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Lampiran 1 Manajemen pemberian pakan pada media pemeliharaan Minggu ke- Target SR(%) Jumlah (Ekor) ABW awal (G) Target ABW (G) FR (%) F/Day Target Biomassa (G) 1 100% 30 2.0 3.0 8.0% 7.2 90.0 2 98% 29 3.0 4.0 7.0% 8.2 117.6 3 96% 29 4.0 5.0 6.0% 8.6 144.0 4 94% 28 5.0 6.0 5.5% 9.3 169.2 5 92% 28 6.0 7.0 5.0% 9.7 193.2 6 90% 27 7.0 8.0 4.5% 9.7 216.0
Lampiran 2 Tabel Feed Rate (FR) pada udang vaname (Litopenaeus vannamei) Average Shrimp Wt. (g) Feed Rate (%bw/ Day)
<1 35 - 25 0.1 - 0.24 25 - 20 0.25 - 0.49 20 - 15 0.5 - 0.9 15 - 11 1.0 - 1.9 11 - 8 2.0 - 2.9 8 - 7 3.0 - 3.9 7 - 6 4.0 - 4.9 6 - 5.5 5.0 - 5.9 5.5 - 5.0 6.0 - 6.9 5.0 - 4.5 7.0 - 7.9 4.5 - 4.25 8.0 - 8.9 4.25 - 4.0 9.0 - 9.9 4.0 - 3.75 10.0 - 10.9 3.75 - 3.5 11.0 - 11.9 3.5 - 3.0 12.0 - 12.9 3.25 - 3.0 13.0 - 13.9 3.0 - 2.75 14.0 - 14.9 2.75 - 2.5 15.0 - 15.9 2.5 - 2.3 16.0 - 16.9 2.3 - 2.1 17.0 - 17.9 2.1 - 2.0 18.0 - 18.9 2.0 - 1.9 19.0 - 19.9 1.9 - 1.8 20.0 - 20.9 1.8 - 1.7 (Van wyk, 1999).
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Lampiran 3 Karakterisasi sifat fisiologi dan biokimia bakteri Uji Oxidatif/ Fermentatif
1. Siapkan peralatan berupa jarum ose, bunsen dan botol semprot 2. Tulis jenis bakteri pada media agar miring
3. Lakukan proses sterilisasi dengan menyemprot kedua tangan serta tempat yang akan digunakan untuk melakukan proses pewarnaan.
4. Lakukan pembakaran jarum ose sebelum mengambil sampel bakteri. Pada saat pengambilan sampel lakukan dekat dengan api.
5. Isolat diambil dengan jarum ose dan ditusukkan pada media tumbuh hingga masuk ke dalam media tumbuh 2/3 bagiannya.
6. Seluruh media yang telah diujikan pada media agar miring dan telah diinokulasikan bakteri contoh selanjutnya diinkubasi didalam inkubator selama 24 jam.
Uji Motilitas
1. Siapakan peralatan berupa jarum ose, bunsen dan botol semprot. 2. Tulis jenis bakteri pada objek glass.
3. Lakukan proses sterilisasi dengan menyemprot kedua tangan serta tempat yang akan digunakan untuk melakukan uji motilitas.
4. Lakukan pembakaran jarum ose sebelum mengambil sampel bakteri. Pada saat pengambilan sampel lakukan dekat dengan api.
5. Isolat yang telah diambil dengan jarum ose kemudian ditusukkan kedalam media SIM.
6. Seluruh media yang telah diujikan pada media agar miring dan telah diinokulasikan bakteri contoh selanjutnya diinkubasi didalam inkubator selama 24 jam.
Uji Oksidase
1. Pada uji oksidase ini sebelumnya disiapkan cuplikan kertas saring yang telah diteteskan dengan p-aminodimethylaniline-oxalat 1% pada objek glass.
2. Sejumlah bakteri diambil dari agar miring dan diletakkan di atas cuplikan kertas saring.
3. Proses selanjutnya adalah mengamati perubahan warna yang terjadi pada tetesan larutan p-aminodimethylaniline-oxalat. Indikator warna merah menunjukkan tes positif dan bila berwarna ungu tes negatif.
Uji Katalase
1. Objek glass dibersihkan dengan alkohol dan dilewatkan beberapa kali pada nyala api Bunsen.
2. Teteskan senyawa H2O2 hingga seluruh bagian bakteri terendam.
3. Sejumlah bakteri diambil dari media agar miring kemudian ditempelkan pada objek glass yang telah ditetesi senyawa H2O2.
4. Pengamatan dilakukan terhadap kemampuan bakteri melawan efek dari senyawa H2O2 dengan indikator terciptanya buih dan/atau busa. Apabilah busa terbentuk maka menunjukkan tes positif sedangkan bila tidak terbentuk busa dan hanya buih tes dianggap negatif.
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Lampiran 4 Hasil karakterisasi morfologi, fisiologi dan biokimia bakteri bioflok
Jenis Bentuk
Hasil Uji
Gram O/F SIM Katalase Oksidase
Alcaligenes Batang - + + + Negatif
Acinetobacter Bulat - - + - Negatif
Actinobacillus Batang F - + + Negatif
Bacillus Batang - + + + Positif
Eikenella Batang - - - + Negatif
Enterobacteria Batang F - - + Negatif
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Lampiran 5 Prosedur penambahan Sumber karbon
Keterangan :
1) Untuk mengetahui kandungan protein serta C dalam pakan dilakukan dengan uji proksimat.
2) Untuk mengetahui kandungan C organik dalam sumber karbon dilakukan uji C organik.
FR (%) x Biomassa (gr)
Persamaaan 1 x Kandungan protein (%)
Persamaan 2 x 16 % (Jumlah N dalam pakan)
Persamaan 1 Persamaan 2 Persamaan 3 Persamaan 3 x 90 % (Jumlah N terbuang ke perairan)
Persamaan 4 x C/N rasio yang ditentukan
Persamaan 4
Persamaan 5 Persamaan 5 x Kandungan C
organik dalam sumber karbon (%)
Persamaan 6 Persamaan 6 – kandungan C
organik dalam pakan (%)
Jumlah karbon yang harus ditambahkan
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