the
of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Agricultural University, Campus, 16680
-
Indonesia:
(SOD) is one of the
radical scavenger which catalyzes anion radical generated
SOD thus eliminates from
reported that SOD was immunohistol localized in the human and rat
tissues 1989; Wresdiyati
1997). SOD was also reported plays role in physiological
Some cases of failed pregnancy in human was by the decreasing level of SOD (Sugino et 2000). of SOD was also
reported in condition such
as stress, diabetes and
(Wresdiyati et 2002; Wmsdiyati 2003; Wresdiyati 2003;
Wresdiyati et in neoplastic tissues
and neuron of in pancreatic tissues of Macaca fascicularis.
Materials and Methods
The liver, kidney, testis, adrenal gland,
heart,
lung, and pancreatic tissues of Macacafascicularis were obtained and fixed in
solution
for
24 After fixation,the
tissues were then dehydrated in aseries
of alcoholsand cleared in The tissues were then
embedded in paraffin before microtome sectioning (4 thickness). Tissue
were immunohistochemically stained for
as described previously
et 1989; Wresdiyati et 2008) with
modifications. The tissue sections were washed for 15 min with 3 changes of
each step. After and
rehydration, the tissue sections were exposed
to 3% for min to inactivate endogenous peroxidase activity and then to normal goat serum to block nonspecific binding. rinsing with PBS, the tissue
sections were incubated with the primary
antibody of (Sigma at
The tissues were then incubated with enhanced labelled polymer peroxidase
The reaction product of
antibody was visualized using diamino (DAB). The tissue sections were then counterstained with haematoxylin, followed by dehydrated through a of alcohols, and cleared with xylol. Finally, the
sections were mounted on glass slides with entelan, For the control of staining, tissue sections were incubated with instead of antibody. The tissue sections of control staining showed a negative reaction with background staining.
The distribution and frequency of positive
reaction of
staining on the tissues was observed qualitatively by means of light It was based on the brown colour intensity in the calls and the distribution of the reaction product.
Results and Discussion
The positive reaction
products
of immunohistochemically localization to the showed brown in the2008 August 19' - 2008
showed contain the enzyme. The
and
tissues, except in kidney glomerulus. medullacytoplasm
of
showed high content of adrenal gland, calls, andof These findings to the acinar cells of pancreatic tissues. function as a main gate for
It was well that the liver produce more
free by reactions In
mitochondria, and
detoxification
to the Kidney
tissues showed high content of Cu,
SOD especially In the proximal tubule cells, while distal tubule cells less
SOD, and there is no in the glomerulus. The tubule
cells
consistmore than other
part of and Makita,
so more reaction take In
the cells. The high content in the
may to prevent
from
all those danger free generated in the cells.In the adrenal tissues.
there
iscontent of in each part of the tissues. The highest content in the
zona followed by zona
zona respectively. Medulla
area of adrenal gland showed negative content
of testicle tissues showed
high content of in
and cells, less content in late spermatid cells, and there is no in
SOD
activity was also reported in and earlyof rat testis
al.,
1992). Heart tissues especially heart muscles showed high content of but some of muscles showed less It may to the degenerative processing of the cells. showed high levels in the Langerhans island oftissues, by contrast there is almost
no content of the enzyme in the acinar cells both nuclear and cytoplasm. It may to
the function of oxidations in a,
and
gama cells of island. These findings
also
showed that oxidationsmay
lesstake
place in acinar cells compared to the cellsin island.
The present study concluded
SOD was localized in the liver, kidney, adrenal gland, lung, testis, heart, and pancreatic
chemical 'of in the
(a) liver, (b), kidney, adrenal gland, lung, (e) testis, heart, and pancreatic tissues. Scale 25
References
B., and J.M.C. 1995. Free radicals
in
biology and medicine.Ed. Oxford : Press.
Wresdiyati, and 1995.
Remarkable increase of peroxisomes m
the renal tubule cells of Japanese
monkeys
under fasting stress.Wresdiyati T, RPA, Adnyane IKM, K. Irnmunohistochemical study of
(SOD) in the liver of diabetic
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