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Lampiran 1 Uji patogenesis cendawan endofit. A, Benih yang ditanamn dalam media PDA tanpa dan dengan cendawan endofit; B, Screening cendawan patogenik dan non patogen (Gambar atas berpotensi non patogen dan gambar bawah berpotensi patogen); C, panjang bibit cabai (ujung kiri tanpa cendawan selainnya dengan cendawan endofit)
A
Lampiran 1 Lanjutan…
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Lampiran 2 Identifikasi Isolat-isolat Non-patogenik
Kode Isolat Identifikasi Mikroskopis Identifikasi Molekuler
C9 . Hifa steril Guignardia magifera (kemiripan 99%) C23 Hifa steril Cercospora nicotianae (kemiripan 100%) C1 . Hifa steril Pleosporaceae (kemiripan 98%) H12 Curvularia sp
Tidak dilakukan identifikasi molekuler
C8
. Hifa steril
Dothideomycete sp
Lampiran 3 Identifikasi kutukebul Bemisia tabaci
Caudal seta
Caudal furrow Eight abdominal seta
Vasiform orifice Sub marginal area Thoracic tracheal comb Thoracic tracheal fold
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Lampiran 4 Isolat PepYLCV dan gejala yang ditimbulkan. A, Sumber inokulum virus dari lapang; B, Inokulum diperbanyak pada tanaman tomat varietas Ratna; C, Gejala yang ditimbulkan ketika PepYLCV ditularkan pada cabai TM 888
A B
Lampiran 5 Kriteria skoring gejala pada waktu 1 bulan setelah inokulasi. A; Tanpa gejala; B; gejala skor 1; C; gejala skor 2; D; gejala skor 3; E; skor gejala 4; F; skor gejala 5
A B
C D
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Lampiran 6 Hasil sekuensing isolat virus kuning Yogyakarta.
1 CTCTAGTGAC ATCGGAAACG CAAAGGACCT TCCCAGTATG TGTTACGTCA 50
TGTCTCTGTT CAAAGGATTG GACCTTACAA GGTCCTTCAC AACCCCGTGG 100
101 AACATCACTA GTCCTTCGAC CCCTGTAGAA TCTGGGTTTC CGATTCATAG 150
151 GTCGATTTAC CCATGCTCTT CGTTTGTAAG ACATGCCTGG GGCAGTGGGG 200
201 GCAGCAGCAG GGAGACTGTA CTGACTCGCG TAGTTTAGTC TCCGGCGTGT 250
251 TATAGACATT GGCAACGACG ACACTGTATC GATGGAACGC TTCGGCATAG 300
301 TTTTTACAAC GCAACAGGCA GATTAAATCC TTCACAAATT CACCTCCGAC 350
351 CGTATCAGGA GAATACGTAG CCTCTAAGCT TTGCAGATAT TTGATTGCCA 400
401 ACATGCATCG AAACCCGTGT AGGGTTTCTG GAAAAGGATG AACGAGCGGA 450
451 TCCCACATTC TTGGGTAGTG GAGAGTGGTC CCTATCTTTA TAGTGGGCGA 500
501 CCCACTAATT AAGCTTTTCA GAAGCAACGC CATTGGTCAG TCAAAAGGTT 550
551 GGCTCGTGGG ACCACCTTTA AAAAATTTTC GCGGCACTCG GTAATATTAT 600
601 ACGAGTGCCG CTTTACCCGG GTAAGGCCCA ATAAGTACTA AGGCCCATAT 650
651 AGAACTTCTA TATATTGTCT CCTGTCTCCA ATATAGGGGG ATAGAAGGAT 700
701 TGTCTCCAAT ACAAAACGAC ACTCCAAATG CCTCCACCAC GTCGTTTTAA 750
751 GTTACAGAGT AAAAACTATT TCTTAACATA TCCACACTGT TCTCTAACCA 800
801 AAGAAGAAGC ACTCGAACAA TTAAAATCCA TCAACACTCC TGTCAACAAG 850
851 CTGTTTGTCA AGATCTGTAG AGAACTTCAC GAAGATGGGA GCCCTCATCT 900
901 CCATGTGCTC ATACAATTCG AAGGGAAATA CGTCTGCACG AACAACAGAT 950
951 TCTTCGACCT GGTATCCCCA ACCAGGTCAG CACATTTCCA TCCGAACATT 1000
1001 CAGGGAGCTA AATCAAGTTC CGATGTCAAG GCGTACATGG ACAAAGACGG 1050
1051 TGATACAACC GAATGGGGAG AATTTCAGAT AGATGGCCGA TCTGCACGAG 1100
1101 GTGGTCAGCA CGCAGTAAAC GATGTTTACG CGCAGGCCCT AAATTGTGGA 1150
1151 AGTAAGTCCG ATGCTCTTCA ATTGATTAAA GAGCTTGCTC CGAAAGACTA 1200
1201 TGTTCTACAG TATCACAATT TAAGTGTTAA TTTCGATAAA ATATTTGTAA 1250
