43 BAB V
44
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50 LAMPIRAN
Lampiran 1. Alat, Bahan, dan Instrumen Penelitian
Teh Hijau
Sebuk Baku AgNO3 Timbangan Analitik
Hotplate
Water Purificator Vortex
Spektrofotometer UV-Vis Double Beam
Kuvet Spektrofotometer
Mikropipet
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Centrifuge Tube Sentriugator
Kertas Indikator pH
pH Meter, Larutan Dapar pH 4 dan 7
Sonicator Bath
Ketamine HCl
Veet® dan Spatula
Hansaplast Spray® Gunting Bedah
52 Biopsy Punch 4 mm
Pinset
Spuit Injeksi
Kertas Milimeter Blok
Kloroform Formalin Buffer pH 7
53
Lampiran 2. Hasil Nanosilver Sebelum dan Sesudah Purifikasi Perlakuan Sebelum Purifikasi Sesudah Purifikasi
P1
P2
P3
54
Lampiran 3. Panjang Gelombang dan Persen Transmitan Sebelum Purifikasi
Replikasi
Perlakuan
P1 P2 P3
Panjang Gelombang
(nm)
Persen Transmitan
(%)
Panjang Gelombang
(nm)
Persen Transmitan
(%)
Panjang Gelombang
(nm)
Persen Transmitan
(%)
1 436 92,148 426 94,737 422 96,326
2 426,5 95,9 420 95,531 426 95,09
3 419 99,785 424 99,817 427 96,895
Rata-Rata 427 95,94 423 96,69 425 96,1
SD 8,52 3,82 3,06 2,73 2,65 0,92
CV (%) 1,99 3,98 0,72 2,83 0,62 0,96
Lampiran 4. Panjang Gelombang dan Persen Transmitan Sesudah Purifikasi
Replikasi
Perlakuan
P1 P2 P3
Panjang Gelombang
(nm)
Persen Transmitan
(%)
Panjang Gelombang
(nm)
Persen Transmitan
(%)
Panjang Gelombang
(nm)
Persen Transmitan
(%)
1 438 95,415 432 97,04 422 96,753
2 432 96,603 427,5 96,822 426 96,417
3 426 99,969 428 99,893 427 96,971
Rata-Rata 432 97,33 429 97,92 432 97,71
SD 6 2,36 2,47 1,71 2,02 1,96
CV (%) 1,39 2,43 0,58 1,75 0,47 2
55
Lampiran 5. Grafik Panjang Gelombang Kontrol
Nama Gambar Grafik
Ekstrak Teh Hijau
Larutan Perak Nitrat
nm.
200.00 400.00 600.00 800.00
Abs.
4.400 4.000
3.000
2.000
1.000
0.000 -0.400
nm.
200.00 400.00 600.00 800.00
Abs.
3.408
3.000
2.000
1.000
0.000 -0.310
56
Lampiran 6. Grafik Panjang Gelombang Sebelum Purifikasi
Perlakuan Gambar Grafik
P1
P2
P3
nm.
400.00 600.00 800.00
Abs.
0.692
0.314
-0.063
nm.
400.00 600.00 800.00
Abs.
0.464
0.210
-0.044
nm.
400.00 600.00 800.00
Abs.
0.452
0.206
-0.041
57
Lampiran 7. Grafik Panjang Gelombang Sesudah Purifikasi
Perlakuan Gambar Grafik
P1
P2
P3
nm.
400.00 600.00 800.00
Abs.
0.599
0.272
-0.055
nm.
400.00 600.00 800.00
Abs.
0.401
0.182
-0.037
nm.
400.00 600.00 800.00
Abs.
0.393
0.178
-0.036
58
Lampiran 8. Grafik Persen Transmitan Sebelum Purifikasi
Perlakuan Gambar Grafik
P1
P2
P3
nm.
400.00 600.00 800.00
T%
100.814
96.087
91.360
nm.
400.00 600.00 800.00
T%
100.344
98.419
96.495
nm.
400.00 600.00 800.00
T%
100.372
98.215
96.058
59
Lampiran 9. Grafik Persen Transmitan Sesudah Purifikasi
Perlakuan Gambar Grafik
P1
P2
P3
nm.
400.00 600.00 800.00
T%
100.418
97.689
94.960
nm.
400.00 600.00 800.00
T%
100.344
98.419
96.495
nm.
400.00 600.00 800.00
T%
100.372
98.215
96.058
60
Lampiran 10. Hasil Ukuran Partikel dan PI Larutan Sintesis Nanosilver
61
Lampiran 11. Hasil Zeta Potensial Larutan Sintesis Nanosilver
62
Lampiran 12. Ethical Clearance
63
Lampiran 13. Hasil Analisis Paired T-Test
64
Lampiran 14. Hasil ANOVA: Single Factor Test
65
Lampiran 15. Perhitungan Konsentrasi Nanosilver Perhitungan Molaritas Infusa Teh Hijau
M = g
Mrx1000 V M = 0,2 g
458,37𝑥1000 mL 100 mL
M = 2
458,37
M = 0,00434632873006 M = 4,363 mM
Perhitungan Molaritas Campuran (Infusa Teh Hijau + AgNO3) Vc x Mc = V1. M1 + V2. M2
Mc =V1. M1 + V2. M2 Vc
Mc =9 mL . (4,363 mM) + 81mL . (1mM) 90 mL
Mc =120,267 90 Mc = 1,3363 mM = 0,0013363 M
Perhitungan Konsentrasi Nanosilver M = g
Mrx1000 V
0,0013363 M = x (g)
107,87x1000 mL 90 mL 0,0013363 M = 9x (g)
1,0787 9x = 0,0014416681 X = 0,0001601629788 g = 0,16 mg/mL
= 0,032 mg/0,2 mL atau 0,032 mg/semprot