Simpulan
Lima isolat kapang hasil isolasi (isolat B, C, D, E dan F) menghasilkan enzim yang mampu mendegradasi serat bungkil inti sawit (BIS). Isolat F merupakan isolat yang terbaik dalam menghasilkan mannanase. Enzim isolat F yang difermentasi selama 11 hari optimal pada suhu 50 ºC, pH 6 dan relatif stabil sampai dengan suhu 40 ºC (FP-ase dan mannanase). Penambahan enzim isolat F sebanyak 2.0 mL /g bungkil inti sawit; atau setara dengan 68.8 IU Filter Paper
(FP)-ase dan 0.27 IU mannanase per 1 000 g bungkil inti sawit mampu
memperbaiki nilai nutrisi dengan meningkatnya energi metabolis bungkil inti sawit. Penambahan enzim mampu meningkatkan penampilan produksi; dan mampu mengatasi kendala serat yang tinggi pada bungkil inti sawit dengan semakin baiknya kondisi dan keadaan histologi usus halus ayam pedaging. Penggunaan 15% bungkil inti sawit dalam ransum ayam pedaging dapat dilakukan dengan penambahan enzim FP-ase dan mannanase dari isolat F.
Saran
Penggunaan bungkil inti sawit sebagai bahan pakan campuran untuk ayam pedaging sebaiknya ditambahkan enzim mannanase agar diperoleh hasil yang optimal.
broiler diets containing defatted rice bran. J Appl Poult Res 11:410–417. Ahrazem O, Prieto A, Gomez-Miranda B, Bernabé M, Antonio JL. 2001.
Comparison of cell wall polysaccharides from Nectria cinnabarina with those
from the group of Nectria with Sesquicillium anamorphs. Microbiol
147:1839–1849.
Aletor O, Ojelabi A. 2007. Comparative evaluation of the nutritive and atrributes of some traditional Nigerian Snacks and oil seed cakes. Pakistan J Nutr 6:99–103.
Alimon AR. 2005. The nutritive value of palm kernel cake for animal feed. Palm
Oil Dev 40:12–14.
Amri. 2007. Pengaruh bungkil inti sawit fermentasi dalam pakan terhadap
pertumbuhan ikan Mas (Cyprinus carpio L.). Jurnal Ilmu-ilmu Pertanian
Indonesia 9:71–76.
Amrullah IK. 2003. Nutrisi Ayam Broiler. Bogor: Lembaga Satu Gunung Budi.
Anonimous. 2004. Cobb 500 Broiler Performance and Management Guide.
Cobb-Vantress Inc. [terhubung berkala] http://www.visciukai.lt/downloads/ Eng2/broil/guide/pdf [27 Mar 2008].
Anonimous. 2006. Official Tests Confirm Cobb’s Feed Cost Benefit. [terhubung berkala]. http:
Araujo A, Ward OP. 1990a. Hemicellulases of Bacillus species: preliminary
comparative studies on production and properties of mannanases and galactanases. J Appl Bacteriol 68:253–261.
Araujo A, Ward OP. 1990b. Mannanase components from Bacillus pumilus. Appl
Environ Microbiol 56:1954–1956.
Aritonang D. 1986. Perkebunan kelapa sawit, sumber pakan ternak di Indonesia.
Jurnal Penelitian dan Pengembangan Pertanian 4:93–99.
Aro N, Saloheimo A, Ilmén M, Penttilä M. 2001. ACEII, a novel transcriptional activator involved in regulation of Cellulase and Xylanase genes of Trichoderma reesei. J Biol Chem 276:24309–24314.
Aspar HM. 2005. Malaysian palm kernel cake as animal feed. Palm Oil Dev 34:4–6.
Barnett JA, Payne RW, Yarrow D. 2000. Yeasts Characteristics and
Identification. 3rd Ed. United Kingdom: Cambridge Univ Pr.
Bauer S, Vasu P, Persson S, Mort AJ, Somerville CR. 2006. Development and application of a suite of polysaccharide degrading enzymes for analyzing plant cell walls. Proc Natl Acad Sci 103:11417–11422.
Bernabé et al. 2006. Production of Hemicellulolytic Enzymes through solid
substrate fermentation and their application in the production in balanced feed for broilers. Vet México 37:1–13.
Blair R. 2008. Nutrition and Feeding of Organic Poultry. London: UK. CAB International.
Bradner JR, Sidhu RK, Gillings M, Nevalainen KMH. 1999. Hemicellulase activity of antartic microfungi. J Appl Microbiol 87:366–370.
Brenes A, Marquardt RR, Guenter W, Viveros A. 2002. Effect of enzyme addition on the performance and gastrointestinal tract size of chicks fed lupin seed and their fractions. Poult Sci 81:670–678.
Buchanan NP, Hott JM, Kimbler LB, Moritz JS. 2007a. Nutrient composition and
digestibility of organic broiler diets and pasture forages. J Appl Poult Res
16:13–21.
Buchanan NP et al. 2007b. The effects of nonstarch polysaccharide enzyme
addition and dietary energy restriction on performance and carcass quality of organic broiler chickens. J Appl Poult Res 16:1–12.
Cappuccino JG, Sherman N. 2001. Microbiology: A Laboratory Manual. 6th Ed.
San Francisco, United State of America: Benjamin Cummings-Pearson Education, Inc.
Carvalho LPF et al. 2006. Evaluation of palm kernel meal and corn distillers
grains in corn silage-based diets for lactating dairy cows. J Dairy Sci
89:2705–2715.
Carré B. 2002. Carbohydrate Chemistry of the Feedstuffs Used for Poultry. Di
Dalam: McNab J, Boorman N, editors. Poultry Feedstuffs: Supply,
Composition and Nutritive Value. New York: CABI Publ. hlm 39–56.
Chen X, Lu W, Cao Y, Li D. 2008. Prokaryotic expression, purification and
characterization of Aspergillus sulphureus β-mannanase and site-directed
mutagenesis of the catalytic residues. Appl Biochem Biotechnol 149:139–144. Choct, M. 2001. Enzyme Supplementation of Poultry Diets Based on Viscous
Cereals. Di Dalam: Enzymes in Farm Animal Nutrition. Bedford MR, Patridge
GG. Editors. New York: CABI Publ. hlm 145–160.
Choct M, Hughes RJ, Trimble RP, Angkanaporn K, Annison G. 1995. Non starch polysaccharide degrading enzymes increase the performance of broiler chickens fed wheat of low apparent metabolizable energy. J Nutr 125:485–492. Choct M et al. 1996. Increased small intestinal fermentation is partly responsible
for the anti nutritive activity of non starch polysaccharides in chickens. Br Poult Sci 37:609–621.
Choct M, Hughes RJ, Bedford MR. 1999. Effects of a xylanase on individual bird variation, starch digestion, throughout intestine and ileal and caecal volatile fatty acid production in chickens fed wheat. Br Poult Sci 40:419–422.
Choct M, Kocher A. 2000. Non starch carbohydrates: digestion and its secondary effects in monogastrics. Proceedings of the Nutrition Society of Australia. 24:31–38. [terhubung berkala]. http//www. Healthy-eatingclub.org/APJCN/ Proc.Nut.Soc/2000/Choct/31/pdf [27 Feb 2008].
Chong CH. 1999. Improving utilization of poultry feedstuffs with supplemental amino acid and enzymes [tesis]. Winnipeg Manitoba, Canada: The Faculty of Graduate Studies Department of Animal Science, Univ of Manitoba.
Clausen CA, Green III F, Highley TL. 1991. Extracellular polysaccharide degrading enzymes of Postia placenta isolated from wood or artificial media.
Di dalam: Llewellyn GC, Dashek WV, O’Rear CE, Editor. Biodeterioration
Research 4: Mycotoxins, Wood Decay, Plant Stress, Biocorrosion and General Biodeterioration. Proceedings of 4th meeting of the Pan American Biodeterioration Society [simposium elektronik]. New York, 20–25 Agus 1991. Plenum Pr. hlm 231–242.
Cooper MA, Washburn KW. 1998. The relationships of body temperature to weight gain, feed consumption and feed utilization in broilers under heat stress. Poult Sci 77:237–242.
Corzo A et al. 2005a. Dietary amino acid density effects on growth and carcass of broilers differing in strain cross and sex. J Appl Poult Res 14:1–9.
Corzo A, Kidd MT, Koter MD, Burgess SC. 2005b. Assessment of dietary amino acid scarcity on growth and blood plasma proteome status of broiler chickens. Poult Sci 84:419–425.
Dänicke S, Simon O, Jeroch H, Bedford MR. 1997. Interactions between dietary fat type and xylanase supplementation when rye based diets are fed to broiler chickens. 2. performance, nutrient digestibility and the fat soluble vitamin status of livers. Br Poult Sci 38:546–556.
Daskiran M, Teeter RG, Fodge D, Hsiao HY. 2004. An evaluation of endo-β
-D-mannanase (Hemicell) effects on broiler performance and energy use in diets varying in β-mannan content. Poult Sci 83:662–668.
Daud MJ, Jarvis MC. 1992. Mannan of oil palm kernel. Phytochem 31:463–464.
Davison F, Magor KE, Kaspers B. 2008. Structure and Evolution of Avian
Immunoglobulins. Di Dalam: Davison F, Kaspers B, Schat KA, Editors. Avian
Immunology. London: Academic Pr. hlm 107–127.
De Nicolás-Santiago S, et al. 2006. Physiological, morphological and mannanase
production studies on A. niger uam-gs1 mutants [hasil penelitian]. Electronic J
Biotechnol 9:50–60 [terhubung berkala].
content/vol9/issue1/full/2/pdf [1 Nov 2007].
[Deptan] Departemen Pertanian. 2005. Prospek dan Arah Pengembangan
Agribisnis Unggas. Jakarta: Balitbangtan, Departemen Pertanian.
[Deptan] Departemen Pertanian. 2010. Pusat Data dan Informasi Pertanian: Komoditi Perkebunan; Kelapa Sawit. [terhubung berkala] http://database. go.id/bdsp/newkom.asp. [4 Feb 2010].
Dewi GAMK. 1998. Studi pemanfaatan asam lemak sawit sebagai pakan ternak ayam [disertasi]. Bogor: Sekolah Pascasarjana Institut Pertanian Bogor.
Dibner JJ, Richards JD. 2004. The digestive system: Challenges and opportunities. J Appl Poult Res 13:86–93.
Düsterhöft EM, Posthumus MA, Voragen AGJ. 1992. Non-starch polysaccharides
from Sunflower (Helianthus annuus) meal and palm kernel (Elaeis
guineensis) meal - Investigation of the structure of major polysaccharide. J Sci Food Agric 59:151–160.
Esuga PM, Sekoni AA, Omage JJ, Bawa GS. 2008. Evaluation of enzyme
Maxigrain® Supplementation of graded levels of palm kernel meal (PKM) on
the performance of broiler chickens. Pakistan J Nutr 7:607–613.
Ezieshi EV, Olomu JM. 2004. Comparative performance of broiler chickens fed varying levels of palm kernel cake and maize offal.Pakistan J Nutr 3:254–257. Ezieshi EV, Olomu JM. 2008. Nutritional evaluation of palm kernel meal types: 2.
effects on live performance and nutrient retention in broiler chicken diets. African J Biotechnol 7:1171–1175.
Farrel DJ. 1978. Rapid determination of metabolizale energy of foods using cockerels. Br Poult Sci 19:303–308.
Forbes JM. 2007. Voluntary Food Intake and Diet Selection in Farm Animals. 2nd
Ed. Biddles Ltd., Guildford, United Kingdom: CAB International.
Gauthier R. 2002. Intestinal health, the key to productivity: the case of organic
acids. Precongreso Cientifico Avicola IASA 27th Convencion ANECA-WPDC;
Puerto Vallarta, Mexico, 30 Apr 2002 [terhubung berkala]. http//www.jefo. capdfIntestinal_ Health.pdf [26 Nov 2007].
Ghose TK. 1987. Measurement of cellulase activities. Pure Appl Chem 59:257–268. Glick B. 2000. Immunophysiology. Di Dalam: Sturkie’s Avian Physiology.5th Ed.
Sturkie PD, Editor. London: Academic Pr. hlm 657–670.
Grist A. 2006. Poultry Inspection-Anatomy, physiology and disease conditions.
2nd Ed. Nottingham, United Kingdom: Nottingham Univ Pr.
Gűnal M, Yaşar S. 2004. Performance and some digesta parameters of broiler chickens given low or high viscosity wheat-based diets with or without
enzyme supplementation. Turk J Vet Anim Sci 28:323–327.
Harjunpää V. 1998. Enzymes Hydrolysing Woods Polysaccharides. A progress
curve study of oligosaccharide hydrolysis by two cellobiohydrolases and three
β-mannanases [disertasi]. Helsinki, Finland: Valtion teknillinen
tutkimuskeskus (VTT) Chemical Technology Department of Chemistry Faculty of Science, Univ of Helsinki.
Hazelwood RL. 2000. Pancreas. Di Dalam: Sturkie’s Avian Physiology.5th Ed. Sturkie PD, Editor. London: Academic Pr. hlm 539–555
He X et al. 2008. Inducible and constitutive expression of a novel thermostable
alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5 in Pichia pastoris
and characterization of the recombinant enzyme. Enzyme Microb Technol
Hidalgo MA, Dozier III. WA, Davis AJ, Gordon RW. 2004. Live performance and meat yield responses of broilers to progressive concentrations of dietary energy maintained at a constant metabolizable energy to crude protein ratio. J Appl Poult Res 13:319–327.
Hughes RJ. 2003. Energy Metabolism of Chickens: Physiological Limitations.
[publikasi RIRDC No. 02/151]. Australia: Rural Industries Research and Development Corporation [terhubung berkala]. http//www/rirdc/gov/au [27 Mar 2008].
Hughes RJ, Choct M. 1999. Chemical and physical characteristics of grains that are related to variability in energy and amino acid availability in poultry. Aust J Agric Res 50:689–701.
Iji PA. 1999. The impact of cereal non-starch polysaccharides on intestinal development and function in broiler chickens. World's Poult Sci 55:375–387. Iji PA, Hughes RJ, Choct M, Tivey DR. 2001. Intestinal structure and function of
broiler chickens on wheat-based diets supplemented with a microbial enzyme. Asian-AustJ Anim Sci 14:54–60.
Immanuel G, Bhagavath CMA, Raj PI, Esakkiraj P, Palavesam A. 2007. Production and partial purification of cellulase by Aspergillus niger and A. fumigatus fermented in coir waste and sawdust. Internet J Microbiol. Vol.3. http://www. ispub.com/ostia/ index.php.journals/ijmb/vol3n1/cellulase. xml. [28 Okt 2008].
Irawadi TT. 1990. Pemanfaatan limbah kelapa sawit sebagai media pertumbuhan kapang penghasil enzim ekstraselular [laporan penelitian]. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.
Iyayi EA. 2004. Changes in the cellulose, sugar and crude protein contents of
agro-industrial by-products fermented with Aspergillus niger, Aspergillus
flavus and Penicillium sp. African J Biotechnol 3:186–188.
Iyayi EA, Aderolu ZA. 2004. Enhancement of the feeding value of some agroindustrial by products for laying hens after their solid state fermentation with Trichoderma viride. African J Biotechnol 3:182–185.
Iyayi EA, Davies BI. 2005. Effect of enzyme supplementation of palm kernel meal and brewer’s dried grain on the performance of broilers. Int J Poult Sci 4:76–80.
Jackson ME, Geronian K, Knox A, McNab J, McCartney E. 2004. A dose
response study with the feed enzyme β-mannanase in broilers provided with
corn-soybean meal based diets in the absence of antibiotic growth promoters. Poult Sci 83:1992–1996.
Jaelani A. 2007. Peningkatan kualitas bungkil inti sawit oleh kapang Trichoderma reesei sebagai pendegradasi polisakarida mannan dan pengaruhnya terhadap penampilan ayam pedaging [disertasi]. Bogor: Sekolah Pascasarjana Institut Pertanian Bogor.
Judoamidjojo M, Darwis AA, Sa’id EG. 1992. Teknologi Fermentasi. Jakarta:
Keller FA, Hamilton JE, Nguyen QA. 2003. Microbial pretreatment of biomass.
potential for reducing severity of thermochemical biomass pretreatment. Appl
Biochem Biotechnol 105:27–41.
Khanongnuch C, Sa-nguansook C, Lumyong S. 2006. Nutritive quality of β
-mannanase treated copra meal in broiler diets and effectiveness on some fecal bacteria. Int J Poult Sci 5:1087–1091.
King RD, Moughan PJ. 1998. The effect of feed enzyme supplementation on the performance of meat chickens fed enzyme-responsive barley and wheat/maize based diets. NZ J Agric Res 41:395–403.
Kismono, MMSS. 1986. Toleransi ayam broiler terhadap kandungan serat kasar, serat detergent asam, lignin dan silika dalam ransum yang mengandung tepung daun alang-alang [disertasi]. Bogor: Fakultas Pascasarjana Institut Pertanian Bogor.
Laganá C, Ribeiro AML, Kessler AM, Kratz LR, Pinheiro CC. 2007. Effects of the reduction of dietary heat increment on the performance, carcass yield and diet digestibility of broilers submitted to heat stress. Brazil J Poult Sci 1:45–51. La Grange DC, Pretorius IS, Claeyssens M, Van Zyl WH. 2001. Degradation of
Xylan to D-Xylose by Recombinant Saccharomyces cerevisiae coexpressing
the Aspergillus niger β-Xylosidase (xlnD) and the Trichoderma reesei Xylanase II (xyn2) genes. Appl Environ Microbiol 67:5512–5519.
Lee JT, Bailey CA, Cartwright AL. 2003. β-mannanase ameliorates
viscosity-associated depression of growth in broiler chickens fed guar germ and hull fractions. Poult Sci 82:1925–1931.
Lee JT, Connor-Appleton S, Bailey CA, Cartwright AL. 2005. Effects of guar
meal by-product with and without β-mannanase hemicell on broiler
performance. Poult Sci 84:1261–1267.
Leeson S, Summers JD. 2005. Commercial Poultry. 3rd Ed. Canada, Guelph,
Ontario. Univ Books.
Mathlouthi N, Lallẻs JP, Lepercq P, Juste C, Larbier M. 2002a. Xylanase and β -glucanase supplementation improve conjugated bile acid fraction in intestinal contents and increase villus size of small intestine wall in broiler chickens fed a rye based diet. J Anim Sci 80:2773–2779.
Mathlouthi N, Mallet S, Saulnier L, Quemener B, Larbier M. 2002b. Effects of xylanase and β -glucanase addition on performance, nutrient digestibility and physico-chemical conditions in the small intestine contents and caecal
microflora of broiler chickens fed a wheat and barley-based diet. Anim Res
51:395–406.
Maria SJC et al. 2006. Galactanases and mannanases improve the nutritive value
of maize and soybean meal based diets for broiler chicks. J Poult Sci 43:344–350. Marquardt RR, Brufau J. 2007. Future of feed enzymes: orientation and
perspectives. [review article] CIHEAM-Options Mediterraneennes. hlm 181–
187. [terhubung berkala]. http//ressources.ciheam.org/om.pdf.c2697605984. pdf [24 Nov 2007].
Marquardt RR, Bedford MR. 2001. Future Horizons. Di dalam: Enzymes in Farm
Animal Nutrition. Bedford MR dan Partridge GG, Editors. Wallingford,
United Kingdom: CAB International. hlm 389–398.
McKinney LJ, Teeter RG. 2004. Predicting effective caloric value of nonnutritive factors: I. pellet quality and II. prediction of consequential formulation dead zones. Poult Sci 83:1165–1174.
Meng X, Slominski BA, Guenter W. 2004. The effect of fat type, carbohydrase and lipase addition on growth performance and nutrient utilization of young broilers fed wheat-based diets. Poult Sci 83:1718–1727.
Meng X, Slominski BA, Nyachoti CM, Campbell LD, Guenter W. 2005. Degradation of cell wall polysaccharides by combinations of carbohydrase enzymes and their effect on nutrient utilization and broiler chicken performance. Poult Sci 84:37–47.
Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of
reducing sugar. Anal Chem 31:426–428.
Mirwandhono E, Siregar Z. 2004. Pemanfaatan hidrolisat tepung kepala udang
dan limbah kelapa sawit yang difermentasi dengan Aspergillus niger,
Rizhopus oligosporus dan Trichoderma viridae dalam ransum ayam pedaging [laporan penelitian]. Medan: Univ Sumatera Utara Digital Library.
Moran ET. 1985. Digestive physiology of Duck. Di dalam: Duck Production and
World Practice. Farrel DJ, Stapleton P, Editors. Armidale: Univ of England.
Moussa TAA, Tharwat NA. 2007. Optimization of cellulase and β-glucosidase
induction by sugarbeet pathogen Sclerotium rolfsii. African J Biotechnol
6:1048–1054.
Mudau MM. 2006. The production, purification and characterization of endo-1,4-β-mannanase from newly isolated strains of Scopulariopsis candida [tesis]. South Africa: Faculty of Natural and Agricultural Sciences Department of Microbial, Biochemical and Food Biotechnology Univ of the Free State Bloemfontein.
Mudau MM, Setati ME. 2008. Partial purification and characterization of endo-β
-4-mannanases from Scopulariopsis candida strains isolated from solar
salterns. African J of Biotechnol 7:2279–2285.
Nahas J, Lefrançois MR. 2001. Effects of feeding locally grown whole barley with or without enzyme addition and whole wheat on broiler performance and carcass traits. Poult Sci 80:195–202.
Narasimha G, Sridevi A, Viswanath B, Chandra MS, Reddy BS. 2006. Nutrient
effects on production of cellulolytic enzymes by Aspergillus niger. Afr J
Biotec 5:472–476.
[NRC] National Research Council. 1994. Nutrient Requirements of Poultry. 9th Ed rev. Washington DC: National Academy Pr.
Ng WK, Chong KK. 2002. The nutritive value of palm kernel meal and the effect of enzyme supplementation in practical diets for Red Hybrid Tilapia (Oreochromis sp.). Asian Fisheries Sci 15:167–176.
Nipa MN, Sultana S, Islam SMR, Hakim MA. 2006. Cellobiose Octaacetate
(COA) enhance the production of cellulase by Aspergillus fumigatus. Eur J
Sci Res 15:287–294.
Nogawa M, Yatsui K, Tomioka A, Okada H, Morikawa Y. 1999. An α
-L-Arabinofuranosidase from Trichoderma reesei containing a noncatalytic
Xylan-binding domain. Appl Environ Microbiol 65:3964–3968.
Nwokolo EN, Bragg DB, Saben HS. 1976. The availability of amino acid from palm kernel meal, soybean meal, cotton seed meal and rapseed meal for growing chick. Poult Sci 55:2300–2304.
Odetallah NH, Ferket PR, Grimes JL, McNaughton JL. 2002. Effect of
mannan-endo-1,4-β-mannosidase on the growth performance of turkeys fed diets
containing 44 and 48% crude protein soybean meal. Poult Sci 81:1322–1331.
Ohtani S, Leeson S. 2000. The effect of intermittent lighting on metabolizable energy intake and heat production of male broilers. Poult Sci 79:167–171. Okeudo NJ, Eboh KV, Ndidi VI, Akanno EC. 2005. Growth rate, carcass
characteristics and organoleptic quality of broiler fed graded levels of palm kernel cake. Int J Poult Sci 4:330–333.
Okeudo NJ, Onyike IL, Okoli CV, Chielo IL. 2006. Production performance, meat quality and feed cost implications of utilizing high levels of palm kernel cake in broiler finisher diets. Int J Poult Sci 5:1160–1163.
Oláh I, Vervelde L. 2008. Structure of the Avian Lymphoid System. Di Dalam:
Davison F, Kaspers B, Schat KA, Editors. Avian Immunology. London:
Academic Pr. hlm 13–50.
Omojola AB, Adesehinwa AOK. 2007. Performance and carcass characteristics of
broiler chickens fed diets supplemented with graded levels of Roxazyme G®.
Int J Poult Sci 6:335–339.
Onifade AA, Babatunde GM. 1998. Comparison of the utilisation of palm kernel
meal, brewers’ dried grains and maize offal by broiler chicks. Br Poult Sci
39:245–250.
Onwudike OC. 1986. Palm kernel meal as a feed for poultry. 3. replacement of
groundnut cake by palm kernel meal in broiler diets [abstrak].Anim Feed Sci
Technol 16:195–202. [terhubung berkala].
science/ journal [30 Sep 2007].
Onwudike OC. 1988. Palm kernel meal as a feed for poultry. 4. Use of palm
kernel meal by laying birds [abstrak]. Anim Feed Sci Technol 20:279–286.
[terhubung berkala]
Orunmuyi M, Bawa GS, Adeyinka FD, Daudu OM, Adeyinka IA. 2006. Effects of graded levels of palm kernel cake on performance of grower rabbits. Pakistan J Nutr 5:71–74.
Palonen H. 2004. Role of lignin in the enzymatic hydrolysis of lignocellulose [disertasi]. Helsinki, Finlandia: Valtion teknillinen tutkimuskeskus (VTT) Biotechnology, Univ of Technology.
Panigrahi S. 1991. Behaviour changes in broiler chicks fed on diets containing
palm kernel meal [abstrak]. Appl Anim Behav Sci 31:277–281. [terhubung
berkala]
Panigrahi S, Powell CJ. 1991. Effects of high rates of inclusion of palm kernel meal in broiler chick diets. Anim Feed Sci Technol 34:37–47.
Parsons CML, Potter LM, Bliss BA. 1984. A modified voluntary feed intake bioassay for determination of metabolizable energy with leghorn roosters. Poult Sci 63:1610–1616.
Pelczar MJJr, Chan ECS. 1986. Dasar-dasar Mikrobiologi. Volume ke-1.
Hadioetomo RS, Imas T, Tjitrosomo SS, Angka SL, penerjemah; Jakarta:
Univ Indonesia Pr. Cetakan 1. Terjemahan dari: Elements of Microbiology.
Perez JF, Gernat AG, Murillo JG. 2000. The effect of different levels of palm kernel meal in layer diets. Poult Sci 79:77–79.
Pettersson D, Graham H, Aman P. 1990. Enzyme supplementation of low or high crude protein concentration diets for broiler chickens. Anim Prod 51:399–404. Pirgozliev VR, Rose SP, Graybosch RA. 2002. Energy and amino acid
availability to chickens of waxy wheat. Arch Geflügelk 66:108–113.
Pope T, Emmert JL. 2002. Impact of phase-feeding on the growth performance of broilers subjected to high environmental temperatures. Poult Sci 81:504–511.
Prendecka M, Buczyńska A, Rogalski J. 2007. Purification and characterization of
β-mannosidases from white rot fungus Phlebia radiate. Polish J Microbiol
56:139–147.
Puchart et al. 2004. Purification and characterization of two forms of endo-β
-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708.
Biochim Biophys Act 1674:239–250.
Ramli N, Takegawa K, Iwahara S. 1994. Degradation of unsaturated
polysaccharide derived from acidic polysaccharide of Fusarium sp. M7-1 by a
bacterium isolated from soil. J Ferment Bioeng 5:572–574.
Rättö M, Poutanen K. 1988. Production of mannan-degrading enzymes. Biotechnol Letters 10:661–664.
Regalado C et al. 2000. Production, partial purification and properties of β -mannanases obtained by solid substrate fermentation of spent soluble coffee wastes and copra paste using Aspergillus oryzae and Aspergillus niger. J Sci Food Agric 80:1343–1350.
Ritschkoff AC. 1996. Decay Mechanisms of Brown-Rot Fungi [Technical