6 SIMPULAN UMUM DAN SARAN Simpulan Umum
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Lampiran 1 Komposisi media PDA
Kentang 200 g/L
Dekstrosa 20 g/L
Agar 15 g/L
pH akhir (pada suhu 25oC) 5,6±0,2
Lampiran 2 Komposisi media PDB
Kentang 200 g/L
Dekstrosa 20 g/L
Agar 15 g/L
pH akhir (pada suhu 25oC) 5,1±0,2
Lampiran 3 Hasil uji statistik interaksi perlakuan konsentrasi NaCl dan waktu panen
Hipotesis
H0 (αβ)ij=0, tidak ada pengaruh interaksi konsentrasi NaCl dan waktu panen terhadap bobot biomassa kapang KT30
H1 (αβ)ij≠0, ada pengaruh interaksi konsentrasi NaCl dan waktu panen terhadap bobot biomassa kapang KT30
Yijk2 = 37,0297 Yi..2 = 785,67 Y.j.2 = 328,11 Yij.2 = 110,91 Y… = 48,41 Faktor koreksi (FK) = �…2 ��� = 48,412 3.7.3 = 32,5490 Jumlah Kuadrat Total (JKT) = ∑ ����2
�,�,� − ��
= 37,0297 – 32,5490 = 4,4807 Jumlah Kuadrat A (waktu panen) (JKA) = ∑ ��� ..2
�� − �� = 785 ,67
3.8 −32,5490 = 0.1871
Jumlah Kuadrat B (NaCl) (JKB) = ∑ �.�.
2
�
�� − ��
= 328 ,11
Jumlah Kuadrat AB (JK(AB)) = ∑ ���. 2 �� � − �� − ��� − ��� = 110 ,91 3 −32,5490−0,1871−3,9081 = 0,3263
Jumlah Kuadrat Galat (JKG) = JKT – JKA – JKB – JK(AB) = 4,4807 – 0,1871 – 3,9081 – 0,3263 = 0,0593
Tabel analisis sidik ragam
Sumber Ragam db JK KT F hitung F tabel (0.05)
A 7 0,1871 0,0936 75,7672 3,1907
B 2 3,9081 0,5583 452,1640 2,2074
AB 14 0,3263 0,0233 18,8737 1,9037
Galat 48 0,0593 0,0012
Total 71 4,4807
F hitung > F tabel (tolak H0) berarti interaksi konsentrasi NaCl dan waktu panen berpengaruh nyata terhadap biomassa kapang KT30
Lampiran 4 Hasil uji statistik perbedaan saturasi amonium sulfat
Hipotesis
H0 (αβ)ij=0, tidak ada pengaruh perbedaan saturasi amonium sulfat terhadap rendemen pelet protein kapang KT30
H1 (αβ)ij≠0, ada pengaruh perbedaan saturasi amonium sulfat terhadap rendemen pelet protein kapang KT30
Yij2 = 31,1575 Yi.2 = 93,29 Y.j2 = 124,4243 Y.. = 19,31 Faktor koreksi (FK) = �..2 �� = 19.312 3.4 = 31,0730 Jumlah Kuadrat Total (JKT) = ∑ ���2
�,� − ��
= 31,1575 – 31,0730 = 0,0845 Jumlah Kuadrat Perlakuan = ∑ ��� .2
� − �� = 93,29
3 −0,0845 = 0,0243
Jumlah Kuadrat Sisa (JKS) = JKT – JKP = 0,0845 – 0,0243 = 0,0602
Tabel analisis sidik ragam
Sumber Ragam db JK KT F hitung F tabel (0.05)
Perlakuan 3 0,0243 0,0082 1,0845 4,0662
Sisa 8 0,0602 0,0075
Total 11 0,0845
F hitung < F tabel (gagal tolak H0) berarti perbedaan saturasi amonium sulfat tidak berpengaruh nyata terhadap rendemen pelet protein kapang KT30
Lampiran 5 Perhitungan nilai IC50
Berdasarkan tabel diatas, maka diperoleh persamaan linier berikut:
Lampiran 6 Kurva standar Bovine Serum Albumin (BSA)
Berdasarkan hasil pengukuran BSA, maka diperoleh kurva standar berikut: y = 0,035x + 26,52 R² = 0,908 0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 0 200 400 600 800 1000 1200 D aya H am b at (% ) Konsentrasi Protein (ppm) y = 0,555x + 0,138 R² = 0,972 0 0.2 0.4 0.6 0.8 1 1.2 1.4 0 0.5 1 1.5 2 2.5 Abso r ban si ( nm ) Konsentrasi (mM)
Lampiran 7 Perhitungan bobot molekul
Berdasarkan tabel diatas, maka diperoleh persamaan linier berikut:
Lampiran 8 Aktivitas antibakteri supernatan kapang KT30
Konsentrasi NaCl : 0% Bakteri : Bacillus subtilis
Konsentrasi NaCl : 0% Bakteri : Escherichia coli
Konsentrasi NaCl : 3% Bakteri : Bacillus subtilis
Konsentrasi NaCl : 3% Bakteri : Escherichia coli
y = -1,919x + 1,876 R² = 0,962 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 0.00 0.10 0.20 0.30 0.40 0.50 0.60 Lo g B M Nilai Rf K 21 18 15 12 9 6 3 12 15 18 21 K 9 6 3 3 21 18 15 12 9 6 K 21 18 15 12 9 6 3 K
Lampiran 9 Perhitungan konsentrasi protein per disc Kadar protein = 1,697 mg/mL
Volume protein per disc = 20 µL
Konsentrasi protein/disc = ��µ�� �.�����
��� � �������µ� � �������µ�