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LAMPIRAN
Lampiran 1. Daftar Isolat Jamur Endofit
No. Kode Isolat Asal Isolat
1 JRD 1A Hedychium coronarium
2 JRD 2A Hedychium coronarium
3 JRD 3A Hedychium coronarium
4 JRD 2C Hedychium coronarium
5 JRE 1A Hedychium coronarium
6 JRE 2A Hedychium coronarium
7 JRE 1B Hedychium coronarium
8 JRE 2B Hedychium coronarium
9 JRE 4B Hedychium coronarium
10 JRL 1A Amomum centrocephalum
11 JRL 2A Amomum centrocephalum
12 JRL 3A Amomum centrocephalum
13 JRL 4A Amomum centrocephalum
14 JRL 1B Amomum centrocephalum
15 JRL 2D Amomum centrocephalum
16 JRN 2A Etlingera sp.
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Lampiran 2. Komposisi Medium
3.1 Medium Garam Minimum Kitin (MGMK) Agar
KH2PO4 0,3 gr
K2HPO4 0,7 gr
MgSO4.7H2O 0,5 gr
FeSO4. 7H2O 0,01 gr
ZnSO4 0,001 gr
MnCl2 0,001 gr
Koloidal kitin 1% (b/v) 72,7 ml
Agar 15,0 gr
pH 7,0
Akuades 1000 ml
3.2 Medium Garam Minimum Kitin (MGMK) Cair
KH2PO4 0,3 gr
K2HPO4 0,7 gr
MgSO4.7H2O 0,5 gr
FeSO4. 7H2O 0,01 gr
ZnSO4 0,001 gr
MnCl2 0,001 gr
Koloidal kitin 1% (b/v) 72,7 ml
pH 7,0
Akuades 1000 ml
Cara Pembuatan:
Semua bahan dicampur dan ditambahkan akuades sampai volumenya menjadi 1000 ml. Diatur pH sampai 7 dengan menambahkan NaOH 0,1 N atau HCl 0,1 N. Setelah dicapai pH yang diinginkan, media disterilkan dengan autoklaf pada suhu 121⁰C selama 15 menit.
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Lampiran 3. Pembuatan Reagen 3,5-Dinitrosalicyclic Acid (DNS)
Reagen DNS dibuat dengan melarutkan 1 gr 3,5-Dinitrosalicyclic Acid dalam 50 ml akuades steril, kemudian ditambahkan 30 gr Sodium Potassium Tartarate hingga campuran bewarna kuning susu. Selanjutnya ditambahkan 20 ml NaOH 2 N, warna campuran akan berubah menjadi oranye transparan. Tambahkan akuades hingga volume akhir menjadi 100 ml, selanjutnya simpan dalam botol gelap pada kondisi dingin.
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Lampiran 4. Kurva Standar GlcNac dan Bovine Serum Albumin
y = 0.001x - 0.0267
0 200 400 600 800 1000 1200
Absorbansi (540 nm)