KESIMPULAN
Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa promoter keratin ikan flounder Jepang dan heat shock memberikan efektivitas yang sama dan lebih baik dibandingkan dengan promoter medaka β-aktin dan tilapia β-aktin dalam mengendalikan ekspresi GFP pada ikan nila.
SARAN
Guna pengembangan teknologi transgenik pada ikan nila, maka promoter keratin dan heat shock dianjurkan untuk diaplikasikan dalam mengarahkan ekspresi gen target lainnya.
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Lampiran 1. Gamba yang berhhubungan dengan metoodologi pennelitian
1a. Teluur ikan nila yyang ditemppatkan padaa cekungann gel agarossa
A
1b. Seperaangkat alat mikroinjektor. A. Mikroskop B. Neeedle holdeer C. Mikrommanipulator D.Injektor dan magneetic stand
B
1c. Keloompok telur perlakuan (A) dan kelompok elur untuk pengamatan tiap jam
Jarum injjeksi
Blastoodisk
1d. Teluur ikan nila yang diinjeksi pada 1e. Seperaangkat Alaat Pengammatan saatt pembelahaan sel 1 Ekspreesi Gen GFFP. Keteranngan :
A. Mikkroskop B. Kamera Digital C. Lapptop D.Burrner E. Reemote controlller
Jenis
Promoter Ulangan Embrio
PEMG DKH-e DP Σ % Σ % Σ % Keratin 1 30 20 66.67 12 40.00 8 26.67 2 30 13 43.33 8 26.67 5 16.67 Rata-rata 30 16.5 55.00 10 33.33 6.5 21.67 SD - 4.9 16.5 2.8 9.4 2.1 7.1 Heat Shock 1 30 19 63.33 8 26.67 5 16.67 2 30 14 46.67 5 16.67 3 10.00 Rata-rata 30 16.5 55.00 6.5 21.67 4 13.33 SD - 3.5 11.8 2.1 7.1 1.4 4.7 mBA 1 30 11 36.67 21 70.00 15 50.00 2 30 9 30.00 15 50.00 11 36.67 Rata-rata 30 10 33.33 18 60.00 13 43.33 SD - 1.4 4.7 4.2 14.1 2.8 9.4 tiBA 1 30 11 36.67 10 33.33 8 26.67 2 30 7 23.33 10 33.33 7 23.33 Rata-rata 30 9 30.00 10 33.33 7.5 25.00 SD - 2.8 9.4 0.0 0.0 0.7 2.4 Kontrol 1 30 - - 26 86.67 24 80.00 2 30 - - 21 70.00 19 63.33 Rata-rata 30 - - 23.5 78.33 21.5 71.67 SD - - - 3.5 11.8 3.5 11.8
Lampiran 2. Persentase embrio yang mengekspresikan gen GFP, derajat kelangsungan hidup embrio dan derajat penetasan telur ikan nila.
Keterangan:
PEMG = Presentase Embrio yang Mengekspresikan GFP DKH-e = Survival Rate embrio
DP = Hatching Rate
