SIMPULAN UMUM
R. leguminosarum ANU845 yang telah direkayasa mampu mentransfer gen dengan efektifitas yang sama dengan A. tumefaciens LBA288, sehingga memungkinkan untuk digunakan sebagai agen transformasi alternatif untuk rekayasa genetika tanaman. Seperti halnya terformasi Agrobacterium, maka tingkat keberhasilan transformasi menggunakan Rhizobium dipengaruhi oleh genotipe tanaman yang digunakan
Analisis bioinformatik promoter OsHox6 mengindikasikan bahwa aktivitas promoter OsHox6 diregulasi oleh kekeringan dan ABA. Pola ekspresi gen GUSPlus, menunjukkan aktivitas promoter OsHOx6 meningkat pada saat ada cekaman kekeringan dan aktif pada jaringan meristem.
Meskipun skenario utuh mekanisme molekuler toleran kekeringan yang disebabkan oleh OsHox6 masih belum sepenuhnya diketahui, tingkat survival rate pada fase vegetatif mengindikasikan bahwa OsHox6 bertanggung jawab dalam menentukan sifat toleran kekeringan pada tanaman padi.
SARAN
Agar transformasi genetik menggunakan Rhizobium dapat lebih luas digunakan pada tanaman maka perlu menggunakan plasmid biner yang lebih kecil dan lebih mudah dimanipulasi secara in vitro.
Evaluasi lebih lanjut ketahanan tanaman transgenik stabil (homozigot) yang mengoverekspresikan OsHox6 pada fase generatif terhadap kekeringan perlu dilakukan. Selanjutnya analisis fenotip tanaman padi yang tidak mengekspresikan gen OsHox6 perlu dilakukan untuk melengkapi data fungsi gen OsHox6 sehingga penjelasan tentang peranan gen OsHox6 pada tanaman padi lebih komprehensif. Identifikasi gen-gen yang ekspresinya berubah pada tanaman yang mengoverekspresikan OsHox6 membantu untuk menjelaskan mekanisme gen OsHox6 dalam mengendalikan sifat toleran kekeringan.
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