Occurance of sugarcane streak mosaic virus in Indonesia

(1)

OCCURANCE OF SUGARCANE STREAK MOSAI C VI RUS I N

I NDONESI A

Dr. Tri Asmira Damayanti

( Bogor Agricultural University)

Lilik Koesmihartono Putra, M.AgSt

(2)

Mosaic symptom (SCMV)

Streak mosaic symptom

Sampling of infected leaves

Java vs Outside Java

30% vs 67%

Sampling of infected leaves

Java vs outside Java

60% vs 30%

?

New strain of SCMV or new virus?

(3)

¾

Identify the streak mosaic causal, and its distribution

in Java

¾

Response commercial sugarcane clones against

streak mosaic disease

symptom variation

(4)

FI ELD SURVEY

PRI MARY DETECTI ON

TRANSMI SSI ON MODE

HOST RANGE TEST

DETECTI ON BY RT- PCR

DNA SEQUENCI NG

RESPONSE CLONES AGAI NST VI RUS

(5)

FIELD SURVEY

PEKALONGAN

YOGJAKARTA

SRAGEN

JAWA TI MUR

(6)

SURVEY RESULTS

4.09 0.00 0.00 23.38 19.34 11.97 13.66 1.31 0.00 4.95 2.59 PS 864 PS 862 SS- 57 PS 864 PS 864 PS 864 PS 864 BL PS 851 PS 864 PS 864 Klandungan I ( PC/ T)*

Klandungan I I ( PC/ T)*

Klandungan I I I ( PC/ T)*

Jambangan( R/ S)

Pilang( R/ S)

Purw osuman ( R/ S)

Kedungupit( R/ S)

Singopadu ( PC/ T)

Puro ( R/ T)

Karangudi ( R/ T)

Tanggulangin ( R/ T) 7.69 1.55 0.00 0.00 11.80 5.22 0.00 0.00 0.00 0.00 7.29 12.77 13.38 0.00 0.00 PS 921 PS 921 BL

PSCO 90- 2411

PS 864 PS 864 SS- 57 PS 851 PS 851 BL PS 864 PS 864 PS 864 PS 851 PS 951 Pesantren ( PC/ S)

Tegal Suruh ( PC/ S)

Krasak Ageng ( PC/ S)

Sijeruk B1 ( R/ S)

Sijeruk B2 ( R/ S)

Sumub Kidul ( PC/ S)

Sabar Wangi ( PC/ T)

Karang Tengah ( PC/ S)

Ampel Gading ( PC/ S)

Kebagusan B ( PC/ S)

Jatirejo ( PC/ S)

Karangbrai ( PC/ S)

Payung ( PC/ S)

Ujung Gede I ( PC/ S)*

Ujung Gede I I ( PC/ S)

I ( % ) Clone

Location ( Status/ ST) I ( % )

clone Location ( Status/ ST)

MOJO MI LL ( SRAGEN)

SRAGI MI LL ( PEKALONGAN)

(7)

SURVEY RESULTS

3.28 0.98 2.97 1.45 18.60 2.51 6.59 0.87 1.43 1.20 PS 864

PSCO 91- 787 PS 864PS 864 BM 96- 05 PS 864 PS 864 PS 864 PS 851 BL BL Beringin I ( PC/ S)

Beringin I I ( PC/ S) Jiken I ( PC/ S)*

Jiken I I ( PC/ S)*

Candipari ( PC/ S) Kebaron ( R/ S) Telasih ( PC/ S) Jedong 1 ( R/ T) Gempol ( PC/ T) Malangbong ( R/ T)

62.18 21.03 5.88 0.00 25.33 1.15 0.00 0.00 0.00 0.00 0.00 PS 864 PS 891 PS 862

PSCO 90- 2411 PS 864 PS 864 PA 198 PS 851 BL PS 862 PA 198 Masahan I ( PC/ S)*

Masahan I I ( PC/ S)*

Masahan I I I ( PC/ S)*

Masahan I V ( R/ S)*

Tempel ( R/ S) Ngaran ( PC/ S) Nggejik( PC/ S) Menjangan ( PC/ S) Pulutan( PC/ S) Jow ilayan ( PC/ S) Turi ( PC/ T)

I ( % )

Clone

Location

( Status/ ST)

I ( % )

Clone

Location

( Status/ ST)

TULANGAN MI LL ( SI DOARJO)

MADUKI SMO MI LL ( YOGJAKARTA)

(8)

SURVEY RESULTS

3.40

2.49

1.47

2.76

0.00

1.88

0.62

0.00

0.28

0.60

0.00

PS 921

BL

PS 921

PS 864

BL

PS 864

BL

CO 617

PSCO 90- 2411

PA 198

VMC 71- 39

PS 864

Wadung ( R/ S)

Bumiayu ( R/ S)

Wajak I ( R/ S)

Wajak I I ( R/ S)

Kuw olu ( PC/ T)

Sambigede ( PC/ S)

Pakishaji ( PC/ S)

Kebon Agung ( PC/ T)

Sitirejo I ( PC/ S)

Sitirejo I I ( PC/ S)

Sitirejo I I I ( PC/ S)

Sempalw adak ( R/ S)

I ( % )

Clone

Location ( Status/ ST)

KEBON AGUNG MI LL ( MALANG)

(9)

• Streak mosaic disease occurred in many

sugarcane plantations in Central and East Java

• The incidence was dominant in irrigated field/

paddy field than rain fed field

(10)

PRI MARY DETECTI ON

GENERAL POTYVI RUS

Antiserum (DSMZ)

SCMV (DSMZ)

NEGATI VE

RT-PCR Using SCMV universal primer S400-551 & S400-910

SEROLOGICAL

RT- PCR

SCMV A SCSMV L100

359 bp

(11)

I NOCULUM ( SAP)

Pad rubbing I noculation

TRANSMI SSI ON MODES

Via Cutting knife

(12)

S

y

m

p

to

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b

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m

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(13)

TRANSMI SSI ON MODES

-0

0

I nsect

a.

Rophalosiphum maydis

b.

Ceratovacuna lanigera

3.

+

100

Sett/ cutting cane

2.

-+

+

0

31

69

25

Mechanical inoculation

a. Healthy

b. Cutting knife

c. Sein’s method

d. Abrasive pad rubbing

e. Carborundum

1.

Result

I ncidence

( % )

Transmission mode

(14)

H

O

S

T

R

A

N

G

E

T

E

S

T

S

e

in

’s

M

e

th

o

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A

b

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iv

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p

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in

(15)

H

O

S

T

R

A

N

G

E

T

E

S

T

O

N

O

N

-G

R

A

M

I

N

A

E

P

L

A

N

T

(16)

O

B

S

E

R

V

A

T

I

O

N

&

S

A

M

P

L

I

N

(17)

-+ + -+ -MM SM -21-30 14-21 -L AMARANTHACEACE Amaranthus spinosus CHENOPODI ACEAE Chenopodium amaranticolor C. quinoa COMPOSI TAE Ageratum conyzoydes CUCURBI TACEAE Cucumis sativus GRAMI NAE Zea mays Sorghum bicolor LEGUMI NOSAE Arachis hypogea Phaseolus vulgaris Vigna unguiculata SOLANACEAE Lycopersicone esculentum Datura stramonium Physalis floridana Solanum melongena Nicotiana tabacum N. glutinosa

WEED GRAMI NAE

Cynodon dactilon Cynodon rotundus Penisetum purpureum Digitaria sp Echinochloa colonum Eleucine indica Dactylactonium aegypticum Results* Symptom I ncubation Period ( day) Famili/ Spesies

HOST RANGE

Narrow

L : latent symptom

SM : streak mosaic

MM : mild mosaic

* : detected by

(18)

DETECTI ON BY RT- PCR

100 200 300 400 500

SCSMV

DNA marker

Leaf samples

Total RNA extraction

cDNA Construction

(

reverse transcriptase

)

RT- PCR

AAAAA…

CP

NIb

NIa

VPg

6 K 2

CI

6 K 1

P3

HC-PRO

P1

AP3 SCSMV 547F

(19)

VI RAL PARTI CLES

PROTEI N ANALYSI S

SDS- PAGE

35 45

40 kDa

kDa 1 2

1. Unstained protein marker 2. SCSMV coat protein

Leaf Samples

Purification

Virion

(20)

GTGGGTTCAGTTCTCGGTTCGTAGCGGGAAACCCATAATACCACACAATGCAGTGAAGGCAGGATTGACCACACCAG AGTTTTATCCTTGCTGCATTGATTTCGTGATGGTGAATATCCTCTCACCAGCAGAAATTGACGTGCGTAACCAGGTGAT TAACGCACGTACACCCCGGATGGGAAAGCCTTTATTCCGTCATGCTCTTAGAGCCGGGGGAGATGAGGACACGGACC TGCGTAGGGAAGATGATGCAAACTATGGAAGGACGCAGATCGGTGGCGCTCATTTTGGGCGCGCCCAGCACTGATTT TCAGTGCAGTTATCCATATATTATCGTATCTACGTATCAACTGGTTCTCTTGGATAGGCTGATGGTAGTTGCCTGACCG CTTAGACGATCATCAGGAGCTACTAGTGGATCATTCGGAGATTGCTCACCCTGTTTCCAGACCAGTGAGATTCTGCCG -AACCCACAATCAACCTGCCCCGTGAGGAGGA

nt 547

nt 1045

AAAAA…

CP

NIb

NIa

VPg

6 K 2

CI

6 K 1

P3

HC-PRO

P1

AP3 SCSMV 547F

DNA SEQUENCES

The half 3’- CP gene had 98% homology with SCSMV-Pakistani

(Hall

et al

, 1998), 93.7% with -AP, 94.2% with –tn, and 94% with

(21)

Trial at experimental field belongs to I SRI Pasuruan, East Java

(22)

A.

B.

C.

Clearly Streak

(PS 864, SS 57, PS 921)

Moderate Streak

(PSBM 88-113, PSCO 90-2411,

PS 951, PS 851)

Mild Streak

(23)

9.02

5.44

40.50

15.05

9.57

7.21

16.94

4.72

30.77

23.30

PS 851

PS 862

PS 864

PS 921

PS 951

BL

PSJT 94- 33

PSCO 90- 2411

PSBM 88- 113

SS- 57

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

I ( % ) *

Clones

No

RESPONSE CLONES AGAI NST SCSMV- I dn I NFECTI ON

0% Highly resistant

0.1-5% Resistant (PSCO-90-2411)

5.1-10% Moderate (PS 851, PS 862, PS 951, BL)

10.1-40% Susceptible (PS 921, PSJT 94-33, PSBM 88-113, SS 57) > 40% Highly Susceptible (PS 864)

(24)

CONCLUSI ON

1. The streak mosaic

Sugarcane Streak Mosaic Virus.

Partially

alignment of CP gene show ed that SCSMV- I dn isolate had 98%

homology w ith SCSMV- Pkstn isolate.

2. SCSMV- I dn transmitted mechanically, and sett- borne but not

successfully transmitted by maize aphid

R. maydis

and mealybug

C.

lanigera

.

3. Cutting knife and sett- borne may play role in the spread of SCSMV

in the field.

4. Sorghum, maize and w eed

D. aegypticum

can be infected by SCSMV

5. All clones can be infected by SCSMV- I dn w ith three different symptom

such clearly, moderate and mild streak. I t depend on the type of clones

6. PSCO 90- 2411 w as resistant, PS 851, PS862, PS 951, BL w as moderate,

w hile PSJT 94- 33, PSBM 88- 113, PS 921 and SS 57 w as susceptible, and

(25)

Acknow ledgement

1. I ndonesian Ministry of agriculture for research funding

2. Competitive Based Program B of Dept. Plant Protection

Bogor Agricultural University & I ndonesian