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Fig. 1. Mn induces neurite outgrowth in PC12 cells grown in the absenceof NGF and this activity is significantly inhibited by function-blockingPC12 cells grown in the absence of Mn exhibit a rounded morphologywith very few neuritic extensions
Fig.3. Immunoprecipitationofintegrinsubunitsfromsurfacebiotinylated PC12 cells. Anti-ECMR serum (lane 1) immunoprecipitatesintegrin subunits whose electrophoretic properties are similar to thoseimmunoprecipitated by polyclonal aintegrin antiserum (lane 2) andanti-vamAb (lane 3).v
Fig. 5. Characterization of a stable clone of PC12 cells (nrPC12) thatdoes not respond to the neurite-promoting effects of Mn
Fig. 8. Mn-induced neurite outgrowth requires de novo mRNA andprotein synthesis. Addition of actinomycin D (ACT-D, 0.1(Pindicate which data sets are significantly different from the set repre-cycloheximide (CHX, 1.0significantly inhibits the percentage of ce

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