PEMBUATAN MENTEGA COKELAT TIRUAN
DENGAN PROSES INTERESTERIFIKASI ENZIMATIK
Teknologi
-
The more abundance palm oil supply in Indonesia in one hand and the higher demand of cocoa on the other hand, has resulted in the requirement to diversify palm oil's products. Cocoa butter
is one of the choices. Therefore, the main aim of this study is to create a reliable technological process.
The study is an explorative research. The basic principle in the preparation of CBE is an effort to produce the major triglyceride components of CBE namely 1,3 (POP),
(POS), and in particular
composition and concentrations.
There were several steps in the exploration of CBE preparation. The one was the approach through the concentration increment of triglyceride POP from by mean of the enzymatically acidolysis reaction with acid , the second one was the concentration increment of the monoglyceride 2-mono-oleat by mean of the enzymatic hydrolysis of using a specific lipase-1,3 , the third one was acidolysis of POP rich acidolysis of 2-mono-oleat as well as the direct acidolysis of with stearic acid) which was followed by the experiments using our own designed packed bed reactor for one of the best results of reaction mixture.
Having trimmed the less successful results, the main results of this study can be summarized as follows. The reaction acidolysis of stearic incorporation into a glyceride mixture) required a
condition which was achieved by utilizing either anhydrous sodium sulphate and/or molecular sieves. Small scale experiments of process in shaken flasks revealed two best conditions. Firstly, the use of stearic ratio of 0.5: 1.0 in 30 ml hexane for 60 hr, with 10 % of (on
basis which was 6 g), temperature of 55 at rotation of 250 Secondly, the use of stearic
ratio of in 50 ml hexane for 48 hr, with the rest conditions were the same as in the first case. The CBE indexes achieved were 55.8 and 57.5 for the first and the second respectively, while the composition of
were and for the and the second
respectively. A scale up study for these two best conditions and 20 X the first, and 20 X for the second) showed the decrease of the CBE index to become around 30 and 17 for the first and the second respectively. Among the scale-up results, the compositions were relatively close one among the others, but with lower concentrations as compared to the original On the development process using our own bioreactor (packed bed reactor with recirculation), the best result needed 6 hr reaction time and resulted in CBE index of 5 1.1 1 having of 1 It happened here (and in the scale up process too) that the concentrations of POP, POS and SOS decreased dramatically as compared to those of original shaken flasks. It was probably due to the lesser intensity of molecular
PEMBUATAN MENTEGA COKELAT TIRUAN
DENGAN PROSES INTERESTERIFIKASI ENZIMATIK
Teknologi
-
The more abundance palm oil supply in Indonesia in one hand and the higher demand of cocoa on the other hand, has resulted in the requirement to diversify palm oil's products. Cocoa butter
is one of the choices. Therefore, the main aim of this study is to create a reliable technological process.
The study is an explorative research. The basic principle in the preparation of CBE is an effort to produce the major triglyceride components of CBE namely 1,3 (POP),
(POS), and in particular
composition and concentrations.
There were several steps in the exploration of CBE preparation. The one was the approach through the concentration increment of triglyceride POP from by mean of the enzymatically acidolysis reaction with acid , the second one was the concentration increment of the monoglyceride 2-mono-oleat by mean of the enzymatic hydrolysis of using a specific lipase-1,3 , the third one was acidolysis of POP rich acidolysis of 2-mono-oleat as well as the direct acidolysis of with stearic acid) which was followed by the experiments using our own designed packed bed reactor for one of the best results of reaction mixture.
Having trimmed the less successful results, the main results of this study can be summarized as follows. The reaction acidolysis of stearic incorporation into a glyceride mixture) required a
condition which was achieved by utilizing either anhydrous sodium sulphate and/or molecular sieves. Small scale experiments of process in shaken flasks revealed two best conditions. Firstly, the use of stearic ratio of 0.5: 1.0 in 30 ml hexane for 60 hr, with 10 % of (on
basis which was 6 g), temperature of 55 at rotation of 250 Secondly, the use of stearic
ratio of in 50 ml hexane for 48 hr, with the rest conditions were the same as in the first case. The CBE indexes achieved were 55.8 and 57.5 for the first and the second respectively, while the composition of
were and for the and the second
respectively. A scale up study for these two best conditions and 20 X the first, and 20 X for the second) showed the decrease of the CBE index to become around 30 and 17 for the first and the second respectively. Among the scale-up results, the compositions were relatively close one among the others, but with lower concentrations as compared to the original On the development process using our own bioreactor (packed bed reactor with recirculation), the best result needed 6 hr reaction time and resulted in CBE index of 5 1.1 1 having of 1 It happened here (and in the scale up process too) that the concentrations of POP, POS and SOS decreased dramatically as compared to those of original shaken flasks. It was probably due to the lesser intensity of molecular
