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LAMPIRAN
Lampiran 1. Komposisi Media dan Reagen a. Media Ashby’s Agar
Manitol 20 gram K2HPO4 0,2 gram MgSO4.7H2O 0,2 gram NaCl 0,2 gram
K2SO4 0,1 gram CaCO3 0,5 gram Agar 20 gram Akuades 1000 mL
b. Media Ashby’s Broth Manitol 20 gram K2HPO4 0,2 gram MgSO4.7H2O 0,2 gram NaCl 0,2 gram
K2SO4 0,1 gram CaCO3 0,5 gram Akuades 1000 mL
c. Media Ashby’s Semi Padat Manitol 30 gram
K2HPO4 0,3 gram MgSO4.7H2O 0,3 gram NaCl 0,3 gram
K2SO4 0,15 gram CaCO3 0,75 gram Agar 15 gram Akuades 1500 L
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Lanjutan Lampiran 1 d. Media Kitin Agar
K2HPO4 0,7 gram KH2PO4 0,3 gram MgSO4.7H2O 0,5 gram FeSO4.7H2O 0,01 gram ZnSO4.7H2O 0,001 gram MnCl2 0,001 gram
Koloidal kitin 0,2% 66,6 mL Agar 20 gram
Akuades 1000 mL
e. Media Glukan Agar Laminarin 90% 6 gram KH2PO4 0,9 gram K2HPO4 0,39 gram NaCl 1,5 gram (NH4)2SO4 0,3 gram MgSO4. 7H2O 0,072 gram Ekstrak kamir 0,9 gram Agar bakto 12 gram Akuades 600 mL
f. Reagen Salkowski H2SO4 pekat 80 mL FeCl3. 6H2O 0,1 M 4 mL Akuades 116 mL
g. Larutan MacFarland BaCl2 1% 0,5 mL H2SO4 1% 9,5 mL Akuades 10 mL
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Lampiran 2. Kurva Standar a. Kurva standar IAA
No. Konsentrasi IAA (ppm)
Nilai Absorbansi
1. 0 0,017
2. 5 0,042
3. 10 0,134
4. 15 0,197
5. 20 0,245
6. 25 0,257
7. 30 0,345
y = 0,0109x + 0,0133 R² = 0,9735
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4
0 5 10 15 20 25 30 35
Absorbansi
Konsentrasi IAA (ppm)
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Lanjutan Lampiran 2
b. Kurva Standar Nitrogen
No. Konsentrasi NH4Cl (ppm)
Nilai Absorbansi
1. 0 0
2. 2 0,275
3. 4 0,450
4. 6 0,631
5. 8 0,712
6. 10 0,853
y = 0,1645x - 0,0889 R² = 0,9686
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
0 2 4 6 8
Absorbansi
Konsentrasi NH4Cl (ppm)
39
Lampiran 3. Dokumentasi Penelitian
a. Hasil Isolasi Bakteri Diazotrof dari Humus Kelapa Sawit Perkebunan Universitas Sumatera Utara (A), Kebun Rakyat Desa Bingkat (B) dan PTPN IV Adolina (C) pada Media Ashby’s Agar Umur Inkubasi 24 Jam pada Suhu 28˚C
b. Uji Sitrat Bakteri Diazotrof Humus Kelapa Sawit pada Media Simon Citrate Agar Umur Inkubasi 24 Jam pada Suhu 28˚C
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Lanjutan Lampiran 3
c. Uji Motil Bakteri Diazotrof Humus Kelapa Sawit pada Media Sulfide Indole Motility Umur Inkubasi 24 Jam pada Suhu 28˚C
d. Uji Katalase Bakteri Diazotrof Humus Kelapa Sawit
e. Pelikel Isolat Bakteri Diazotrof dari Humus Kelapa Sawit Perkebunan Universitas Sumatera Utara, Kebun Rakyat Desa Bingkat dan PTPN IV Adolina pada Media Ashby’s Semi Padat Umur 10 Hari pada Suhu 28˚C
Pelikel yang terbentuk
41
Lanjutan Lampiran 3
f. Uji Kualitatif Isolat Bakteri Diazotrof dari Humus Kelapa Sawit Perkebunan Universitas Sumatera Utara, Kebun Rakyat Desa Bingkat dan PTPN IV Adolina Pelarut Fosfat pada Media Pikovskaya Agar Umur 5 hari pada Suhu 28˚C
Keterangan : TU : Tanah USU, TP: Tanah PTPN IV.
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