEN® in comparison with the sucking pigleis un-feeding ASCOGEN® came from the control sows;
and MDA of the sucking piglets feeding ASCOGEN® from the control sows was significantly higher {P
<0.05) than that of the sucking piglets un-feeding ASCOGEN®. After vaccination, the antibody content against PRRS in the sucking piglets feeding ASCOGEN® was higher than that of the sucking piglets unfeeding ASCOGEN® came from the same sows feeding ASCOGEN® (P<0.01) and the sucking piglets un-feeding ASCOGEN® came from the control sows {P<0.01). This difference was also expressed in the piglets feeding ASCOGEN® in comparison with those of un-feeding ASCOGEN® came from the control sows {P <0.05) There was a significant difference on the weight of the new-born piglets came from the sows feeding ASCOGEN® and the control sows (1.54 kg±0.23 versus 1.38 kg+0.26), respectively (P <0,05). Similarly the weight of the weaning piglets came from the experimental and control sow groups was 6.2 kg±0.99 versus 5.98 kg±1.09, respectively (P=0.060). The studied results showed that ASCOGEN® supplementing in feed was effective in improving the immune response of the gestational sows after vaccination and the sucking piglets as well as improving the piglet growth performance.
Keywords: ASCOGEN®, immune response, pig.
I. DAT VAN DE
Nhieu giai phap thay the khang sinh trong phong tri benh dugc lua chgn trong tho'i gian qua tren heo, g6m co vacxin phong benh, probiotics, prebioties, dau chiet xuat tir thuc vat, peptide khang khuan, khang the thu dgng, men tieu hoa, acid huu ca, hay thuc khuan the,...vcti cac ca che tac dong true tiep hay gian tiep khac nhau (Thacker, 2013). Han niia, chiet xuat nam men ca thanh te bao nam men (heta-glucans) hay nhan te bao nam men (nucleotides) ciing dugc de cap den vai tinh nang tang cuong kha nang miln dicJi ciia thu nuoi va ciing la nguon bo sung gia tri dinh duong. Nucleotides la hgp chat sinh hgc tham gia vao qua trinh phan chia cua te bao nen no tham du vao su tang truong va dap iing mien dich ciia dgng vat (Carver va Walker, 1995;
Whitehead va ctv., 2006; Rutz, 2007). Cho du la giai phap lira chgn nao de thay thi khang sinh thi con duong thiic day tang siic khang benh bing mien djch true tiep hay gian tiep ciing la mgt lua chgn t6i im va hiia hen bo trg hieu qua cho cac giai phap phong benh kliac.
Nucleotides la thanh phan can tao ciia acid nucleic, RNA va DNA. Nucleotides va cac dan chat cua chiing co vai tro quan trpng trong qua trinh chuyen hoa sinh hgc ciia ca the (Whitehead va ctv., 2006). Nucleotides co kha nang cai thien kha nang hap tliu chat dinh duong, tham gia vao qua trinh phan chia ciia te bao nen tang cuong hoat dgng cua he thong miln dich, tiid6
giup thii de khang vai cac tac nhan gay benh t6t hon (Tibbetts, 2007; Rutz va ctv., 2007; Hess va ctv., 2012). Nhiing te bao thay the mai lien tue trong CO the deu phai sir dung nucleotides de hinh thanh nhan te bao qua qua trinh sinh tong hgp DNA va RNA. Nucleotides rat can thiet cho cac hoat dgng ciia he thong mien dich de sira chiia te bao sau khi bi ton thuang do benh tat va giiip ngan ngira benh. Tac dung cai thien tang truang va dap iing mien dich sau tiem phong ciing dugc bao cao bai mgt so tac gia (Ramadan va Atef, 1991; Ramadan va ctv., 1994). Ngoai ra, nucleotides cai thien su phat trien cua nhung mao rugt, tang chieu dai nhung mao rugt lam cho dien tich be mat cua rugt Ion hon va su hap thu chat dinh duang tot han (Bustamante va ctv, 1994; Cheng va ctv., 2011; Peng va ctv., 2013).
ASCOGEN® la mgt che pham thuang mai (CEfEMOFORMA, Thuy Sy), la t6 hgp thanh phan chiia RNA tinh chi dugc trich tir nSm men, nucleotides tinh che, tien chat ciia nucleotides, cac acid hihi ca va nam men vo boat. Cac thanh phan chinh ciia che pham se giup gia tang nang suit chan nuoi th6ng qua viec gia tang dap iing mien dich, hieu luc cua vacxin, cai thien va gia tang kha nang hap thu, tieu hoa duong chat. Tac dung ciia chi pham nay dz dugc thir nghiem chiing minh hieu qua tren gia cam va ca a nhieu nuac (Thongwittaya va ctv, 1992; Pelicia va ctv., 2009; Yu va ctv, 2002) nhung th6ng tin ket qua thii nghiem fren lieo con han che, nhat la o vai tro kich thich dap una miln
KHOA H O C KY THUAT THtT Y TAP XXVI SO 6 - 2019
dich sau tiem phong. O cac trai chan nuoi heo, viec tiem phong benh dich ta heo va hgi chimg r6i loan sinh san va ho hdp (PRRS) dugc chii trgng thuc hien thuang quy do tinh chat quan trgng v l dich t l cua chiing. Do d6, m u c tieu cua nghien ciru nay n h ^ danh gia anh huong cua ASCOGEN® d i n viec nang cao dap irag mien dich ciia vacxin (dich ta heo va PRRS) sau tiem phong tren heo nai mang thai va heo con theo m e tai mgt trai chan nuoi heo thuong pham.
II. VAT LIEU VA PHlTONG PHAP
2 . 1 . Bd tri thi n g h i e m
Thi n g h i e m d u g c thuc hien tai Trai heo nai chan nuoi t h u a n g p h a m (300 nai). T o n g so 60 heo nai m a n g thai, d o n g deu v l giong (YL) va Ilia d e (2-6), co sue khoe t6t, d u g c p h a n chia ngau nhien thanh 2 n h o m thi nghiem: n h 6 m A (doi chimg, k h o n g b o sung che p h a m trong thirc an), n h o m B (thi nghiem, b o sung che p h a m
A S C O G E N * trong thiic an theo lieu khuyen cao cua nha san xuat). H e o nai a 2 n h o m thi n g h i e m d u g c bo tri t u a n g doi d o n g deu ve tinh trang siic klioe, giong, lira de, tho'i diem sinh va d u g c c h a m soc nuoi d u o n g theo ciing quy trinh ciia trai n h u kieu chuong nhot, thiic an, each cho an, n u a c uong, k h o n g trgn kliang sinh, kliong sii d u n g cac hormone. H e o nai a 2 n h o m thi n g h i e m d u g c tiem phong theo diing quy trinh ciia trai ve thoi diem va ky thuat tiem; trong d o vacxin dich ta heo (CSF) d u g c co dinh thai diem tiem (tuan m a n g thai thii 10; Suigen H C , Virbac) va ky thuat tiem dong d e u giiia hai n h 6 m thi nghiem. H e o nai ciing d u g c tiem phong P R R S (Ingelvac M L Y P R R S ; cong ty Boehringer higelheim Vetmedica, Inc) d o n g loat moi 4 thang (3 lan/nam). O 16 heo thi nghiem, ASCOGEN® d u g c b o sung lien tue vao thiic an (phun tren be m a t cam hon h g p dang vien) bat dau t r u a c thai diem tiem phong C S F hai tuan; trong khi d o heo nai cr 16 doi chimg kh6ng bo sung che p h a m n a y (bang I A ) .
3ang 1A. Bo tri bo sung ASCOGEN® trong thi nghiem tren heo nai
Ky hieu Thirc an cvban
Doi chirng Thi nghiem
H e o con cua 15 h e o nai a m o i n h o m thi nghiem (A, B) se d u g c c h g n ngau nhien d e p h a n lam 4 n h o m thi n g h i e m (bang 1B) n h a m so sanh kha nang tang trucrng v a dap iing m i e n dich cua heo con tir nai k h o n g d u g c b o s u n g v a nai co b o sung c h e p h a m ; thai gian bo sung c h e p h a m tir khi tap an d e n cai sira (28 n g a y tuoi), v a i lieu
k h u y e n cao 1 k g / tan thii'c an. H e o con d u g c tiem p h o n g P R R S (Ingelvac M L V P R R S ; cong ty Boehringer Ingelheim Vetmedica, Inc) va C S F (Suigen H C , Virbac) liic 28 v a 35 n g a y tuoi, t u o n g ling. Q u y trinh c h a m soc, cho an uong, c h u o n g nuoi va p h o n g cac b e n h khac d e u giong nhau a hai n h o m heo.
Bang 1 8 . Bd tri bo sung ASCOGEN*^trong thi nghiem tren heo con Nhom So nai So heo con Thire an co' ban DOI chirng
Thi nghiem B1
B2
150 150 150 150
Ghi chii: A J vaA2 tir heo me khong bd sung ASCOGEN^; Bl va B2 tic heo me co bo sung ASCOGEN^
KHOA HOC KY THUAT THLJ Y TAP XXVI SO 6 - 2019
2.2. Thu mau va phan tich phong thi nghiem Huyet thanh ciia tat ca cac heo nai dugc thu thap 2 tuan truac tiem phong (ngay thai diem bo sung che pham) va 28 ngay sau tiem phong CSF de xac dinh ham lugng khang the khang CSF (Blocking titer). Thai diem tiem phong PRRS tren nai theo quy trinh dong loat moi 4 thang/lan nen thi nghiem chi theo doi dugc dap iing mien dich CSF truac va sau khi bo sung che pham.
Huyet thanh ciia 15 heo con (moi con/nai) o moi nhom Al, A2 (nai d6i chimg) va Bl, B2 (nai thi nghiem co bo sung) dugc thu thap ngau nhien de kiem tra ham lugng khang the khang PRRS va CSF truoc va sau tiem phong 28 ngay (CSF) va 35 ngay (PRRS), thoi diem tiem phong cho heo con thi nghiem vacxin PRRS liic 28 ngay tu6i va vacxin CSF liic 35 ngay tuoi. Ham lugng khang the (blocking titer) khang virus CSF dugc kiem tra theo huong dan ciia bg kit thuang mai CSFV Ab Test (IDEXX, My). Ham lugng khang thi khang PRRSV (S/P IgG) dugc xac djnh theo huong dan cua bp kit thuang mai PRRS X3 Ab test (IDEXX, My).
2.3. Xir ly thong ke
Ham lugng khang the a cac thoi diem giira 2 16 thi nghiem dugc so sanh th6ng ke bang trac nghiem pairwise t-test va su khac biet giira cac chi tieu nang suat dugc xii ly th6ng ke theo trac nghiem Tukey. Su khac biet dugc xem la c6 y nghia khi P < 0,05.
HI. KET QUA VA THAO LUAN 3.1. S a p img mien dich tren heo thi nghiem 3.1.1. Tren heo ndi
Ham lugng khang the (% Blocking IgG/
ELISA) dich ta heo tren heo truac khi tiem phong a nhom nai bo sung ASCOGEN®
(78,21±9,38) thap hern so voi nhom nai d6i chung (79,81±9,00); nhung ham lugng nay lai cao hon tren nhom nai bo simg ASCOGEN®
(81,43±9,22) so voi nhom nai doi chiing (80,77±9,05) vao thoi diera sau tiem phong 28 ngay. Tuy nhien, khong co su khac biet giiia hai nhom heo thi nghiem khi xii ly thong ke (P>0,05).
• Control
Thoi diem trtr^c ya san tiem phong
Bieu do 1. Khang the (% Blocking IgG/ELISA) dich ta heo tren heo nai
& th&i diem I (P>0,05) va & th&i diem II (P>0,05) Thai diem 1 truoc tiem phong 14 ngay; Thai diem II sau tiem phong 28 ngay
Ham lugng khang the khang dich ta heo so sanh tren heo nai giiia 16 doi chiing va 16 thi nghiem bo sung ASCOGEN® khong co su khac biet a thoi diem truac va sau tiem phong. Tuy
nhien, co sir dap iing mien dich t6t hon o thai diem sau tiem phong (81,43+9,22%) so vai truoc tiem phong (78,21±9,38%) onhom nai thi nghiem co bo sung; mac du a thoi diem truoc
KHOA HOC KY THUAT THtl Y TAP XXVI SO 6 - 2019
tiem phong, ham lugng khang the ciia nhom heo nai nay co phSn thdp hon 16 doi chiing (79,81±9,00%). Chi sl khang thi blocking titer
= 50 la cut-off cho thdy khang thi duong tinh va la ngudng bao hg chong nhiem virus CSF.
Chi so blocking titer = 50 co su tuong quan cao vdi nguong ham lugng khang thi trung hoa (NA hoac NPLA; IDEXX, My). 6 trai thi nghiem, quy trinh tiem phong CSF tren heo nai mang thai (Uian 8, 9 hoac 10) dugc duy tri 6n dinh it nhat da 3 nam truac thai gian nghien cuu, va a thoi dilm thi nghiem (tru'ac khi tiem phong), 60/60 heo nai a hai 16 thi nghiem deu co blocking titer
>50. Tiem phong vacxin CSF nhugc dgc c6 su on dinh mien dich bao hg cao tren heo nai a ca quy trinh tiem klii nai mang thai, tiem theo lua sau khi sinh va tiem dong loat 2 idn/nam (Suradhat va ctv., 2007).
3.1.2. Tren heo con
Ham lugng khang thi % Blocking IgG/
ELISA dich ta heo a heo con sii dung ASCOGEN® (ca hi heo me doi chimg va six dung ASCOGEN®) cao hon heo con khong su dung che pham; tuy nhien su khac biet chua c6 y nghTa thong ke. (3 thai diem truac tiem phong 1 tuan (28 ngay tuoi), co sir khac biet (P<0,05) khang the thu dgng djch ta heo (MDA) giua heo con c6 hoac kh6ng sii dung ASCOGEN®
tir nai sir dung ASCOGEN® (16 Bl va B2) so vai heo con kh6ng sir dung ASCOGEN® tii heo nai doi chiing (16 A2); va khang thi thu dgng dich ta heo (MDA) ciia heo con co sii dung ASCOGEN® tir nai dli chiing (16 A1) cung cao hon CO y nghia (P<0,05) so vai heo con khong sir dung ASCOGEN*' tir nai d6i chiing (16 A2) trong thi nghiem.
Heo con thf nghifm
Bieu do 2A. Ham Iwo-ng khang the (% Blocking IgG/ELISA) dich ta heo tren heo con Su khac biet thong ke a ldn xet nghiem I giua nhom Bl, B2 vs. A2 (P<0,05); vd giira nhomAl vs.
A2 (P<0,05). Th&i diem I truac tiem phong 7 ngdy, thai diem II sau tiim phong 28 ngdy Ham lugng khang the CSF heo me cao se
truyen qua sGa dau cho con ciia chiing va keo dai nhieu tuan sau klii sinh tiiy theo lugng kliang the thuc su nhan dugc tir sUa dau (Suradhat va Damrongw^atanapokin, 2003; Suradhat va ctv., 2007). Ham lugng khang the thu dgng nay rat quan trgng de bao hg heo con trong nhiing tuan dau tien va quyet dinh thai diem tiem phong
tren heo con de co hieu qua dap ung mien dich sau tiem phong vi khang the nay co the trung hoa virus vacxin song nhugc doc khi tiem cho heo con con ham lugng khang the nay cao trong CO the. Heo con tir nai bo sung ASCOGEN®
CO ham lugng khang the thu dgng (MDA) CSF cao hon so vai heo con cua heo nai doi chiing, ket qua nay cho thay co sir lien he giiia
KHOA HOC KY THUAT THU Y TAP XXVI SO 6 - 2019
khang thi huyet thanh me va huyet thanh heo con cua chiing th6ng qua siia dau (Suradhat va Damrongwatanapokin, 2003).
Ham lugng khang thi khang PRRS (S/P IgG) sau tiem phong aheo con co sir dung ASCOGEN*
tir nai sii dung ASCOGEN^ (16 Bl) cao hem heo
con kh6ng su dung ASCOGEN® tir nai su dung ASCOGEN® (16 B2) (P<0,01) va heo con khong su dung ASCOGEN^ tu nai dli chiing (16 A2) (P<0,01). Su khac biet ciing thi hien tren heo con
• CO su dung ASCOGEN^ tir nai doi chiing (16 Al) so voi heo con khong su diang ASCOGEN* tir nai doi chimg (16 A2) (P<0,05).
B2 A l Heo con thf nghidm
Bieu dd 2B. Khang the (S/P IgG/ELISA) PRRS tren heo con
Su khac biet thong ke a ldn xet nghiem IIgiira nhom A} vs A2 (P<0,05) vd giira nhom Bl vs. B2 (P<0,01) vd gitta nhom Bl vs. A2 (P< 0,01). Thai diem I truac tiem phong: thai diem II sau tiem phong 35 ngay
Trong thi nghiem nay, khang the khang PRRS tren heo me kh6ng dugc so sanh giiia 16 d6i chung va thi nghiem vi chuong trinh tiem phong theo kieu dong loat 3 lan/nam (4 thang/
lugt), va quy trinh da 6n dinh trong 2 nam gan day nen khong the b6 tri d6ng thoi de theo doi truoc va dap iing sau tiem phong cung voi tiem phong dich ta heo. Co le do tiera phong dong loat nen ham lugng khang the nhan dugc tren heo con ciia cac nai thi nghiem kha d6ng deu (P>0,05) a miic nguang duong tinh (S/
P>=0,4) tai thai diem tiem phong (28 ngay tu6i;
cai siia) ciia cac 16 Bl (S/P=0,4±0,27), B2 (S/
P=0,37±0,49), Al (S/P=0,38±0,45) va A2 (S/
P=0,41±0,28) (bilu d l 2B); va a nguang khang the nay la phii hgp cho miii tiera phong dau tien cho heo con. Sau 35 ngay tiem phong, tat ca heo
con thi nghiem deu duong tinh, va co khuynh huong cao hem tren nhom heo con c6 b6 sung ASCOGEN^ trong thiic an. Ham lugng khang the sau tiem phong PRRS (MLV vacxin) thuong dat ducmg tinh (S/P>=0,4) sau 10-14 ngay va dat cao liic 21-28 ngay (Duy va ctv., 2015) va dat dinh luc 56 - 84 ngay roi sau do giam ddn, keo dai den 6 thang (Johnson va ctv"., 2004; Nan va ctv. ,2017).
Kh6ng CO nhieu thi nghiem danh gia hieu qua ciia viec b6 sung ASCOGEN^ trong thiic an len dap iing mien dich sau tiem phong a heo, nhung nhieu nghien cuu khac tren gia cdm, ca,.. -da dugc chiing minh va bao cao (Ramadan va Atef, 1991; Thongwittaya va en, 1992;
Ramadan va ctv., 1994; PeUciava ctv . ;;)09-va
KHOA HOC KY THUAT T H C Y TAP XXVI SO 6 - 2019
cac bao cao ngi bg cua CHEMOFORMA, Thuy Sy. d thi nghiem nay, b6 sung ASCOGEN® vao khau phan cua heo nai mang thai co hieu qua cai thien dap iing mien dich sau tiem phong dich ta heo, nhat la keo dai mien dich thu dgng (MDA) va 6 heo con dugc sir dung ASCOGEN®
vao thirc an tap an ciing giup tang hieu qua dap ling mien dich sau tiem phong PRRS va dich ta heo. Nghien ciiu la buoc dau cho thay tac dung ciia ASCOGEN®, ma hong thanh phdn chinh la nucleotides c6 thi phat huy tac dung theo dac tinh sinh hgc cua n6 (Whitehead va ctv., 2006;
Tibbetts, 2007; Rutz va ctv., 2007; Hess va ctv., 2012); do do, cac nghien ciiu tiep theo vai cac chi tieu khao sat sau hon va cu the hon nhu chi so dap ling mien dich te bao (IFNs) va khang the trung hoa,,..can dugc thuc hien de chimg minh tin cay hon ve gia tri.
3.2. Nang suat sinh san va tang trong heo con thi nghifm
Co su khac biet th6ng ke giil'a 16 doi chimg (control) so voi 16 thi nghiem (b6 sung ASCOGEN®) a trgng lugng sa sinh 1,54 kg±0,23 so vai 1,38 kg±0,26, hrang iing (P<0,05) va gan CO khac biet a trgng lugng heo cai sUa 6,2 kg±0,99 so voi 5,98±1,09, tuang img (P=0,060).
S6 heo eon con s6ng den cai siia trung binh a 16 thi nghiem (bl sung ASCOGEN®) la 11,16 kg va 16 dli chimg (control) la 11,00 kg, nhung kh6ng CO su khac biet (P>0,05).
Nucleotides (ASCOGEN®) da dugc bao cao qua cac nghien ciin ve cai thien kha nang hap thu chat dinh duong, tham gia vao qua trinh phan chia cua te bao, nhat la nhung te bao cua ca the CO tinh biet hoa cao va thay the lien tiic nhu niem mac tieu hoa, sinh san va mien dich (Whitehead va ctv., 2006); nucleotides cai thien sir phat tnen ciia nhung mao rugt, tang chieu dai nhung mao rugt de lam cho dien tich be mat trong ciia rugt Ion hon va su hdp thu chdt dinh duang t6t hon (Cheng va ctv., 2011, Peng va ctv., 2013). Nang sudt nai thi hien su khac biet (P<0,05) qua chi s6 trgng lugng heo so sinh va duo'ng nhu cung c6 sir khac biet a trgng lugng heo cai sua, mac dli gia tri so sanh thing ke P>0,05 (P=0,060).
Cac chi s6 nang suat can dung lugng don vi thi nghiem nhieu hon de co the thay dugc su khac biet qua thong ke, nhung a thu nghiem nay ket qua da cai thien 0,22kg (6,20-5,98) trgng Iugng binh quSn tren mgt heo cai siia va tang them 0,16 (11,6-11,0) heo cai siia/liia c6 dugc tir thi nghiem nay ciing khong phai la con so nho, tuy nhien hieu qua kinh te so sanh can dugc tinh toan chi tiet dua tren chi s6 dau tu va thu h6i ma thi nghiem nay chua the hien.
IV. K E T L U A N
B6 sung ASCOGEN® vao khau phan cua heo nai mang thai 6 mgt trai heo thuong pham cho thay CO hieu qua cai thien dap iing mien dich ciia vacxin djch ta heo sau tiem phong, nhat la keo dai mien dich thu dgng (MDA) va kich thich mien dich tot hon tren heo con tiem phong. Heo con dugc sii dung ASCOGEN® vao thirc an tap an Cling giiip tang dap irng mien dich ciia vacxin sau tiem phong PRRS va dich ta heo. Ngoai ra, heo nai co sir dung ASCOGEN® ciing cho ket qua cao hon ve trgng lugng heo con so sinh va cai siia ciia chimg so voi heo nai kh6ng sir dung che pham.
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