PEMBAHASAN UMUM
SIMPULAN DAN SARAN
Simpulan
1. Penyediaan bibit calon varietas unggul hasil selfing atau persilangan dapat dilakukan melalui induksi plbs dari eksplan daun planlet dari individu-individu
dalam populasi tersebut.
2. Populasi F2 dari selfing F1 hibrid (Ph. Phuket Beauty x Ph. Zauberrose)
3. Marka molekuler dapat digunakan untuk melacak tetua dari suatu aksesi hibrida.
menunjukkan keragaman genetik yang rendah yang mengindikasikan tetua persilangan memiliki kekerabatan genetik yang dekat.
Saran
Untuk mendapat informasi yang lebih akurat mengenai keragaman genetik anggrek Phalaenopsis, perlu penambahan jumlah primer dan identifikasi primer-
primer yang polimorfik untuk tetua 1 (P1) dan tetua 2 (P2) yang akan digunakan dalam identifikasi F1 dan progeni F2.
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Lampiran 1. Primer dan sekuen yang digunakan untuk mendeteksi polimorfisme pada Anggrek Phalaenopsis
No Primer Produk PCR (bp) Sekuen primer Tm (0C) 1 IPS1 129 F=GAGAGAGAAAGAGAGAGGTACG R=CTACGGCTGATTTGATTTCTA 54 2 IPS2 136 F=AGAGAGAGAAAGAGAGAGATGT R=CTACGGCTGATTTGATTTCTA 50 3 PGA06 175 F=GTGAAAGACACACACACACACA R=GGTTGTACGCCTTTGTCGAT 58 4 PGVB11 130 F=TGCATTCTTGCTTCGTTTTG R=CGAGAAGCATTAAGCTGCATT 59 5 PGCT13 175 F=GGAAGGGCTCTCTCTCTCTCT R=TTGCCCTCCTCTCTTTCTTT 58 6 IPS4 127 F=GAGAGAGAAAGAGAGAGAAACAT R=CTACGGCTGATTTGATTTCTA 54 7 IPS5 127 F=GAGAGAGAAAGAGAGAGAAACAT R=CTACGCCTGATTTGATTTCTA 54 8 IPS6 139 F=GCTAGAGATAGAGAGAGAAAGAG R=CTACGGCTGATTTGATTTCTA 54 9 IPS7 140 F=GTTTGTTGTCAGAGAAGCATT R=AATTGAGACCTTCACTTCCTC 54 10 IPS9 158 F=GAGAGAGAAAGAGAGAGAAACAT R=CTACGGCTGATTTGATTTCTA 54 11 IPS10 130 F=AGAGAGAGAAAGAGAGAGATGC R=CTACGCCTGATTTGATTTCTA 54 12 IPS13 137 F=GCTAGAGATAGAGAGAGAAAGAG R=CTACGGCTGATTTGATTTCTA 55 13 IPS14 160 F=TAGTTTGTTTCGTGGTTCAGT R=TGGTGTGAATTATCTCGTTCT 55 14 IPS15 147 F=CGTTTCACTCAAAGTTTTGTT R=TCCTGACGTAATATTTGTTGG 55 15 IPS51 160 F=TTGGCAGCGTTAAAGATATAG R=CATCCTCTGCATAAAAATCAC 55 16 IPS52 180 F=GCAATGGAGAAAAAGGATTTA R=GCTCCACTCACCTGTTAGTTA 55 17 PeGBMS 281 248 F=GAGGAAGGAGAAGGGAATGG R=GCGAAGAAGAAATCCGTGAA 61 18 PeGBMS 282 154 F=GTAGGGGTCTTGCGTGTGTT R=CATTTAGATACAACACCGCACG 61 19 PeGBMS 296 222 F=ACCTTCTTCCCGTCTATGTGTG R=TGTTCAGTCGGGCATTCG 64 20 PeGBMS 310 208 F=AGCCTCCGACCATTCTTTTT R=GGATGTATTTTGGGGCAGAA 61
No Primer Produk PCR (bp) Sekuen primer Tm (0C) 21 PeGBMS 315 334 F=TCAGGTGGTGGAACGAGAA R=TCATTTAGACCGTGGGAAGC 63 22 PeGBMS 322 216 F=GGCAAAAAGAAAGAACCCACT R=TTCCTCGGGTTCAGATTCAG 62 23 PeGBMS 343 443 F=CGGGGGTCCATCAAAAGA R=TGGGGAAGGAGTGGGCT 64 24 PeGBMS 352 322 F=CAGGGACTTGAGCCAGAGAA R=CACTCTCACATTTACGGGACAA 64 25 PeGBMS 361 224 F=GGGCATTTCAACTCGCTTAG R=GTTGTAATGCGGGGAGAGG 62 26 PeGBMS 380 223 F=GCTTCGGGTTTTGTAGGCA R=CGAAAACCATCCTCCCTACA 62 27 PeGBMS 402 317 F=TGGGAAAAGGACAACGAAA R=TGGCGGACAAGATTAGAGAGA 61 28 PeGBMS 445 329 F=TTTTCACGCATTCCTACCG R=TTTTTGGAGTTGGGCTTGAG 61 29 PeGBMS 466 124 F=CGTCAGCCATCCTCCTTTT R=TGTTAGTCATTGCCCATCTTCA 62 30 PeGBMS 478 201 F=TCACTTGTTCTCGGTTCTTTCA R=GGGAGGGAGGGTGGTAGTTA 61 31 PeGBMS 483 341 F=CCCAACCTGCTTCCCTATTT R=AAAAGAAATCCCCAAAGGCT 61 32 PeGBMS 484 230 F=CATAGGATTGAAGGGTTGGAA R=TCTCAGTCACCATCCTCTTCC 60 33 PeGBMS 502 275 F=ACGAGCAGGTGAGATGTGTT R=TAGCAATCACCACCACCTCTC 64 34 PeGBMS 552 196 F=CCCTTCCACCAAATCTTCCT R=TCACGGGTTCAAATCCTCTC 63 35 PeGBMS 558 333 F=CAGGAAGAGGAACCAGCAAG R=TTTGGGGATTTTATGTGGAGT 60 36 PeGBMS 574 219 F=TTTTTGACACTGCCTGCCA R=AGAGTTGATGTGGCGGGG 64 Sumber : Fatimah dan D. Sukma 2011 ; Hsu et al. 2011
Lampiran 2. Komposisi media dasar Murashige dan Skoog (MS)
Stok Bahan Kimia Konsentrasi (g L-1) Pemakaian
A NH4NO3 82.500 1650.000 B KNO3 95.000 1950.000 C KH2PO4 H3BO3 KI Na2MoO4 CaCl2.6H2O 34.000 1.240 0.166 0.050 0.005 170.000 6.200 0.830 0.250 0.025 D CaCl2.2H2O 88.000 440.000 E MgSO4.7H2O MnSO4.H2O ZnSO4.7H2O CuSO4.5H2O 74.000 3.380 1.720 0.005 370.000 16.900 8.600 0.025 F Na EDTA.2H2O FeSO4.7H2O 3.720 2.780 37.200 27.800 Vitamin Myo-inositol Thiamin HCl Piridoksin HCl Asam Nicotin Glisin 10.000 0.010 0.050 0.050 0.200 100.000 0.100 0.500 0.500 2.000 Sumber : Murashige dan Skoog 1962
Lampiran 3. Komposisi air kelapa
No Komposisi Kimia Konsentrasi (mg/100 g)
1 Gula total 2.61* 2 Kalsium 24 3 Besi 0.29 4 Magnesium 25 5 Fosfat 20 6 Kalium 240 7 Natrium 105 8 Seng 0.1 9 Tembaga 0.04 10 Mangan 0.142 11 Selen 0.001 12 Asam askorbat 2.4 13 Tiamin 0.03 14 Riboflavin 0.057 15 Niasin 0.08 16 Asam pantotenat 0.043 17 Piridoksina 0.032 18 Folat total 0.03 19 Lipit total 0.2* 20 Alanina 0.037* 21 Arginina 0.118* 22 Asam aspartat 0.07* 23 Sistina 0.014* 24 Asam glutamina 0.165* 25 Glisina 0.034* 26 Histidina 0.017* 27 Isoleusina 0.028* 28 Leusina 0.053* 29 Lisina 0.032* 30 Metionina 0.013* 31 Fenilanina 0.037* 32 Prolina 0.03* 33 Serina 0.037* 34 Tirosina 0.022* 35 Triptofan 0.008* 36 Protein 0.72* 37 Treonina 0.026* 38 Valina 0.044* 39 1.3-Diphenylurea Ada