TERKAIT COVID-19
LABORATORIUM TERKAIT COVID-19
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4*9-%&(-'+ &'&+ @*'8*9-$7-'+ *9*@*'+ 7#'^&+ <-"-&@-'-+ @*'8-"-+
7*$*9-@-%-'+,&)&+,-'+9&'"7#'"-'+$--%+<*7*)8-+,&+:-$&9&%-$+9-<1)-%1)&#@+
3*@*)&7$--'+MTX60NLO+,*'"-'+@*%1,*+419.@*)-$*+M(-&'+C*-^%&1'+
[4MC\+ %*)@-$#7+ -9#)+ 7*)8-Z+ )*-"*'Z+ <-(-'+ (-<&$+ 3-7-&+ ,-'+ 3*)$1'&9+
,&+ 9-<1)-%1)&#@H+ ?*@-@3#-'+ 4MC+ #'%#7+ @*'"(-$&97-'+ -@39&71'+
0/!+ ,-)&+ %-)"*%+ $*7#*'$+ ,-9-@+ 8#@9-(+ .-'"+ $-'"-%+ <-'.-7+ ,-9-@+
(&%#'"-'+@*'&%Z+@*'8-,&7-'+4MC+$*<-"-&+-9-%+,&-"'1$%&7+.-'"+(-',-9H+
/-@#'+ ,&+ $&$&+ 9-&'+ 7-)*'-+ 7*"&-%-'+ ,-9-@+ 3)1$*,#)+ 3*@*)&7$--'+
,-3-%+ @*'&@<#97-'+ 3-8-'-'+ I&)#$+ 3-,-+ 3*%#"-$+ 9-<1)-%1&#@Z+ @-7-+
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@*9&',#'"&+3*%#"-$+9-<1)-%1)&#@Z+9&'"7#'"-'+,-'+-"*'+&%#+$*',&)&+,-)&+
)&$&71+-7&<-%+3-8-'-'+<-(-'+&':*7$&#$+-%-#+%*)8-,&'.-+71'%-@&'-$&+.-'"+
@*'"-7&<-%7-'+(-$&9+31$&%&:a'*"-%&:+3-9$#H
4*)%&@<-'"-'+ .-'"+ <-&7+ ,-'+ (-%&N(-%&+ (-)#$+ ,&<#-%+ #'%#7+
@*',*$-&'+$-)-'-N3)-$-)-'-+,-'+3)1$*,#)+13*)-$&1'-9+,&+>-<1)-%1)&#@+
.-'"+ @*9-7#7-'+ 3*@*)&7$--'+ MTX60NLO+ <*)<-$&$+ 4MCH+ A1,#9+
4*9-%&(-'+ &'&+ <*)&$&+ 3*'"*%-(#-'+ %*'%-'"+ 3)1$*,#)+ <*7*)8-+ ,*'"-'+
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BAB III Materi Inti 1
54 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
2"#$'*+,"()+9-<1)-%1)&#@+%*)7-&%+4*@*)&7$--'+4MC+MTX60NLOH
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=*%*9-(+@*'"&7#%&+@-%*)&+3*$*)%-+@-@3#e LH+ A*9-7#7-'+3*'&9-&-'+)&$&71H
SH+ A*'*)-37-'+3)&'$&3-M##5-T%2#,%(#,)-?,%*("*'-[F>4\H PH+ A*'""#'-7-'+!"#$%&'()-1%2"3'(+[J=M\+,*'"-'+<*'-)+
,-'+-@-'H
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$*$#-&Z
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VH+ A*9-7#7-'+2"#,'=#$"(#,)+$3*$&@*'+MTX60NLOH
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SH+ 4)-7%&7+9-<1)-%1)&#@+@19*7#9*)+.-'"+<-&7 PH+ !"#$%&'()-1%2"3'(+[J=M\
RH+ !9-%+4*9&',#'"+0&)&+[!40\
WH+ 4*'-'"-'-'+%#@3-(-'+,-'+7*^*9-7--'+7*)8-]H+ 4*'"*919--'+9&@<-(
VH+ J&1)*31$&%1)&+$3*$&@*'+MTX60NLO
PEDOMAN 55
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
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<-(-.-+ ,-'+ @*'"*I-9#-$&+ )&$&71+ .-'"+ <*)(#<#'"-'+ ,*'"-'+ i-%+
,-'+ )-^#'+ <&191"&$Z+ ,*'"-'+ @*@3*)%&@<-'"7-'+ 7*9-.-7-'+ ,-)&+
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0*$7)&3$&+ 7*@#'"7&'-'+ 7*8-,&-'+ ,-'+ 71'$*7#*'$&Z+ <*)$-@-+
,*'"-'+ 7*<*)%*)&@--'+ %&'"7-%+ )&$&71+ $*<-&7'.-+ ,&%*'%#7-'+ ,-'+
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56 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
;F$ !4P4L4.$%1.3G434.$:323K0
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RH+ -,-+-"*'+&':*7$&#$+.-'"+<-)#j
WH+ -,-+3*)-9-%-'Z+<-(-'Z+-%-#+)*-"*'+.-'"+<-)#j ]H+ -,-+7*^*9-7-'+7*)8-j
VH+ -,-+3*)#<-(-'+)*"#9-$&H
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J-(-.-+[(%Y%,5\+-,-9-(+$#@<*)Z+7*-,--'Z+-%-#+%&',-7-'+.-'"+
<*)31%*'$&+@*'.*<-<7-'+7*)#$-7-'H
PEDOMAN 57
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
C&$&71+-,-9-(+71@<&'-$&+,-)&+7*@#'"7&'-'+%*)8-,&'.-+<-(-.-+,-'+
7*3-)-(-''.-H
& *UDʈN5LVLNR
=*9-&'+@-%)&7$+)&$&71Z+#'%#7+@*'""-@<-)7-'+7-%*"1)&+)&$&71+8#"-+
GDSDWGLODNXNDQGHQJDQPHQJJXQDNDQJUDʈNULVLNRVHSHUWLGL
<-5-(+&'&H
*DPEDU*UDʈN5LVLNR
=#@<*)e+=-',&-+/-%&1'-9+>-<1)-%1).Z+SULO
0H+ ,423G$%1.3G434.$:323K0
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<*)<*,-+7-)*'-+-,-'.-+3*)<*,--'+:-$&9&%-$Z+3*)-9-%-'+.-'"+-,-Z+
8#@9-(+,-'+71@3*%*'$&+=0AZ+71'%)19+@&%&"-$&+.-'"+-,-Z+-%-#+
7*<&8-7-'+@-'-8*@*'H
58 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
%0K0K$;4P424.$=F$%E4KI3K$-4Q0E4I0E3NH$#0G1KNG1E$V4.M$;43K +F$ D0.I4H3.423
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[-@39&71'\H+!@39&71'+.-'"+%&,-7+,&&'"&'7-'+,-3-%+@*'"(-$&97-'+
(-$&9+3-9$#H
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LH+ 4*'-'"-'-'+ $3*$&@*'Z+ 71'%)19+ 31$&%&:+ -%-#+ -@39&71'+ .-'"+
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RH+ C*-"*'$&-+,-'+<-(-'+(-<&$+3-7-&
WH+ 4*%#"-$+9-<1)-%1)&#@
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4*'^*"-(-'+?1'%-@&'-$&+,&+,-9-@+>-<1)-%1)&#@+A19*7#9*) LH+ 0*$-&'+,-'+1)"-'&$-$&+9-<1)-%1)&#@+.-'"++%*3-%+
SH+ 4)-7%&7+7*)8-+9-<1)-%1)&#@+.-'"+<-&7
PH+ A*'"&7#%&+ =4T+ 7*)8-+ .-'"+ <-&7+ Æ@-'-8*@*'+ 7#-9&%-$+
9-<1)-%1)&#@+.-'"+<-&7
PEDOMAN 59
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
JH+ 61243.$-4Q0E4I0E3NH
4-,-+3)&'$&3'.-Z+-)*-+7*)8-+9-<1)-%1)&#@+#'%#7+3*@*)&7$--'+4MC+
%*)<-"&+@*'8-,&+%&"-+-)*-Z+.-&%#
LH+ )#-'"+3)*3-)-$&+)*-"*'+[3)*3-)-$&+>%$(',->"Z3&5
<*)$&(+[%*7-'-'+#,-)-+31$&%&:\j
SH+ )#-'"+ *7$%)-7$&+ ,-'+ 3*'-@<-(-'+ $-@3*9+ [%*7-'-'+ #,-)-+
'*"-%&:\j+,-'
UXDQJDPSOLʈNDVL=#$(-DPSOLʈNDVLWHNDQDQXGDUDQHJDWLI 0&3&$-(7-'+,*'"-'+
LH+ 3*@<-%-$+)#-'"a%*@<17j SH+ 3*@&$-(-'+$&$&+)#-'"j+,-'
PH+ -9#)+7*)8-+$*-)-(+[-)*-+<*)$&(+[.'$$-*#3(%>"3%3(R%>=."*#3\+
7*+-)*-+71%1)+[:"/:-*#3(%>"3%3(R%>=."*#3\H
F-@<-)+PHS+!9#)+7*)8-+,&+9-<1)-%1)&#@
+++++++++=#@<*)+e+KWWSVZZZHSDJRYVLWHVSURGXFWLRQʈOHV
,1^#@*'%$a*3-Nb-b^N3^)H3,:Z+SUSU
60 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
LH+ C#-'"+3)*3-)-$&+)*-"*'+[3)*3-)-$&+>%$(',->"Z+4MC\
C#-'"+ 3)*3-)-$&+ )*-"*'+ [3)*3-)-$&+ >%$(',- >"Z+ 4MC\+
@*)#3-7-'+ -)*-+ <*)$&(+ .-'"+ <*<-$+ ,-)&+ <*)<-"-&+ -"*'+
<&191"&$+ [C/!Z+ 0/!Z+ $-@3*9Z+ 3)1,#7+ 4MCZ+ 7#9%#)\H+
C#-'"-'+&'&+7(#$#$+#'%#7+@*9-7#7-'e
-H+ 3)*3-)-$&+ ,-'+ %."X+#(+ )*-"*'+ [%."X+#(+ ,&9-7#7-'+ #'%#7+
@*@&'&@-9&$&)+71'%-@&'-$&+,-'+&,''Y'-(:%;\+,-'
<H+ 3)*3-)-$&+,'%*("#3->"Z+#'%#7+4MCH SH+ C#-'"+*7$%)-7$&+,-'+3*'-@<-(-'+$-@3*9
4-,-+)#-'"-'+&'&+,&9-7#7-'+
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%*)$*<#%+(-)#$+%*)^-%-%+,&+.#/-2##KH++4)1")-@+4MC+.-'"+
$#,-(+ ,&-%#)+ ,&+ ,-9-@+ @*$&'+ %&,-7+ <19*(+ ,&#<-(+ 7*^#-9&+
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)#-'"-'+ .-'"+ 3-9&'"+ %*)71'%-@&'-$&+ 19*(+ 3)1,#7+ 4MC+
[-@39&71'\H
PEDOMAN 61
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
5F$ )L4V4$%1.W1M4P4.$D0.I4H3.423$/3$-4Q0E4I0E3NH
<'$(-=5%4("4'
Q3-.-+ 3*'^*"-(-'+ 71'%-@&'-$&+ ,&+ 9-<1)%1)&#@+ -,-9-(+ $*<-"-&+
<*)&7#%>
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SH+ @*'""#'-7-'+ $-)#'"+ %-'"-'+ 5"$=#$%2.'+ ,-'+ "-'%&+ $*$*)&'"+
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OH+ @*9-7#7-'+3*'.&'-)-'+QX+$*%*9-(+,*71'%-@&'-$&+$*9*$-&+&7-+
J=M+$#,-(+%&,-7+,&"#'-7-'j
62 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
LUH+ $*<-"-&+ %-@<-(-'+ 9-)#%-'+ ,*-7%&I-$&+ *'i&@+ $*3*)%&+ +,%$".6P6-/.)*#$".%$'+,-3-%+,&"#'-7-'+#'%#7+@*'"(&9-'"7-'+-@39&71'+
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PEDOMAN 63
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
SH+ @*'"&',&7-$&7-'+ -,-'.-+ 71'%-@&'-$&+ $&9-'"+ [<&-$-'.-+
%*)8-,&+ 7-)*'-+ @-$-9-(+ 3*@&3*%-'\+ 8&7-+ 3-,-+ 71'%)19+ '*"-%&:+
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!"#.#/"*%.- 0%&'()- 1%2"3'(+ [J=M\+ -,-9-(+ 7-<&'*%+ #'%#7+ <*7*)8-+
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-*)1$19H+6%#+<*):#'"$&+#'%#7+@*9&',#'"&+3*%#"-$+9-<Z+9&'"7#'"-'+,-'+
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4)&'$&3+7*)8-+,-)&+J=M+-,-9-(+3*'"*',-9&-'+)&$&71+,*'"-'+$&$%*@+%-%-+
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64 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
D-<*9+PHS+_*'&$+7-<&'*%+,*'"-'+$&$%*@+%-%-+#,-)-+,-'+:#'"$&+3*)9&',#'"-''.-[=#@<*)e+;2T+J&1$-:*%.+A-'#-9+P),+*,&%&1'Z+SUUR\
%&:*8'"- %:86)D -"'CD)'C+'
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[OSHA-@NND\ Ȱ
T4HBP4<-4B<-OTNU+
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Ȱ Ȱ
J=M+?E>!=+66 Ȱ Ȱ Ȱ
J=M+?E>!=+666 Ȱ Ȱ Ȱ
6=T>!DTC Ȱ Ȱ Ȱ
F-@<-)+PHP+D&"-+7-<&'*%+$&$%*@+%-%-+#,-)-+.-'"+#@#@+,&"#'-7-'+,-9-@+
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PEDOMAN 65
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
$ .ODVLʈNDVL%6&
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66 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
LH+ J=M+?*9-$+6
J=M+ ?*9-$+ 6+ @*'-)&7+ #,-)-+ ,-)&+ )#-'"-'+ @-$#7+
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9#-)+.-'"+,&%-)&7+@-$#7+7*+J=M+9-'"$#'"+@*9*5-%&+-)*-+7*)8-+
,-9-@+J=M+%-'3-+ʈOWHUH
F-@<-)+PHR+J=M+?*9-$+6
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J=M+?*9-$+66+@*@<*)&7-'+3*9&',#'"-'+<-"&+3*'""#'-Z+
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PEDOMAN 67
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
J=M+?*9-$+66Z+.-&%#+%&3*+!LZ+!SZ+JLZ+,-'+JSH -H+ J=M+?*9-$+66+D&3*+!L
J=M+?*9-$+66+D&3*+!L+,&"#'-7-'+#'%#7+-"*'+.-'"+
@*@&9&7&+ )&$&71+ )*',-(+ ,-'+ %&,-7+ @*'"-',#'"+ <-(-'+
7&@&-+ <*)-^#'+ -%-#+ )-,&1'#79&,-+ .-'"+ @#,-(+ @*'"#-3H+
4*)^*3-%-'+#,-)-+@-$#7+@&'&@-9+UZPYNUZW+@a$+3-,-+<#7--'+
,*3-'H+ =*9-'8#%'.-Z+ #,-)-+ @-$#7+ 7*+ -)*-+ @*9-9#&+ 7&$&+
#,-)-+[/,"..\+<-"&-'+,*3-'+,-'+<*9-7-'"+3*)@#7--'+7*)8-H+
Q,-)-+,&%-)&7+@-$#7+7*+<-"&-'+<-5-(+7-<&'*%+++%*),-3-%+
2.#;',+ .-'"+ 7*@#,&-'+ @*'"*9#-)7-'+ #,-)-+ @*9-9#&+
39*'#@H+Q,-)-+,&$-)&'"+19*(+2E4!+ʈOWHU+$*<*9#@+,&<#-'"++
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,&<#-'"+ 7*+ 9#-)+ "*,#'"+ @*'""#'-7-'+ $-9#)-'+ #,-)-+
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@-'#-9$a<&1$-:*%.N@-'#-9a^(-3%*)NY\Z+SUSU
68 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
<H+ J=M+?*9-$+66+D&3*+!S
J=M+ ?*9-$+ 66+ D&3*+!S+ @*@&9&7&+ )*$&)7#9-$&+ #,-)-+
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,-'+ PZ+ %1K&'Z+ ,-'+ )-,&1'#79&,-+ 9#.%(".'- %&'"7-%+ )*',-(H+
4*)^*3-%-'+#,-)-+@-$#7+@&'&@-9+UZW+@a$+-%-#+LUU+:%a@&'H+
Q,-)-+@-$#7+,-)&+<#7--'+,*3-'+-7-'+%*)%-)&7+7*+7&$&+#,-)-+
[/,"..\+<-"&-'+,*3-'+,-'+<*9-7-'"+3*)@#7--'+-)*-+7*)8-H+
=*9-'8#%'.-Z+#,-)-+,&%-)&7+19*(+2.#;',+,&+<-"&-'+-%-$+,-)&+
=.'3+>+ ,*'"-'+ %*7-'-'+ '*"-%&:+ 7*+ =.'3+>+ <*)%*7-'-'+
31$&%&:H+ =*<-'.-7+ PUk+ #,-)-+ ,&<#-'"+ 7*9#-)+ ,-'+ VUk+
#,-)-+@-$#7+7*@<-9&+7*+,-9-@+)#-'"-'+-)*-+7*)8-+@*9-9#&+
$-)&'"-'+2E4!+ʈOWHUH+D.3*+J=M+7*9-$+S+%.3*+!S+&'&+-,-+
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PEDOMAN 69
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
^H+ J=M+?*9-$+66+D&3*+JL
J=M+7*9-$+66+%&3*+JL+^1^17+#'%#7+3*7*)8--'+,*'"-'+
-"*'+")#3+)&$&71+%&'"7-%+S+-%-#+PZ+%1K&'Z+,-'+)-,&1'#^9*1%&,*+
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@a$+ -%-#+ LUU+ :%a@&'H+ Q,-)-+ @-$#7+ ,-)&+ <#7--'+ ,*3-'+
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@-$#7+,-)&+<-"&-'+$%$:+%*)%-)&7+7*+7&$&+#,-)-+[/,"..\+<-"&-'+
,*3-'+ ,-'+ <*9-7-'"+ 3*)@#7--'+ -)*-+ 7*)8-Z+ $*,-'"7-'+
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F-@<-)+PHV+J=M+?*9-$+66+D&3*+JL
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70 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
,H+ J=M+?*9-$+66+D&3*+JS
J=M+7*9-$+66+%&3*+JS+^1^17+#'%#7+3*7*)8--'+,*'"-'+
-"*'+,*'"-'+")#3+)&$&71+%&'"7-%+S+,-'+P+%*)"-'%#'"+3-,-++
3*'&9-&-'+ )&$&71Z+ <-(-'+ 7&@&-+ <*)-^#'+ ,-'+ )-,&1'#79&,-+
9#.%(".'+ )*',-(+ $-@3-&+ $*,-'"H+ 4*)^*3-%-'+ -9&)-'+ #,-)-+
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,&<#-'"+ @*@&9&7&+ 5+*("3/+ ,-'+ 39*'#@+ ,*'"-'+ %*7-'-'+
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PEDOMAN 71
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
PH+ J=M+?*9-$+666
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72 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
;*5$N.INK$%1H1E3K244.$*+:*S50?S=
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PEDOMAN 73
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
,-'+ LW+ @*'&%+ %*)$*<#%+ ,&"#'-7-'+ J=M+ #'%#7+ @*@<*)$&(7-'+
7-<&'*%H
P\+ =*<-"&-'+<*$-)+J=M+,&9*'"7-3&+,*'"-'+%.%,>+I&$#-9+,-'+$#-)-H+
4.%,>+J=M+@*@3*)&'"-%7-'+3*%#"-$+9-<1)-%1)&#@+<-(5-+J=M+
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3*%#"-$+ 9-<1)-%1)&#@+ (-)#$+ $*"*)-+ @*'"(*'%&7-'+ 3*7*)8--'+
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R\+ Q'%#7+ @*@-$%&7-'+ J=M+ @-$&(+ <*):#'"$&+ ,*'"-'+ <-&7Z++
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74 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
@*)#$-7+%&)-&+#,-)-+<-(7-'+,-)&+8-)-7+9*<&(+,-)&+P+@*%*)H+
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PEDOMAN 75
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PEDOMAN 77
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PEDOMAN 79
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80 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
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PEDOMAN 83
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PEDOMAN 85
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