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PENUTUP 5.1 Kesimpulan

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Berdasarkan data yang diperoleh, dapat disimpulkan bahwa kadar glukosa, sukrosa, lipid dan protein sampel kedelai menunjukkan nilai konsentrasi lebih tinggi dibandingkan dengan jagung. Sedangkan kadar pati atau starch pada kedelai lebih rendah dibandingkan dengan sampel jagung. Kedelai memiliki kadar glukosa sebesar 45,78 µg/µl, sukrosa 5,3 µg/µl, pati atau starch 10,16 µg/µl, kadar protein sebesar 75,12µg/µl dan kandungan lipid sebesar 9,89 g/100g. Jagung memiliki kadar glukosa sebesar 30,49 µg/µl, sukrosa 3,84 µg/µl, pati atau starch 16,81 µg/µl, kadar protein sebesar 5,88µg/µl dan kandungan lipid yang hanya 3,37 g/100g. Pola protein yang terlihat pada hasil visualisasi SDS-PAGE antara jagung dan kedelai memiliki pola protein yang berbeda, namun ukuran pastinya belum bisa diketahui karena markernya tidak terlihat. Sedangkan pada aktivitas uji enzim, isolat Aspergillus sp. yang ditumbuhkan pada media jagung memiliki nilai aktivitas enzim yang tinggi yaitu 0,361, sedangkan pada media kedelai hanya 0,074 hal ini menunjukkan bahwa aktivitas selulasenya tinggi. Selain itu analisis secara kualitatif juga menunjukkan bahwa aktivitas amilase isolat Aspergillus sp. tinggi karena terbentuk warna biru keunguan ketika ditetesi dengan Iodine.

5.2 Saran

Beberapa hasil pengukuran kadar glukosa dan selulosa yang kurang sesuai jika dibandingkan dengan literatur dimungkinkan karena proses homogenisasi yang tidak maksimal oleh karena itu, sebaiknya perlu diperhatikan proses homogenisasi agar molekul seperti glukosa dan sukrosa terekstraksi secara optimal, selain itu pada uji kualitatif perlu dilakukan pengukuran diameter zona bening dan diameter koloni agar diperoleh kisaran aktivitas enzimnya.

Daftar Pustaka

Cambell N.A., Reece J. B., Urry L. A., Cain M. L., Wasserman S. A.,

MinorskyP.V., and Jackson, R.B. 2012. Biologi, edisi 8, jilid 1. Jakarta: Erlangga

Caunt , Christopher J. , Andrew M. Kidger , and Stephen M. Keyse. 2016. Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specifi city Mitogen-Activated Protein Phosphatases (MKPs). Protein Tyrosine Phosphatases: Methods and Protocols, Methods in Molecular Biology, vol. 1447, DOI 10.1007/978-1-4939-3746-2_12 Cheirsilp, Benjamas and Salwa Torpee. 2012. Enhanced growth and lipid

production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation. Bioresource Technology 110: 510–516

Cheng, Y., Haiming Wei , Rui Sun, Zhigang Tian , and Xiaodong Zheng. 2016. Rapid method for protein quantitation by Bradford assay after elimination of the interference of polysorbate 80. Analytical Biochemistry 494 : 37-39 Cole, Marsha R., Gillian Eggleston, Audrey Gilbert and Yoo Jin Chung. 2016.

Development of an analytical method to measure insoluble and soluble starch in sugarcane and sweet sorghum products. Journal of Food Chemistry 190: 50-59

Cruz, Mutya, Miao Wang, Jessica Frisch-Daiello and Xianlin Han. 2016. Improved Butanol–Methanol (BUME) Method by Replacing Acetic Acid for Lipid Extraction of Biological Samples. Journal of Lipids 51:887–896 Datir, Sagar and Snehal Joshi. 2016. The contribution of sucrose metabolism

enzymes to sucrose accumulation in sugarcane (Saccharum officinarum L.) genotypes. Indian Jounal of Plant Physiology 21(1):76–82

Eggers, Lars F. and Dominik Schwudke. 2016. Lipid Extraction: Basics of the Methyl-tert-Butyl Ether Extraction. Encyclopedia of Lipidomics DOI 10.1007/978-94-007-7864-1_96-1

Evangelista, CM. and M.A.B. Regitano-D'Arce. 2009. Spectrophotometric analysis of the lipid fraction of microwave heated and soaked soybeans. Grasas y Aceites Vol. 50. Fase. 1: 22-25

Fery, S. Erwin, S. dan Trimarsanto, H. 2010. Analisis Perbandingan Struktur ProteinFOXP2 Normal dengan Mutan R553H. Undergraduate thesis. Facultyof Biotechnology Atma Jaya Catholic University of Indonesia

Golunski,Simone Maria, Luisa Sala, Marceli Fernandes Silva, Rogério Marcos Dallago, Jéssica Mulinari, Altemir José Mossi, Adriano Brandelli, Susana

Juliano Kalil, Marco Di Luccio, and Helen Treichel. 2016. Interference of salts used on aqueous two-phase systems on thequantification of total proteins. International Journal of Biological Macromolecules 83: 30–33 Goncavales, C., Rosa Maria R.J., Nelma Gomes, Jose A. Teixeira and Isabel Belo.

2010. Adaptation of dinitrosalicylic acid method to microtiter plates. Journal ofAnalytical Method. DOI: 10.1039/c0ay00525h

Gondim, C. de Souza, Roberto C. S. de Souza, Maurina de Paula P. E Palhares, Roberto G. J., and Scheila V. C. de Souza. 2015. Performance improvement and single laboratory validation of classical qualitative methods for the detection of adulterants in milk: starch, chloride and sucrose. Journal of Analytical Method. DOI 10.1039/c5ay01822f

Gupta, R.B. and K.W. Shepherd. 2000. Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin. Theor Appl Genetic 80: 65-74

Jeffries, T. W. (1994). Biodegradation of Lignin and Hemicelluloses, 233–277. Liu, Weiguo., Yuchuan Deng, Sajad Hussain, Junlin Zou, Jin Yuan, Ling Luo,

Chengyu Yang, Xiaoqing Yuan, and Wenyu Yang. 2016. Relationship between cellulose accumulation and lodging resistance in the stem of relay intercropped soybean (Glycine max (L.) Merr.). Journal of Field Crops Research 196: 261-267

Merriman, L.A., C. L. Walk, C. M. Parsons, and H. H. Stein. 2016. Effects of tallow, choice white grease, palm oil, corn oil, or soybean oil on apparent total tract digestibility of minerals in diets fed to growing pigs. Journal of

Animal Science.94:4231–4238. doi:10.2527/jas2016-0682

Nakamura, Sumiko., Junji Katsura, Kiyoko Kato, and Kenichi Ohtsubo. 2015. Development of formulae for estimating amylose content and resistant starch content based on the pasting properties measured by RVA of Japonica polished rice and starch. Journal of Bioscience, Biotechnology, and

Biochemistry Vol. 80 No. 2, 329-340

Olennikov, D.N and L.M. Tankhaeva. 2011. A Quantitative Assay for Total Fructans in Burdock (Arctium spp.) Roots. Russian Journal of Bioorganic Chemistry Vol. 37, No. 7, pp. 893–898

Madhu, Sheri., Hayden A. Evans, Vicky V. T. Doan-Nguyen, John G. Labram, Guang Wu, Michael L. Chabinyc, Ram Seshadri, and Fred Wudl. 2016. Infinite Polyiodide Chains in the Pyrroloperylene–Iodine Complex:Insights into the Starch–Iodine and Perylene–Iodine Complexes. Angew Chem 128, 1 – 5. http://dx.doi.org/10.1002/anie.201601585

Moat, A. G., Foster, J. W., & Spector, M. P. 2002. Microbial Physiology (Fourth.). A John Wiley & Sons, INC.

Nie, Jinfang., Tom Brown and Yun Zhang. 2013. New two dimensional liquid-phase colorimetric assay based on old iodine-starch complexation for simple, low-cost, portable, naked-eye detection and quantification of analytes. DOI: 10.1039/x0xx00000x.

Pérez, J. Muñoz-Dorado, J. de la Rubia, T & Martínez, J. 2002. Biodegradation and Biological Treatments of Cellulose, Hemicellulose and Lignin: An Overview. International Microbiology : The Official Journal of the Spanish  Society for Microbiology, 5(2) : 53–63.

Sigismund, Sara and Simona Polo. 2016. Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein. Proteostasis: Methods and Protocols, Methods in Molecular Biology, vol. 1449, DOI 10.1007/978-1-4939-3756-1_6

Teixeira, Ricardo S.S., Ayla Sant’Ana da Silva, Viridiana Santana F.L., and Elba Pinto da Silva Bon. 2012. Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity. Carbohydrate Research 363 : 33–37

Torres, Sebastian., Jorge E. Sayago, Roxana M. Ordones and Maria Ines Isla. 2011. A colorimetric method to quantify endo-polygalacturonase activity. Enzyme and Microbial Technology 48: 123–128

Verma, A. K., S. K. Upadhyay, P. C. Verma, S. Solomon and S. B. Singh. 2010. Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars. Journal of Plant Biology.doi:10.1111/j.1438-8677.2010.00379.x

Wang, Yan, Qing-Yun Zheng, Zhen-Ru Guo, Yuan-Yuan Qiao, Yong-Li Cao, Cai-Hong Liu, Bin-Jie Xu, Zheng-YuanWei, Lu-Juan Zong, Chen Chen, Ya-Nan Han, Qing Chen, Xi Gong, Shi-Yu Hua , You-Liang Zheng, Peng-Fei Qi, Yu-Ming Wei, Xiu-Jin Lan, and Ji-RuiWang. 2016. A missense mutation affects the mobility of high molecular weight glutenin Dy10 subunit in SDS-PAGE. Agri Gene 2 : 1–4

Wang, H., Zesheng Zhang, Liya Liang, Shengping Wen, Chunyan Liu, and Xiaojun Xu. 2010. A comparative study of high-performance liquid chromatography and colorimetric method for inulin determination. Europe Food Research and Technology 230:701–706. DOI 10.1007/s00217-009-1208-8

Zhu, Liang., Jiaheng Zhou, Meile Lv, Haitian Yu, Hang Zhao, and Xiangyang Xu. 2015. Specific component comparison of extracellular polymeric substances

(EPS) in flocs and granular sludge using EEM and SDS-PAGE. Chemosphere 121 : 26–32

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