Kesimpulan
Bakteri asam laktat asal bekasam (SK(5), BP(10), dan NS(9)) memiliki sifat sebagai kandidat probiotik, yaitu memiliki ketahanan hidup yang tinggi (>50%) pada pH lambung (pH 2,0), pH usus (pH 7,2), dan pH usus (pH 7,2) dengan
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garam empedu 0,5%, serta memiliki aktivitas antimikrob terhadap bakteri patogen makanan (EPEC, Staphylococcus aureus, dan Salmonella typhimurium ATCC
14028). Isolat BAL kandidat probiotik termasuk Gram-positif dengan bentuk
batang, tidak motil, katalase negatif, dan homofermentatif. Isolat SK(5) dan NS(9) adalah Lactobacillus plantarum 1 dengan kemiripan 99,9% dan 99,5%, sedangkan BP(10) adalah Lactobacillus pentosus dengan kemiripan 99,9%.
Saran
Karakteristik probiotik asal bekasam dalam saluran pencernaan manusia perlu dilakukan, meliputi uji kemampuan penempelan bakteri asam laktat terpilih pada sel epitel usus dan uji koagregasi. Pengujian keamanan secara in vivo dari bakteri asam laktat asal bekasam perlu dilakukan untuk membuktikan bahwa bakteri asam laktat asal bekasam yang telah diperoleh aman dikonsumsi oleh manusia.
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DAFTAR PUSTAKA
Arief II, Maheswari RRA, Suryati T, Hidayati . 2006. Karakteristik Lactobacillus spesies yang diisolasi dari daing sapi. Seminar Nasional Teknologi
Peternakan dan Veteriner 2006. 861-865.
Argyri AA, Zoumpopoulou G, Karatzas GKA, Tsakalidou E, Nychas EGJ, Panagou EZ, Tassou CC. 2013. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiology. 33(2013):282-291.
Bao Y, Zhang Y, Liu Y, Wang S, Dong X, Wang Y, Zhang H. 2010. Screening of potential probiotics properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control. 21 : 695-708.
Begley M, Hill C, Grahan CGM. 2006. Bile salt hydrolase activity in probiotics.
Applied and Environmental Microbiology. 72(3):1729-1738.
Bogaert JC, Naidu AS. 2000. Lactic Acid, Natural Food Antimicrobial System. Florida (US): CRC Press.
Bourlioux P, Koletzko B, Guarner F, Braesco V. 2003. The intestine and its microflora are partners for the protection of the host : report on the Danone symposium ‘ The Intelligent Intestine’, held in Paris, June 14, 2002.
American Journal of Clinical Nutrition. 78:675-683.
Budiman C. 2004. Karakteristik kimiawi dadih susu sapi hasil fermentasi bakteri probiotik yang disimpan pada suhu berbeda. [skripsi]. Bogor (ID): Program Studi Teknologi Hasil Ternak, Departemen Ilmu Produksi Terak, Fakultas Peternakan, Institut Pertanian Bogor.
Cappuccino JG, Sherman N. 1983. Microbiology A Laboratory Manual. New York (US): State University of New York. Hlm: 123.
Charteris WP, Kelly PM, Morelli L, Collins JK. 1998. Ingredient selection criteria for probiotic microorganisms in functional dairy foods. International
Journal Dairy Technology. 51(4):123-135.
Chou LS, Weimer B. 1999. Isolation and characterization of acid and bile tolerant isolates from strains of Lactobacillus acidophilus. Journal Dairy Science. 62:23-31.
Cotter PD, Hill C. 2003. Surviving the acid test : responses of Gram-positive bacteria to low pH. Microbiology and Molecular Biology Reviews. 67:429-453.
Counter M et al. 2005. Characterization of lactic acid bacteria isolated from Italian dry fermented sausage. Annual Faculty Medicine and Veterinay. 27:167-174.
De Angelis MA, Corsetti N, Tosti J, Rossi MR, Corbo, Gobbetti M. 2001. Characterization of non-starter lactic acid bacteria from Italian ewe cheeses based on phenotypic, genotypic, and cell wall protein analyses. Applied
Environmental Microbiology. 67:2011-2020.
Desniar. 2012. Karakterisasi bakteri asam laktat dari produk fermentasi ikan (bekasam). [disertasi]. Bogor (ID): Sekolah Pascasarjana, Institut Pertanian Bogor.
22
Diop MB, Dubois-Dauphin E, Tine A, Ngom J, Destain, Thonart P. 2007. Bacteriocin producers from traditional food products. Biotechnology,
Agronomy, Society and Environment. 11:275-281.
Djaafar TF. 1997. Bakteri asam laktat dan manfaatnya sebagai pengawet makanan. Jurnal Penelitian dan Pengembangan Pertanian. 16(1):19-22. Doores S. 1983. Organic Acids, Antimicrobial in Foods. New York (US): Marcel
Dekker.
FAO [Food And Agriculture Organization], WHO [World Health Organization]. 2006. Guidelines for the evaluation of probiotics in food. Report of Joint
FAO/WHO Working Group on drafting Guidelines for the evaluation of probiotics in food. Rome (IT): Food And Agriculture Organization and
World Health Organization.
Gálvez A, Abriouel H, López RL, Omar NB. 2007. Bacteriocin-based strategies for food biopreservation. International Journal Food Microbiology. 120:51– 70.
Guerra A, Etienne-Mesmin L, Livrelli V, Denis S, Blanquet-Diot S, Alric M. 2012 Relevance and challenges in modeling human gastric and small intestinal digestion. Trends in Biotechnology. 30(11):591-600.
Hatakka K, Savilahti E, Ponka A, Meurman JH, Poussa T, Nase L, Saxelin M, Korpela R. 2001. Effect of long term consumption of probiotic milk on infections in children attending day care centres: double blind, randomised trial. British Medical Journal. 322(7298):1327.
Hamza AA, Gaali EIE, Mahdi AA. 2009. Use of the RAPD-PCR fingerprinting and API system for clustering lactic acid bacteria isolated from traditional Sudanese sour milk (Roab). African Journal of Biotechnology. 8(15):3399-3404.
Havenaar R, Huis IVJHJ. 1992. Selection of Strains for Probiotic Use, Probiotics:
The Scientific Basic. London (UK): Chapman & Hall.
Hayward AC. 1957. Detection of gas production from glucose by heterofermentative lactid acid bacteria. Journal of General Microbiology. 16:9-15.
Herich R, Levkut M. 2002. Lactic acid bacteria, probiotics and immune system.
Journal Veterinarni Medicina-Czech. 47:169-180.
Hill M.J. 1995. Role of Gut Bacteria in Human Toxicology and Pharmacology. New York (US): Taylor.
Hugas M. 1998. Bacteriocinogenic lactic acid bacteria for the biopreservation meat and meat products. Meat Science. 49:S139-S150.
Hutkins RW, Nannen NL. 1993. pH homeostatis in lactic acid bacteria. Journal
Dairy Science. 76:2354-2365.
Hwanlem N, Buradaleng S, Wattanachant S, Benjakul S, Tani A, Maneerat S. 2011. Isolation and screening of lactic acid bacteria from Thai traditional fermented fish (Plasom) and production of Plasom from selected strains.
Food Control. 22:401-407.
Kim HJ, Shin HS, Ha WK, Yang HJ, Lee SW. 2006. Characterization of lactic bacterial strains isolated from raw milk. Journal Animal Science. 19(1):131-136.
Kimoto-Nira H, Mizumachi K, Nomura M, Kobayashi M, Fujita Y, Okamoto T, Suzuki I, Tsuji NM, Kurisaki J, Ohmomo S. 2007. Lactococcus sp. as
23 potential probiotic lactic acid bacteria. Japan Agricultural Research
Quarterly. 41:181-189.
Klaenhammer TR, Azcarate-Peril MA, Altermann E, Barrangou R. 2011. Influence of the dairy environtment of gene expression and substrate utilization in lactic acid bacteria. Journal of Nutrition. 137:748-750.
Lin WH, Hwang CF, Chen LW, Tsen HY. 2006. Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Journal Food
Microbiology. 23: 74-81.
Mitsuoka T. 1990. Bifidobacteria and their role in human health. Journal Indian
of Microbiology. 6:263–268.
Mozzi F, Raya RR, Vignolo GM. 2010. Biotechnology of Lactid Acid Bacteria:
Novel Applications. London (UK): Blackwell Publishing, Ltd. Hlm: 3-5.
Nitisinprasert S, Pungsungworn N, Wanchaitanawong P, Loiseau W, Montet D. 2006. In vitro adhesion assay of lactic acid bacteria, Escherichia coli and
Salmonella sp. by microbiological and PCR methods. Journal Science Technology. 28:99-106.
Nuraida L, Susanti, Palupi NS, Hana, Bastomi RR, Priscilia D, Nurjanah S. 2012. Evaluations of probiotics of lactic acid bacteria isolated from breast milk and their potency as starter culture for yoghurt fermentation. International
Journal of Food, Nutrition & Public Health. 5(1):33-60.
Ostergaard A, Ben EPK, Yamprayoon J, Wedel-Neergaard C, Huss HH, Gram L. 1998. Fermentation and spoilage of som-fak a Thai low-salt fish product.
Tropical Science. 38:105-112.
Pan X, Chen F, Wu T, Tang H, Zhao Z. 2009. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control. 20:598-602.
Rahayu SE. 2011. Tantangan dan peluang mengembangkan probiotik pada produk non-susu. Food Review. April 2011.
Reque EF, Pandey A, Franco SG, Soccol CR. 2000. Isolation, identification and physiological study of Lactobacillus fermentum LPB for use as probiotic in chickens. Brazil Journal of Microbiology. 31 : 303-307.
Roberfroid MB. 2000. Prebiotics and probiotics: are they functional foods?.
American Journal of Clinical Nutrition. 71:1682S-1687S.
Ross RP, Morgan S, Hill C. 2002. Preservation and fermentation: past, present and future. Int J Food Microbiol. 79:3-16.
Savadogo A, Outtara CAT, Bassole IHN, Traore AS. 2004. Antimicrobial activities of lactic acid bacteria strains isolated from Burkina Faso fermented milk. Pakistan Journal of Nutrition. 3:174-179.
Sunny-Roberts EO, Knoor D. 2008. Evaluation of the response of Lactobacillus
rhamnosus VTT E-97800 to sucrose-induced osmotic stress. Food Microbiology. 25:183-189.
Svensson U. 1999. Industrial perspective. Probiotics: A Critical Review. 15(8):57-64.
Theron MM, Lues JFR. 2011. Organic Acids and Food Preservation. Florida (US): CRC Press. Hlm: 273.
Tiwari RP, Hoondal GS, Tewari R. 2009. Laboratory Techniques in Microbiology
24
Tuomola E, Crittenden R, Playne M, Isolauri E, Salminen S. 2001. Quality assurance criteria for probiotic bacteria. American Journal of Clinical
Nutrition. 73:393S-398S.
Vamanu A, Vamanu E, Drugulescu M, Popa O, Campeanu G. 2006. Identification of lactic bacterium galur used for obtaining a pollen-based probiotic product. Turkey Journal of Biology. 30:75-80.
Vassu T et al. 2001. Biochemical and genetic characterization of Lactobacillus
plantarum cmgb-1 galur used as probiotic. Roum Biotechnology. 7:585-598.
Vinderola CG, Reinheimer JA. 2003. Lactic acid starter and probiotic bacteria: a comparative ‘‘in vitro’’ study of probiotic characteristics and biological barrier resistance. Food Research. International. 36(2003):895–904.
Yang YSM, Chen C, Liao CC. 2001. Mutant bifidobacteria strains with acid, bile salt and oxygen tolerance. United State Patent Applied. 6306638.
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LAMPIRAN
Lampiran 1 Hasil pengujian antimikrob terhadap Staphylococcus aureus Kode Isolat Diameter zona hambat (mm) x±std
SA (Ulangan 1) SA (Ulangan 2)
SK5 9 9 9,00±0,00
BP10 9 9 9,00±0,00
NS9 6 7 7,00±0,71
Lampiran 2 Hasil pengujian antimikrob terhadap Salmonella typhimurium
ATCC 14028
Kode Isolat Diameter zona hambat (mm) x±std ST (Ulangan 1) ST (Ulangan 2)
SK5 8 7 7,00±0,71
BP10 6 6 6,00±0,00
NS9 6 6 6,00±0,00
Lampiran 3 Hasil pengujian antimikrob terhadap Enteropathogenic E. coli (EPEC)
Kode Isolat Diameter zona hambat (mm) x±std
EPEC (Ulangan 1) EPEC (Ulangan 2)
SK5 6 7 7,00±0,71
BP10 7 6 7,00±0,71
NS9 5 6 6,00±0,71
Lampiran 4 Hasil uji aktivitas antimikrob terhadap bakteri patogen makanan
Staphylococcus aureus (NS(9)) EPEC (NS (9)) EPEC (SK (5) dan BP(10)) Staphylococcus aureus (SK(5) dan BP(10))
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Lampiran 5 APIWEB Biomerieux identifikasi NS(9)
Lampiran 6 APIWEB Biomerieux identifikasi BP(10)
S. typhimurium (SK (5) dan BP (10)) S. typhimurium
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RIWAYAT HIDUP
Penulis dilahirkan di Lamongan pada tanggal 8 Mei 1991 dari ayah Drs. Miftahul Arif dan ibu Dra. Luluk Maskanah. Penulis adalah putri pertama dari dua bersaudara. Tahun 2009 penulis lulus dari SMA Negeri 2 Lamongan dan pada tahun yang sama penulis diterima di Institut Pertanian Bogor sebagai mahasiswa Departemen Teknologi Hasil Perairan, Fakultas Perikanan Ilmu Kelautan, melalui jalur Undangan Seleksi Masuk Institut Pertanian Bogor (USMI).
Selama masa perkuliahan, penulis aktif dalam berbagai organisasi kemahasiswaan seperti Badan Eksekutif Mahasiswa (BEM) Fakultas Perikanan dan Ilmu Kelautan sebagai staff Departemen Sosial Lingkungan periode 2010-2011, Ketua Divisi Sosial Kemahasiswaan dan Peduli Pangan (SKPP) Himpunan Profesi Hasil Perikanan (Himasilkan) periode 2011-2012, dan anggota Fisheries Processing Club (FPC) periode 2011-2012. Penulis pun aktif menjadi panitia dalam berbagai kegiatan kemahasiswaan di Institut Pertanian Bogor, di antaranya adalah ketua divisi acara pada Simposium Nasional Kepemudaan Perikanan dan Kelautan yang diselenggarakan oleh BEM FPIK tahun 2011. Selama kuliah, penulis pernah menjadi asisten praktikum mata kuliah Iktiologi 2010/2011, Avertebrata Air 2011/2012, Mikrobiologi Hasil Perairan 2011/2012 dan 2012/2013), dan Mikroorganisme dan Fermentasi Hasil Perairan 2013/2014. Selain aktif di dalam kampus, penulis juga aktif di kegiatan sosial lingkungan di luar kampus (volunteer of ecofonopoly).
Penulis juga aktif mengikuti lomba karya tulis ilmiah PKM-Penelitian 2010 dan 2012 yang didanai oleh DIKTI. Bulan Juli-Agustus 2012 penulis melaksanakan Praktik Lapangan di PT Istana Cipta Sembada dengan judul “Analisis Bahaya (Hazard Analysis) pada Proses Pembekuan Udang Vannamei (Penaeus vannamei) Peeled and Devined Natural Block di PT Istana Cipta Sembada, Banyuwangi, Jawa Timur”.