BAB V. KESIMPULAN DAN SARAN
B. Saran
Perlu dilakukan penelitian lebih lanjut tentang :
1. Uji efek hepatoprotektif ekstrak metanol-air daun M. tanarius pada tikus jantan terinduksi galaktosamin.
2. Formulasi sediaan daun M. tanarius sebagai alternatif pengobatan penyakit hati.
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LAMPIRAN
Lampiran 1. Foto daun M. tanarius
Lampiran 2. Foto ekstrak metanol-air daun M. tanarius
Lampiran 5. Surat pengesahan Medical and Health Research Ethics Committee (MHREC)
Lampiran 6. Analisis statistik aktivitas serum ALT pada uji pendahuluan penentuan dosis hepatotoksin karbon tetraklorida dosis 2 ml/kgBB NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
ALT 15 1.4053E2 84.31901 51.00 311.00
One-Sample Kolmogorov-Smirnov Test
ALT
N 15
Normal Parametersa Mean 1.4053E2
Std. Deviation 8.43190E1
Most Extreme Differences Absolute .204
Positive .204
Negative -.157
Kolmogorov-Smirnov Z .790
Asymp. Sig. (2-tailed) .561
a. Test distribution is Normal.
Oneway Descriptives ALT N Mean Std. Deviation Std. Error
95% Confidence Interval for Mean
Minimum Maximum Lower Bound Upper Bound
CCl4 Dosis 2 ml/kgBB jam ke 0 5 73.2000 28.89983 12.92440 37.3161 109.0839 51.00 123.00 CCl4 Dosis 2 ml/kgBB jam ke 24 5 2.4640E2 38.02368 17.00471 199.1874 293.6126 219.00 311.00 CCl4 Dosis 2 ml/kgBB jam ke 48 5 1.0200E2 32.71085 14.62874 61.3841 142.6159 68.00 139.00 Total 15 1.4053E2 84.31901 21.77107 93.8390 187.2276 51.00 311.00
Test of Homogeneity of Variances
ALT
Levene Statistic df1 df2 Sig.
.296 2 12 .749
ANOVA
ALT
Sum of Squares df Mean Square F Sig.
Between Groups 86131.733 2 43065.867 38.555 .000
Within Groups 13404.000 12 1117.000
Total 99535.733 14
Post Hoc Tests
Multiple Comparisons
ALT Scheffe
(I) Orientasi_CCl4 (J) Orientasi_CCl4
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
CCl4 Dosis 2 ml/kgBB jam ke 0 CCl4 Dosis 2 ml/kgBB jam ke 24 -173.20000* 21.13764 .000 -232.1228 -114.2772 CCl4 Dosis 2 ml/kgBB jam ke 48 -28.80000 21.13764 .422 -87.7228 30.1228 CCl4 Dosis 2 ml/kgBB jam ke 24 CCl4 Dosis 2 ml/kgBB jam ke 0 173.20000* 21.13764 .000 114.2772 232.1228 CCl4 Dosis 2 ml/kgBB jam ke 48 144.40000* 21.13764 .000 85.4772 203.3228 CCl4 Dosis 2 ml/kgBB jam ke 48 CCl4 Dosis 2 ml/kgBB jam ke 0 28.80000 21.13764 .422 -30.1228 87.7228 CCl4 Dosis 2 ml/kgBB jam ke 24 -144.40000* 21.13764 .000 -203.3228 -85.4772
*. The mean difference is significant at the 0.05 level.
Homogeneous Subsets ALT
Scheffe
Orientasi_CCl4 N
Subset for alpha = 0.05
1 2
CCl4 Dosis 2 ml/kgBB jam ke 48 5 102.0000
CCl4 Dosis 2 ml/kgBB jam ke 24 5 246.4000
Sig. .422 1.000
Means for groups in homogeneous subsets are displayed.
Means
Case Processing Summary
Cases
Included Excluded Total
N Percent N Percent N Percent
ALT * Orientasi_CCl4 15 100.0% 0 .0% 15 100.0%
Report
ALT
Orientasi_CCl4 Mean N Std. Deviation
CCl4 Dosis 2 ml/kgBB jam ke 0 73.2000 5 28.89983
CCl4 Dosis 2 ml/kgBB jam ke 24 2.4640E2 5 38.02368
CCl4 Dosis 2 ml/kgBB jam ke 48 1.0200E2 5 32.71085
Total 1.4053E2 15 84.31901
ANOVA Table
Sum of Squares df Mean Square F Sig.
ALT * Orientasi_CCl4
Between Groups (Combined) 86131.733 2 43065.867 38.555 .000
Linearity 2073.600 1 2073.600 1.856 .198
Deviation from Linearity 84058.133 1 84058.133 75.253 .000
Within Groups 13404.000 12 1117.000
Total 99535.733 14
Measures of Association
R R Squared Eta Eta Squared
Lampiran 7. Analisis statistik aktivitas serum AST pada uji pendahuluan penentuan dosis hepatotoksin karbontetraklorida dosis 2 ml/kgBB NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 15 3.1200E2 211.52575 114.00 669.00
One-Sample Kolmogorov-Smirnov Test
AST
N 15
Normal Parametersa Mean 3.1200E2
Std. Deviation 2.11526E2
Most Extreme Differences Absolute .373
Positive .373
Negative -.175
Kolmogorov-Smirnov Z 1.446
Asymp. Sig. (2-tailed) .031
a. Test distribution is Normal.
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 15 3.1200E2 211.52575 114.00 669.00
Kruskal-Wallis Test
Ranks
Orientasi_CCl4 N Mean Rank
AST CCl4 Dosis 2 ml/kgBB jam ke 0 5 3.40
CCl4 Dosis 2 ml/kgBB jam ke 24 5 13.00
CCl4 Dosis 2 ml/kgBB jam ke 48 5 7.60
Total 15
Test Statisticsa,b
AST
Chi-Square 11.580
df 2
Asymp. Sig. .003
a. Kruskal Wallis Test
b. Grouping Variable: Orientasi_CCl4
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 15 3.1200E2 211.52575 114.00 669.00
Orientasi_CCl4 15 2.0000 .84515 1.00 3.00
Mann-Whitney Test
Ranks
Orientasi_CCl4 N Mean Rank Sum of Ranks
AST CCl4 Dosis 2 ml/kgBB jam ke 0 5 3.00 15.00
CCl4 Dosis 2 ml/kgBB jam ke 24 5 8.00 40.00 Total 10 Test Statisticsb AST Mann-Whitney U .000 Wilcoxon W 15.000 Z -2.611
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Orientasi_CCl4 NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 15 3.1200E2 211.52575 114.00 669.00
Orientasi_CCl4 15 2.0000 .84515 1.00 3.00
Mann-Whitney Test
Ranks
Orientasi_CCl4 N Mean Rank Sum of Ranks
AST CCl4 Dosis 2 ml/kgBB jam ke 0 5 3.40 17.00
CCl4 Dosis 2 ml/kgBB jam ke 48 5 7.60 38.00 Total 10 Test Statisticsb AST Mann-Whitney U 2.000 Wilcoxon W 17.000 Z -2.193
Asymp. Sig. (2-tailed) .028
Exact Sig. [2*(1-tailed Sig.)] .032a
a. Not corrected for ties.
b. Grouping Variable: Orientasi_CCl4
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 15 3.1200E2 211.52575 114.00 669.00
Orientasi_CCl4 15 2.0000 .84515 1.00 3.00
Mann-Whitney Test
Ranks
Orientasi_CCl4 N Mean Rank Sum of Ranks
CCl4 Dosis 2 ml/kgBB jam ke 48 5 3.00 15.00 Total 10 Test Statisticsb AST Mann-Whitney U .000 Wilcoxon W 15.000 Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Orientasi_CCl4
Means
Case Processing Summary
Cases
Included Excluded Total
N Percent N Percent N Percent
AST * Orientasi_CCl4 15 100.0% 0 .0% 15 100.0%
Report
AST
Orientasi_CCl4 Mean N Std. Deviation Std. Error of Mean
CCl4 Dosis 2 ml/kgBB jam ke 0 1.5120E2 5 31.91708 14.27375
CCl4 Dosis 2 ml/kgBB jam ke 24 5.9620E2 5 56.67186 25.34443
CCl4 Dosis 2 ml/kgBB jam ke 48 1.8860E2 5 7.30068 3.26497
Total 3.1200E2 15 211.52575 54.61571
ANOVA Table
Sum of Squares df Mean Square F Sig.
AST *
Orientasi_CCl4
Between Groups (Combined) 609269.200 2 304634.600 213.344 .000
Linearity 3496.900 1 3496.900 2.449 .144
Deviation from Linearity 605772.300 1 605772.300 424.240 .000
Within Groups 17134.800 12 1427.900
Measures of Association
R R Squared Eta Eta Squared
AST * Orientasi_CCl4 .075 .006 .986 .973
Lampiran 8. Analisis statistik aktivitas serum ALT perlakuan ekstrak metanol air daun M. tanarius setelah induksi karbon tetraklorida dosis 2
ml/kgBB NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
ALT 30 1.3607E2 63.29075 62.00 311.00
One-Sample Kolmogorov-Smirnov Test
ALT
N 30
Normal Parametersa Mean 1.3607E2
Std. Deviation 6.32908E1
Most Extreme Differences Absolute .216
Positive .216
Negative -.142
Kolmogorov-Smirnov Z 1.181
Asymp. Sig. (2-tailed) .123
Oneway Descriptives ALT N Mean Std. Deviation Std. Error
95% Confidence Interval for Mean
Minimum Maximum Lower Bound Upper Bound
Kontrol Hepatotoksin CCl4
Dosis 2 ml/kgBB 5 2.4640E2 38.02368 17.00471 199.1874 293.6126 219.00 311.00
Kontrol Negatif Olive Oil Dosis
2 ml/kgBB 5 82.2000 6.09918 2.72764 74.6269 89.7731 77.00 92.00
Kontrol Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB
5 82.4000 12.05404 5.39073 67.4329 97.3671 62.00 91.00
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB 5 1.7380E2 24.32488 10.87842 143.5967 204.0033 153.00 215.00
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB 5 1.3900E2 13.13393 5.87367 122.6921 155.3079 128.00 157.00
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 5 92.6000 7.26636 3.24962 83.5776 101.6224 82.00 100.00
Total 30 1.3607E2 63.29075 11.55526 112.4335 159.6998 62.00 311.00
Test of Homogeneity of Variances
ALT
Levene Statistic df1 df2 Sig.
2.367 5 24 .070
ANOVA
ALT
Sum of Squares df Mean Square F Sig.
Between Groups 106384.667 5 21276.933 52.207 .000
Within Groups 9781.200 24 407.550
Total 116165.867 29
Post Hoc Tests
Multiple Comparisons
ALT Scheffe
Difference (I-J) Lower Bound Upper Bound
Kontrol Hepatotoksin CCl4 Dosis 2 ml/kgBB
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 164.20000
* 12.76793 .000 117.9821 210.4179
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 164.00000
*
12.76793 .000 117.7821 210.2179
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB 72.60000
* 12.76793 .001 26.3821 118.8179
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB 107.40000
*
12.76793 .000 61.1821 153.6179
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 153.80000
* 12.76793 .000 107.5821 200.0179
Kontrol Negatif Olive Oil Dosis 2 ml/kgBB
Kontrol Hepatotoksin CCl4
Dosis 2 ml/kgBB -164.20000
*
12.76793 .000 -210.4179 -117.9821
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB -.20000 12.76793 1.000 -46.4179 46.0179
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -91.60000
*
12.76793 .000 -137.8179 -45.3821
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB -56.80000
*
12.76793 .009 -103.0179 -10.5821
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB -10.40000 12.76793 .983 -56.6179 35.8179
Kontrol Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB
Kontrol Hepatotoksin CCl4
Dosis 2 ml/kgBB -164.00000
*
12.76793 .000 -210.2179 -117.7821
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB .20000 12.76793 1.000 -46.0179 46.4179
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -91.40000
*
12.76793 .000 -137.6179 -45.1821
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB -56.60000
*
12.76793 .010 -102.8179 -10.3821
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB -10.20000 12.76793 .985 -56.4179 36.0179
Ekstrak Daun M.tanarius Dosis Rendah 0,426 g/kgBB Kontrol Hepatotoksin CCl4 Dosis 2 ml/kgBB -72.60000 * 12.76793 .001 -118.8179 -26.3821
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 91.60000
*
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 91.40000
* 12.76793 .000 45.1821 137.6179
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB 34.80000 12.76793 .231 -11.4179 81.0179
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 81.20000
* 12.76793 .000 34.9821 127.4179
Ekstrak Daun M.tanarius Dosis Tengah 1,280 g/kgBB
Kontrol Hepatotoksin CCl4
Dosis 2 ml/kgBB -107.40000
* 12.76793 .000 -153.6179 -61.1821
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 56.80000
* 12.76793 .009 10.5821 103.0179
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 56.60000
* 12.76793 .010 10.3821 102.8179
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -34.80000 12.76793 .231 -81.0179 11.4179
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 46.40000
* 12.76793 .049 .1821 92.6179
Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB
Kontrol Hepatotoksin CCl4
Dosis 2 ml/kgBB -153.80000
* 12.76793 .000 -200.0179 -107.5821
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 10.40000 12.76793 .983 -35.8179 56.6179
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 10.20000 12.76793 .985 -36.0179 56.4179
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -81.20000
* 12.76793 .000 -127.4179 -34.9821
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB -46.40000
* 12.76793 .049 -92.6179 -.1821
*. The mean difference is significant at the 0.05 level. Homogeneous Subsets
ALT
Scheffe
Kelompok_Perlakuan N
Subset for alpha = 0.05
1 2 3
Kontrol Negatif Olive Oil Dosis 2 ml/kgBB 5 82.2000
Kontrol Ekstrak Daun M.tanarius Dosis Tinggi 3,840
Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB 5 92.6000
Ekstrak Daun M.tanarius Dosis Tengah 1,280 g/kgBB 5 1.3900E2
Ekstrak Daun M.tanarius Dosis Rendah 0,426 g/kgBB 5 1.7380E2
Kontrol Hepatotoksin CCl4 Dosis 2 ml/kgBB 5 2.4640E2
Sig. .983 .231 1.000
Means for groups in homogeneous subsets are displayed.
Means
Case Processing Summary
Cases
Included Excluded Total
N Percent N Percent N Percent
ALT * Kelompok_Perlakuan 30 100.0% 0 .0% 30 100.0%
Report
ALT
Kelompok_Perlakuan Mean N Std. Deviation
Kontrol Hepatotoksin CCl4 Dosis 2 ml/kgBB 2.4640E2 5 38.02368
Kontrol Negatif Olive Oil Dosis 2 ml/kgBB 82.2000 5 6.09918
Kontrol Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB 82.4000 5 12.05404
Ekstrak Daun M.tanarius Dosis Rendah 0,426 g/kgBB 1.7380E2 5 24.32488
Ekstrak Daun M.tanarius Dosis Tengah 1,280 g/kgBB 1.3900E2 5 13.13393
Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB 92.6000 5 7.26636
Total 1.3607E2 30 63.29075
ANOVA Table
Sum of Squares df Mean Square F Sig.
ALT *
Kelompok_Perla kuan
Between Groups (Combined) 106384.667 5 21276.933 52.207 .000
Linearity 18375.131 1 18375.131 45.087 .000
Deviation from Linearity 88009.535 4 22002.384 53.987 .000
Within Groups 9781.200 24 407.550
Total 116165.867 29
Measures of Association
R R Squared Eta Eta Squared
Lampiran 9. Analisis statistik aktivitas serum AST perlakuan ekstrak metanol air daun M. tanarius setelah induksi karbon tetrakloridadosis 2
ml/kgBB NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 30 3.3557E2 195.75067 106.00 669.00
One-Sample Kolmogorov-Smirnov Test
AST
N 30
Normal Parametersa Mean 3.3557E2
Std. Deviation 1.95751E2
Most Extreme Differences Absolute .248
Positive .248
Negative -.171
Kolmogorov-Smirnov Z 1.356
Asymp. Sig. (2-tailed) .051
a. Test distribution is Normal.
Oneway
Descriptives
N Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
Minimum Maximum Lower Bound Upper Bound
Kontrol Hepatotoksin CCl4 Dosis 2
ml/kgBB 5 5.9620E2 56.67186 25.34443 525.8326 666.5674 522.00 669.00
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 5 1.1860E2 11.50217 5.14393 104.3182 132.8818 106.00 133.00
Kontrol Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 5 1.6260E2 14.36315 6.42339 144.7658 180.4342 139.00 175.00
Ekstrak Daun M.tanarius Dosis Rendah
0,426 g/kgBB 5 5.2160E2 24.46017 10.93892 491.2287 551.9713 499.00 560.00
Ekstrak Daun M.tanarius Dosis Tengah
1,280 g/kgBB 5 4.4280E2 18.45806 8.25470 419.8813 465.7187 422.00 462.00
Ekstrak Daun M.tanarius Dosis Tinggi
3,840 g/kgBB 5 1.7160E2 30.42696 13.60735 133.8199 209.3801 123.00 205.00
Total 30 3.3557E2 195.75067 35.73902 262.4722 408.6612 106.00 669.00
Test of Homogeneity of Variances
AST
Levene Statistic df1 df2 Sig.
3.232 5 24 .023
ANOVA
AST
Sum of Squares df Mean Square F Sig.
Between Groups 1089570.967 5 217914.193 241.452 .000
Within Groups 21660.400 24 902.517
Total 1111231.367 29
Post Hoc Tests
Multiple Comparisons
AST Tamhane
(I) Kelompok_Perlakuan (J) Kelompok_Perlakuan
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
Kontrol Hepatotoksin CCl4 Dosis 2 ml/kgBB
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 477.60000
*
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 433.60000
*
26.14575 .000 286.6472 580.5528
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB 74.60000 27.60435 .450 -61.8739 211.0739
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB 153.40000
* 26.65483 .036 11.1249 295.6751
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 424.60000
* 28.76630 .000 291.6271 557.5729
Kontrol Negatif Olive Oil Dosis 2 ml/kgBB
Kontrol Hepatotoksin CCl4 Dosis
2 ml/kgBB -477.60000
* 25.86117 .000 -627.7890 -327.4110
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB -44.00000
* 8.22922 .012 -78.3987 -9.6013
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -403.00000
* 12.08801 .000 -461.2204 -344.7796
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB -324.20000
* 9.72625 .000 -367.2227 -281.1773
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB -53.00000 14.54716 .194 -127.7243 21.7243
Kontrol Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB
Kontrol Hepatotoksin CCl4 Dosis
2 ml/kgBB -433.60000
* 26.14575 .000 -580.5528 -286.6472
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 44.00000
* 8.22922 .012 9.6013 78.3987
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -359.00000
* 12.68542 .000 -416.0758 -301.9242
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB -280.20000
* 10.45945 .000 -324.1320 -236.2680
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB -9.00000 15.04726 1.000 -81.3871 63.3871
Ekstrak Daun M.tanarius Dosis Rendah 0,426 g/kgBB
Kontrol Hepatotoksin CCl4 Dosis
2 ml/kgBB -74.60000 27.60435 .450 -211.0739 61.8739
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 403.00000
*
12.08801 .000 344.7796 461.2204
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 359.00000
*
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB 78.80000
* 13.70401 .008 20.9114 136.6886
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 350.00000
* 17.45910 .000 277.0638 422.9362
Ekstrak Daun M.tanarius Dosis Tengah 1,280 g/kgBB
Kontrol Hepatotoksin CCl4 Dosis
2 ml/kgBB -153.40000
* 26.65483 .036 -295.6751 -11.1249
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 324.20000
* 9.72625 .000 281.1773 367.2227
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 280.20000
* 10.45945 .000 236.2680 324.1320
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -78.80000
* 13.70401 .008 -136.6886 -20.9114
Ekstrak Daun M.tanarius Dosis
Tinggi 3,840 g/kgBB 271.20000
* 15.91540 .000 200.2645 342.1355
Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB
Kontrol Hepatotoksin CCl4 Dosis
2 ml/kgBB -424.60000
* 28.76630 .000 -557.5729 -291.6271
Kontrol Negatif Olive Oil Dosis 2
ml/kgBB 53.00000 14.54716 .194 -21.7243 127.7243
Kontrol Ekstrak Daun M.tanarius
Dosis Tinggi 3,840 g/kgBB 9.00000 15.04726 1.000 -63.3871 81.3871
Ekstrak Daun M.tanarius Dosis
Rendah 0,426 g/kgBB -350.00000
* 17.45910 .000 -422.9362 -277.0638
Ekstrak Daun M.tanarius Dosis
Tengah 1,280 g/kgBB -271.20000
* 15.91540 .000 -342.1355 -200.2645
*. The mean difference is significant at the 0.05 level. Means
Case Processing Summary
Cases
Included Excluded Total
N Percent N Percent N Percent
AST * Kelompok_Perlakuan 30 100.0% 0 .0% 30 100.0%
Report
AST
Kontrol Hepatotoksin CCl4 Dosis 2 ml/kgBB 5.9620E2 5 56.67186
Kontrol Negatif Olive Oil Dosis 2 ml/kgBB 1.1860E2 5 11.50217
Kontrol Ekstrak Daun M.tanarius Dosis Tinggi 3,840
g/kgBB 1.6260E2 5 14.36315
Ekstrak Daun M.tanarius Dosis Rendah 0,426 g/kgBB 5.2160E2 5 24.46017
Ekstrak Daun M.tanarius Dosis Tengah 1,280 g/kgBB 4.4280E2 5 18.45806
Ekstrak Daun M.tanarius Dosis Tinggi 3,840 g/kgBB 1.7160E2 5 30.42696
Total 3.3557E2 30 195.75067
ANOVA Table
Sum of Squares df Mean Square F Sig.
AST * Kelompok_ Perlakuan
Between Groups (Combined) 1089570.967 5 217914.193 241.452 .000
Linearity 44736.711 1 44736.711 49.569 .000
Deviation from Linearity 1044834.255 4 261208.564 289.422 .000
Within Groups 21660.400 24 902.517
Total 1111231.367 29
Measures of Association
R R Squared Eta Eta Squared
Lampiran 10. Analisis statistik aktivitas serum ALT dan serum AST perlakuan kontrol negatif olive oildosis 2 ml/kgBB
ALT
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
ALT 10 86.2000 9.37846 77.00 108.00
One-Sample Kolmogorov-Smirnov Test
ALT
N 10
Normal Parametersa Mean 86.2000
Std. Deviation 9.37846
Most Extreme Differences Absolute .193
Positive .193
Negative -.163
Kolmogorov-Smirnov Z .609
Asymp. Sig. (2-tailed) .851
a. Test distribution is Normal.
Means
Report
ALT
Kelompok_Perlakuan Mean N Std. Deviation Std. Error of Mean
Kontrol negatif olive oil 2 ml/kgBB jam ke 0 90.2000 5 10.98636 4.91325
Kontrol negatif olive oil 2 ml/kgBB jam ke
24 82.2000 5 6.09918 2.72764
Total 86.2000 10 9.37846 2.96573
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
ALT 10 86.2000 9.37846 77.00 108.00
Kruskal-Wallis Test
Ranks
Kelompok_Perlakuan N Mean Rank
ALT Kontrol negatif olive oil 2
ml/kgBB jam ke 0 5 6.70
Kontrol negatif olive oil 2
ml/kgBB jam ke 24 5 4.30
Total 10
Test Statisticsa,b
ALT
Chi-Square 1.580
df 1
Asymp. Sig. .209
a. Kruskal Wallis Test
b. Grouping Variable: Kelompok_Perlakuan
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
ALT 10 86.2000 9.37846 77.00 108.00
Kelompok_Perlakuan 10 1.50 .527 1 2
Mann-Whitney Test
Ranks
Kelompok_Perlakuan N Mean Rank Sum of Ranks
ALT Kontrol negatif olive oil 2
ml/kgBB jam ke 0 5 6.70 33.50
Kontrol negatif olive oil 2
ml/kgBB jam ke 24 5 4.30 21.50
Total 10
Test Statisticsb
ALT
Wilcoxon W 21.500
Z -1.257
Asymp. Sig. (2-tailed) .209
Exact Sig. [2*(1-tailed Sig.)] .222a
a. Not corrected for ties.
b. Grouping Variable: Kelompok_Perlakuan
AST
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 10 1.2070E2 11.65285 105.00 137.00
One-Sample Kolmogorov-Smirnov Test
AST
N 10
Normal Parametersa Mean 1.2070E2
Std. Deviation 1.16528E1
Most Extreme Differences Absolute .206
Positive .142
Negative -.206
Kolmogorov-Smirnov Z .650
Asymp. Sig. (2-tailed) .792
Means
Report
AST
Kelompok_Perlakuan Mean N Std. Deviation Std. Error of Mean
Kontrol negatif olive oil 2 ml/kgBB jam ke 0 1.2280E2 5 12.73578 5.69561
Kontrol negatif olive oil 2 ml/kgBB jam ke 24 1.1860E2 5 11.50217 5.14393
Total 1.2070E2 10 11.65285 3.68495
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 10 1.2070E2 11.65285 105.00 137.00
Kelompok_Perlakuan 10 1.5000 .52705 1.00 2.00
Kruskal-Wallis Test
Ranks
Kelompok_Perlakuan N Mean Rank
AST Kontrol negatif olive oil 2
ml/kgBB jam ke 0 5 5.90
Kontrol negatif olive oil 2
ml/kgBB jam ke 24 5 5.10
Total 10
Test Statisticsa,b
AST
Chi-Square .176
df 1
Asymp. Sig. .675
a. Kruskal Wallis Test
b. Grouping Variable: Kelompok_Perlakuan
NPar Tests
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
AST 10 1.2070E2 11.65285 105.00 137.00
Kelompok_Perlakuan 10 1.5000 .52705 1.00 2.00
Mann-Whitney Test
Ranks
Kelompok_Perlakuan N Mean Rank Sum of Ranks
AST Kontrol negatif olive oil 2
ml/kgBB jam ke 0 5 5.90 29.50
Kontrol negatif olive oil 2
ml/kgBB jam ke 24 5 5.10 25.50 Total 10 Test Statisticsb AST Mann-Whitney U 10.500 Wilcoxon W 25.500 Z -.419
Asymp. Sig. (2-tailed) .675
Exact Sig. [2*(1-tailed Sig.)] .690a
a. Not corrected for ties.
Lampiran 11. Perhitungan efek hepatoprotektif
Rumus perhitungan efek hepatoprotektif :
x 100 %
Maka perhitungan efek hepatoprotektif ALT serum sebagai berikut :
Kelompok perlakuan M. tanarius dosis 0,426 g/kg BB (po) + induksi karbon tetraklorida :
29,5%
Kelompok perlakuan M. tanarius dosis 1,280 g/kg BB (po) + induksi karbon tetraklorida :
43,6%
Kelompok perlakuan M. tanarius dosis 3,840 g/kg BB (po) + induksi karbon tetraklorida :
62,4%
Lampiran 12. Perhitungan penetapan peringkat dosis ekstrak metanol-air daun M. tanarius kelompok perlakuan
Dasar penetapan peringkat dosis:
Bobot tertinggi tikus = 250 g
Konsentrasi ekstrak metanol daun Macaranga tanarius yang dapat disedot dan dikeluarkan lewat spuit peroral = 38,4 % atau 384 mg/ml
Pemberian cairan secara per oral maksimal 5 ml, digunakan separuhnya saja menjadi 2,5 ml
Dengan dasar tersebut maka ditetapkan dosis tertinggi V x C = BB x D
Volume Pemberian x Konsentrasi = Berat badan x Dosis 2,5 ml x 384 mg/ml = 250 g BB x Dosis
Dosis = 3,84 mg/gBB
Untuk dua peringkat dosis di bawahnya, dosis tertinggi ini dibagi 3 kemudian
