[BPS] Badan Pusat Statistik. 2015. Produksi cabai besar, cabai rawit, dan bawang merah tahun 2014. Berita Resmi Statistik: 71/08/XVIII. Jakarta (ID): Badan Pusat Statistik.
[KEMENTAN] Kementerian Pertanian Republik Indonesia. 2015. Rencana Strategis Kementerian Pertanian 2015-2019. Jakarta (ID): Kementerian Pertanian Republik Indonesia.
Agrios GN. 2005. Plant Pathology 5th Ed. Oxford (GB) :Elsevier Academic Pr. Arnold AE, Mejía LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA. 2003.
Fungal endophytes limit pathogen damage in a tropical tress. PNAS
100:15649-15654.
Bailey JA, O’Connell RJ, Pring RJ, Nash C. 1992. Infection strategies of
Colletotrichum species. In Colletotrichum. Biology, Pathology and Control. Bailey JA, Jeger MJ, editor. Wallingford (GB): CABI.
Barnett HL, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi. Fourth Edition. Saint Paul (US): The American Phytopathological Society Pr. Chaisemsaeng P, Mongkolthanaruk W, Bunyatratchata W. 2013. Screening and
potential for biological control of anthracnose disease (Colletotrichum capsici) on chili fruits by yeast isolates. J Life Sci and Technol. 4(1): 202-204.
Chanchaichaovivat A, Ruenwongsa P, Panijpan B. 2007. Screening and identification of yeast strain from fruits and vegetables: potencial for biological control of postharvest chili antrachnose Colletotrichum capsici.
Biol Cont. 42:326-335.
Clarke BB, White Jr JF, Hurley RH, Torres MS, Sun S, Huff DR, 2006. Endophyte-mediated suppression of dollar spot disease in fine fescues. Plant Dis. 90: 994-998.
Clay K, Schardl C. 2002. Evolutionary origins and ecological consequences of endophyte symbiosis with grasses. American Naturalist 160:99-127.
Cook RJ, Baker KF. 1993. The Nature and Practice of Biological Control of Plant Pathogens. St Paul (US): APS Pr.
Dai C, Yu B, Li X. 2008. Screening of endophytic fungi that promote the growth of Euphorbia pekinensis. Afr J Biotechnol. 7:3505-3510.
Deising HB, Werner S, Wernitz M. 2000. The role of fungal appressoria in plant infection. Microb and Infect. 2:1631–1641.
Dingle J, Mc Gee PA. 2003. Some endophyte fungi reduce the density of pustules of Puccinia recondi f.sp. tritici in wheat. Mycol Res. 107:310-316.
Garg R, Kumar S, Kumar R, Loganathan M, Saha S, Kumar S, Rai AB, Roy BK. 2013. Novel source of resistance and differential reactions on chilli fruit infected by Colletotrichum capsici. Aus Plant Pathol. 42:227-233. doi:10.1007/s13313-012-0194-7.
Genarro M, Gonthier P, Nicolotti G. 2003. Fungal endophytic communities in healty and declining Quercus robur L. and Q. cerris L. tress in Northern Italy.
J Phytopathol. 151:529-534.
Ginting RCB, Sukarno N, Widyastuti U, Darusman LK, Kanaya S. 2013. Diversity of endophytic fungi from red ginger (Zingiber officinale rosc.) plant and their
21 inhibitory effect to Fusarium oxysporum plant pathogenic fungi. HAYATI 20 (3):127-137.
Gundel PE, Helander M, Casas C, Hamilton C, Faeth S, Saikkonen K. 2012.
Neotyphodium fungal endophyte in tall fescue (Schedonorus phoenix): a comparison of three Northern European wild populations and the cultivar Kentuky-31. Fungal Divers. 1-10.
Gundel PE, Rudgers JA, Ghersa CM. 2011. Incorporating the process of vertical transmission into understanding of host symbiont dynamics. Oikos 120: 1121-1128
Hamayun M, Khan SA, Khan AL, Tang DS, Hussain J, Ahmad B, Anwar Y, Lee IJ. 2010. Growth promotion of cucumber by pure cultures of gibberellins-producting Phoma sp. GAH7. J Microbiol Biotechnol. 26:889-894.
Hammerschmidt R, Nuckles EM, Kuc J. 1982. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiol Plant Pathol. 20:73-82.
Hariati N. 2007. Analisis keragaman 23 genotipe cabai (Capsicum sp.) berdasarkan penampakan fenotipik serta ketahanannya terhadap penyakit antraknosa (Colletotrichum sp.) [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Hospenthal DR, Rinaldi MG. 2008. Diagnosis and Treatment of Human Mycoses.Georgiev VS, editor. New Jersey (US): Humana Pers Inc.
Hyde KD, Soytong K. 2008. The fungal endophyte dilemma. Fungal Divers.
33:163-173.
Inacio J, Ludwig W, Spencer-Martins I, Fonseca A. 2010. Assessment of phylloplane yeasts on selected Mediterranean plants by FISH with group and species oligonucleotide probes. FEMS Microbiol Ecol. 71:61-72.
Istikorini Y. 2008. Potensi cendawan endofit untuk mengendalikan penyakit antraknosa pada cabai (Capsicum annuum L.) [disertasi]. Bogor (ID) : Institut Pertanian Bogor.
Joshee S, Paulus BC, Park D, Johnston PR. 2009. Diversity and distribution of fungal foliar endophytes in New Zealand Podocarpaceae. Mycol Res. 113: 1003-1015.
Kanchana-udomkan C, Taylor PWJ, Mongkol-porn O. 2004. Development of a bioassay to study anthracnose infection of Capsicum chinense Jacq. fruit caused by Colletotrichum capsici. Thai J Agric Sci. 37: 293-297.
Khan SA, Hamayun M, Khan Al, Lee IJ, Shinwari ZK, Kim J. 2012. Isolation of plant growth promoting fungi from dicots inhabiting coastal sand dunes of Korea. J Bot. 44:1453-1460.
Kiewnick S, Sikora RA.2006. Biological control of the root-knot nematode
Meloidogyne incognita by Paecilomyces lilacinus strain 251. Bio Cont.
38:179-187.
Kim WK, Cho EK, Lee ES. 1985. Variations of the anthracnose pathogen of pepper,
Colletotrichum gloeosporioides Perez. in morphology, pathogenicity and cultural characteristics. J Plant Pathol.1:211-212.
Kim CK, Eo JK, Eom AH. 2013. Diversity and seasonal variation of endophytic fungi isolated from three conifers in Mt. Taehwa Korea. Microbiology 41(2): 82-85.
22
Kim KD, BJ Oh, Yang J. 1999. Differential interaction of a Colletotrichum gloeosporiodes isolate with green and red pepper fruits. Phytoparasitica
27(2):1-10.
Kleemann J, Rincon-Rivera LJ, Takahara H, Neumann U, van Themaat EVL, et al.
2012. Sequential delivery of host-induced virulence effectors by appressoria and intracellular hyphae of the phytopathogen Colletotrichum higginsianum.
PLoS Pathogens 8(4): e1002643. doi:10.1371/journal.ppat.1002643.
Krauss J, Harri DA, Bush L, Power SA, Muller CB. 2007. Fungal grass endophytes, grass cultivars, nitrogen deposition and the associations with colonizing insects [Internet]. [diunduh 2015 Des 15] https://www.grassland.org.-nz/publications/nzgrassland_publication_2424.pdf
Lehtonen P, Helander M, Saikkonen K. 2005. Are endophyte-mediated effects on herbivores conditional on soil nutrients?. Oecologia 142:38-45.
Li HM, Sullivan R, Moy M, Kobayashi DY, Belanger FC. 2004. Expression of a novel chitinase by the fungal endophyte in Poa ampla. Mycologia 96 (3): 526-536.
Lu G, Cannon PF, Reid A, Simmons CM. 2004. Diversity and molecular relationships of endophytic Colletotrichum isolates from the Iwokrama Forest Reserve, Guyana. Mycol Res. 108:53-63.
Mahasuk P, Khumpeng N, Wasee S, Taylor PWJ, Mongkolporn O. 2008. Inheritance of resistance to anthracnose (Colletotrichum capsici) at seedling and fruiting stages in chili pepper (Capsicum spp.). Plant Breeding 128: 701-706.
Mejía LC, Rojas EI, Maynard Z, Bael SV, Arnold AE, Hebbar P, Samuels GJ, Robbins N, Herre EA. 2008. Endophytic fungi as biocontrol agents of
Theobroma cacao pathogens. Bio Cont. 46:4-14.
Mendgen K, Deising H. 1993. Infection structures of fungal plant pathogens-a cytological and physiological evaluation. New Phytol. 124:193-213.
Montri P, Taylor PWJ, Mongkolporn O. 2009. Pathotypes of Colletotrichum capsici the causal agent of chili antrachnose, in Thailand. Plant Dis. 93:17-20.
Nicholson RL, Moraes WBC. 1980. Survival of Colletotrichum graminicola: importance of the spore matrix. Phytopathology 70:255-261.
Nurahmi E, Mahmud T, Rossiana SS. 2011. Efektifitas pupuk organik terhadap pertumbuhan dan hasil cabai merah. J Floratek. 6:158-164.
O’Connell RJ, Perfect S, Hughes B, Carzaniga R, Bailey JA, et al. 2000. Dissecting the cell biology of Colletotrichum infection processes. Di dalam:
Colletotrichum. HostSpecificity, Pathology and Host-Pathogen Interaction.
Prusky D, Freeman S, Dickman MB, editor. St Paul (US):APS Pr. hlm 57-77. O’Connell RJ, Thon MR, Hacquard S, Amyotte SG, Kleemann J, et al. 2012.
Life-style transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses. Nature Gen. 44(9):160-167. doi:10.1038/ng.2372.
Pakdeevaraporn P, Wasee S, Taylor PWJ, Mongkolporn O. 2005. Inheritance of resistance to anthracnose caused by Colletotrichum capsici in Capsicum.
23 Pérez LI, Gundel PE, Ghersa CM, Omacini M. 2013. Family issues: fungal endophyte protects host grass from the closely related pathogen Claviceps purpurea. Fungal Ecol. 6:379-386.
Popay AJ, Bonos SA. 2008. Biotic responses in endophytic grasses. Di dalam:
Neotyphodium in Cool-Season Grasses. Roberts CA, West CP, Spiers DE, editor. Los Angeles (US): Blackwell Publishing. hlm 163-185.
Prusky D, Plumbley RA. 1992. Quiescent infections of Colletotrichum in tropical and subtropical fruit. Di dalam: Colletotrichum. Biology, Pathology and Control .Bailey JA, Jeger MJ, editor. Wallingford (UK): CABI.
Rahman MA, Rahman MM, Azad AK, Alam MF. 2011. Inhibitory effect of different plant extracts and antifungal metabolites of Trichoderma strains on the conidial germination and germ tube growth of Colletotrichum capsici
causing chili anthracnose. IJAAR 1(1):20-28. ISSN: 2223-7054.
Raj TS, Christopher DJ. 2009. Effect of bio-control agents and fungicides against
Colletotrichum capsici causing fruit rot of chilli. Ann Plant Protect Sci.
17:143-145.
Raj TS, Christopher DJ, Suji AH. 2013. Evaluation of virulence and methods of inoculation of Colletotrichum capsici (SYD) Butler and Bisby. Int J Agric Sci. 9(2):802-805.
Raj TS, Christopher DJ, Suji HA. 2014. Morphological, pathogenic and genetic variability in Colletotrichum Capsici causing fruit rot of chili in Tamil Nadu, India. Afr J of Biotechnol. 13(17):1786-1790.
Rasmussen S, Parsons AJ, Popay A, Xue H., Newman JA. 2008. Plant-endophyte-herbivore interactions: more than just alkaloids?. Plant Signal and Behav.
3:974-977.
Rodriguez R, Redman R. 2008. More than 400 million years of evolution and some plants still can't make it on their own: plant stress tolerance via fungal symbiosis. J of Exp Bot. 59(5):1109-1114.
Rodriguez RJ, White Jr JF, Arnold AE, Redman RS. 2009. Fungal endophytes: diversity and functional roles. New Phytol. 182:314-330. doi:10.1111/j.1469-8137.2009.02773.x.
Rojas EI, Rehner SA, Samuels GJ, Van Bael SA, Herre EA, et al. 2010.
Colletotrichum gloeosporioides s.l. associated with Theobroma cacao and other plants in Panama: multilocus phylogenies distinguish pathogen and endophyte clades. Mycologia 102:1318–1338.
Saikkonen K, Saari S, Helander M. 2010. Defensive mutualism between plants and endophytic fungi?. Fungal Divers. 41:101-113. doi:10.1007/s13225-010-0023-7.
Schulz B, Boyle C, Draeger S, Rommert SK, Krohn K. 2002. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res.
106:996-1004.
Schulz B, Boyle C. 2005. The endophytic continuum. Mycologia 109(6):661-686. Schulz B, Rommert AK, Dammann U, Aust HJ, Strack D. 1999. The endophyte–
host interaction: a balanced antagonism?. Mycol Res.10:1275-1283.
Sharma G, Pinnaka AK, Shenoy BD. 2014. Infra-specific diversity of
Colletotrichum truncatum associated with chili anthracnose in India based on microsatelite marker analysis. Arch of Phytopathol and Plant Prot.
24
Siddharha B, Prabhakar P, Surya NM, Murty USN, Venkateswarlu Y. 2009. Secondary metabolites of Curvularia oryzae MTCC 2605 [abstrak]. Rec. of Nat. Prod. 3(4):204-208. [Internet]. [diunduh 2015 Des 29]. Tersedia pada: http://14.139.64.106:8080/jspui/handle/123456789/6862.
Stirling AM, Pegg KP, Hayward AC, Stirling GR. 2000. Effect of copper fungicide on Colletotrichumgloeosporioides and other microorganism on avocado and fruit. Aus J Agric Res. 50:1459-1468.
Sutton BC. 1992. The genus Glomerella and its anamorph Colletotrichum. Dalam:
Colletotrichum: Biology, Pathology and Control. Bailey JA, Jeger MJ, editor. Wallingford (UK): CABI.
Tan RX, Zou WX. 2001. Endophytes : A rich source of functional metabolites. Nat. Prod. Rep. 18:448-459.
Than PP, Prihastuti H, Phoulivong S, Taylor PWJ, Hyde KD. 2008. Review: Chili anthracnose disease caused by Colletotrichum species. J of Zhejiang Univ Sci.
9(10):764-778.
Tian P, Nan Z, Li C, Spangenberg G. 2008. Effect of the endophyte Neotyphodium lolii on susceptibility and host physiological response of perennial rye grass to fungal pathogens. EuroJ Plant Pathol. 122:593-602.
Uchitel A, Omacini M, Chaneton EJ. 2011. Inherited fungal symbionts enhance establishment of an invasive annual grass across successional habitats.
Oecologia 165:465-475.
Vasudevan P, Reddy MS, Kavitha S, Velusamy P, Paul RSD. 2002. Role of biological preparations in enhancement of rice seedling growth and grain yield. Curr Sci. 83:1140-1143.
Waller JM, Lenné JM, Waller SJ. 2002. Plant Pathologists’s Pocketbook. Wallingford (UK): CABI.
Wang JW, Wu JH, Huang YW, Tan RX. 2006. Laccase production by Monotospora
sp. an endophytic fungus in Cynodon dactylon. Biores Technol. 97:786-789. Watanabe T . 2002. Pictorial Atlas Of Soil And Seed Fungi. Second Edition. Boca
Raton (US): CRC Pr.
Wharton PS, Diéguez-Uribeondo J. 2004. The biology of Colletotrichum acutatum.
Anales del Jardín Botánico de Madrid 61(1):3-22.
White Jr, J.F., Torres, MS, 2010. Is plant endophyte-mediated defensive mutualism the result of oxidative stress protection?. Plant Physiol.138:440-446.
Wilia W. 2010. Potensi cendawan endofit dan khamir untuk mengendalikan penyakit antraknosa (Colletotrichum acutatum L.) Pada Tanaman Cabai [tesis]. Bogor (ID): Institut Pertanian Bogor.
Wusani M. 2004. Pewarisan karakter ketahanan pada cabai (Capsicum annuum x
Capsicum chinense) terhadap penyakit antraknosa (Colletotrichum gloeosporioides Penz.) [tesis]. Bogor (ID): Intitut Pertanian Bogor.
Yuan ZL, Su ZZ, Mao LJ, Peng YQ, Yang GM, Lin FC, Zhang CL. 2011. Distinctive endophytic fungal assemblage in stems of wild rice (Oryza granulata) in China with special reference to two species of Muscodor
(Xylariaceae). J Microbiol. 49:15-23.
Yan N, Wang XQ, Xu XF, Guo DP, Wang ZD, Zhang JZ, Hyde KD, Liu HL. 2013. Plant growth and photosynthetic performance of Ziziana latifolia are altered by endophytic Ustilago esculenta infection. Physiol and Moleculer plant Pathol. 83:75-83.
25 Zhang HW, YC Song, RX Tan. 2006. Biology and chemistry of endophytes. Nat.
26
27 Lampiran 1 Lokasi pengambilan sampel tanaman cabai sehat (A) Cianjur, (B)
Bogor, (C) Garut
A
B
28
29 Lampiran 3 Hasil isolasi cendawan patogen penyebab antraknosa pada cabai merah, (A, B) struktur morfologi C. capsici pengamatan dengan mikroskop compound pada perbesaran 400x, dan (C) koloni cendawan C. capsici pada media PDA
Lampiran 4 Gejala yang muncul pada kecambah cabai merah dalam uji patogenisitas, (A dan B) kecambah nekrotik, (C) benih tidak berkecambah, (D) kecambah tidak berkembang, (E) Kecambah pada kontrol, dan (F) Kecambah pada perlakuan cendawan endofit non patogenik A B C A B C E D F
30
Lampiran 5 Hasil identifikasi cendawan endofit berpotensi non fitopatogenik
Kode Isolat Makroskopik Mikroskopik Hasil Identifikasi
E1Bh5G1 Miselia steril
E2A3B2 Miselia steril E3Cb7B2 Curvularia sp. E4D5G1 Colletotrichum gloeosporioides E5Bh4G1 Miselia steril E6D9B2 Paecilomyces sp.
31 Lampiran 5 Hasil identifikasi cendawan endofit berpotensi non patogenik
Kode Isolat Makroskopik Mikroskopik Hasil Identifikasi
E7B8B2 Miselia steril
E8B3G1 Paecilomyces sp.
E9D10G2 Verticillium sp.
E10D6G1 Fusarium solani
non patogenik
E11D9B2
32
Lampiran 5 Hasil identifikasi cendawan endofit berpotensi non patogenik
Kode Isolat Makroskopik Mikroskopik Hasil Identifikasi
E12D6G2 Verticillium sp.
E13A7G1
Miselia steril
33 Lampiran 6 Uji kultur ganda (A) mekanisme antibiosis, (B) mekanisme lisis
A
34
Lampiran 7 Keragaan pertumbuhan tanaman dengan perlakuan endofit pada 35 hari setelah pindah tanam
35 Lampiran 8 Lahan percobaan (A) Dramaga, (B) Desa Loa-Tamansari
B A
37
RIWAYAT HIDUP
Penulis lahir di kota Klaten – Jawa Tengah pada tanggal 05 Agustus 1985, dan merupakan putri pertama dari Bapak Wahyono dan Ibu Rubiyati. Penulis lulus dari SMU Islam Panglima Besar Sudirman Jakarta pada tahun 2004. Pendidikan sarjana pada Departemen Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor – Jawa Barat, dan menyelesaikan studi pada tahun 2010.
Pada tahun 2010 sampai 2011 penulis bekerja di IPB Culture Collection
(IPBCC), sebagai anggota analis bagian mikologi – Bogor. Pada tahun 2011 penulis berkesempatan untuk melanjutkan studi S2 pada program studi Fitopatologi di Institut Pertanian Bogor. Pada tahun 2011 penulis berkesempatan untuk melanjutkan studi S2 pada program studi Fitopatologi di Institut Pertanian Bogor.