5 KESIMPULAN DAN SARAN
5.2 Saran
Pada kegiatan transgenesis rumput laut Kappaphycus alvarezii disarankan menggunakan promoter CMV, dan pengembangan metode induksi diferensiasi embrio somatik K. alvarezii menjadi rumput laut muda perlu dilakukan secara intensif untuk mendukung aplikasi transgenesis dalam produksi bibit rumput laut unggul.
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Lampiran 1 Skema prosedur sterilisasi air laut
stok air laut 30 ‰
difilter dengan membran filter 0,2 µm
diautoclave pada 121oC 15 menit
kontainer stok air laut steril
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Lampiran 2 Media kultur conway (Liao et al. 1983)
Komponen kimia Dosis Satuan Stok A
Fe Cl2 . 6H2O 1,30 gram MnCl2 . 4H2O 0,36 gram
H3BO3 33,60 gram
EDTA (Na-salt) 45,00 gram
NaH2PO4 . 2H2O 20,00 gram NaNO3 100,00 gram Akuades 1000,00 ml Stok B ZnCl2 2,10 gram CoCl2 . 6H2O 2,00 gram (NH4)6 . Mo6O24 . 4H2O 0,90 gram CuSO4 . 5H2O 2,00 gram Akuades 100,00 ml Stok C Vitamin B12 10,00 mg Vitamin B1 200,00 mg Akuades 100,00 ml Stok D Na2SiO3 . 5H2O 4,00 gram Akuades 100,00 ml
Catatan : 1. Stok A dipanaskan sampai mendidih, tambahkan 1 ml Stok B 2. Larutan Conway ditambahkan 2 ml per liter air laut steril dan vitamin 1 tetes (500 μl)
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Lampiran 3 Elemen-elemen faktor transkripsi promoter CMV (cytomegalovirus) (lanjutan…)
Lampiran 4 Elemen-elemen faktor transkripsi promoter CaMV (cauliflower mozaicvirus)