BAB I PENDAHULUAN
BAB 5 KESIMPULAN DAN SARAN
5.2 Saran
1. Dilakukan penelitian lebih lanjut mengenai analisis kadar dari thymoquinone pada formulasi emulsi MBJH.
2. Dilakukan penelitian lebih lanjut mengenai penambahan antioksidan dalam sediaan emulsi MBJH.
3. Dilakukan penelitian lebih lanjut mengenai penggunaan kombinasi antara emulgator alam dan sintetik.
4. Dilakukan penelitian lebih lanjut mengenai uji aktivitas antioksidan pada emulsi MBJH.
45
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51 Lampiran 1. Kerangka Penelitian
Pembuatan emulsi dengan kondisi optimasi Pembuatan emulsi MBJH Optimasi homogenisasi Evaluasi fisik emulsi MBJH Analisis komponen MBJH sebelum dan setelah
penyimpanan
Organoleptis Uji pH Uji viskositas
Pengukuran diameter Rata-rata globul Uji sentrifugasi Optimasi GCMS Analisis komponen senyawa MBJH sebelum dan setelah
penyimpanan
Preparasi
sampel uji Demulsifikasi Ekstraksi
Analisis komponen senyawa MBJH
UIN Syarif Hidayatullah Jakarta Lampiran 2. Perhitungan Penimbangan Bahan
A. Emulsi A
1. Minyak biji jinten hitam
2. Tragakan 3. Sukrosa 4. Na benzoat 5. Aquades = 500 – (50 + 7,5 + 125 + 0,5) gram = 317 gram Mendispersikan Tragakan = 20 x 7,5 = 150 g Melarutkan Sukrosa = 0,5 x 50 = 62,5 g ~ 63 g Melarutkan Na benzoat = 1,8 x 0,5 = 0,9 g ~ 1 g Sisa Aquades = 317 – (150 + 62,5 + 0,9) g = 103,6 ~ 104 g
UIN Syarif Hidayatullah Jakarta B. Emulsi B
1. Minyak biji jinten hitam
2. Tragakan 3. Sukrosa 4. Na benzoat 5. Aquades = 500 – (50 + 7,5 + 125 + 0,5) gram = 317 gram Mendispersikan Tragakan = 20 x 7,5 = 150 g Melarutkan Sukrosa = 0,5 x 50 = 62,5 g ~ 63 g Melarutkan Na benzoat = 1,8 x 0,5 = 0,9 g ~ 1 g Sisa Aquades = 317 – (150 + 62,5 + 0,9) g = 103,6 ~ 104 g
UIN Syarif Hidayatullah Jakarta Lampiran 3. Pehitungan Diameter Rata-rata Globul Emulsi MBJH Sebelum
dan Setelah Penyimpanan A. Diameter Globul Rata-rata Hari ke-0
Emulsi A
Ukuran Diameter Rata-rata Globul
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 10 24,5 4,0-6,9 5,45 14 76,3 7,0-9,9 8,45 46 388,7 10,0-12,9 11,45 304 3480,8 13,0-15,9 14,45 82 1184,9 16,0-18,9 17,45 47 820,15 19,0-21,9 20,45 39 797,55 22,0-24,9 23,45 120 2814 25,0-27,9 26,45 15 396,75 28,0-30,9 29,45 8 235,6 31-33,9 32,45 3 97,35 34,0-36,9 35,45 1 35,45 37,0-39,9 38,45 0 0 n = 689 nd = 10352,05
UIN Syarif Hidayatullah Jakarta Emulsi B
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 8 19,6 4,0-6,9 5,45 22 119,9 7,0-9,9 8,45 57 481,65 10,0-12,9 11,45 217 2824,65 13,0-15,9 14,45 101 1459,45 16,0-18,9 17,45 50 872,5 19,0-21,9 20,45 30 613,5 22,0-24,9 23,45 150 3517,5 25,0-27,9 26,45 6 158,7 28,0-30,9 29,45 6 176,7 31-33,9 32,45 5 162,25 34,0-36,9 35,45 0 0 37,0-39,9 38,45 0 0 n = 652 nd = 10406,4 Ukuran Diameter Rata-rata Globul
UIN Syarif Hidayatullah Jakarta B. Diameter Globul Rata-rata Hari ke-2
Emulsi A
Ukuran Diameter Rata-rata Globul
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 8 19,6 4,0-6,9 5,45 64 348,8 7,0-9,9 8,45 50 422,5 10,0-12,9 11,45 121 1385,45 13,0-15,9 14,45 129 1864,05 16,0-18,9 17,45 30 523,5 19,0-21,9 20,45 170 3476,5 22,0-24,9 23,45 30 703,5 25,0-27,9 26,45 27 714,15 28,0-30,9 29,45 23 677,35 31-33,9 32,45 15 486,75 34,0-36,9 35,45 3 106,35 37,0-39,9 38,45 2 76,9 n =672 nd = 10805,4
UIN Syarif Hidayatullah Jakarta Emulsi B
Ukuran Diameter Rata-rata Globul
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 5 12,25 4,0-6,9 5,45 50 272,5 7,0-9,9 8,45 70 591,5 10,0-12,9 11,45 143 1637,35 13,0-15,9 14,45 130 1878,5 16,0-18,9 17,45 23 401,35 19,0-21,9 20,45 27 552,15 22,0-24,9 23,45 33 773,85 25,0-27,9 26,45 17 449,65 28,0-30,9 29,45 45 1325,5 31-33,9 32,45 65 2109,25 34,0-36,9 35,45 8 283,6 37,0-39,9 38,45 7 269,15 n =630 nd = 10556,35
UIN Syarif Hidayatullah Jakarta C. Diameter Globul Rata-rata Hari ke-7
Emulsi A
Ukuran Diameter Rata-rata Globul
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 0 0 4,0-6,9 5,45 27 147,15 7,0-9,9 8,45 71 272,5 10,0-12,9 11,45 163 1866,35 13,0-15,9 14,45 89 1286,05 16,0-18,9 17,45 50 872,5 19,0-21,9 20,45 61 1247,45 22,0-24,9 23,45 21 492,45 25,0-27,9 26,45 10 264,5 28,0-30,9 29,45 80 2356 31-33,9 32,45 40 1298 34,0-36,9 35,45 26 921,7 37,0-39,9 38,45 11 422,95 n = 659 nd = 11447,6
UIN Syarif Hidayatullah Jakarta Emulsi B
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 2 4,9 4,0-6,9 5,45 63 343,35 7,0-9,9 8,45 66 557,7 10,0-12,9 11,45 49 561,05 13,0-15,9 14,45 127 1835,15 16,0-18,9 17,45 53 924,85 19,0-21,9 20,45 44 899,8 22,0-24,9 23,45 60 1407 25,0-27,9 26,45 29 767,05 28,0-30,9 29,45 71 2090,95 31-33,9 32,45 43 1395,35 34,0-36,9 35,45 14 496,3 37,0-39,9 38,45 9 346,05 n = 621 nd = 11457,45
UIN Syarif Hidayatullah Jakarta D. Diameter Globul Rata-rata Hari ke-14
Emulsi A
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 0 0 4,0-6,9 5,45 19 103,55 7,0-9,9 8,45 79 667,55 10,0-12,9 11,45 44 503,8 13,0-15,9 14,45 80 1156 16,0-18,9 17,45 50 827,5 19,0-21,9 20,45 73 1492,85 22,0-24,9 23,45 51 1195,95 25,0-27,9 26,45 40 1058 28,0-30,9 29,45 19 559,55 31-33,9 32,45 36 1168,2 34,0-36,9 35,45 60 2127 37,0-39,9 38,45 46 1768,7 n = 597 nd = 12673,65
UIN Syarif Hidayatullah Jakarta Emulsi B
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 0 0 4,0-6,9 5,45 2 10,9 7,0-9,9 8,45 51 430,95 10,0-12,9 11,45 63 721,35 13,0-15,9 14,45 102 1473,9 16,0-18,9 17,45 50 872,5 19,0-21,9 20,45 42 858,9 22,0-24,9 23,45 30 703,5 25,0-27,9 26,45 55 1454,75 28,0-30,9 29,45 73 2149,85 31-33,9 32,45 49 1590,05 34,0-36,9 35,45 17 602,65 37,0-39,9 38,45 41 1576,45 n = 573 nd = 12445,75
UIN Syarif Hidayatullah Jakarta E. Diameter Globul Rata-rata Hari ke-21
Emulsi A
Ukuran Diameter Rata-rata Globul
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 0 0 4,0-6,9 5,45 0 0 7,0-9,9 8,45 44 371,8 10,0-12,9 11,45 38 435,1 13,0-15,9 14,45 12 173,4 16,0-18,9 17,45 31 540,95 19,0-21,9 20,45 43 879,35 22,0-24,9 23,45 12 281,4 25,0-27,9 26,45 36 952,2 28,0-30,9 29,45 74 2179,3 31-33,9 32,45 99 3212,55 34,0-36,9 35,45 100 3545 37,0-39,9 38,45 67 2576,15 n = 556 nd = 15147,2
UIN Syarif Hidayatullah Jakarta Emulsi B
Rentang Nilai Tengah (d) Jumlah Globul (n) nd
1,0-3,9 2,45 0 0 4,0-6,9 5,45 0 0 7,0-9,9 8,45 0 0 10,0-12,9 11,45 89 1019,05 13,0-15,9 14,45 130 1878,5 16,0-18,9 17,45 0 0 19,0-21,9 20,45 1 20,45 22,0-24,9 23,45 0 0 25,0-27,9 26,45 0 0 28,0-30,9 29,45 25 736,25 31-33,9 32,45 170 5516,5 34,0-36,9 35,45 34 1205,3 37,0-39,9 38,45 70 2691,5 n = 519 nd = 13067,55
UIN Syarif Hidayatullah Jakarta Lampiran 4. Perhitungan Rendemen Hasil Ekstraksi Minyak Emulsi MBJH
Sebelum dan Setelah Penyimpanan A. Rendemen Hari ke- 0
Emulsi A Emulsi B
Emulsi : 20,0398 gram
HCl 36% : 4 ml
Aquades : 8 ml
n-Heksane : 3 x 15 ml
Berat vial kosong : 7,4932
Berat vial kosong + Minyak : 8,1663 gram
Minyak yang didapat :
8,1663 - 7,4932 = 0,6731gram Rendemen Minyak : Emulsi : 20,0411 gram HCl 36% : 4 ml Aquades : 8 ml n-Heksane : 3 x 15 ml
Berat vial kosong : 7,5886
Berat vial kosong + Minyak : 8,2221 gram
Minyak yang didapat : 8,2221- 7,5886 = 0,6335
UIN Syarif Hidayatullah Jakarta B. Rendemen Hari ke- 2
Emulsi A Emulsi B
Emulsi : 20,0339 gram
HCl 36% : 4 ml
Aquades : 8 ml
n-Heksane : 3 x 15 ml
Berat vial kosong : 7,3689 gram
Berat vial kosong + Minyak : 7,8746 gram
Minyak yang didapat : 7,8746 - 7,3689 = 0,5057 g Rendemen Minyak : Emulsi : 20,0333 gram HCl 36% : 4 ml Aquades : 8 ml n-Heksane : 3 x 15 ml
Berat vial kosong : 7,7199
Berat vial kosong + Minyak : 8,2221 gram
Minyak yang didapat : 8,2221 – 7,7199 = 0,5022
UIN Syarif Hidayatullah Jakarta C. Rendemen Hari ke- 7
Emulsi A Emulsi B
Emulsi : 20,0192 gram
HCl 36% : 4 ml
Aquades : 8 ml
n-Heksane : 3 x 15 ml
Berat vial kosong : 7,8903 gram
Berat vial kosong + Minyak : 8,3093 gram
Minyak yang didapat : 8,3093 - 7,8903 = 0,419 gram Rendemen Minyak : Emulsi : 20,0060 gram HCl 36% : 4 ml Aquades : 8 ml n-Heksane : 3 x 15 ml
Berat vial kosong : 8,2046 gram
Berat vial kosong + Minyak : 8,5770 gram
Minyak yang didapat : 8,5770 - 8,2046 = 0,3724 gram
UIN Syarif Hidayatullah Jakarta D. Rendemen Hari ke- 14
Emulsi A Emulsi B
Emulsi : 20,0109 gram
HCl 36% : 4 ml
Aquades : 8 ml
n-Heksane : 3 x 15 ml
Berat vial kosong : 8,2741 gram
Berat vial kosong + Minyak : 8,5564 gram
Minyak yang didapat : 8,5564 - 8,2741 = 0,2823 Rendemen Minyak : Emulsi : 20,0357 gram HCl 36% : 4 ml Aquades : 8 ml n-Heksane : 3 x 15 ml
Berat vial kosong : 8,1101 gram
Berat vial kosong + Minyak : 8,4315 gram
Minyak yang didapat : 8,4315 - 8,1101 = 0,3214 gram
UIN Syarif Hidayatullah Jakarta E. Rendemen Hari ke- 21
Emulsi A Emulsi B
Emulsi : 20,0104 gram
HCl 36% : 4 ml
Aquades : 8 ml
n-Heksane : 3 x 15 ml
Berat vial kosong : 8,2601 gram
Berat vial kosong + Minyak : 8,4605 gram
Minyak yang didapat : 8,4605 - 8,2601 = 0,2004 gram Rendemen Minyak : Emulsi : 20,0126 gram HCl 36% : 4 ml Aquades : 8 ml n-Heksane : 3 x 15 ml
Berat vial kosong : 8,4550 gram
Berat vial kosong + Minyak : 8,7061 gram
Minyak yang didapat : 8,7061 - 8,4550 = 0,2511 gram
UIN Syarif Hidayatullah Jakarta Lampiran 5. Perhitungan Konsentrasi Minyak Hasil Ekstraksi Emulsi
MBJH
Sejumlah minyak yang telah diekstraksi ditimbang kemudian dilarutkan dengan pelarut heksan sebanyak 3 ml. Setelah itu divortex dan kemudian sampel disuntikkan ke GCMS. Perhitungan konsentrasi dari sampel yaitu:
A. Hari ke- 0 Emulsi A
Konsentrasi Emulsi B
UIN Syarif Hidayatullah Jakarta Lampiran 6. Hasil Kromatogram GCMS MBJH Sebelum dan Setelah
Penyimpanan A. Kromatogram MBJH Hari ke-0
UIN Syarif Hidayatullah Jakarta B. Kromatogram MBJH Hari ke-21
UIN Syarif Hidayatullah Jakarta C. Kromatogram MBJH Emulsi Hari ke- 0 (Emulsi A)
UIN Syarif Hidayatullah Jakarta D. Kromatogram MBJH Emulsi Hari ke- 0 (Emulsi B)
UIN Syarif Hidayatullah Jakarta E. Kromatogram MBJH Emulsi Hari ke- 2 (Emulsi A)
UIN Syarif Hidayatullah Jakarta F. Kromatogram MBJH Emulsi Hari ke- 2 (Emulsi B)
UIN Syarif Hidayatullah Jakarta G. Kromatogram MBJH Emulsi Hari ke- 7 (Emulsi A)
UIN Syarif Hidayatullah Jakarta H. Kromatogram MBJH Emulsi Hari ke- 7 (Emulsi B)
UIN Syarif Hidayatullah Jakarta I. Kromatogram MBJH Emulsi Hari ke- 14 (Emulsi A)
UIN Syarif Hidayatullah Jakarta J. Kromatogram MBJH Emulsi Hari ke- 14 (Emulsi B)
UIN Syarif Hidayatullah Jakarta K. Kromatogram MBJH Emulsi Hari ke- 21 (Emulsi A)
UIN Syarif Hidayatullah Jakarta L. Kromatogram MBJH Emulsi Hari ke- 21 (Emulsi B)
UIN Syarif Hidayatullah Jakarta Lampiran 7. Dokumentasi Alat, Bahan, dan Kegiatan Penelitian
MBJH Sukrosa Tragakan Na benzoat
Emulsi Stirer Homogenizer Viskometer pH Meter
UIN Syarif Hidayatullah Jakarta Lampiran 8. Sertifikat Analisis Minyak Biji Jinten Hitam
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