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Lampiran 1 Komposisi Media Murashige-Skoog (MS) Kode Senyawa Berat yang di
Timbang (gram) Dilarutkan dalam akuades (ml) A NH4NO3 82.5 1000 B KNO3 95 1000 C CaCl2.2H2O 22 250 D H3BO3 0.31 250 KH2PO4 8.5 CoCl2.6H2O 0.0013 Na2MoO4.2H2O 0.0125 KI 0.0415 E MgSO4.7H2O 18.5 250 MnSO4.4H2O 0.7527 ZnSO4.7H2O 0.43 CuSO4.7H2O 0.0013 F Na-EDTA 1.86 250 FeSO4.7H2O 1.39 Vit. B5 Myo-inositol 1 100 Thiamine-HCl 0.1 Nicotinic acid 0.01 Pyridoxine-HCl 0.01 Sukrosa 30 1000 Gellan gum 3 1000
Lampiran 2 Nilai slope untuk validitas Quantitative Real Time PCR Sampel pengenceran RNA ΔCt (Actin-B11) ΔCt (Actin-STOP1) ΔCt (Actin-ALMT1) ΔCt (Actin-MATE) Slope (Actin-B11) Slope (Actin-STOP1) Slope (Actin-ALMT1) Slope (Actin-MATE) TL.U1 1x - -0.01 2.29 1.28 - 0.001 0.000 0.000 2x 0.3 2.23 1.28 10x 0.09 2.3 2.37 100x 0.23 2.39 1.9 1000x 1.4 2.98 1.36 TL.U2 1x - -0.01 1.93 1.27 - 0.000 0.000 0.000 2x -0.01 1.96 0.56 10x 0.69 2.79 1 100x 0.76 1.81 1.95 1000x -0.09 1.96 1.32 TL.U3 1x - 0.65 3.05 1.78 - 0.000 0.001 0.000 2x 0.88 3.35 1.23 10x 0.99 3.48 1.81 100x 1.07 3.95 1.78 1000x 0.75 4.93 1.82
TL.U1= tanaman tipe liar ulangan biologi 1 TL.U2= tanaman tipe liar ulangan biologi 2 TL.U3= tanaman tipe liar ulangan biologi 3
Sampel pengenceran RNA ΔCt (Actin-B11) ΔCt (Actin-STOP1) ΔCt (Actin-ALMT1) ΔCt (Actin-MATE) Slope (Actin-B11) Slope (Actin-STOP1) Slope (Actin-ALMT1) Slope (Actin-MATE) TL.U1+Al 1x - -2.3 0.07 0.37 - 0.000 0.001 0.000 2x -2.48 0.16 0.55 10x -3 0.52 0.97 100x -1.99 0.17 0.45 1000x -2.35 1.19 0.87 TL.U2+Al 1x - -2.49 0.05 -0.98 - 0.000 0.000 0.000 2x -2.65 -0.07 -1.09 10x -2.47 0.02 -0.13 100x -2.09 0.91 -0.71 1000x -3.05 0.04 -0.25 TL.U3+Al 1x - -1.22 0.11 0.07 - 0.000 0.001 0.000 2x 0.09 0.14 0.12 10x 0.08 0.2 0.39 100x -1.19 0.78 0.12 1000x -0.21 0.62 0.29
TL.U1+Al= tanaman tipe liar ulangan biologi 1+Al TL.U2+Al= tanaman tipe liar ulangan biologi 2+Al TL.U3+Al= tanaman tipe liar ulangan biologi 3+Al
Lampiran 2 Nilai slope untuk validitas Quantitative Real Time PCR Lanjutan Sampel pengenceran RNA ΔCt (Actin-B11) ΔCt (Actin-STOP1) ΔCt (Actin-ALMT1) ΔCt (Actin-MATE) Slope (Actin-B11) Slope (Actin-STOP1) Slope (Actin-ALMT1) Slope (Actin-MATE) T.U1 1x 2.7 -1.09 0.9 0.91 0.000 0.000 0.000 0.000 2x 2.91 -1.61 -0.02 0.73 10x 2.34 -1.79 0.74 0.6 100x 1.9 -1.22 0.64 0.75 1000x 2.75 -1.18 1 0.95 T.U2 1x 3.21 -1.48 0.43 0.59 0.000 0.000 0.001 0.000 2x 3.93 -2.08 0.78 0.64 10x 3.71 -1.94 0.61 0.53 100x 3.41 -0.86 0.89 0.78 1000x 3.47 -1.81 1.13 1 T.U3 1x 2.99 -2.04 1.22 0.51 0.000 0.001 0.001 0.000 2x 3.01 -1.61 1.5 0.89 10x 3.21 -1.68 0.69 0.73 100x 2.29 -2.47 1.39 0.87 1000x 2.37 -1.68 1.57 0.9
T.U1= tanaman transgenik ulangan biologi 1 T.U2= tanaman transgenik ulangan biologi 2 T.U3= tanaman transgenik ulangan biologi 3
T.U1+Al 1x 4.47 -3.77 -1.34 -1.87 0.000 0.000 0.002 0.000 2x 4.72 -3.68 -1.16 -1.92 10x 4.83 -3.9 -1.26 -1.34 100x 4.12 -2.59 -1.94 -1.28 1000x 4.46 -3.84 1.06 -1.06 T.U2+Al 1x 5.02 -3.17 -1.3 -1.99 0.000 0.001 0.002 0.001 2x 5.37 -2.93 -1.4 -2.13 10x 5.07 -3.05 -1.61 -1.31 100x 5.91 -4.94 -1.3 -2.18 1000x 4.64 -3.19 -1.4 -1.17 T.U3+Al 1x 5.58 -3.42 -1.3 -1.95 0.001 0.001 0.001 0.002 2x 6.97 -3.21 -1.2 -3.81 10x 5.87 -4.93 -1.31 -3.99 100x 5.71 -1.92 -1.45 -3.81 1000x 5.14 -2.27 -0.34 -0.85
T.U1+Al = tanaman transgenik ulangan biologi 1+Al T.U2+Al = tanaman transgenik ulangan biologi 2+Al T.U3+Al = tanaman transgenik ulangan biologi 3+Al
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