SPESIMEN UNTUK DETEKSI PCR COVID-19
PENATALAKSANAAN SPESIMEN UNTUK DETEKSI PCR COVID-19
612KE3L23$*3.MK4I
=3*$&@*'+ -,-9-(+ %-)"*%+ #%-@-+ ,-)&+ 9-<1)-%1)&#@H+ =3*$&@*'+
,&3*)&7$-+ #'%#7+ @*',-3-%7-'+ (-$&9+ -%-#+ $&@3#9-'+ + $#-%#+ 3*'.-7&%H+
Q'%#7+M1I&,NLO++7&%-+(-)#$+@*@-(-@&+$*@#-+3*,1@-'+&'%*)'-$&1'-9+
,-'+'-$&1'-9+,*'"-'+<*'-)+-"-)+@*'"*)%&+8*'&$Z+5-7%#+3*'"-@<&9-'Z+
3*9-<*9-'Z+3*'.&@3-'-'Z+3*'"*@-$-'Z+,-'+3*'"&)&@-'+$3*$&@*'+-"-)+
GDSDWPHQJRQʈUPDVLNDVXV&RYLG
D-(-3-'+ 3*'"-@<&9-'+$3*$&@*'+ @*)#3-7-'+ :-$*+ N3)*-'-9&%&7+
.-'"+-7-'+@*'*'%#7-'+7#-9&%-$+(-$&9+3*@*)&7$--'+9-<1)-%1)&#@+#'%#7+
@*',#7#'"+ *3&,*@&191"&$%+ ,-'+ 79&'&$&+ ,-9-@+ @*@-$%&7-'+ 7-$#$+
M1I&,NLOH++4)1$*,#)+3*'"-@<&9-'+$3*$&@*'+$-'"-%+3*'%&'"+$*(&'""-+
3*%#"-$+3*'"-@<&9+$3*$&@*'+(-)#$+$#,-(+@*'"&7#%&+3*9-%&(-'+,*'"-'+
<-&7+,-'+@*@-(-@&+3)1$*,#)Z+5-7%#Z+-9-%+,-'+<-(-'a@*,&-Z+$*)%-+%-%-+
^-)-+3*'"*',-9&-'+&':*7$&+,*'"-'+<-&7H
=*%*9-(+<*)(-$&9+,&-@<&9+,*'"-'+3)1$*,#)+.-'"+%*3-%Z++$3*$&@*'+
KDUXV GLEHUL LGHQWLʈNDVL VHVXDL GHQJDQ GDWD&#,>+ 3-$&*'+ ,-'+ (-)#$+
,&$&@3-'+3-,-+$#(#+.-'"+%*3-%+7*@#,&-'+,&7*@-$+,*'"-'+<*'-)+3-,-+
$#(#+.-'"+%*3-%+,-'+,&7&)&@+7*+9-<1)-%1)&#@H+4*@1'&%1)-'+[>#3"(#,"3/\+
7#-9&%-$+$3*$&@*'+$-'"-%+3*'%&'"+#'%#7+@*'8-@&'+$3*$&@*'+,&%*)&@-+
,-9-@+71',&$&+<-&7+,-'+%&,-7+)#$-7+$*(&'""-+<&$-+@*@<*)&7-'+(-$&9+
3*@*)&7$--'+.-'"+-7#)-%+,-'+%*)3*)^-.-H BAB IV Materi Inti 2
112 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
!NON4.$)HNH
=*%*9-(+ @*@3*9-8-)&+ + @-%*)&+ &'&Z+ 3*$*)%-+ @-@3#+ @*9-7#7-'+
%-%-+9-7$-'-+=3*$&@*'+#'%#7+,*%*7$&+4MC+M1I&,NLOH+
!NON4.$DPN2N2
=*%*9-(+@*@3*9-8-)&+++@-%*)&+&'&Z+3*$*)%-+@-@3#+++
LH+ @*9-7#7-'+3*'"-@<&9-'+$3*$&@*'+MTX60NLO+#'%#7+
3*@*)&7$--'+,&-"'1$%&7j
SH+ @*9-7#7-'+3*'"*@-$-'+,-'+3*'"&)&@-'+$3*$&@*'+.-'"+
$*$#-&+,*'"-'++M1I&,NLO++7*+9-<1)-%1)&#@+)#8#7-'H PH+ @*9-7#7-'+3*'.&@3-'-'+$3*$&@*'+,&+9-<1)-%1)&#@H
%0K0K$;4P424.
LH+ 4*'"-@<&9-'+=3*$&@*'
SH+ 4*'"*@-$-'+,-'+4*'"&)&@-'+=3*$&@*' PH+ 4*'.&@3-'-'+=3*$&@*'+,&+>-<1)-%1)&#@
)E434.$#4I1E3
%0K0K$;4P424.$<F$%1.M4HQ3G4.$*L123H1.
+F$ 74KIN$%1.M4HQ3G4.$*L123H1.
;-7%#+3*'"-@<&9-'+,-'+"*8-9-+79&'&$+.-'"+@*'.*)%-&+3-$&*'+
$-'"-%+3*'%&'"+#'%#7+@*'*'%#7-'+8*'&$+$3*$&@*'+M1I&,NLO++.-'"+
PEDOMAN 113
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
3*)9#+,&-@<&9H
F-@<-)+RHL+4*)7&)--'+A-$-+6':*7$&+X&)#$+=!C=NM1XNS
#'%#7+A*'*'%#7-'+_*'&$+=3*$&@*'+.-'"+4*)9#+0&-@<&9
=#@<*)e+=*%(#)-@-''(-%.HZ+SUSU
;F$ `1.32$*L123H1.
0-)&+ $3*$&@*'+ %#'""-9Z+ %*)#%-@-+ $3*$&@*'+ .-'"+ <*)-$-9+
,-)&+ $-9#)-'+ 3*)'-3-$-'+ -%-$Z+ (-$&9+ '*"-%&:+ <*9#@+ %*'%#+
@*'"&',&7-$&7-'+ 7*%&-,--'+ &':*7$&H+ T9*(+ 7-)*'-+ &%#Z+ ,&3*)9#7-'+
3*'"#9-'"-'+ 3*'"-@<&9-'+ ,-'+ 3*'"#8&-'+ $3*$&@*'H+ =3*$&@*'+
$-9#)-'+3*)'-3-$-'+<-"&-'+<-5-(+[.#;',-,'$=",%(#,)-(,%*(\+$-'"-%+
,&)*71@*',-$&7-'+ 3-,-+ 3-$&*'+ ,*'"-'+ "*8-9-+ 79&'&$+ .-'"+ 3-)-(+
-%-#+3)1")*$&:H+J&9-+.-'"+,&%*@#7-'+3-%1"*'+9-&'+.-'"+31$&%&:Z+%&,-7+
@*'#%#3+7*@#'"7&'-'+-,-'.-+&':*7$&+M1I&,NLO++7-)*'-+$*8-#(+&'&+
3*)-'+71&':*7$&+<*9#@+,&7*%-(#&+[;2TZSUSU-\H
4*'"-@<&9-'+ $3*$&@*'+ #'%#7+ $#$3*7+ ,&9-7#7-'+ 3-,-+ (-)&+
7*NL+,-'+7*NS+,*'"-'+$*9-'"+5-7%#+rSR+8-@+$*)%-+<&9-++@*@<#)#7Z++
114 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
$*,-'"7-'+ @*9-7#7-'+ 71'%-7+ *)-%+ [7(#$#$+ #'%#7+ 3*%#"-$+
7*$*(-%-'\+ $*"*)-+ ,&9-7#7-'+ 3*@*)&7$--'+ CDN4MC+ $*8-7+ 7-$#$+
,&'.-%-7-'+$*<-"-&+7-$#$+=,#2%2.'DWDXNRQʈUPDVL .HPHQWHULDQ
?*$*(-%-'+C6Z+SUSU\H _*'&$+=3*$&@*'
LH+ =3*$&@*'+$-9#)-'+'-3-$++<-"&-'+-%-$
-H+ Q$-3+'-$1:-)&'"+,-'+1)1:-)&'"
<H+ J&9-$-'+'-$1:-)&'"+-%-#+3%$#=:,)3/'%.-%$=",%(' SH+ =3*$&@*'+$-9#)-'+'-3-$++<-"&-'+<-5-(
=3*$&@*'+$-9#)-'+'-3-$++<-"&-'+<-5-(+,&-@<&9+3-,-+3-$&*'+
,*'"-'+71',&$&+71@39&7-$&+.-'"+9*<&(+<*)-%H -H+ =3#%#@
<H+ A35#(,%*:'%.-%$=",%('
^H+ !,#3*:#%.9'#.%,-.%9%/' ,H+ C,%*:'%.-%$=",%('
*H+ M-&)-'+3-)#
:H+ J&13$&+3-)#
D-<*9+RHL_+*'&$+=3*$&@*'Z+=#(#+3*'.&@3-'-'Z+,-'+4*'"&)&@-'+6,*-9
=#@<*)e+;2TZSUSU
`1.32$2L123H1. ;4P4.$
%1.M4HQ3G4. *NPN$
%1.M3E3H4. %1.V3HL4.4. D1I1E4.M4.
Q$-3+'-$1:-)&'"+
,-'+1)1:-)&'" 0-^)1'+$;%2+
rLS+(-)&e+NVU1M+
[5,)-"*'Q
rW+(-)&e+NVU1M+
[5,)-"*'Q
PEDOMAN 115
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
J)1'^(1-9I*19-)+
>-I-"* ?1'%-&'*)+
=%*)&9 SNY1M ȸMDP 1M
rRY+8-@e+NVU1M+
[5,)-"*'Q
rRY+8-@e+NVU1M+
[5,)-"*'Q rSR+8-@e+NVU1M+
[5,)-"*'Q rW+(-)&e+NVU1M+
[5,)-"*'Q
5F$ %1.W1M4P4.$/4.$%1.M1./4G34.$"._1K23$K1I3K4$%1.M4HQ3G4.$
*L123H1.
=*<*9#@+ 7*"&-%-'+ 3*'"-@<&9-'+ $3*$&@*'+ ,&9-7$-'-7-'Z+
3*)$1'*9+ (-)#$+ @*@3*)(-%&7-'+ +3"9',$%.- =,'*%+("#3- -%-#+
7*5-$3-,--'+ #'&I*)$-9+ #'%#7+ @*'^*"-(+ %*)8-,&'.-+ 3*'#9-)-'+
3*'.-7&%Z+ <-&7+ ,-)&+ 3-$&*'+ 7*+ 3-)-@*,&$+ @-#3#'+ 9&'"7#'"-'+
$*7&%-)H+2-9+%*)$*<#%+@*9&3#%&+++
LH+ $*9-9#+@*'^#^&+%-'"-'+,*'"-'+@*'""#'-7-'+$-<#'a,&$&':*7%-'+
$'2'.+>+,-'+$'$+5%:+%&',-7-'+,-'+
SH+ @*'""#'-7-'+!40H
116 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
6F$ +G4I$/4.$;4P4.$21EI4$%E021/NE$%1.M4HQ3G4.$*L123H1.
LH+ !9-%+,-'+J-(-'+4*'"-@<&9-'+=3*$&@*' -H+ G1)@#9&)+4*'.*9&,&7-'+E3&,*@&191"&
4*'"&)&@-'+$3*$&@*'+7*+9-<1)-%1)&#@+(-)#$+,&$*)%-&+
,*'"-'+:1)@#9&)+3*'.*9&,&7-'H+E3&,*@&191"&+%*)9-@3&)+
$*$#-&+,*'"-'+5-7%#+3*'"-@<&9-'+$3*$&@*'H
<H+ =3*$&@*'+=-9#)-'+4*)'-3-$-'+[<'$=",%(#,)-C,%*(Q L\+ I",+$-C,%3$=#,(-H'5"%-[XDA\+[LNS+@9\
J*<*)-3-+ @*)*7+ 71@*)$&-9+ + .-'"+ $#,-(+ $&-3+ 3-7-&+
,-3-%+ ,&"#'-7-'+ -%-#+ ,*'"-'+ @*'^-@3#)+ <*<*)-3-+
<-(-'+ [2-'7$+ J==j+ -'%&:#'"-9+ ,-'+ -'%&<&1%&7+ ,*'"-'+
71@31$&$&+%*)%*'%#\+#'%#7+,&$-%#7-'+,-9-@+$-%#+5-,-(+
$%*)&9H+ 2&',-)&+ 3*'""#'--'+ + XDA+ .-'"+ @*'"-',#'"+
<-(-'+@*'"&'-7%&:-$&+I&)#$+-%-#+9&$&$+<#::*)H S\+ O.#*K'5-$;%2a5%*,#3-$;%2
P\+ C#3/+'-$=%('.
R\+ ?1'%-&'*)+$%*)&9+#'%#7+$3#%#@
3DUDʈOP ]\+ 49-$%&7+79&3
V\+ A-)7*)+-%-#+9-<*9+60
PEDOMAN 117
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
F-@<-)+RHS+!9-%+,-'+J-(-'+4*'"-@<&9-'+=3*$&@*'
=#@<*)e+J-,-'+>&%<-'"7*$Z+SUSU
^H+ =3*$&@*'+0-)-(a$*)#@
L\+ D1)'&b#*%
S\+ 0=+"(-5"$=#$%2.'+-%-#+W+@9+$)$('>-9%*+(%"3', P\+ U"3/-3''5.'+[8&7-+,&3*)9#7-'\
R\+ ?-3-$+-971(19+VUk W\+ ?-3-$+7*)&'"
]\+ X&-9+ LZY+ @9+ -%-#+ %-<#'"+ %#%#3+ #9&%+ [5-,-(+ $3*$&@*'+
$*)#@\
V\+ A-)7*)+-%-#+9-<*9+60
118 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
,H+ J-(-'+4*'"*3-7-'a4*'"&)&@-'+=3*$&@*' L\+ B*'-=%*K+,-'+1#.5-!#Z
- B*'- =%*K+ ,-'+ 1#.5- !#Z+ ,&3*)9#7-'+ <&9-+ %*@3-%+
3*'"-@<&9-'+ $3*$&@*'+ %*)3&$-(+ ,-)&+ 9-<1)-%1)&#@H+
4-$%&7-'+"*'-=%*K+.-'"+,&"#'-7-'+<*'-)N<*'-)+<*7#H S\+ >-<*9+!9-@-%
P\+ 4*)*7-%+a+>-7<-'
SH+ 4)1$*,#)+4*'"-@<&9-'+=3*$&@*' -H+ =3*$&@*'+$-9#)-'+3*)'-3-$-'+-%-$
L\+ =&-37-'+*,)#(+2'+.-'"+<*)&$&+LZW+@9+XDA+[<*)&$&+2-'7$+
J==+w+-'%&:#'"-9+w+-'%&<&1%&7\Z+,-3-%+8#"-+@*'""#'-7-'+
XDA+71@*)$&-9++.-'"+$&-3+3-7-&H+J*)&9-<*9+.-'"+<*)&$&+
'-@-+ 3-$&*'+ ,-'+ 71,*+ + '1@1)+ + $3*$&@*'H+ >-<*9+ &'&+
(-)#$+ $*$#-&+ ,*'"-'+ &,*'%&%-$+ 3-$&*'+ 3-,-+ :1)@#9&)+
3*'.*9&,&7-'+*3&,*@&191"&+3-$&*'H+
S\+ _&7-+ 9-<*9+ <*)'1@1)+ %&,-7+ %*)$*,&-Z+ + 3*'-@--'+ <&$-+
@*'""#'-7-'+ @-)7*)+ 3*)@-'*'+ 3-,-+ <-"&-'+ 3#%&(+
,&',&'"+ *,)#(+2'H+ _-'"-'+ "#'-7-'+ XDA+ [2-'7$\+ <&9-+
%*9-(+<*)#<-(+5-)'-+@*'8-,&+7#'&'"H
P\+ F#'-7-'+ $;%2+ .-'"+ %*)<#-%+ ,-)&+ <-(-'+ ,-^)1'a)-.1'a
$&'%*'%&7+ ʈEHU+ $%*)&9+ ,*'"-'+ %-'"7-&+ 39-$%&7+ -%-#+ 8*'&$+
O.#*K'5-0;%2H+_-'"-'+@*'""#'-7-'+$;%2+7-3-$+-%-#+
$;%2+ .-'"+ @*'"-',#'"+ 1%.*"+>- 4./"3%('+ -%-#+ $;%2+
7-3-$+,*'"-'+%-'"7-&+7-.#+7-)*'-+@#'"7&'+@*'"-',#'"+
$#<$%-'$&+ .-'"+ ,-3-%+ @*'"&'-7%&I-$&+ I&)#$+ ,-'+ ,-3-%+
PEDOMAN 119
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
@*'"(-@<-%+3)1$*$+3*@*)&7$--'+$*^-)-+@19*7#9*)H -\+ Q$-3+'-$1:-)&'"
LH+ 4-$%&7-'+%&,-7+-,-+1<$%)#7$&+[(-@<-%-'+3-,-+
9#<-'"+(&,#'"\H
SH+ A-$#77-'+ ʉRFNHG VZDE+ @*9-9#&+ 9#<-'"+
(&,#'"+ $*8-8-)+ ,*'"-'+ 9-'"&%N9-'"&%+ @#9#%+
[%&,-7+ 7*+ -%-$\+ $-@3-&+ %*)8-,&+ )*$&$%*'$&+ -%-#+
8-)-7+.-'"+$*%-)-+,*'"-'+8-)-7+,-)&+%*9&'"-+7*+
9#<-'"+(&,#'"+3-$&*'+.-'"+@*'"&',&7-$&7-'+
71'%-7+,*'"-'+'-$1:-)&'"H+
PH+ M-)-+ #$-3+ (-)#$+ @*'^-3-&+ 7*,-9-@-'+ .-'"+
$-@-+ ,*'"-'+ 8-)-7+ ,-)&+ 9#<-'"+ (&,#'"+ 7*+
9#<-'"+ 9#-)+ %*9&'"-+ "1$17+ ,-'+ 3#%-)+ ,*'"-'+
9*@<#%H+ J&-)7-'+ $;%2+ ,&+ %*@3-%+ $*9-@-+
<*<*)-3-+,*%&7+#'%#7+@*'.*)-3+$*7)*$&H+D-)&7+
$;%2+ 3*)9-(-'+ $--%+ @*@#%-)'.-H+ =3*$&@*'+
#$-3+'-$1:-)&'"+^#7#3+,&-@<&9+,-)&+$-9-(+$-%#+
'1$%)&9+$-8-H+
x+ /-@#'Z+ 8&7-+ ,&)-$-7-'+ 3*)9#Z+ ,-3-%+
@*'"-@<&9+ ,-)&+ 7*,#-+ '1$%)&9+ @*'""#'-7-'+
$;%2+ .-'"+ $-@-Z+ %*%-3&+ %&,-7+ 3*)9#+
@*'"#@3#97-'+$3*$&@*'+,-)&+7*,#-+$&$&+8&7-+
$;%2+ %*9-(+ 8*'#(+ ,*'"-'+ ^-&)-'+ ,-)&+ 719*7$&+
3*)%-@-H+_&7-+-,-+$#@<-%-'+,-)&+$-%#+9#<-'"+
(&,#'"Z+ "#'-7-'+ $;%2+ .-'"+ $-@-+ #'%#7+
@*',-3-%7-'+ $3*$&@*'+ ,-)&+ 9#<-'"+ (&,#'"+
120 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
9-&''.-H
F-@<-)+RHP+>17-$&+4*'"-@<&9-'+/-$1:-)&'"
=#@<*)e+M0MZ+SUSU
RH+ ?*@#,&-'+@-$#77-'+$;%2+7*+,-9-@-*,)#(+2'+
<*)&$&+XDA+$*$*"*)-+@#'"7&'H
WH+ !'"7-%+$*,&7&%+$;%2+,-)&+,-$-)+*,)#(+2'+9-9#+
3-%-(7-'+ %-'"7-&+ 39-$%&7+ ,&+ ,-*)-(+
@#9#%-*,)#(+2'+-"-)-*,)#(+2'-,-3-%+,&%#%#3+,*'"-'+
)-3-%H
F-@<-)+R+R+A*@-$#7-'+0;%2+7*+,-9-@+XDA
=#@<*)e+J-,-'+>&%<-'"7*$Z+SUSU
PEDOMAN 121
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
<\+ Q$-3+T)1:-)&'"
LH+ F#'-7-'+(#3/+'-$=%('.+8&7-+,&3*)9#7-'+#'%#7+
@*'-(-'+ 9&,-(+ 7*%&7-+ -7-'+ @*'"-@<&9+ #$-3+
1)1:-)&'"H
SH+ A-$#77-'+$5-<+7*+:-)&'"+31$%*)&1)+,-'+-)*-+
%1'$&9H+ F1$177-'+ -3#$-'+ 3-,-+ 7*,#-+ 3&9-)+
%1'$&9+ ,-'+ 1)1:-)&'"+ 31$%*)&1)+ ,-'+ (&',-)&+
@*'.*'%#(+9&,-(Z+"&"&Z+,-'+"#$&H
F-@<-)+RHW+>17-$&+4*'"-@<&9-'+1)1:-)&'"
=#@<*)e+A#))-.'(-%.HZ+SULR
PH+ ?*@#,&-'+ @-$#77-'+ $;%2+ 7*+ ,-9-@+
*,)#(+2'+<*)&$&+XDA+.-'"+$-@-+,*'"-'+$;%2+
'-$1:-)&'"+$*$*"*)-+@#'"7&'H
RH+ !'"7-%+$*,&7&%+$;%2+,-)&+,-$-)+*,)#(+2Z+9-9#+
3-%-(7-'+ %-'"7-&+ 39-$%&7+ ,&+ ,-*)-(+ @#9#%+
*,)#(+2'+-"-)+*,)#(+2'-,-3-%+,&%#%#3+,*'"-'+
)-3-%H
122 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
F-@<-)+RH]+A*@-$#7-'+0;%2+7*+,-9-@+XDA .-'"+=-@-+,*'"-'+=5-<1)1:-)&'"
=#@<*)e+J-,-'+>&%<-'"7*$Z+SUSU
WH+ 4-$%&7-'+9-<*9+71,*+$3*$&@*'+$*$#-&+,*'"-'+
71,*+ .-'"+ -,-+ ,&+ :1)@#9&)+ 3*'.*9&,&7-'+
*3&,*@&191"&H
]H+ 1),#(+2' NHPXGLDQ GLOLOLW SDUDʈOP GDQ
@-$#77-'+ 7*+ ,-9-@+ 39-$%&7+ 79&3a*,)#2#ZH+
_&7-+ -,-+ 9*<&(+ ,-)&+ L+ 3-$&*'Z+ + 39-$%&7+ 79&3+
,&<*,-7-'a%*)3&$-(+ #'%#7+ @*'"(&',-)&+
71'%-@&'-$&+$&9-'"H
F-@<-)+RHV+4*'"*@-$-'+=3*$&@*'
=#@<*)e+J-,-'+>&%<-'"7*$Z+SUSU
PEDOMAN 123
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
<H+ =3*$&@*'+$-9#)-'+3*)'-3-$-'+<-5-(
=3#%#@
4-$&*'+,&<*)&+%-(#+++3*)<*,--'+$3*$&@*'+$3#%#@+,-'+$-9&I-+
[-&)+ 9&#)\H+ 4-$&*'+ <*)7#@#)+ %*)9*<&(+ ,-(#9#+ ,*'"-'+ -&)+
7*@#,&-'+3-$&*'+,&@&'%-+@*'"*9#-)7-'+,-(-7'.-+,*'"-'+
^-)-+ <-%#7+ .-'"+ ,-9-@H+ =3#%#@+ ,&%-@3#'"+ 3-,-+ 5-,-(+
$%*)&9+.-'"+-'%&+<1^1)H+4*'"-@<&9-'+$-@3*9+$3#%#@+,*'"-'+
^-)-+&',#7$&+,-3-%+@*'&@<#97-'+)&$&71+&':*7$&+%-@<-(-'+
<-"&+3*%#"-$+7*$*(-%-'H
^H+
=3*$&@*'+9-&''.-=*)#@
=*3-$-'"+$-@3*9+$*)#@+[-7#%+,-'+71'I-9*$*'\+,&3*)9#7-'+
XQWXN NRQʈUPDVL 6HUXP DZDO GLNXPSXONDQ GL PLQJJX
3*)%-@-+ 3*'.-7&%+ ,-'+ $*)#@+ .-'"+ 7*,#-+ &,*-9'.-+
,&7#@3#97-'+S`P++@&'""#+7*@#,&-'H+J&9-+$*)#@+%#'""-9Z+
3*'"-@<&9-'+ (-)#$+ ,&9-7#7-'+ $*%&,-7'.-+ LR+ (-)&+ $*%*9-(+
1'$*%+"*8-9-+#'%#7+3*'*'%#-'+7*@#'"7&'-'+7-$#$H+
&F$ 60KNH1.I423$*L123H1.$/4.$64I4$%4231.
4*'^-%-%-'+3*'"-@<&9-'+$3*$&@*'+3-$&*'+,&)*71@*',-$&7-'+
-,-+ ,&+ .#/2##KH+ =*@#-+ 3-$&*'+ + ,&-@<&9+ $3*$&@*''.-+ %*)^-%-%+
,*'"-'+ <-&7+ ,-'+ )-3&Z+ $*3*)%&+ ,-%-+ '-@-+ 3-$&*'Z+ '1@1)+ )*7-@+
@*,&7Z+%-'""-9+3*'"-@<&9-'+$3*$&@*'Z+8*'&$+$3*$&@*'+.-'"+,-3-%+
,&-@<&9Z+ 71,*+ 3*'-@--'+ &,*'%&%-$+ 3-$&*'Z+ %-'""-9+ 3*'"&)&@-'+
$3*$&@*'+7*+9-<1)-%1)&#@Z+,-'+(-$&9+3*@*)&7$--'H
124 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
9F$ %1.V3HL4.4.$21H1.I4E4$2L123H1.$21Q1GNH$L1.M3E3H4.$
2L123H1.
=3*$&@*'+,-)&+$#$3*7+M1I&,+LO++(-)#$+,&$&@3-'+,-'+,&7&)&@+
3-,-+ $#(#+ .-'"+ $*$#-&+ [9&(-%+D-<*9+ PHL\H+ =3*$&@*'+ (-)#$+ %&<-+ ,&+
9-<1)-%1)&#@+ $*"*)-+ $*%*9-(+ 3*'"-@<&9-'H+ J&9-+ 7-)*'-+ -9-$-'+++
%*)%*'%#+3*'"&)&@-'+(-)#$+,&%#',-Z+$3*$&@*'+(-)#$+,&$&@3-'+3-,-+
$#(#+ + SNNY1MH+ D*@3-%+ 3*'.&@3-'-'+ $3*$&@*'+ (-)#$+ %*)$*',&)&Z+
%*)3&$-(+,-)&+3*'.&@3-'-'+)*-"*'$&-H+J&9-+@*'""#'-7-'+9*@-)&+*$+
,1@*$%&7Z+(&',-)&+@*'.&@3-'+$3*$&@*'Z+<-&7+,&+3&'%#Z+)-7+*:"..',+
@-#3#'+&,''Y',+#'%#7+@*'^*"-(+7*)#$-7-'+$3*$&@*'H
F-@<-)+RHY+D*@3-%+4*'.&@3-'-'+=3*$&@*'+=*@*'%-)-+=*<*9#@+4*'"&)&@-'
=#@<*)e+J-,-'+>&%<-'"7*$Z+SULL
PEDOMAN 125
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
%0K0K$;4P424.$=F$%1.M1H424.$/4.$%1.M3E3H4.$*L123H1.
!H+%E3.23L$:4.I43$/3.M3.$/4G4H$%1.M1H424.$/4.$%1.M3E3H4.$
*L123H1.
=3*$&@*'+#'%#7+3*@*)&7$--'+4MC+=!C=NM1XNS+@*)#3-7-'+
@-%*)&-9+ <&191"&+ .-'"+ $-'"-%+ 3*7-+ %*)(-,-3+ 3*)#<-(-'+ $#(#H+
=%-<&9&%-$+ I&)#$+ =!C=NMTXNS+ $-'"-%+ ,&3*'"-)#(&+ 19*(+ $#(#+
3*'.&@3-'-'+ ,-'+ $#(#+ $*9-@-+ %)-'$31)%-$&H+ A-7&'+ %&'""&+ $#(#+
3*'.&@3-'-'+,-'+%)-'$31)%-$&Z+$%-<&9&%-$+I&)#$+=!C=NM1XNS+@-7&'+
@*'#)#'H+4-,-+$#(#+R1M+I&)#$+=!C=NM1XNS+<&$-+<*)%-(-'+(&'""-+
LS+(-)&H
D-<*9+RHS+=%-<&9&%-$+X&)#$+=!C=NM1XNS+3-,-+J*<*)-3-+=#(#
=#@<*)e+M(&'+'(-%.HZ+SUSU
T9*(+ 7-)*'-+ &%#,-9-@+ + 3*'"*@-$-'+ ,-'+ '+ 3*'"&)&@-'+
$3*$&@*'+ ,&3*)9#7-'+ @-'-8*@*'+ )-'%-&+ ,&'"&'+ #'%#7+ @*@-$%&7-'+
7#-9&%-$+ $3*$&@*'+ %*%-3+ %*)8-"-+ $-@3-&+ 3-,-+ %-(-3+ 3*@*)&7$--'H+
0HQXUXWGHʈQLVLGDUL:+2UDQWDLGLQJLQDGDODKVXDWXVLVWHP\DQJ
<*)3*)-'+ @*@3*)%-(-'7-'+ 3*'.&@3-'-'+ ,-'+ %)-'$31)%-$&+ <-(-'+
<&191"&a$-@3*9a$3*$&@*'+ 3-,-+ 71',&$&+ ,-'+ $#(#+ 13%&@-9+ .-'"+
,&)*71@*',-$&7-'+[;2TZ+SUSU^\H
126 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
A-'-8*@*'+)-'%-&+,&'"&'+<*)%#8#-'+#'%#7+@*@-$%&7-'+<-(5-+
$3*$&@*'+.-'"+,&-@<&9+,&+:-$.-'7*$+%*)8-"-+@#%#'.-+$-@3-&+,*'"-'+
,&9-7#7-'+3*@*)&7$--'+,&+9-<1)-%1)&#@+)#8#7-'+$*(&'""-+,&,-3-%7-'+
(-$&9+ 3*@*)&7$--'+ .-'"+ -7#)-%+ ,-'+ %*)3*)^-.-H+ 4*)#<-(-'+ $#(#+
,-)&+ <-%-$+ .-'"+ ,&)*71@*',-$&7-'+ $*9-@-+ 3)1$*$+ 3*'.&@3-'-'Z+
%)-'$31)%-$&Z+$-@3-&++,*'"-'+-'-9&$&$+,-3-%+@*@*'"-)#(&++I&-<&9&%-$a
@#%#+ $3*$&@*'+ $*(&'""-+ ,-3-%+ @*'.*<-<7-'+ (-$&9+ .-'"+ %&,-7+
-7#)-%H+
A*'#)#%+ C1,)&"#*+ ,-'+ /1%%*<11@+ [SUSU\+ ,-9-@+ <#7#+ .-'"+
<*)8#,#9+C:'-1#.5-1:%"3-%35-"($-T#/"$("*$Z+-,-+*@3-%+*9*@*'+#%-@-+
)-'%-&+,&'"&'Z+.-&%#++$*<-"-&+<*)&7#%e
LH+ $&$%*@+3*',&'"&'+[*##."3/-$)$('>$\e+$#-%#+$&$%*@+-%-#+:-$&9&%-$+
.-'"+ @*'.*,&-7-'+ $#(#+ .-'"+ %*3-%+ #'%#7+ 3*'"19-(-'Z+
3*'.&@3-'-'+ ,-'+ 3*'"&)&@-'+ 3)1,#7+ ,-9-@+ (-9+ &'&+ -,-9-(+
@-%*)&-9+<&191"&j
SH+ $&$%*@+ 3*'.&@3-'-'+ <*)3*',&'"&'- W*#.5- $(#,%/'\+ e+ :-$&9&%-$+
3*'.&@3-'-'+ <*)3*',&'"&'Z+ <-&7+ .-'"+ + ,&"#'-7-'+ $*@*'%-)-+
$*<*9#@+ 3*'"&)&@-'+ 3)1,#7+ @-#3#'+ 3*'.&@3-'-'+ .-'"+
$&:-%'.-+9*<&(+9-@-+[.#3/-(',>\+@&$-9'.-+$*3*)%&+<&1)*31$&%1)&j PH+ $&$%*@+%)-'$31)%-$&+<*)3*',&'"&'-W*#.5-(,%3$=#,(Q-e+$&$%*@+.-'"+
@-@3#+ @*'8-"-+ 7*$%-<&9-'+ $#(#Z+ 7*9*@<-3-'+ + 13%&@-9+ ,-'+
&'%*")&%-$+3)1,#7+$*9-@-+3)1$*$+3*'"&)&@-'j
RH+
3*@)1$*$-'+,-'+3*'"&)&@-'+[*#.5-=,#*'$$"3/-%35-5"$(,"2+("#3QJ-%*)$*,&-'.-+:-$&9&%-$+#'%#7+3*@)1$*$-'+,-'+3*'"&)&@-'+3)1,#7+H
PEDOMAN 127
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
0-9-@+ + 3*'"*@-$-'+ ,-'+ 3*'"&)&@-'+ $3*$&@*'+ ,&3*)9#7-'+
<*<*)-3-+3*)-9-%-'+.-'"+,-3-%+@*@*9&(-)-+,-'+@*@-'%-#+71',&$&+
3*'.&@3-'-'+,-'+3*'"&)&@-'+$3*$&@*'H+4*)-9-%-'+&'&+,&7-%*"1)&7-'+
@*'8-,&+%&"-+7*91@317Z+.-&%#+%*("9'-*#.5-*:%"3Z+=%$$"9'-*#.5-*:%"3+
,-'+('>=',%(+,'->#3"(#,"3/-(##.$H
-H+ 4*("9'- *#.5- *:%"3- -,-9-(+ 3*)-9-%-'+ .-'"+ ,-3-%+ @*'"(-$&97-'+
$#(#+ ,&'"&'+ #'%#7+ 3*'.&@3-'-'+ $3*$&@*'Z+ @&$-9'.-+ &,''Y',-,-'+ ,'&,"/',%(#,H+ J*<*)-3-+ (-9+ .-'"+ (-)#$+ ,&3*)%&@<-'"7-'+
,-9-@+ @*@&9&(+ &,''Y',+ -%-#+ ,'&,"/',%(#,L+ -'%-)-+ 9-&'+ -,-9-(+
7-3-$&%-$Z+ 8*'&$+ 9*@-)&+ 3*',&'"&'+ [%+(#R>%3+%.- 5'&,#$(\Z+ $*)%-+
-,-'.-+ $&$%*@+ 3*@1'&%1)-'+ [>#3"(#,"3/\+ $#(#H+ O,''Y',+ -%-#+
,'&,"/',%(#,+ 8*'&$+ %+(#65'&,#$("3/+ %&,-7+ ,&-'8#)7-'+ #'%#7+
3*'.&@3-'-'+ $3*$&@*'+ 7-)*'-+ -,-'.-+ $&$%*@+ 3*@-'-$+ .-'"+
-7-'+ <*7*)8-+ $*^-)-+ 1%1@-%&$+ @*@<*)$&(7-'+ <#'"-+ *$+ .-'"+
%*)<*'%#7+$*(&'""-+<*)&$&71+@*'&@<#97-'+7*)#$-7-'+$3*$&@*'H
<H+ ?%$$"9'-*#.5-*:%"3+-,-9-(+3*)-9-%-'+.-'"+,-3-%+@*'8-"-+$#(#+
,&'"&'+ ,-9-@+ 5-7%#+ .-'"+ %*)<-%-$+ ,-'+ %&,-7+ @*@<#%#(7-'+
$#@<*)+ *'*)"&+ #'%#7+ 3*@-':--%-''.-Z+ ^1'%1('.-+ *##.-
2#ZL-"*'-=%*KL-5,)-"*'H+B*'-=%*K+.-'"+-7-'+,&"#'-7-'+#'%#7+3)1$*$+
%)-'$31)%-$&+$3*$&@*'+(-)#$+<*'-)N<*'-)+,-9-@+7*-,--'+<*7#H+
_#@9-(+"*'-=%*K+.-'"+,&"#'-7-'+(-)#$+$*$#-&+,*'"-'+$3*$&@*'+
.-'"+,&7&)&@+,-'+#7#)-'+*##.-2#Z-.-'"+,&"#'-7-'H+D,)-"*'-,-3-%+
,&"#'-7-'+ #'%#7+ 3*'"&)&@-'+ $3*$&@*'+ -3-<&9-+ ,&3*)7&)-7-'+
$3*$&@*'+<-)#+-7-'+$-@3-&+,-9-@+5-7%#+9*<&(+,-)&+P+(-)&H
^H+ C'>=',%(+,'->#3"(#,"3/-(##.$+-,-9-(+3*)-9-%-'+#'%#7+3*@-'%-#-'+
$#(#Z+<-&7+$*9-@-+3*'.&@3-'-'+@-#3#'+3*'"&)&@-'+$3*$&@*Z'+
@&$-9'.-+ %*)@1@*%*)+ ,-'+ 5%(%- .#//',H+ J*),-$-)7-'+ 3*)&1,*+
128 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
3*@-'%-#-'+ $#(#'.-Z+ ,&7*'-9+ ,#-+ @-^-@+ -9-%+ 3*@-'%-#-'+
$#(#Z+.-&%#+-9-%+3*@-'%-#-'+$#(#+$*$--%+,-'+-9-%+3*@-'%-#-'+
$#(#+<*)7*9-'8#%-'H+!9-%+3*@-'%-#-'+$#(#+$*$--%+(-'.-+,-3-%+
@*'"#7#)+ $#(#+ 3-,-+ $#-%#+ $--%+ %*)%*'%#Z+ + @&$-9'.-+ $#(#+ $--%+
$3*$&@*'+,&7&)&@+-%-#+$--%+$3*$&@*'+,&%*)&@-H+=*@*'%-)-+&%#Z++
-9-%+ 3*@-'%-#-'+ $#(#+ <*)7*9-'8#%-'+ ,-3-%+ @*'^-%-%+ )&5-.-%+
3*)#<-(-'+$#(#+$*9-@-+3*)8-9-'-'+$3*$&@*'+@#9-&++3*'"&)&@-'+
$-@3-&+3*'*)&@--'+$3*$&@*'+,&+%#8#-'+[D15'$*',Z+SULP\H
F-@<-)+RHO+!9-%+4*@-'%-#+=#(#+=*$--%+[!\+,-'+J*)7*9-'8#%-'+[J\
=#@<*)e+J-,-'+>&%<-'"7*$Z+SULU
;F$%1.M1H424.$/4.$%1.M3E3H4.$*L123H1.
=3*$&@*'+ M1I&,NLO+ + @*)#3-7-'+ s=#<$%-'$&+ J&191"&$Z+
?-%*"1)&+JtZ+,-'+(-)#$+,&7*@-$+<*),-$-)7-'+%-%-+9-7$-'-+Q/PPVP+
7*%&7-+ -7-'+ ,&-'"7#%a,&%)-'$31)%-$&7-'+ ,*'"-'+ %#8#-'+ ,&-"'1$%&7+
-%-#+ &'I*$%&"-$&H+ ?-%*"1)&+ &'&+ @*9&',#'"&+ $3*$&@*'Z+ 3*%#"-$+
9-<1)-%1)&#@Z+,-'+8#"-+-"*'+3*'"&)&@-'+$3*$&@*'H+=*@#-+$3*$&@*'+
(-)#$+,&7*@-$+#'%#7+@*'^*"-(+7*)#$-7-'+,-'+%#@3-(-'H+=&$%*@+
.-'"+ ,&"#'-7-'+ -,-9-(+ ,*'"-'+ @*'""#'-7-'+ %&"-+ 9-3&$+ [C:,''- T%)',-?%*K%/"3/\+$*$#-&+,*'"-'+3*,1@-'+;2T+,-'+B3(',3%("#3%.-4",-C,%3$=#,(-4$$#*"%("#3-[6!D!\[;2TZ+SUSU<\H
PEDOMAN 129
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
F-@<-)+RHLU+M1'%1(+4*'"*@-$-'+D&"-+>-3&$
=#@<*)e+U@N6M+"5%3*'-#3-,'/+.%("#3$-&#,-(:'-(,%3$=#,(-#&-"3&'*("#+$-$+2$(%3*'$-EF786EFEF
=&$%*@+ 3*'"*@-$-'+ %&"-+ 9-3&$+ %*),&)&+ -%-$+ + %&"-+ 9-3&$-'Z+
.-&%#+ 9-3&$+ 3)&@*)Z+ $*7#',*)Z+ ,-'+ %*)$&*)H+ >-3&$-'+ 3)&@*)+ + -,-9-(++
%-<#'"+7*,-3+-&)+%*@3-%+@*'-@3#'"+$3*$&@*'H+>-3&$-'+$*7#',*)+
<*)#3-+39-$%&7+Y"=.#*Ka*,)#2#Za2"#2#((.'+.-'"+,&%-@<-(7-'+<-(-'+
-<$1)<*'Z+ $*3*)%&+ ("$$+'- (#;'.H+ >-3&$-'+ 7*%&"-+ -,-9-(+ *##.- 2#Za
$='$">'3-*%,,"',+$*3*)%&+$(,)#&#%>+-%-#+Q/+2#ZH+
=--%+ 3*'"&)&@-'Z+ $3*$&@*'+ ,&%*@3-%7-'+ ,&+ ,-9-@+ *##.- 2#Z-,*'"-'+ 71',&$&+ $#(#+ SNYvMH+ 4*'"&)&@-'+ $3*$&@*'Z+ <-&7+ 7-$#$+
$#$3*7+ M1I&,NLO+ + @-#3#'+ 71'%-7+ *)-%+ ,&9-7#7-'+ 19*(+ 3*%#"-$+
0&'-$+ ?*$*(-%-'+ ,*'"-'+ @*'.*)%-7-'+ :1)@#9&)+ 3*'.*9&,&7-'+
*3&,*@&191"&H+ 4*'"&)&@-'+ $3*$&@*'+ ,&%#8#7-'+ 7*+ 9-<1)-%1)&#@+
3*@*)&7$-+.-'"+%*9-(+@*@*'#(&+3*)$.-)-%-'+.-'"+,&%*%-37-'+19*(+
A*'%*)&+?*$*(-%-'+-%-#+3*8-<-%+.-'"+,&%#'8#7H+
130 PEDOMANPEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
J-(-'N<-(-'+.-'"+,&<#%#(7-'+#'%#7+3*'"*@-$-'+,-'+3*'"&)&@-'+
$3*$&@*'+-,-9-(++
-H+ 39-$%&7+79&3a*,)#2#Za2"#2#((.'\
<H+ <-(-'+-<$1)<*'%e+("$$+'-(#;'.\
^H+ "*'-=%*K$\-,H+ "3$+.%(#,\
*H+ 9-<*9+-9-@-%+,-'+9-<*9+Q/PPVPj :H+ 9-7<-'a3*)*7-%j
"H+ 39-$%&7+<*$-)+#'%#7+&#,>G
=*^-)-+ ,*%-&9Z+ 3)1$*,#)+ 3*'"*@-$-'+ ,-'+ 3*'"&)&@-'+ $3*$&@*'+
M1I&,NLO+++-,-9-(+$*<-"-&+<*)&7#%H+
LH$ A-$#77-'+*,)#(+2'+.-'"+<*)&$&+$3*$&@*'+7*+,-9-@+39-$%&7+79&3+
.-'"+%*9-(+,&<*)&+%&$#H
SH$ A-$#77-'+ 39-$%&7+ 79&3+ <*)&$&+ $3*$&@*'+ 7*+ ,-9-@+ *,)#2#Za2"#-2#((.'H
PH$ =#$#'+ "*'- =%*K+ <*7#+ @&'&@-9+ ]+ <#-(+ 3-,-+ <-"&-'+ <-5-(Z+
$-@3&'"Z+ ,-'+ -%-$+ *,)#2#Za2"#2#((.'+ ,&+ ,-9-@+ *##.- 2#ZH+ J&9-+
3*)8-9-'-'+rSR+8-@Z+9*<&(+<-&7+@*'-@<-(+8#@9-(+"*'-=%*K+.-'"+
,&@-$#77-'+7*+,-9-@+*##.-2#ZH+
RH$ A-$#77-'+*,)#2#ZR2"#-2#((.'+7*+,-9-@+*##.-2#Z+,-'+%#%#3+<-"&-'+
-%-$+,*'"-'+$-%#+<#-(+"*'-=%*KH+>*%-77-'+%*)@1@*%*)+,&+,-9-@'.-H WH$ >*%-77-'+&'$#9-%1)H+
]H$ A-$#77-'+:1)@#9&)+%*)7-&%+7*+,-9-@+39-$%&7+%*)3&$-(Z+$*)%-7-'+
,-9-@+*##.-2#ZH
VH$ D#%#3+*##.-2#Z+,-'+9-7<-'H
YH$ J*)&+9-<*9+-9-@-%+&'$%&%#$&+3*'"&)&@+$3*$&@*'+,-'+9-<1)-%1)&#@+
3*@*)&7$-+
OH$ =3*$&@*'+ $&-3+ ,&7&)&@H+ =3*$&@*'+ ,-9-@+ *##.- 2#Z+ ,*'"-'+
"*'-PEDOMAN 131
PEMERIKSAAN PCR SARS-CoV-2 BAGI PETUGAS LABORATORIUM
=%*K- <*7#+ ,-3-%+ <*)%-(-'+ @-7$&@-9+ $*7&%-)+ RY+ 8-@+ [,-9-@+
)*'%-'"+ 5-7%#+ &'&Z+ ,&(-)-37-'+ $#(#+ ,&+ ,-9-@+ *##.- 2#Z+ @-$&(+
<*)7&$-)+SNN+Y1M\H
4*'"&)&@-'+ $3*$&@*'+ 7*+ 9-<1)-%1)&#@+ 3*@*)&7$-+ ,-3-%+
@*'""#'-7-'+ 8-$-+ 7#)&)+ 5##,- (#- 5##,G- 4-,-+ 71',&$&+ .-'"+
@*@*)9#7-'+ 3*'"&)&@-'+ =#,(- (#- =#,(Z+ 3*%#"-$+ 0&'-$+ ?*$*(-%-'+
,-3-%+<*)711),&'-$&+,*'"-'+3*%#"-$+??4+$*%*@3-%+,-'+9-<1)-%1)&#@+
3*@*)&7$-H+=3*$&@*'+$*"*)-+,&7&)&@7-'+7*+9-<1)-%1)&#@+3*@*)&7$-+
3-9&'"+9-@-+LKSR+8-@H
%0K0K$;4P424.$@F$%1.V3HL4.4.$*L123H1.$/3$-4Q0E4I0E3NH +F$ *1Q1GNH$L1H1E3K244.$%5:
=3*$&@*'+<-&7'.-+,&3*)&7$-+9-'"$#'"+$--%+%&<-+,&+9-<1)-%1)&#@H+
/-@#'Z+ 8&7-+ $3*$&@*'+ @-$&(+ @*'"-'%)*+ + #'%#7+ <&$-+ ,&7*)8-7-'Z++
@*'"-^#+7*3-,-+D-<*9+RHLZ+3*'.&@3-'-'+$3*$&@*'+#$-3+'-$1:-)&'"+
,-'+1)1:-)&'"Z+<&$-+,&$&@3-'+%*)9*<&(+,-(#9#+3-,-+$#(#e
LH+ SNY1MZ+(&'""-+LS+(-)&+$*8-7+%-'""-9+3*'"-@<&9-'+$3*$&@*'j+
SH+ NVU1MZ+8&7-+$#,-(+9*<&(+,-)&+LS+(-)&+$*8-7+%-'""-9+3*'"-@<&9-'+
$3*$&@*'H+
2&',-)&+ @*9-7#7-'+ <*7#N^-&)+ 3-,-+ $3*$&@*'+ #$-3+ '-$1:-)&'"+
,-'+1)1:-)&'"+$*<*9#@+3*@*)&7$--'+4MCH+4)1$*$+<*7#+^-&)+3-,-+
$3*$&@*'+,-3-%+@*'#)#'7-'+7#-9&%-$+$3*$&@*'+[;2TZ+SUSU\H
;F$ *1I1G4P$L1H1E3K244.$%5:
?*%&7-+(-$&9+3*@*)&7$--'+4MC+%*9-(+$*9*$-&+,-'+,&$&@3#97-'+
$*^-)-+ I-9&,Z+ + $-@3*9+ $&$-+ ,&$&@3-'+ 3-,-+ $#(#+ NVU1MH+ C/!+ (-$&9+
*7$%)-7$&+(-)#$+,&$&@3-'+3-,-+$#(#+NVU1M+#'%#7+3*'.&@3-'-'+9-@-H