SEKOLAH PASCASARJANA INSTITUT PERTANIAN BOGOR

KELAPA SAWIT

SEKOLAH PASCASARJANA INSTITUT PERTANIAN BOGOR

BOGOR 2014

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Lampiran 1. Lokasi disain primer gen SAD spesifik (SAD 1 dan SAD 2) berdasarkan aksesi U68756 NCBI

GenBank: U68756.1

LOCUS EGU68756 1715 bp mRNA linear PLN 21-MAR-2000 DEFINITION Elaeis guineensis stearoyl-Acyl-carrier protein desaturase mRNA, partial cds.

ACCESSION U68756

VERSION U68756.1 GI:1785861 KEYWORDS .

SOURCE Elaeis guineensis (African oil palm) ORGANISM Elaeis guineensis

Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Arecaceae; Arecoideae; Cocoseae; Elaeidinae; Elaeis.

REFERENCE 1 (bases 1 to 1715) AUTHORS Shah,F.H. and Rashid,O.

TITLE Nucleotide sequence of a complementary DNA clone encoding stearoyl-Acyl-carrier protein (Accession No. U68756) from Elaeis guineensis var tenera (PGR96-110)

JOURNAL Plant Physiol. 112, 1399 (1996) REFERENCE 2 (bases 1 to 1715)

AUTHORS Shah,F.H. and Rashid,O. TITLE Direct Submission

JOURNAL Submitted (02-SEP-1996) Genetics, The National University of Malaysia, Bangi, Selangor 43600, Malaysia

FEATURES Location/Qualifiers source 1..1715 /organism="Elaeis guineensis" /mol_type="mRNA" /variety="tenera" /db_xref="taxon:51953" /tissue_type="mesocarp" CDS <1..1305 /EC_number="1.14.19.2" /codon_start=1

/product="stearoyl-Acyl-carrier protein desaturase" /protein_id="AAB41041.1" /db_xref="GI:1785862" /translation="GTRDKGGAFLSFSFFHSLPPLSLSPKGEKRKEERRRKEERA MASMVAFRPEAFLCFSPPKTTRSTRSPRISMASTVGPSTKVEIPKKPFMPPREVH VQVTHSMPPQKIEIFKSLEDWAENNILVHLKPVEKCWQPQDFLPDPSSEGFHEEV KELRERSKEIPDGYYVCLVGDMITEEALPTYQTMLNTLDGVRDETGASLTSWAVW TRAWTAEENRHGDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENSPYLGFI YTSFQERATFISHGNTARHAKEHGDVKLAQICGTIASDEKRHETAYTKIVEKLFE IDPDGTVLSFADMMKKKISMPAHLMYDGQDDNLFEHFSAVAQRLGVDTAKDYADI LEFLINRWKVGELTGFSGEGKRAQDFVCTLAPRIRRIEERAQERAKQAPRIPCSW IYGREVQL"

ORIGIN

1 ggcacgagag acaaaggagg ggcctttctc tctttctcct tctttcactc ccttcctcct 61 ctctctctct ctcctaaggg agaaaaaagg aaggaagaaa ggaggcgaaa agaagaaagg 121 gcaatggcgt cgatggtggc cttccgaccg gaggcgttct tatgcttctc tcctcccaag 181 accacgagga gtaccagatc tcccaggatt tccatggcct ccaccgttgg cccctccacc 241 aaggttgaga ttccaaaaaa gcccttcatg cctccgcgtg aggtacatgt tcaggtcaca 301 cattcaatgc cacctcagaa gattgagatt tttaagtcat tagaggactg ggcagaaaat 361 aatatcttgg tgcatttgaa gcctgttgag aagtgttggc agccccagga ttttctgcct 421 gatccatcat cagaaggatt tcatgaagag gttaaggaac tgagagaacg ctctaaggag 481 atccctgatg gttattatgt ttgcttggtt ggagatatga tcactgaaga agctcttcct 541 acatatcaaa caatgctcaa cacccttgat ggagttcgag atgagacagg ggcaagcctt 601 acttcttggg cagtctggac gagggcttgg actgctgaag agaacagaca tggggacctt 661 ctcaacaagt atctatacct ttctggtaga gtggacatga agcaaattga gaaaacaatc 721 caatatctga taggttctgg aatggatcct aggacagaga acagccccta ccttggtttt 781 atatacacct catttcagga gagggcgact ttcatttccc atgggaatac tgccaggcat 841 gccaaggaac atggggacgt gaagttggct cagatatgtg gtacaattgc ctcagatgag 901 aaacgccatg agacagctta tactaagatc gtggagaaac tgtttgagat cgacccggat 961 ggtacggtgc tttcctttgc tgacatgatg aagaagaaga tctcaatgcc tgcccatctg

1021 atgtatgatg gtcaggatga caacctcttc gagcacttct cggcagtagc ccagcgtttg 1081 ggtgtcgaca cagccaagga ctatgccgac atacttgagt tccttattaa taggtggaaa 1141 gtgggtgagt taaccggctt ctctggggaa ggtaagagag cccaggactt cgtctgcact 1201 cttgctccca ggatcaggag gattgaagaa agagctcagg aaagggccaa gcaagcacca 1261 cgcatacctt gcagctggat ctatggccgg gaagtgcaac tctgagcatg accaatatct 1321 gttttggttt aaataccagt tcttcagggt tggccaaaat tttgcatctg catgtatcag 1381 ctatatttat agggttttga aaacgattag cttaaaatcc atatatgctt tgttagtagg 1441 tcaggtcagg atggggaaag ccgtagatac tcttttaagc tatcacagat catcttatga 1501 gcaggtgagg gatgctgttg aagtggtgtc cagttggaaa ccttcctgtt tcaatgtttt 1561 ctttcgtgta ttagtactcc tatgtaggaa aatgggctga ttactgtatc tggaacctag

1621 ttcttgctcg cttttttcaa tgtcgtatgc ttcatctgga atgtctaaat ttcagtctat 1681 caatcttaag ataaaaaaaa aaaaaaaaaa ctcga

SAD 1 F

SAD 1 R SAD 2 F

Lampiran 2. Sequense Gen SAD dari 5 Sumber Keragaman Kelapa Sawit (1) Elaeis guineensis Var. Tenera (nigrescens) - Tenera-64

Exon, Intron ( intron yang di highlight) dan 3UTR’s, stop kodon gen SDA dengan garis bawah

CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA CTGATTGCTT CCATAATTTG TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATATGTCAC ATCAATTAAT ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA GATCTCAATG CCTGCCCA TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG TAGCCCAGCG TTTGGGTGTC TACACAGCCA AGGACTATGC CGACATACTT GAGTTCCTTA TTAATAGGTG GAAAGTGGGT GAGTTAACCG GCTTCTCTGG GGAAGGTAAG AGAGCCCAGG ACTTCGTCTG CACTCTTGCT CCCAGGATCA GGAGGATTGA AGAAAGAGCT CAGGAAAGGG CCAAGCAAGC ACCACGCATA CCTTTCAGCT GGATCTATGG CCGGGAAGTG CAACTCTGAG CATGACCAAT ATCTGTTTTG GTTTAAATAC CAGTTCTTCA GGGTTGGCCA AAATTTTGCA TCTGCATGTA TCAGCTATAT TTATAGGGTT TTGAAAACGA TTAGCTTAAA ATCCATATAT GCTTTGTTAG TAGGTCAGGT CAGGATGGGG AAAGCCGTAG ATACTCTTTT AAGCTATCAC AGATCATCTT ATGAGCAGGT GAGGGATGCT GTTGAAGTGG TGTCCAGTTG GAAACCTTCC TGTTTCAATG TTTTCTTTCG TGTATTAGTA CTCCTATGTA G

(2) Sequense Elaeis guineensis Var. Dura (nigrescens) - Dura-50

CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT GTTATCTTTT TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA GTGATTGCTT CCATAATTTG TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATATGTCAC ATCAATTAAT ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA GATCTCAATG CCTGCCCA TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG TAGCCCAGCG TTTGGGTGTC TACACAGCCA AGGACTATGC CGACATACTT GAGTTCCTTA TTAATAGGTG GAAAGTGGGT GAGTTAACCG GCTTCTCTGG GGAAGGTAAG AGAGCCCAGG ACTTCGTCTG CACTCTTGCT CCCAGGATCA GGAGGATTGA AGAAAGAGCT CAGGAAAGGG CCAAGCAAGC ACCACGCATA CCTTTCAGCT GGATCTATGG CCGGGAAGTG CAACTCTGAG CATGACCAAT ATCTGTTTTG GTTTAAATAC CAGTTCTTCA GGGTTGGCCA AAATTTTGCA TCTGCATGTA TCAGCTAT TTATAGGGTT TTGAAAACGA TTAGCTTAAA ATCCATATAT GCTTTGTTAG TAGGTCAGGT CAGGATGGGG AAAGCCGTAG ATACTCTTTT AAGCTATCAC AGATCATCTT ATGAGCAGGT GAGGGATGCT GTTGAAGTGG TGTCCAGTTG GAAACCTTCC TGTTTCAATG TTTTCTTTCG TGTATTAGTA CTCCTATGTA GGAAAATGGG CTGATTACTG TATCTGGAAC CTAGTTCTTG CTCG

(3) Sequense Elaeis guineensis Var. Pisifera (nigrescens) - Pisifera-46

CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC AATCATGGAG AAACTTCTCT GAGATACGGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATATCTTGC TTGATATTAA GTTATGCCTG AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA GTGATTGCTT CCATAATTTG TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATATGTCAC ATCAATTAAT ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTATGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA GATCTCAATG CCTGCCCA TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG TAGCCCAGCG TTTGGGTGTC TACACAGCCA AGGACTATGC CGACATACTT GAGTTCCTTA TTAATAGGTG GAAAGTGGGT GAGTTAACCG GCTTCTCTGG GGAAGGTAAG AGAGCCCAGG ACTTCGTCTG CACTCTTGCT CCCAGGATCA GGAGGATTGA AGAAAGAGCT CAGGAAAGGG CCAAGCAAGC ACCACGCATA CCTTTCAGCT GGATCTATGG CCGGGAAGTG CAACTCTGAG CATGACCAAT ATCTGTTTTG GTTTAAATAC CAGTTCTTCA GGGTTGGCCA AAATTTTGCA TCTGCATGTA TCAGCTAT TTATAGGGTT TTGAAAACGA TTAGCTTAAA ATCCATATAT GCTTTGTTAG TAGGTCAGGT CAGGATGGGG AAAGCCGTAG ATACTCTTTT AAGCTATCAC AGATCATCTT ATGAGCAGGT GAGGGATGCT GTTGAAGTGG TGTCCAGTTG GAAACCTTCC TGTTTCAATG TTTTCTTT

(4) Sequense Elaeis guineensis Var. Virescens - Virescens-34

CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA CTGATTGCTT CCATAATTTG TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATAGGTCAC ATCAATTAAT ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA GATCTCAATG CCTGCCCAAT TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG TAGCCCAGCG TTTGGGTGTC TACACAGCCA AGGACTATGC CGACATACTT GAGTTCCTTA TTAATAGGTG GAAAGTGGGT GAGTTAACCG GCTTCTCTGG GGAAGGTAAG AGAGCCCAGG ACTTTGTCTG CACTCTTGCT CCCAGGATCA GGAGGATTGA AGAAAGAGCT CAGGAAAGGG CCAAGCAAGC ACCACGCATA CCTTTCAGCT GGATCTATGG CCGGGAAGTG CAACTCTGAG CATGACCAAT ATCTGTTTTG GTTTAAATAC CAGTTCTTCA GGGTTGGCCA AAATTTTGCA TCTGCATGTA TCAGCTAT TTATAGGGTT TTGAAAACGA TTAGCTTAAA ATCCATATAT GCTTTGTTAG TAGGTCAGGT CAGGATGGGG AAAGCCGTAG ATACTCTTTT AAGCTATCAC AGATCATCTT ATGAGCAGGT GAGGGATGCT GTTGAAGTGG TGTCCAGTTG GAAACCTTCC TGTTTCAATG TTTTCTTTCG TGTATTAGTA CTCCTATGTA GGAAAATGGG CTGATTACTG TATCTGGAAC CTAGTTCTTG CTCGAGATGA CAACCTCTTC GAGCACTTCT CGGCAGTAGC CCAGCGTTTG GGTGTCTACA CAGCCAAGGA CTATGCCGAC ATACTTGAGT TCTTATTAAT AGGTGAAGTG GGTGATTTAC GGCTTCTCTG GGGAAGGTAG AACCAGGACT TTGTCTGCAC TCTTGCTCCA GGATCAGGAG GATGAAAAAG ATCAGGAAAG GCCAACAGCA CCACCTACCC TTCACTGGAT CAGGCCGGAA GGCACTTGGA CATGACAAAC TTGTTTGTTA ATCAGTCTCA GGTGCCATTG GCACGCATGA TACCTATTTA AGGATTGAGA TAGCTAATAC CAATGCTTGT GAACAGTCAG GATCGGATTG GGATATAGC

(5) Sequense Elaeis oleifera - E.oleifera 68

CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA GTGATTGCTT CCATAATTTG TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATAGGTCAC ATCAATTAAT ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA GATCTCAATG CCTGCCCA TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG TAGCCCAGCG TTTGGGTGTC TACACAGCCA AGGACTATGC CGACATACTT GAGTTCCTTA TTAATAGGTG GAAAGTGGGT GAGTTAACCG GCTTCTCTGG GGAAGGTAAG AGAGCCCAGG ACTTCGTCTG CACTCTTGCT CCCAGGATCA GGAGGATTGA AGAAAGAGCT CAGGAAAGGG CCAAGCAAGC ACCACGCATA CCTTTCAGCT GGATCTATGG CCGGGAAGTG CAACTCTGAG CATGACCAAT ATCTGTTTTG GTTTAAATAC CAGTTCTTCA GGGTTGGCCA AAATTTTGCA TCTGCATGTA TCAGCTAT TTATAGGGTT TTGAAAACGA TTAGCTTAAA ATCCATATAT GCTTTGTTAG TAGGTCAGGT CAGGATGGGG AAAGCCGTAG ATACTCTTTT AAGCTATCAC AGATCATCTT ATGAGCAGGT GAGGGATGCT GTTGAAGTGG TGTCCAGTTG GAAACCTTCC TGTTTCAATG TTTTCTTTCG TGTATTAGTA CTCCTATGTA GGAAAATGG

Lampiran 3. Allignment Sequense Sampel (Exon, intron dan 3UTR’)

....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 5 15 25 35 45 55 65

Vires-exon CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC Oleifera-e CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC Tenera-exo CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC Pisifera-e CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC Dura-exon CCTTACTTCT TGGGCAGTCT GGACGAGGGC TTGGACTGCT GAAGAGAACA GACATGGGGA CCTTCTCAAC Intron --- --- --- --- --- --- --- ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 75 85 95 105 115 125 135

Vir-exon-i AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT Oleifera-e AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT Tenera-exo AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT Pisifera-e AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT Dura-exon- AAGTATCTAT ACCTTTCTGG TAGAGTGGAC ATGAAGCAAA TTGAGAAAAC AATCCAATAT CTGATAGGTT Vir-intron --- --- --- --- --- --- --- ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 145 155 165 175 185 195 205

Vir-exon-i CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT Oleifera-e CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT Tenera-exo CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT Pisifera-e CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT Dura-exon- CTGGAATGGT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT Vir-intron ---GT AAGTTTATCA TGACAGTCTA GTATTTGCTC AATTTCTTTA ATTGATATTT TCCTTGTGAT ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 215 225 235 245 255 265 275

Vir-exon-i TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC Oleifera-e TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC Tenera-exo TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC Pisifera-e TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC Dura-exon- TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC Vir-intron TATAGCATCA TCAAATTCTG ATAAGAGTGA GGGTGCATTT TGCTTGCCCG AACAAGCTTC AAAGGTCGCC ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 285 295 305 315 325 335 345

Vir-exon-i AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT Oleifera-e AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT Tenera-exo AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT Pisifera-e AATCATGGAG AAACTTCTCT GAGATACGGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT Dura-exon- AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT GTTATCTTTT Vir-intron AATCATGGAG AAACTTCTCT GAGATACAGT TGTTATCTCA AAATATGGGC TTTTTTTTCT TTTATCTTTT ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 355 365 375 385 395 405 415

Vir-exon-i TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG Oleifera-e TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG Tenera-exo TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG Pisifera-e TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATATCTTGC TTGATATTAA GTTATGCCTG Dura-exon- TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG Vir-intron TTTATTTTGA ATAATTGGAG CAACTGCAAG TTACTTCAAA AATAGCTTGC TTGATATTAA GTTATGCCTG

....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 425 435 445 455 465 475 485

Vir-exon-i AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA Oleifera-e AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA Tenera-exo AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA Pisifera-e AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA Dura-exon- AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA Vir-intron AGTTCTTTTA TGTGTTCTTT TAGGTTTCTT TTTAGCCCAT TATTGGCTTA GTGTTTTATT ATCTGGATCA ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 495 505 515 525 535 545 555

Vir-exon-i AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA CTGATTGCTT CCATAATTTG Oleifera-e AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA GTGATTGCTT CCATAATTTG Tenera-exo AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA CTGATTGCTT CCATAATTTG Pisifera-e AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA GTGATTGCTT CCATAATTTG Dura-exon- AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA GTGATTGCTT CCATAATTTG Vir-intron AACATCTTAG GATAGACTAT TTTGTATCTG TTAACAAATA GTTTGAAGCA CTGATTGCTT CCATAATTTG ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 565 575 585 595 605 615 625

Vir-exon-i TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATAGGTCAC ATCAATTAAT Oleifera-e TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATAGGTCAC ATCAATTAAT Tenera-exo TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATATGTCAC ATCAATTAAT Pisifera-e TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATATGTCAC ATCAATTAAT Dura-exon- TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATATGTCAC ATCAATTAAT Vir-intron TTCAGCCGAT GCTGGAGATT GCTTGAATTC ATGGTATTTT TACTTGATTG AATAGGTCAC ATCAATTAAT ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 635 645 655 665 675 685 695

Vir-exon-i ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC Oleifera-e ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC Tenera-exo ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC Pisifera-e ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTATGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC Dura-exon- ATCACTTTCT TGTTTGCTGT CTGTAGGATC CTAGGACAGA GAACAGCCCC TACCTTGGTT TTATATACAC Vir-intron ATCACTTTCT TGTTTGCTGT CTGTAG---- --- --- --- --- ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 705 715 725 735 745 755 765

Vir-exon-i CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC Oleifera-e CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC Tenera-exo CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC Pisifera-e CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC Dura-exon- CTCATTTCAG GAGAGGGCGA CTTTCATTTC CCATGGGAAT ACTGCCAGGC ATGCCAAGGA ACATGGGGAC Vir-intron --- --- --- --- --- --- --- ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 775 785 795 805 815 825 835

Vir-exon-i GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA Oleifera-e GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA Tenera-exo GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA Pisifera-e GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA Dura-exon- GTGAAGTTGG CTCAGATATG TGGTACAATT GCCTCAGATG AGAAACGCCA TGAGACAGCT TATACTAAGA Vir-intron --- --- --- --- --- --- ---

....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 845 855 865 875 885 895 905

Vir-exon-i TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA Oleifera-e TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA Tenera-exo TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA Pisifera-e TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA Dura-exon- TCGTGGAGAA ACTGTTTGAG ATCGACCCGG ATGGTACGGT GCTTTCCTTT GCTGACATGA TGAAGAAGAA Vir-intron --- --- --- --- --- --- --- ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 915 925 935 945 955 965 975

Vir-exon-i GATCTCAATG CCTGCCCAAT TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG Oleifera-e GATCTCAATG CCTGCCCA-- TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG Tenera-exo GATCTCAATG CCTGCCCA-- TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG Pisifera-e GATCTCAATG CCTGCCCA-- TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG Dura-exon- GATCTCAATG CCTGCCCA-- TCTGATGTAT GATGGTCAGG ATGACAACCT CTTCGAGCAC TTCTCGGCAG Vir-intron --- --- --- --- --- --- --- ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 985 995 1005 1015 1025 1035 1045

Vir-exon-i TAGCCCAGCG TTTGGGTGTC TACACAGCCA AGGACTATGC CGACATACTT GAGTTCCTTA TTAATAGGTG

Dalam dokumen Marka SNAP Berbasis Fragmen Gen SAD sebagai Indikator Kandungan Asam Lemak Tidak Jenuh Kelapa Sawit (Halaman 148-179)